MX2016010717A - Métodos y composiciones para el perfilado de adn. - Google Patents
Métodos y composiciones para el perfilado de adn.Info
- Publication number
- MX2016010717A MX2016010717A MX2016010717A MX2016010717A MX2016010717A MX 2016010717 A MX2016010717 A MX 2016010717A MX 2016010717 A MX2016010717 A MX 2016010717A MX 2016010717 A MX2016010717 A MX 2016010717A MX 2016010717 A MX2016010717 A MX 2016010717A
- Authority
- MX
- Mexico
- Prior art keywords
- nucleic acid
- acid sample
- target sequence
- primers
- amplification
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 239000000203 mixture Substances 0.000 title 1
- 230000003321 amplification Effects 0.000 abstract 5
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 5
- 150000007523 nucleic acids Chemical class 0.000 abstract 5
- 102000039446 nucleic acids Human genes 0.000 abstract 5
- 108020004707 nucleic acids Proteins 0.000 abstract 5
- 239000003550 marker Substances 0.000 abstract 1
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
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- Immunology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Saccharide Compounds (AREA)
Abstract
Las realizaciones desveladas en el presente documento proporcionan métodos para construir un perfil de ADN que comprende: una muestra de ácido nucleico, la amplificación de la muestra de ácido nucleico con una pluralidad de cebadores que hibridan específicamente con al menos una secuencia diana que comprende un polimorfismo mononucleotídico (SNP) y al menos una secuencia diana que comprende una repetición en tándem, y determinar los genotipos de dicho al menos un SNP y de dicha al menos una repetición en tándem en los productos de amplificación, construyéndose de esta manera el perfil de ADN de la muestra de ácido nucleico. Las realizaciones desveladas en el presente documento proporcionan además, una pluralidad de cebadores que hibridan específicamente con al menos una secuencia diana corta y al menos una secuencia diana larga en una muestra de ácido nucleico, en la que la amplificación de la muestra de ácido nucleico usando la pluralidad de cebadores en una sola reacción da como resultado al menos un producto de amplificación corto y al menos un producto de amplificación largo, en la que cada uno de la pluralidad de cebadores comprende una o más secuencias marcadoras.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461940942P | 2014-02-18 | 2014-02-18 | |
| US201462043060P | 2014-08-28 | 2014-08-28 | |
| US201562103524P | 2015-01-14 | 2015-01-14 | |
| PCT/US2015/015939 WO2015126766A1 (en) | 2014-02-18 | 2015-02-13 | Methods and compositions for dna profiling |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2016010717A true MX2016010717A (es) | 2017-04-27 |
| MX379233B MX379233B (es) | 2025-03-10 |
Family
ID=52629678
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2016010717A MX379233B (es) | 2014-02-18 | 2015-02-13 | Métodos y composiciones para el perfilado de adn. |
Country Status (22)
| Country | Link |
|---|---|
| US (2) | US10422002B2 (es) |
| EP (2) | EP3108009B1 (es) |
| JP (3) | JP7011392B2 (es) |
| CN (1) | CN106164298B (es) |
| AU (2) | AU2015219289B2 (es) |
| BR (2) | BR112016019267B8 (es) |
| CA (1) | CA2940048C (es) |
| DK (1) | DK3108009T3 (es) |
| ES (1) | ES2866044T3 (es) |
| HR (1) | HRP20210953T1 (es) |
| HU (1) | HUE055256T2 (es) |
| LT (1) | LT3108009T (es) |
| MX (1) | MX379233B (es) |
| MY (2) | MY200537A (es) |
| NZ (1) | NZ723399A (es) |
| PL (1) | PL3108009T3 (es) |
| PT (1) | PT3108009T (es) |
| RS (1) | RS61976B1 (es) |
| RU (2) | RU2708337C2 (es) |
| SA (1) | SA516371691B1 (es) |
| SI (1) | SI3108009T1 (es) |
| WO (1) | WO2015126766A1 (es) |
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| RU2015144287A (ru) * | 2013-03-15 | 2017-04-24 | Эббви Дойчланд Гмбх Унд Ко. Кг | Составы конъюгата антитело против egfr-лекарственное средство |
