GR3018410T3 - Process for activating genetically-engineered, heterologous eucaryotic proteins containing disulphide bridges after their expression in procaryotes - Google Patents
Process for activating genetically-engineered, heterologous eucaryotic proteins containing disulphide bridges after their expression in procaryotesInfo
- Publication number
- GR3018410T3 GR3018410T3 GR950403376T GR950403376T GR3018410T3 GR 3018410 T3 GR3018410 T3 GR 3018410T3 GR 950403376 T GR950403376 T GR 950403376T GR 950403376 T GR950403376 T GR 950403376T GR 3018410 T3 GR3018410 T3 GR 3018410T3
- Authority
- GR
- Greece
- Prior art keywords
- pref
- expression
- procaryotes
- engineered
- esp
- Prior art date
Links
- 230000003213 activating effect Effects 0.000 title abstract 2
- 102000004169 proteins and genes Human genes 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 102000003978 Tissue Plasminogen Activator Human genes 0.000 abstract 3
- 108090000373 Tissue Plasminogen Activator Proteins 0.000 abstract 3
- 229960000187 tissue plasminogen activator Drugs 0.000 abstract 3
- 239000003795 chemical substances by application Substances 0.000 abstract 2
- 239000004475 Arginine Substances 0.000 abstract 1
- 241000588724 Escherichia coli Species 0.000 abstract 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 abstract 1
- 241000589776 Pseudomonas putida Species 0.000 abstract 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 abstract 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 abstract 1
- 239000004202 carbamide Substances 0.000 abstract 1
- 230000006037 cell lysis Effects 0.000 abstract 1
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 abstract 1
- YPZRWBKMTBYPTK-BJDJZHNGSA-N glutathione disulfide Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@H](C(=O)NCC(O)=O)CSSC[C@@H](C(=O)NCC(O)=O)NC(=O)CC[C@H](N)C(O)=O YPZRWBKMTBYPTK-BJDJZHNGSA-N 0.000 abstract 1
- 229960000789 guanidine hydrochloride Drugs 0.000 abstract 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 abstract 1
- 210000001236 prokaryotic cell Anatomy 0.000 abstract 1
- 230000007420 reactivation Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/565—IFN-beta
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Mechanical Treatment Of Semiconductor (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Lubricants (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19853537708 DE3537708A1 (de) | 1985-10-23 | 1985-10-23 | Verfahren zur aktivierung von t-pa nach expression in prokaryonten |
| EP86114731A EP0219874B1 (de) | 1985-10-23 | 1986-10-23 | Verfahren zur Aktivierung von gentechnologisch hergestellten, heterologen, Disulfidbrücken aufweisenden eukaryontischen Proteinen nach Expression in Prokaryonten |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GR3018410T3 true GR3018410T3 (en) | 1996-03-31 |
Family
ID=6284269
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GR92300062T GR920300062T1 (en) | 1985-10-23 | 1992-08-31 | Process for activating recombinant heterologous eucaryotic disulfide-bridged proteins after expression by procaryotes |
| GR950403376T GR3018410T3 (en) | 1985-10-23 | 1995-12-14 | Process for activating genetically-engineered, heterologous eucaryotic proteins containing disulphide bridges after their expression in procaryotes |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GR92300062T GR920300062T1 (en) | 1985-10-23 | 1992-08-31 | Process for activating recombinant heterologous eucaryotic disulfide-bridged proteins after expression by procaryotes |
Country Status (26)
| Country | Link |
|---|---|
| EP (3) | EP0219874B1 (el) |
| JP (2) | JPH0728745B2 (el) |
| KR (1) | KR900009139B1 (el) |
| AT (2) | ATE131489T1 (el) |
| AU (2) | AU590029B2 (el) |
| CA (1) | CA1329157C (el) |
