[go: up one dir, main page]

RU2019138698A - Способы и композиции для днк-профилирования - Google Patents

Способы и композиции для днк-профилирования Download PDF

Info

Publication number
RU2019138698A
RU2019138698A RU2019138698A RU2019138698A RU2019138698A RU 2019138698 A RU2019138698 A RU 2019138698A RU 2019138698 A RU2019138698 A RU 2019138698A RU 2019138698 A RU2019138698 A RU 2019138698A RU 2019138698 A RU2019138698 A RU 2019138698A
Authority
RU
Russia
Prior art keywords
nucleic acid
acid sample
primers
snp
amplification
Prior art date
Application number
RU2019138698A
Other languages
English (en)
Other versions
RU2019138698A3 (ru
RU2752700C2 (ru
Inventor
Кэтрин М. СТЕФЕНС
Сидне ХОЛТ
Кэри ДЭВИС
Анне ЯГЕР
Паулина ВАЛИХЕВИЧ
Йонми ХАН
Дэвид СИЛВА
Минь-Цзюи Ричард ШЭНЬ
Сасан АМИНИ
Фрэнк СТИМЕРС
Original Assignee
Иллумина, Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Иллумина, Инк. filed Critical Иллумина, Инк.
Publication of RU2019138698A publication Critical patent/RU2019138698A/ru
Publication of RU2019138698A3 publication Critical patent/RU2019138698A3/ru
Application granted granted Critical
Publication of RU2752700C2 publication Critical patent/RU2752700C2/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2522/00—Reaction characterised by the use of non-enzymatic proteins
    • C12Q2522/10—Nucleic acid binding proteins
    • C12Q2522/101—Single or double stranded nucleic acid binding proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/204—Modifications characterised by specific length of the oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)

Claims (23)

1. Способ получения ДНК–профиля, включающий
обеспечение образца нуклеиновой кислоты,
амплификацию образца нуклеиновой кислоты с множеством праймеров, которые специфически гибридизуются по меньшей мере с одной последовательностью-мишенью, содержащей однонуклеотидный полиморфизм (SNP), и по меньшей мере с одной последовательностью-мишенью, содержащей тандемный повтор, в мультиплексной реакции с получением продуктов амплификации, где по меньшей мере один из множества праймеров имеет низкую температуру плавления, имеет длину по меньшей мере 24 нуклеотида или содержит гомополимерную нуклеотидную последовательность, или их комбинацию, и
определение в продуктах амплификации генотипов по меньшей мере одного SNP и по меньшей мере одного тандемного повтора, таким образом, получая ДНК-профиль образца нуклеиновой кислоты.
2. Способ по п.1, в котором образец нуклеиновой кислоты получают у человека.
3. Способ по п.1, в котором по меньшей мере один SNP включает SNP, который, как известно, указывает на родственную или фенотипическую характеристику источника образца нуклеиновой кислоты.
4. Способ по п. 1, в котором по меньшей мере один из множества праймеров содержит по меньшей мере 24 нуклеотида.
5. Способ по п.4, в котором по меньшей мере один из множества праймеров имеет температуру плавления менее 60°С.
6. Способ по п.1, в котором амплификация образца нуклеиновой кислоты включает проведение полимеразной цепной реакции (ПЦР) на образце нуклеиновой кислоты.
7. Способ по п.1, в котором множество праймеров специфически гибридизуются по меньшей мере с 30 SNP.
8. Способ по п.1, в котором множество праймеров специфически гибридизуются по меньшей мере с 24 последовательностями тандемных повторов.
9. Способ по п.1, в котором образец нуклеиновой кислоты содержит геномную ДНК.
10. Способ по п.9, в котором обеспечение образца нуклеиновой кислоты включает обеспечение криминалистического образца, содержащего нуклеиновую кислоту.
11. Способ по п.1, в котором определение генотипов включает определение по меньшей мере 90% генотипов по меньшей мере одного SNP и по меньшей мере одного тандемного повтора.
12. Способ по п.1, в котором, по меньшей мере, один из множества праймеров содержит одну или несколько последовательностей–меток, содержащих метку уникального молекулярного идентификатора.
13. Способ построения библиотеки нуклеиновых кислот, включающий: предоставление образца нуклеиновой кислоты и амплификацию образца нуклеиновой кислоты со множеством праймеров, которые специфически гибридизуются с по меньшей мере одним однонуклеотидным полиморфизмом (SNP) и по меньшей мере с одной последовательностью-мишенью, содержащей последовательность тандемного повтора в мультиплексной реакции для получения продуктов амплификации, где по меньшей мере один из множества праймеров имеет низкую температуру плавления, имеет длину по меньшей мере 24 нуклеотида или содержит гомополимерную нуклеотидную последовательность, или их комбинацию.
14. Способ по п.13, в котором по меньшей мере один SNP указывает на родственную или фенотипическую характеристику источника образца нуклеиновой кислоты.
15. Способ по п.13, в котором по меньшей мере один из множества праймеров содержит одну или несколько последовательностей-меток.
16. Способ по п.15, в котором одна или несколько последовательностей-меток содержат метку-праймер, метку для захвата, метку для секвенирования или метку уникального молекулярного идентификатора или их сочетание.
17. Способ по п.16, дополнительно включающий амплификацию продуктов амплификации со вторым множеством праймеров.
18. Способ по п.17, в котором по меньшей мере один из второго множества праймеров содержит часть, соответствующую метке-праймеру из множества праймеров и одной или нескольким последовательностям-меткам.
19. Способ по п.17, включающий добавление связывающего одноцепочечные ДНК белка (SSB) к продуктам амплификации.
20. Способ по п.13, в котором амплификация образца нуклеиновой кислоты включает амплификацию посредством полимеразной цепной реакции (ПЦР).
RU2019138698A 2014-02-18 2015-02-13 Способы и композиции для днк-профилирования RU2752700C2 (ru)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201461940942P 2014-02-18 2014-02-18
US61/940,942 2014-02-18
US201462043060P 2014-08-28 2014-08-28
US62/043,060 2014-08-28
US201562103524P 2015-01-14 2015-01-14
US62/103,524 2015-01-14

