MY184632A - A method of identifying parentage in freshwater prawn macrobrachium rosenbergii - Google Patents
A method of identifying parentage in freshwater prawn macrobrachium rosenbergiiInfo
- Publication number
- MY184632A MY184632A MYPI2013701608A MYPI2013701608A MY184632A MY 184632 A MY184632 A MY 184632A MY PI2013701608 A MYPI2013701608 A MY PI2013701608A MY PI2013701608 A MYPI2013701608 A MY PI2013701608A MY 184632 A MY184632 A MY 184632A
- Authority
- MY
- Malaysia
- Prior art keywords
- seq
- macrobrachium rosenbergii
- parentage
- identifying
- amplicons
- Prior art date
Links
- 241001327110 Macrobrachium rosenbergii Species 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 239000013505 freshwater Substances 0.000 title 1
- 108091093088 Amplicon Proteins 0.000 abstract 3
- 108700028369 Alleles Proteins 0.000 abstract 2
- 239000012634 fragment Substances 0.000 abstract 2
- 241000238557 Decapoda Species 0.000 abstract 1
- 239000012472 biological sample Substances 0.000 abstract 1
- 150000007523 nucleic acids Chemical group 0.000 abstract 1
- 238000003752 polymerase chain reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/124—Animal traits, i.e. production traits, including athletic performance or the like
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
A method of identifying parentage in Macrobrachium rosenbergii prawn comprising the steps of amplifying targeted EST-SSR nucleic acid sequences from a biological sample of Macrobrachium rosenbergii through a polymerase chain reaction using primer pairs with nucleic acid sequences as set forth in SEQ ID NO. 1 and SEQ ID NO. 2, SEQ ID NO. 3 and SEQ ID NO. 4, SEQ ID NO. 5 and SEQ ID NO. 6, SEQ ID NO. 7 and SEQ ID NO. 8, SEQ ID NO. 9 and SEQ ID NO. 10, SEQ ID NO. 11 and SEQ ID NO. 12 to produce respective amplicons; determining fragment size of the produced amplicons; regarding the fragment size of the amplicons corresponding to predetermined alleles; and identifying possible parentage of the prawn based on the regarded allele.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MYPI2013701608A MY184632A (en) | 2013-09-09 | 2013-09-09 | A method of identifying parentage in freshwater prawn macrobrachium rosenbergii |
| PCT/MY2014/000229 WO2015034347A1 (en) | 2013-09-09 | 2014-09-09 | A method of identifying parentage in freshwater prawn macrobrachium rosenbergii |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MYPI2013701608A MY184632A (en) | 2013-09-09 | 2013-09-09 | A method of identifying parentage in freshwater prawn macrobrachium rosenbergii |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MY184632A true MY184632A (en) | 2021-04-12 |
Family
ID=52628707
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MYPI2013701608A MY184632A (en) | 2013-09-09 | 2013-09-09 | A method of identifying parentage in freshwater prawn macrobrachium rosenbergii |
Country Status (2)
| Country | Link |
|---|---|
| MY (1) | MY184632A (en) |
| WO (1) | WO2015034347A1 (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105349651B (en) * | 2015-11-18 | 2018-05-04 | 广东省中药研究所 | The method and primer of EST-SSR Marker Identification Chinese medicine rabdosia lophanthide kinds |
| CN109797226A (en) * | 2019-02-26 | 2019-05-24 | 中国水产科学研究院珠江水产研究所 | A kind of Macrobrachium rosenbergii classification method based on EST-SSR label |
| CN110016510B (en) * | 2019-04-22 | 2022-06-07 | 宁波大学 | A Molecular Marker for Genetic Selection of Macrobrachium rosenbergii |
| CN111996261B (en) * | 2020-07-31 | 2023-04-28 | 湖南银鱼农业科技有限公司 | Macrobrachium rosenbergii sex molecular marker primer and application thereof |
| CN113881782A (en) * | 2021-10-25 | 2022-01-04 | 上海海洋大学 | Primer group, kit, identification method and application for identifying sex of macrobrachium rosenbergii |
| CN114395635B (en) * | 2022-03-11 | 2023-07-28 | 广西壮族自治区水产科学研究院 | SNP molecular marker related to growth traits of macrobrachium rosenbergii and application of SNP molecular marker |
| CN114561479B (en) * | 2022-03-25 | 2023-03-17 | 浙江省淡水水产研究所 | A kind of primer and its application for identification of individual clams in Macrobrachium rosenbergii |
-
2013
- 2013-09-09 MY MYPI2013701608A patent/MY184632A/en unknown
-
2014
- 2014-09-09 WO PCT/MY2014/000229 patent/WO2015034347A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO2015034347A1 (en) | 2015-03-12 |
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