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SK752686A3 - Activation method of by genetic technology gained heterologous proteins with content of disulfidic bridges of eukaryotic origin after expression in prokaryotic organisms - Google Patents

Activation method of by genetic technology gained heterologous proteins with content of disulfidic bridges of eukaryotic origin after expression in prokaryotic organisms Download PDF

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Publication number
SK752686A3
SK752686A3 SK7526-86A SK752686A SK752686A3 SK 752686 A3 SK752686 A3 SK 752686A3 SK 752686 A SK752686 A SK 752686A SK 752686 A3 SK752686 A3 SK 752686A3
Authority
SK
Slovakia
Prior art keywords
activation
mol
concentration
mmol
carried out
Prior art date
Application number
SK7526-86A
Other languages
English (en)
Slovak (sk)
Other versions
SK278317B6 (en
Inventor
Rudolph Rainer
Stephan Fischer
Ralf Mattes
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Publication of SK278317B6 publication Critical patent/SK278317B6/sk
Publication of SK752686A3 publication Critical patent/SK752686A3/sk

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)
  • Lubricants (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
SK7526-86A 1985-10-23 1986-10-17 Activation method of by genetic technology gained heterologous proteins with content of disulfidic bridges of eukaryotic origin after expression in prokaryotic organisms SK752686A3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19853537708 DE3537708A1 (de) 1985-10-23 1985-10-23 Verfahren zur aktivierung von t-pa nach expression in prokaryonten

Publications (2)

Publication Number Publication Date
SK278317B6 SK278317B6 (en) 1996-10-02
SK752686A3 true SK752686A3 (en) 1996-10-02

Family

ID=6284269

Family Applications (1)

Application Number Title Priority Date Filing Date
SK7526-86A SK752686A3 (en) 1985-10-23 1986-10-17 Activation method of by genetic technology gained heterologous proteins with content of disulfidic bridges of eukaryotic origin after expression in prokaryotic organisms

Country Status (26)

Country Link
EP (3) EP0253823A1 (fr)
JP (2) JPH0728745B2 (fr)
KR (1) KR900009139B1 (fr)
AT (2) ATE131489T1 (fr)
AU (2) AU590029B2 (fr)
CA (1) CA1329157C (fr)
CZ (1) CZ280727B6 (fr)
DD (1) DD260517A5 (fr)
DE (3) DE3537708A1 (fr)
DK (2) DK175091B1 (fr)
ES (2) ES2061434T3 (fr)
FI (2) FI94050C (fr)
GR (2) GR920300062T1 (fr)
HK (2) HK153596A (fr)
HR (1) HRP921075B1 (fr)
HU (2) HUT43643A (fr)
IE (1) IE62634B1 (fr)
IL (1) IL80325A (fr)
PT (1) PT83609B (fr)
SI (1) SI8611796B (fr)
SK (1) SK752686A3 (fr)
SU (1) SU1607689A3 (fr)
UA (1) UA6023A1 (fr)
WO (1) WO1987002673A2 (fr)
YU (1) YU47185B (fr)
ZA (1) ZA868012B (fr)