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
RU2016133987A Division RU2708337C2 (ru) 2014-02-18 2015-02-13 Способы и композиции для днк-профилирования

Publications (3)

Publication Number Publication Date
RU2019138698A true RU2019138698A (ru) 2020-01-27
RU2019138698A3 RU2019138698A3 (ru) 2020-06-19
RU2752700C2 RU2752700C2 (ru) 2021-07-30

Family

ID=52629678

Family Applications (2)

Application Number Title Priority Date Filing Date
RU2016133987A RU2708337C2 (ru) 2014-02-18 2015-02-13 Способы и композиции для днк-профилирования
RU2019138698A RU2752700C2 (ru) 2014-02-18 2015-02-13 Способы и композиции для днк-профилирования

Family Applications Before (1)

Application Number Title Priority Date Filing Date
RU2016133987A RU2708337C2 (ru) 2014-02-18 2015-02-13 Способы и композиции для днк-профилирования

Country Status (22)

Country Link
US (2) US10422002B2 (ru)
EP (2) EP3108009B1 (ru)
JP (3) JP7011392B2 (ru)
CN (1) CN106164298B (ru)
AU (2) AU2015219289B2 (ru)
BR (2) BR112016019267B8 (ru)
CA (1) CA2940048C (ru)
DK (1) DK3108009T3 (ru)
ES (1) ES2866044T3 (ru)
HR (1) HRP20210953T1 (ru)
HU (1) HUE055256T2 (ru)
LT (1) LT3108009T (ru)
MX (1) MX379233B (ru)
MY (2) MY200537A (ru)
NZ (1) NZ723399A (ru)
PL (1) PL3108009T3 (ru)
PT (1) PT3108009T (ru)
RS (1) RS61976B1 (ru)
RU (2) RU2708337C2 (ru)
SA (1) SA516371691B1 (ru)
SI (1) SI3108009T1 (ru)
WO (1) WO2015126766A1 (ru)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2015144287A (ru) * 2013-03-15 2017-04-24 Эббви Дойчланд Гмбх Унд Ко. Кг Составы конъюгата антитело против egfr-лекарственное средство
MY200537A (en) * 2014-02-18 2024-01-02 Illumina Inc Methods and compositions for dna profiling
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US9859394B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
EP3235010A4 (en) 2014-12-18 2018-08-29 Agilome, Inc. Chemically-sensitive field effect transistor
US9618474B2 (en) 2014-12-18 2017-04-11 Edico Genome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
EP3286338B1 (en) * 2015-04-24 2023-11-01 Atila Biosystems Incorporated Amplification with primers of limited nucleotide composition
CN105755129B (zh) * 2016-03-21 2020-03-31 北京市理化分析测试中心 基于新一代测序的基因座d8s1179的str分型方法
WO2017196720A1 (en) * 2016-05-13 2017-11-16 Colorado State University Research Foundation High throughput method to genotype plants
US10811539B2 (en) 2016-05-16 2020-10-20 Nanomedical Diagnostics, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10822647B2 (en) * 2016-07-12 2020-11-03 Biodynamics S.R.L. Methods for using long ssDNA polynucleotides as primers (superprimers) in PCR assays
IL266197B2 (en) * 2016-10-24 2024-03-01 Geneinfosec Inc Hiding information contained within nucleic acids
WO2018175997A1 (en) * 2017-03-23 2018-09-27 University Of Washington Methods for targeted nucleic acid sequence enrichment with applications to error corrected nucleic acid sequencing
