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SI8611796B - Postopek za aktiviranje gentehnološko pripravljenih, heterolognih, eukariontskih proteinov, ki imajo disulfidne mostove, po ekspresiji v prokariontih - Google Patents

Postopek za aktiviranje gentehnološko pripravljenih, heterolognih, eukariontskih proteinov, ki imajo disulfidne mostove, po ekspresiji v prokariontih Download PDF

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Publication number
SI8611796B
SI8611796B SI8611796A SI8611796A SI8611796B SI 8611796 B SI8611796 B SI 8611796B SI 8611796 A SI8611796 A SI 8611796A SI 8611796 A SI8611796 A SI 8611796A SI 8611796 B SI8611796 B SI 8611796B
Authority
SI
Slovenia
Prior art keywords
process according
carried out
concentration
denaturing
mol
Prior art date
Application number
SI8611796A
Other languages
English (en)
Slovenian (sl)
Other versions
SI8611796A (sl
Inventor
Stephen Fischer
Ralf Mattes
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Publication of SI8611796A publication Critical patent/SI8611796A/sl
Publication of SI8611796B publication Critical patent/SI8611796B/sl

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Lubricants (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
SI8611796A 1985-10-23 1986-10-21 Postopek za aktiviranje gentehnološko pripravljenih, heterolognih, eukariontskih proteinov, ki imajo disulfidne mostove, po ekspresiji v prokariontih SI8611796B (sl)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19853537708 DE3537708A1 (de) 1985-10-23 1985-10-23 Verfahren zur aktivierung von t-pa nach expression in prokaryonten
YU179686A YU47185B (sh) 1985-10-23 1986-10-21 Postupak za aktiviranje heterolognih eukariotskih proteina, pripremljenih gen-tehnologijom koji imaju disulfidne mostove polse ekspresije u prokariotima

Publications (2)

Publication Number Publication Date
SI8611796A SI8611796A (sl) 1996-10-31
SI8611796B true SI8611796B (sl) 1998-06-30

Family

ID=6284269

Family Applications (1)

Application Number Title Priority Date Filing Date
SI8611796A SI8611796B (sl) 1985-10-23 1986-10-21 Postopek za aktiviranje gentehnološko pripravljenih, heterolognih, eukariontskih proteinov, ki imajo disulfidne mostove, po ekspresiji v prokariontih

Country Status (26)