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US4766205A (en) * 1985-11-13 1988-08-23 Beatrice Companies, Inc. Method for isolation of recombinant polypeptides in biologically active forms
JP2581668B2 (ja) * 1985-11-27 1997-02-12 三井東圧化学株式会社 ヒト正常細胞由来のヒト組織プラスミノ−ゲン活性化因子をコ−ドする新しいｄｎａ配列とそれを含むベクタ−及び細胞
US4777043A (en) * 1985-12-17 1988-10-11 Genentech, Inc. Stabilized human tissue plasminogen activator compositions
WO1988008428A1 (fr) * 1987-04-28 1988-11-03 Amgen Inc. Procede de purification d'un facteur de stimulation de colonies de granulocytes/macrophages
DE3722082A1 (de) * 1987-07-03 1989-01-12 Behringwerke Ag Verfahren zur bestimmung der aktivitaet von serinproteasen oder serinproteaseinhibitoren
CA1340586C (fr) * 1988-09-23 1999-06-08 Cetus Corporation Methode pour recuperer l'interferon beta produit par voie microbienne
DE3832898A1 (de) * 1988-09-28 1990-04-12 Boehringer Mannheim Gmbh Praeparat von in prokaryonten exprimiertem plasminogenaktivator
DE3835350A1 (de) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
DE3903581A1 (de) * 1989-02-07 1990-08-16 Boehringer Mannheim Gmbh Gewebs-plasminogenaktivator-derivat
DE3942143A1 (de) * 1989-12-20 1991-06-27 Boehringer Mannheim Gmbh T-pa pro stabilisierung
DE69126434D1 (de) * 1990-08-20 1997-07-10 Novo Nordisk As Prozess für die Herstellung von biologisch aktivem IGF-1 durch Verwendung von amino-terminal verlängertem IGF-1
WO1992004382A1 (fr) * 1990-09-05 1992-03-19 Bunge (Australia) Pty. Ltd. Solubilisation de proteines sous des formes actives
DE4037196A1 (de) * 1990-11-22 1992-05-27 Boehringer Mannheim Gmbh Verfahren zur reaktivierung von denaturiertem protein
DE4113750A1 (de) 1991-04-26 1992-10-29 Boehringer Mannheim Gmbh Verbesserung der renaturierung bei der sekretion von disulfidverbrueckten proteinen
DE4139000A1 (de) * 1991-11-27 1993-06-03 Boehringer Mannheim Gmbh Verfahren zur gentechnologischen herstellung von biologisch aktivem ss-ngf
US5212091A (en) * 1992-03-02 1993-05-18 Monsanto Company Method of producing tissue factor pathway inhibitor
EP0586667A1 (fr) * 1992-03-24 1994-03-16 Synergen, Inc. Repliement et purification de facteurs de croissance i ressemblant a l'insuline
DK0600372T3 (da) 1992-12-02 1997-08-11 Hoechst Ag Fremgangsmåde til fremstilling af proinsulin med korrekt forbundne cystinbroer.
DE4405179A1 (de) * 1994-02-18 1995-08-24 Hoechst Ag Verfahren zur Gewinnung von Insulin mit korrekt verbundenen Cystinbrücken
FR2729972B1 (fr) * 1995-01-31 1997-04-18 Sanofi Sa Procede d'extraction de proteines periplasmiques de microorganismes procaryotes en presence d'arginine
US5714371A (en) * 1995-05-12 1998-02-03 Schering Corporation Method for refolding insoluble aggregates of hepatitis C virus protease
US5728804A (en) * 1995-06-02 1998-03-17 Research Corporation Technologies, Inc. Use of cyclodextrins for protein renaturation
JP3288720B2 (ja) * 1996-06-11 2002-06-04 ロシュ ダイアグノスティクス ゲゼルシャフト ミット ベシュレンクテル ハフツング 変性タンパク質の活性化方法
US7153943B2 (en) 1997-07-14 2006-12-26 Bolder Biotechnology, Inc. Derivatives of growth hormone and related proteins, and methods of use thereof
US6653098B1 (en) 1998-02-23 2003-11-25 G. D. Searle & Co. Method of producing mouse and human endostatin
DE19850429A1 (de) * 1998-10-27 2000-05-04 Andre Schrattenholz Fragmente
EP1048732A1 (fr) * 1999-04-26 2000-11-02 F. Hoffmann-La Roche Ag Procédé de préparation des protéine secretées sous une forme pliée naturelle
EP1077263A1 (fr) * 1999-07-29 2001-02-21 F.Hoffmann-La Roche Ag Procédé de préparation des protéines secrétées sous une forme pliée naturelle par co-sécrétion de chaperones
AU2001274853B2 (en) 2000-05-16 2007-03-22 Bolder Biotechnology, Inc. Methods for refolding proteins containing free cysteine residues
DE10105912A1 (de) * 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Rekombinante Proteinase K
DE10105911A1 (de) 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Expression der rekombinanten Proteinase K aus Tritirachium album in Hefe
DE102005033250A1 (de) 2005-07-15 2007-01-18 Bioceuticals Arzneimittel Ag Verfahren zur Reinigung von G-CSF
DE102006009437A1 (de) 2006-03-01 2007-09-13 Bioceuticals Arzneimittel Ag G-CSF-Flüssigformulierung
KR101520105B1 (ko) 2006-07-14 2015-05-21 제넨테크, 인크. 재조합 단백질의 리폴딩
EP2102355B1 (fr) 2006-12-14 2016-03-02 Bolder Biotechnology, Inc. Protéines et peptides à action prolongée et procédés de production et d'utilisation de ces derniers
CN102892780B (zh) 2010-03-17 2015-07-29 通益制药有限公司 获得生物活性的重组人g-csf的方法
WO2012054679A1 (fr) * 2010-10-20 2012-04-26 Medimmune, Llc Méthodes de traitement de corps d'inclusion
HUP1200171A1 (hu) 2012-03-19 2013-09-30 Richter Gedeon Nyrt Módszerek polipeptidek elõállítására
HUP1200172A2 (en) 2012-03-19 2013-10-28 Richter Gedeon Nyrt Methods for refolding g-csf from inclusion bodies
CN103852527B (zh) * 2012-12-05 2015-05-13 中国科学院大连化学物理研究所 一种高通量蛋白质样品预处理装置
US10457716B2 (en) 2014-08-06 2019-10-29 University Of Notre Dame Du Lac Protein folding and methods of using same
CA3048735A1 (fr) 2016-12-30 2018-07-05 Biogend Therapeutics Co., Ltd. Polypeptides recombinants, compositions et procedes associes
FI4263568T3 (fi) 2020-12-18 2025-09-25 Richter Gedeon Nyrt Menetelmät uudelleen laskostuneen fc-peptidifuusioproteiinin puhdistamiseksi