| MY200537A (en) * | 2014-02-18 | 2024-01-02 | Illumina Inc | Methods and compositions for dna profiling |
| US10020300B2 (en) | 2014-12-18 | 2018-07-10 | Agilome, Inc. | Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids |
| US9859394B2 (en) | 2014-12-18 | 2018-01-02 | Agilome, Inc. | Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids |
| US10006910B2 (en) | 2014-12-18 | 2018-06-26 | Agilome, Inc. | Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same |
| EP3235010A4 (en) | 2014-12-18 | 2018-08-29 | Agilome, Inc. | Chemically-sensitive field effect transistor |
| US9618474B2 (en) | 2014-12-18 | 2017-04-11 | Edico Genome, Inc. | Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids |
| US9857328B2 (en) | 2014-12-18 | 2018-01-02 | Agilome, Inc. | Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same |
| EP3286338B1 (en) * | 2015-04-24 | 2023-11-01 | Atila Biosystems Incorporated | Amplification with primers of limited nucleotide composition |
| CN105755129B (zh) * | 2016-03-21 | 2020-03-31 | 北京市理化分析测试中心 | 基于新一代测序的基因座d8s1179的str分型方法 |
| WO2017196720A1 (en) * | 2016-05-13 | 2017-11-16 | Colorado State University Research Foundation | High throughput method to genotype plants |
| US10811539B2 (en) | 2016-05-16 | 2020-10-20 | Nanomedical Diagnostics, Inc. | Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids |
| US10822647B2 (en) * | 2016-07-12 | 2020-11-03 | Biodynamics S.R.L. | Methods for using long ssDNA polynucleotides as primers (superprimers) in PCR assays |
| IL266197B2 (en) * | 2016-10-24 | 2024-03-01 | Geneinfosec Inc | Hiding information contained within nucleic acids |
| WO2018175997A1 (en) * | 2017-03-23 | 2018-09-27 | University Of Washington | Methods for targeted nucleic acid sequence enrichment with applications to error corrected nucleic acid sequencing |
| CN106835292B (zh) * | 2017-04-05 | 2019-04-09 | 北京泛生子基因科技有限公司 | 一步法快速构建扩增子文库的方法 |
| EP4345159A3 (en) | 2017-05-05 | 2024-06-05 | Scipio Bioscience | Methods for trapping and barcoding discrete biological units in hydrogel |
| WO2018217912A1 (en) * | 2017-05-23 | 2018-11-29 | President And Fellows Of Harvard College | Multiplex end-tagging amplification of nucleic acids |
| EP3642362B1 (en) | 2017-06-20 | 2025-10-15 | Illumina, Inc. | Methods for addressing inefficiencies in amplification reactions |
| JP7537746B2 (ja) | 2017-08-11 | 2024-08-21 | アティラ バイオシステムズ インコーポレイテッド | ヌクレオチド組成が限定されたプライマーを用いたデジタル増幅 |
| CN107868837B (zh) * | 2017-12-12 | 2019-03-01 | 苏州普瑞森基因科技有限公司 | 一种用于分析肠道微生物的引物组合物及其应用 |
| CN109988826A (zh) * | 2017-12-30 | 2019-07-09 | 安诺优达基因科技(北京)有限公司 | 一种基于str计算混合样本嵌合比例的方法以及系统 |
| EP3517629A1 (en) * | 2018-01-30 | 2019-07-31 | Myway Genetics S.r.L. | Use of cfdna fragments as biomarkers in patients after organ transplantation |
| CN108342469A (zh) * | 2018-02-27 | 2018-07-31 | 广州中安基因科技有限公司 | 一种皮肤基因检测的基因芯片 |
| AU2019240244A1 (en) | 2018-03-22 | 2020-11-19 | President And Fellows Of Harvard College | Methods and compositions for molecular authentication |
| JP6646120B1 (ja) * | 2018-10-01 | 2020-02-14 | 日本ソフトウェアマネジメント株式会社 | 分解されたdnaで個体識別可能なdna鑑定方法 |
| KR102097721B1 (ko) * | 2019-01-24 | 2020-04-06 | 주식회사 시선바이오머티리얼스 | 태그서열 snp를 이용한 단일 검출 프로브 기반 다중 표적 검출방법 |
| CN111485024B (zh) * | 2019-01-29 | 2024-04-26 | 深圳华大法医科技有限公司 | 用于个体特征确认的引物组合、及其应用 |
| KR102351579B1 (ko) * | 2019-06-25 | 2022-01-17 | 대한민국 | 상염색체 str 좌위를 포함한 ngs 패널 및 다중증폭 시스템 |
| AU2020345028A1 (en) * | 2019-09-11 | 2022-03-10 | Parlanca Limited | A multiplex method of preparing a sequencing library |
| KR20220160633A (ko) * | 2020-03-31 | 2022-12-06 | 퍼스널 게놈 다이아그노스틱스 인코포레이티드 | 핵산 품질 결정을 위한 조성물 및 방법 |
| CN112592981B (zh) * | 2020-12-01 | 2023-06-20 | 广州精科医学检验所有限公司 | 用于dna档案建库的引物组、试剂盒和方法 |
| CN112342303A (zh) * | 2020-12-04 | 2021-02-09 | 郑州高新生物技术有限公司 | 一种基于ngs的人类y染色体str和snp遗传标记联合检测体系及检测方法 |
| CN112852970A (zh) * | 2020-12-31 | 2021-05-28 | 百特元生物科技(北京)有限公司 | 一种同时扩增人37个y-str基因座的引物组、试剂盒及其应用 |
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