Country Link
EP (3) EP0219874B1 (fr)
JP (2) JPH0728745B2 (fr)
KR (1) KR900009139B1 (fr)
AT (2) ATE131489T1 (fr)
AU (2) AU590029B2 (fr)
CA (1) CA1329157C (fr)
CZ (1) CZ280727B6 (fr)
DD (1) DD260517A5 (fr)
DE (3) DE3537708A1 (fr)
DK (2) DK175091B1 (fr)
ES (2) ES2061434T3 (fr)
FI (2) FI94050C (fr)
GR (2) GR920300062T1 (fr)
HK (2) HK153596A (fr)
HR (1) HRP921075B1 (fr)
HU (2) HUT43643A (fr)
IE (1) IE62634B1 (fr)
IL (1) IL80325A (fr)
PT (1) PT83609B (fr)
SI (1) SI8611796B (fr)
SK (1) SK278317B6 (fr)
SU (1) SU1607689A3 (fr)
UA (1) UA6023A1 (fr)
WO (1) WO1987002673A2 (fr)
YU (1) YU47185B (fr)
ZA (1) ZA868012B (fr)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4766205A (en) * 1985-11-13 1988-08-23 Beatrice Companies, Inc. Method for isolation of recombinant polypeptides in biologically active forms
JP2581668B2 (ja) * 1985-11-27 1997-02-12 三井東圧化学株式会社 ヒト正常細胞由来のヒト組織プラスミノ−ゲン活性化因子をコ−ドする新しいｄｎａ配列とそれを含むベクタ−及び細胞
US4777043A (en) * 1985-12-17 1988-10-11 Genentech, Inc. Stabilized human tissue plasminogen activator compositions
WO1988008428A1 (fr) * 1987-04-28 1988-11-03 Amgen Inc. Procede de purification d'un facteur de stimulation de colonies de granulocytes/macrophages
DE3722082A1 (de) * 1987-07-03 1989-01-12 Behringwerke Ag Verfahren zur bestimmung der aktivitaet von serinproteasen oder serinproteaseinhibitoren
CA1340586C (fr) * 1988-09-23 1999-06-08 Cetus Corporation Methode pour recuperer l'interferon beta produit par voie microbienne
DE3832898A1 (de) * 1988-09-28 1990-04-12 Boehringer Mannheim Gmbh Praeparat von in prokaryonten exprimiertem plasminogenaktivator
DE3835350A1 (de) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
DE3903581A1 (de) * 1989-02-07 1990-08-16 Boehringer Mannheim Gmbh Gewebs-plasminogenaktivator-derivat
DE3942143A1 (de) * 1989-12-20 1991-06-27 Boehringer Mannheim Gmbh T-pa pro stabilisierung
WO1992003477A1 (fr) * 1990-08-20 1992-03-05 Novo Nordisk A/S Compose biologiquement actif, procede de preparation et utilisation de celui-ci
ES2119779T3 (es) * 1990-09-05 1998-10-16 Southern Cross Biotech Pty Ltd Solubilizacion de proteinas en formas activas.
DE4037196A1 (de) * 1990-11-22 1992-05-27 Boehringer Mannheim Gmbh Verfahren zur reaktivierung von denaturiertem protein
DE4113750A1 (de) * 1991-04-26 1992-10-29 Boehringer Mannheim Gmbh Verbesserung der renaturierung bei der sekretion von disulfidverbrueckten proteinen
DE4139000A1 (de) 1991-11-27 1993-06-03 Boehringer Mannheim Gmbh Verfahren zur gentechnologischen herstellung von biologisch aktivem ss-ngf
US5212091A (en) * 1992-03-02 1993-05-18 Monsanto Company Method of producing tissue factor pathway inhibitor
CA2109820A1 (fr) * 1992-03-24 1993-09-30 George N. Cox Repliement et purification du facteur de croissance apparente a l'insuline i
ES2097426T3 (es) 1992-12-02 1997-04-01 Hoechst Ag Procedimiento para la obtencion de proinsulina con puentes de cistina correctamente unidos.
DE4405179A1 (de) * 1994-02-18 1995-08-24 Hoechst Ag Verfahren zur Gewinnung von Insulin mit korrekt verbundenen Cystinbrücken
FR2729972B1 (fr) * 1995-01-31 1997-04-18 Sanofi Sa Procede d'extraction de proteines periplasmiques de microorganismes procaryotes en presence d'arginine
US5714371A (en) * 1995-05-12 1998-02-03 Schering Corporation Method for refolding insoluble aggregates of hepatitis C virus protease
US5728804A (en) * 1995-06-02 1998-03-17 Research Corporation Technologies, Inc. Use of cyclodextrins for protein renaturation
ATE260972T1 (de) * 1996-06-11 2004-03-15 Roche Diagnostics Gmbh Verfahren zur aktivierung von denaturiertem protein
US7306931B2 (en) 2000-05-16 2007-12-11 Bolder Biotechnology, Inc. Method for refolding proteins containing free cysteine residues
US7153943B2 (en) 1997-07-14 2006-12-26 Bolder Biotechnology, Inc. Derivatives of growth hormone and related proteins, and methods of use thereof
US6653098B1 (en) * 1998-02-23 2003-11-25 G. D. Searle & Co. Method of producing mouse and human endostatin
DE19850429A1 (de) * 1998-10-27 2000-05-04 Andre Schrattenholz Fragmente
EP1048732A1 (fr) 1999-04-26 2000-11-02 F. Hoffmann-La Roche Ag Procédé de préparation des protéine secretées sous une forme pliée naturelle
EP1077263A1 (fr) * 1999-07-29 2001-02-21 F.Hoffmann-La Roche Ag Procédé de préparation des protéines secrétées sous une forme pliée naturelle par co-sécrétion de chaperones
DE10105912A1 (de) * 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Rekombinante Proteinase K
DE10105911A1 (de) 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Expression der rekombinanten Proteinase K aus Tritirachium album in Hefe
DE102005033250A1 (de) 2005-07-15 2007-01-18 Bioceuticals Arzneimittel Ag Verfahren zur Reinigung von G-CSF
DE202006020194U1 (de) 2006-03-01 2007-12-06 Bioceuticals Arzneimittel Ag G-CSF-Flüssigformulierung
CN101506222B (zh) 2006-07-14 2013-10-02 健泰科生物技术公司 重组蛋白的重折叠
EP2102355B1 (fr) 2006-12-14 2016-03-02 Bolder Biotechnology, Inc. Protéines et peptides à action prolongée et procédés de production et d'utilisation de ces derniers
MX2012010661A (es) 2010-03-17 2013-03-25 Biogenerix Gmbh Metodo para obtener factor estimulante de colonias de granulocitos (g-csf) humano recombinante biologicamente activo.
KR20130140010A (ko) * 2010-10-20 2013-12-23 메디뮨 엘엘씨 봉입체의 가공처리 방법
HUP1200172A2 (en) 2012-03-19 2013-10-28 Richter Gedeon Nyrt Methods for refolding g-csf from inclusion bodies
HUP1200171A1 (hu) 2012-03-19 2013-09-30 Richter Gedeon Nyrt Módszerek polipeptidek elõállítására
CN103852527B (zh) * 2012-12-05 2015-05-13 中国科学院大连化学物理研究所 一种高通量蛋白质样品预处理装置
US10457716B2 (en) 2014-08-06 2019-10-29 University Of Notre Dame Du Lac Protein folding and methods of using same
EP3562839A4 (fr) 2016-12-30 2020-09-02 Biogend Therapeutics Co., Ltd. Polypeptides recombinants, compositions et procédés associés
EP4263568B1 (fr) 2020-12-18 2025-06-18 Richter Gedeon Nyrt. Procédés de purification de protéine de fusion avec un fc-peptide repliée