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US4468633A (en) 1982-04-28 1984-08-28 The Bendix Corporation Adjustable microwave power combiner for a plurality of coaxially mounted impatt diodes
IL68561A (en) 1982-05-05 1991-01-31 Genentech Inc Preparation of polypeptide with human tissue plasminogen activator function,processes for making it,and dna and transformed cell intermediates thereof
US4432895A (en) * 1982-11-24 1984-02-21 Hoffmann-La Roche Inc. Monomeric interferons
GR79124B (fr) * 1982-12-22 1984-10-02 Genentech Inc
WO1984003711A1 (fr) * 1983-03-25 1984-09-27 Celltech Ltd Procede de production d'une proteine
US4530787A (en) * 1984-03-28 1985-07-23 Cetus Corporation Controlled oxidation of microbially produced cysteine-containing proteins
US4748234A (en) * 1985-06-26 1988-05-31 Cetus Corporation Process for recovering refractile bodies containing heterologous proteins from microbial hosts
US4766205A (en) * 1985-11-13 1988-08-23 Beatrice Companies, Inc. Method for isolation of recombinant polypeptides in biologically active forms
FR2596360B1 (fr) * 1986-04-01 1989-02-17 Sotralentz Sa Conteneur sur palette avec dispositif de protection en treillis plie et renforce
JPH0651119A (ja) * 1992-07-28 1994-02-25 Sekisui Chem Co Ltd 位相差板の製造方法

Also Published As

Publication number Publication date
EP0219874B1 (fr) 1993-12-15
ES2020498A4 (es) 1991-08-16
JPH0824594B2 (ja) 1996-03-13
JPH04218387A (ja) 1992-08-07
HU204855B (en) 1992-02-28
SU1607689A3 (ru) 1990-11-15
HRP921075B1 (en) 1999-02-28
DK200001897A (da) 2000-12-18
FI94050B (fi) 1995-03-31
GR3018410T3 (en) 1996-03-31
EP0393725A1 (fr) 1990-10-24
PT83609B (pt) 1988-10-14
ZA868012B (en) 1987-06-24
IE62634B1 (en) 1995-02-22
IE862683L (en) 1987-04-23
GR920300062T1 (en) 1992-08-31
HK153496A (en) 1996-08-16
ATE98648T1 (de) 1994-01-15
FI872753L (fi) 1987-06-22
DE3650449D1 (de) 1996-01-25
DK320387D0 (da) 1987-06-23
EP0253823A1 (fr) 1988-01-27
KR870700601A (ko) 1987-12-30
EP0219874A3 (en) 1988-02-10
ES2061434T3 (es) 1994-12-16
FI872753A0 (fi) 1987-06-22
JPH0728745B2 (ja) 1995-04-05
DD260517A5 (de) 1988-09-28
ATE131489T1 (de) 1995-12-15
DK175091B1 (da) 2004-05-24
FI933868A0 (fi) 1993-09-03
YU179686A (en) 1988-06-30
AU6599386A (en) 1987-05-19
CZ752686A3 (en) 1996-01-17
DE3537708C2 (fr) 1993-07-08
AU607083B2 (en) 1991-02-21
IL80325A0 (en) 1987-01-30
EP0219874A2 (fr) 1987-04-29
HK153596A (en) 1996-08-16
FI95578B (fi) 1995-11-15
FI95578C (fi) 1996-02-26
DE3689404D1 (de) 1994-01-27
CA1329157C (fr) 1994-05-03
YU47185B (sh) 1995-01-31
DK175109B1 (da) 2004-06-07
CZ280727B6 (cs) 1996-04-17
EP0393725B1 (fr) 1995-12-13
KR900009139B1 (ko) 1990-12-22
DE3537708A1 (de) 1987-04-23
PT83609A (de) 1986-11-01
SK278317B6 (en) 1996-10-02
WO1987002673A3 (fr) 1987-10-22
AU590029B2 (en) 1989-10-26
SI8611796A (sl) 1996-10-31
SI8611796B (sl) 1998-06-30
UA6023A1 (uk) 1994-12-29
DK320387A (da) 1987-06-23
ES2020498T3 (es) 1996-04-01
HRP921075A2 (en) 1995-06-30
JPS62502895A (ja) 1987-11-19
FI933868L (fi) 1993-09-03
AU4132189A (en) 1990-01-04
IL80325A (en) 1992-06-21
WO1987002673A2 (fr) 1987-05-07
FI94050C (fi) 1995-07-10
HUT43643A (en) 1987-11-30

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