CN106835292B (zh) * 2017-04-05 2019-04-09 北京泛生子基因科技有限公司 一步法快速构建扩增子文库的方法
EP4345159A3 (en) 2017-05-05 2024-06-05 Scipio Bioscience Methods for trapping and barcoding discrete biological units in hydrogel
WO2018217912A1 (en) * 2017-05-23 2018-11-29 President And Fellows Of Harvard College Multiplex end-tagging amplification of nucleic acids
EP3642362B1 (en) 2017-06-20 2025-10-15 Illumina, Inc. Methods for addressing inefficiencies in amplification reactions
JP7537746B2 (ja) 2017-08-11 2024-08-21 アティラ バイオシステムズ インコーポレイテッド ヌクレオチド組成が限定されたプライマーを用いたデジタル増幅
CN107868837B (zh) * 2017-12-12 2019-03-01 苏州普瑞森基因科技有限公司 一种用于分析肠道微生物的引物组合物及其应用
CN109988826A (zh) * 2017-12-30 2019-07-09 安诺优达基因科技(北京)有限公司 一种基于str计算混合样本嵌合比例的方法以及系统
EP3517629A1 (en) * 2018-01-30 2019-07-31 Myway Genetics S.r.L. Use of cfdna fragments as biomarkers in patients after organ transplantation
CN108342469A (zh) * 2018-02-27 2018-07-31 广州中安基因科技有限公司 一种皮肤基因检测的基因芯片
AU2019240244A1 (en) 2018-03-22 2020-11-19 President And Fellows Of Harvard College Methods and compositions for molecular authentication
JP6646120B1 (ja) * 2018-10-01 2020-02-14 日本ソフトウェアマネジメント株式会社 分解されたｄｎａで個体識別可能なｄｎａ鑑定方法
KR102097721B1 (ko) * 2019-01-24 2020-04-06 주식회사 시선바이오머티리얼스 태그서열 snp를 이용한 단일 검출 프로브 기반 다중 표적 검출방법
CN111485024B (zh) * 2019-01-29 2024-04-26 深圳华大法医科技有限公司 用于个体特征确认的引物组合、及其应用
KR102351579B1 (ko) * 2019-06-25 2022-01-17 대한민국 상염색체 str 좌위를 포함한 ngs 패널 및 다중증폭 시스템
AU2020345028A1 (en) * 2019-09-11 2022-03-10 Parlanca Limited A multiplex method of preparing a sequencing library
KR20220160633A (ko) * 2020-03-31 2022-12-06 퍼스널 게놈 다이아그노스틱스 인코포레이티드 핵산 품질 결정을 위한 조성물 및 방법
CN112592981B (zh) * 2020-12-01 2023-06-20 广州精科医学检验所有限公司 用于dna档案建库的引物组、试剂盒和方法
CN112342303A (zh) * 2020-12-04 2021-02-09 郑州高新生物技术有限公司 一种基于ngs的人类y染色体str和snp遗传标记联合检测体系及检测方法
CN112852970A (zh) * 2020-12-31 2021-05-28 百特元生物科技（北京）有限公司 一种同时扩增人37个y-str基因座的引物组、试剂盒及其应用
JP2024507168A (ja) 2021-02-12 2024-02-16 ベロジェン・インコーポレイテッド Ｄｎａベースの血縁関係解析のための方法および組成物
CN113293205B (zh) * 2021-05-24 2024-11-26 深圳市真迈生物科技有限公司 测序方法
KR102337267B1 (ko) * 2021-06-01 2021-12-08 주식회사 다우진유전자연구소 유전자 감식을 위한 24개의 str 유전자 마커를 이용한 pcr 다중증폭 시스템
CN113930490B (zh) * 2021-09-27 2024-09-10 江汉大学 二代测序平台中利用分子特异性条形码评估str滑脱的方法及其应用
EP4416734A4 (en) * 2021-10-13 2025-08-27 Verogen Inc METHODS AND COMPOSITIONS FOR IMPROVING THE ACCURACY OF DNA-BASED KINSHIP ANALYSIS
EP4272764A1 (en) 2022-05-03 2023-11-08 Scipio Bioscience Method of complexing biological units with particles
JP2025530659A (ja) 2022-08-16 2025-09-17 ベロジェン・インコーポレイテッド 行方不明者および災害／紛争犠牲者のための血縁関係評価のための方法およびシステム
WO2024086805A2 (en) * 2022-10-21 2024-04-25 Watchmaker Genomics, Inc. Methods and compositions for sequencing library normalization
EP4603595A1 (en) * 2024-02-13 2025-08-20 ImmuneDiscover Sweden AB A method for typing the immune genes and the allelic variants thereof