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4468633A (en) 1982-04-28 1984-08-28 The Bendix Corporation Adjustable microwave power combiner for a plurality of coaxially mounted impatt diodes
IL68561A (en) 1982-05-05 1991-01-31 Genentech Inc Preparation of polypeptide with human tissue plasminogen activator function,processes for making it,and dna and transformed cell intermediates thereof
US4432895A (en) * 1982-11-24 1984-02-21 Hoffmann-La Roche Inc. Monomeric interferons
GR79124B (fr) * 1982-12-22 1984-10-02 Genentech Inc
GB2138004B (en) * 1983-03-25 1987-05-13 Celltech Ltd A process for the production of a protein
US4530787A (en) * 1984-03-28 1985-07-23 Cetus Corporation Controlled oxidation of microbially produced cysteine-containing proteins
US4748234A (en) * 1985-06-26 1988-05-31 Cetus Corporation Process for recovering refractile bodies containing heterologous proteins from microbial hosts
US4766205A (en) * 1985-11-13 1988-08-23 Beatrice Companies, Inc. Method for isolation of recombinant polypeptides in biologically active forms
FR2596360B1 (fr) * 1986-04-01 1989-02-17 Sotralentz Sa Conteneur sur palette avec dispositif de protection en treillis plie et renforce
JPH0651119A (ja) * 1992-07-28 1994-02-25 Sekisui Chem Co Ltd 位相差板の製造方法

Also Published As

Publication number Publication date
AU6599386A (en) 1987-05-19
ES2020498A4 (es) 1991-08-16
DD260517A5 (de) 1988-09-28
KR900009139B1 (ko) 1990-12-22
PT83609B (pt) 1988-10-14
HUT43643A (en) 1987-11-30
IE862683L (en) 1987-04-23
SK752686A3 (en) 1996-10-02
FI933868L (fi) 1993-09-03
FI95578B (fi) 1995-11-15
FI95578C (fi) 1996-02-26
AU590029B2 (en) 1989-10-26
JPS62502895A (ja) 1987-11-19
IL80325A0 (en) 1987-01-30
EP0393725B1 (fr) 1995-12-13
DK320387A (da) 1987-06-23
ES2020498T3 (es) 1996-04-01
DK175109B1 (da) 2004-06-07
HRP921075A2 (en) 1995-06-30
HRP921075B1 (en) 1999-02-28
DK175091B1 (da) 2004-05-24
JPH0824594B2 (ja) 1996-03-13
CZ752686A3 (en) 1996-01-17
DE3537708C2 (fr) 1993-07-08
JPH04218387A (ja) 1992-08-07
KR870700601A (ko) 1987-12-30
IL80325A (en) 1992-06-21
ZA868012B (en) 1987-06-24
GR3018410T3 (en) 1996-03-31
EP0253823A1 (fr) 1988-01-27
FI933868A0 (fi) 1993-09-03
SI8611796A (sl) 1996-10-31
DK320387D0 (da) 1987-06-23
FI872753A0 (fi) 1987-06-22
DK200001897A (da) 2000-12-18
PT83609A (de) 1986-11-01
DE3650449D1 (de) 1996-01-25
FI94050B (fi) 1995-03-31
UA6023A1 (uk) 1994-12-29
WO1987002673A2 (fr) 1987-05-07
EP0393725A1 (fr) 1990-10-24
HU204855B (en) 1992-02-28
ES2061434T3 (es) 1994-12-16
DE3537708A1 (de) 1987-04-23
CZ280727B6 (cs) 1996-04-17
AU607083B2 (en) 1991-02-21
HK153496A (en) 1996-08-16
JPH0728745B2 (ja) 1995-04-05
EP0219874B1 (fr) 1993-12-15
EP0219874A3 (en) 1988-02-10
CA1329157C (fr) 1994-05-03
EP0219874A2 (fr) 1987-04-29
ATE131489T1 (de) 1995-12-15
FI94050C (fi) 1995-07-10
YU179686A (en) 1988-06-30
DE3689404D1 (de) 1994-01-27
ATE98648T1 (de) 1994-01-15
YU47185B (sh) 1995-01-31
WO1987002673A3 (fr) 1987-10-22
SK278317B6 (en) 1996-10-02
AU4132189A (en) 1990-01-04
IE62634B1 (en) 1995-02-22
HK153596A (en) 1996-08-16
SU1607689A3 (ru) 1990-11-15
FI872753L (fi) 1987-06-22
GR920300062T1 (en) 1992-08-31

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