Family Cites Families (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0450060A1 (en) 1989-10-26 1991-10-09 Sri International Dna sequencing
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
JP2002503954A (ja) 1997-04-01 2002-02-05 グラクソ、グループ、リミテッド 核酸増幅法
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
GB9929381D0 (en) * 1999-12-10 2000-02-09 Pyrosequencing Ab A method of assessing the amount of nucleic acid in a sample
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
CN101525660A (zh) 2000-07-07 2009-09-09 维西根生物技术公司 实时序列测定
EP1354064A2 (en) 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US7078168B2 (en) * 2001-02-27 2006-07-18 Biotage Ab Method for determining allele frequencies
JP4210769B2 (ja) 2001-11-22 2009-01-21 独立行政法人産業技術総合研究所 Ｇａｔｅｗａｙエントリークローンの作製方法
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
EP3795577A1 (en) 2002-08-23 2021-03-24 Illumina Cambridge Limited Modified nucleotides
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
EP3175914A1 (en) 2004-01-07 2017-06-07 Illumina Cambridge Limited Improvements in or relating to molecular arrays
US20060014190A1 (en) * 2004-06-30 2006-01-19 Hennessy Lori K Methods for analyzing short tandem repeats and single nucleotide polymorphisms
US20060057595A1 (en) * 2004-09-16 2006-03-16 Applera Corporation Compositions, methods, and kits for identifying and quantitating small RNA molecules
US7315019B2 (en) 2004-09-17 2008-01-01 Pacific Biosciences Of California, Inc. Arrays of optical confinements and uses thereof
EP1828412B2 (en) 2004-12-13 2019-01-09 Illumina Cambridge Limited Improved method of nucleotide detection
US8623628B2 (en) 2005-05-10 2014-01-07 Illumina, Inc. Polymerases
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
EP3722409A1 (en) 2006-03-31 2020-10-14 Illumina, Inc. Systems and devices for sequence by synthesis analysis
WO2008111990A1 (en) * 2006-06-14 2008-09-18 Cellpoint Diagnostics, Inc. Rare cell analysis using sample splitting and dna tags
WO2008051530A2 (en) 2006-10-23 2008-05-02 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
EP4134667B1 (en) 2006-12-14 2025-11-12 Life Technologies Corporation Apparatus for measuring analytes using fet arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US9434997B2 (en) * 2007-08-24 2016-09-06 Lawrence Livermore National Security, Llc Methods, compounds and systems for detecting a microorganism in a sample
US20090163366A1 (en) * 2007-12-24 2009-06-25 Helicos Biosciences Corporation Two-primer sequencing for high-throughput expression analysis
US8709726B2 (en) * 2008-03-11 2014-04-29 Sequenom, Inc. Nucleic acid-based tests for prenatal gender determination
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
CA2786564A1 (en) 2010-01-19 2011-07-28 Verinata Health, Inc. Identification of polymorphic sequences in mixtures of genomic dna by whole genome sequencing
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
CA2835620C (en) 2011-05-12 2021-02-23 Netbio, Inc. Methods and compositions for rapid multiplex amplification of str loci
CA2859660C (en) 2011-09-23 2021-02-09 Illumina, Inc. Methods and compositions for nucleic acid sequencing
JP6159391B2 (ja) 2012-04-03 2017-07-05 イラミーナ インコーポレーテッド 核酸シークエンシングに有用な統合化した読取りヘッド及び流体カートリッジ
JP6382189B2 (ja) 2012-06-29 2018-08-29 ゼネラル・エレクトリック・カンパニイ Ｒｎａテンプレートから開始する等温ｄｎａ増幅用のキット
MY200537A (en) * 2014-02-18 2024-01-02 Illumina Inc Methods and compositions for dna profiling

Also Published As

Publication number Publication date
RS61976B1 (sr) 2021-07-30
CA2940048A1 (en) 2016-08-27
PT3108009T (pt) 2021-05-10
SA516371691B1 (ar) 2022-03-20
AU2021203877B2 (en) 2023-11-23
RU2019138698A3 (ru) 2020-06-19
NZ761724A (en) 2024-04-26
JP2024059651A (ja) 2024-05-01
JP2017509329A (ja) 2017-04-06
HRP20210953T1 (hr) 2021-09-03
PL3108009T3 (pl) 2021-10-11
MX2016010717A (es) 2017-04-27
JP2021177777A (ja) 2021-11-18
RU2016133987A (ru) 2018-03-27
AU2021203877A1 (en) 2021-07-08
MY200537A (en) 2024-01-02
NZ723399A (en) 2020-08-28
US20200224268A1 (en) 2020-07-16
MY182415A (en) 2021-01-25
EP3910069A1 (en) 2021-11-17
MX379233B (es) 2025-03-10
HUE055256T2 (hu) 2021-11-29
JP7770437B2 (ja) 2025-11-14
RU2016133987A3 (ru) 2018-12-19
BR122022007092B1 (pt) 2022-07-19
AU2015219289B2 (en) 2021-05-06
BR112016019267A2 (pt) 2017-10-10
WO2015126766A1 (en) 2015-08-27
LT3108009T (lt) 2021-07-12
DK3108009T3 (da) 2021-06-07
RU2752700C2 (ru) 2021-07-30
US11530446B2 (en) 2022-12-20
CN106164298A (zh) 2016-11-23
AU2015219289A1 (en) 2016-09-08
WO2015126766A8 (en) 2016-11-03
US10422002B2 (en) 2019-09-24
BR122022007092B8 (pt) 2023-01-31
BR112016019267B1 (pt) 2022-06-28
US20150232929A1 (en) 2015-08-20
ES2866044T3 (es) 2021-10-19
EP3108009B1 (en) 2021-03-24
JP7011392B2 (ja) 2022-01-26
RU2708337C2 (ru) 2019-12-05
SI3108009T1 (sl) 2021-09-30
EP3108009A1 (en) 2016-12-28
CA2940048C (en) 2023-03-14
CN106164298B (zh) 2020-02-21
BR112016019267B8 (pt) 2023-01-10

Similar Documents

Publication Publication Date Title
RU2019138698A (ru) Способы и композиции для днк-профилирования
JP2024059651A5 (ru)
JP2015156872A5 (ru)
WO2007130519A3 (en) Viral nucleic acid microarray and method of use
JP2013055956A5 (ru)
JP2018514230A5 (ru)
JP2016195614A5 (ru)
WO2005089508A3 (en) Dna sequence detection by limited primer extension
WO2014012107A3 (en) Human identifiation using a panel of snps
WO2016154337A3 (en) Method for identification and enumeration of nucleic acid sequences
ATE516371T1 (de) Oligonukleotide und deren verwendungen
JP2016500275A5 (ru)
JP2011101652A5 (ru)
JP2014083053A5 (ru)
JP2016539624A5 (ru)
RU2014115802A (ru) Зонд со специфичностью к нескольким целевым областям, состоящий из трех частей
JP2016512437A5 (ru)
JP2017522015A5 (ru)
DE60335116D1 (de) Verfahren zum nachweis von sequenzunterschieden
JP2014030431A5 (ru)
MY184632A (en) A method of identifying parentage in freshwater prawn macrobrachium rosenbergii
JP2013515458A5 (ru)
RU2018113750A (ru) Улучшенное выявление коротких гомополимерных повторов
US20170029886A1 (en) High-resolution hla typing
JP2016524906A5 (ru)