| CZ (1) | CZ280727B6 (el) |
| DD (1) | DD260517A5 (el) |
| DE (3) | DE3537708A1 (el) |
| DK (2) | DK175091B1 (el) |
| ES (2) | ES2020498T3 (el) |
| FI (2) | FI94050C (el) |
| GR (2) | GR920300062T1 (el) |
| HK (2) | HK153496A (el) |
| HR (1) | HRP921075B1 (el) |
| HU (2) | HUT43643A (el) |
| IE (1) | IE62634B1 (el) |
| IL (1) | IL80325A (el) |
| PT (1) | PT83609B (el) |
| SI (1) | SI8611796B (el) |
| SK (1) | SK752686A3 (el) |
| SU (1) | SU1607689A3 (el) |
| UA (1) | UA6023A1 (el) |
| WO (1) | WO1987002673A2 (el) |
| YU (1) | YU47185B (el) |
| ZA (1) | ZA868012B (el) |
Families Citing this family (43)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4766205A (en) * | 1985-11-13 | 1988-08-23 | Beatrice Companies, Inc. | Method for isolation of recombinant polypeptides in biologically active forms |
| JP2581668B2 (ja) * | 1985-11-27 | 1997-02-12 | 三井東圧化学株式会社 | ヒト正常細胞由来のヒト組織プラスミノ−ゲン活性化因子をコ−ドする新しいｄｎａ配列とそれを含むベクタ−及び細胞 |
| US4777043A (en) * | 1985-12-17 | 1988-10-11 | Genentech, Inc. | Stabilized human tissue plasminogen activator compositions |
| WO1988008428A1 (en) * | 1987-04-28 | 1988-11-03 | Amgen Inc. | Method for purifying granulocyte/macrophage colony stimulating factor |
| DE3722082A1 (de) * | 1987-07-03 | 1989-01-12 | Behringwerke Ag | Verfahren zur bestimmung der aktivitaet von serinproteasen oder serinproteaseinhibitoren |
| CA1340586C (en) * | 1988-09-23 | 1999-06-08 | Cetus Corporation | Process for recovering microbially produced interferon-beta |
| DE3832898A1 (de) * | 1988-09-28 | 1990-04-12 | Boehringer Mannheim Gmbh | Praeparat von in prokaryonten exprimiertem plasminogenaktivator |
| DE3835350A1 (de) * | 1988-10-17 | 1990-04-19 | Boehringer Mannheim Gmbh | Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern |
| DE3903581A1 (de) * | 1989-02-07 | 1990-08-16 | Boehringer Mannheim Gmbh | Gewebs-plasminogenaktivator-derivat |
| DE3942143A1 (de) * | 1989-12-20 | 1991-06-27 | Boehringer Mannheim Gmbh | T-pa pro stabilisierung |
| JPH06500084A (ja) * | 1990-08-20 | 1994-01-06 | ノボ ノルディスク アクティーゼルスカブ | 生物学的に活性な化合物、その製造方法及びその利用 |
| EP0547102B1 (en) * | 1990-09-05 | 1998-07-08 | Southern Cross Biotech Pty.Ltd. | Solubilization of proteins in active forms |
| DE4037196A1 (de) * | 1990-11-22 | 1992-05-27 | Boehringer Mannheim Gmbh | Verfahren zur reaktivierung von denaturiertem protein |
| DE4113750A1 (de) | 1991-04-26 | 1992-10-29 | Boehringer Mannheim Gmbh | Verbesserung der renaturierung bei der sekretion von disulfidverbrueckten proteinen |
| DE4139000A1 (de) | 1991-11-27 | 1993-06-03 | Boehringer Mannheim Gmbh | Verfahren zur gentechnologischen herstellung von biologisch aktivem ss-ngf |
| US5212091A (en) * | 1992-03-02 | 1993-05-18 | Monsanto Company | Method of producing tissue factor pathway inhibitor |
| WO1993019084A1 (en) * | 1992-03-24 | 1993-09-30 | Synergen, Inc. | Refolding and purification of insulin-like growth factor i |
| SG46612A1 (en) | 1992-12-02 | 1998-02-20 | Hoechst Ag | Process for obtaining proinsulin in processing correctly linked cystine bridges |
| DE4405179A1 (de) * | 1994-02-18 | 1995-08-24 | Hoechst Ag | Verfahren zur Gewinnung von Insulin mit korrekt verbundenen Cystinbrücken |
| FR2729972B1 (fr) * | 1995-01-31 | 1997-04-18 | Sanofi Sa | Procede d'extraction de proteines periplasmiques de microorganismes procaryotes en presence d'arginine |
| US5714371A (en) * | 1995-05-12 | 1998-02-03 | Schering Corporation | Method for refolding insoluble aggregates of hepatitis C virus protease |
| US5728804A (en) * | 1995-06-02 | 1998-03-17 | Research Corporation Technologies, Inc. | Use of cyclodextrins for protein renaturation |
| US6342585B1 (en) * | 1996-06-11 | 2002-01-29 | Roche Diagnostics Gmbh | Method of activating denatured protein |
| US7153943B2 (en) | 1997-07-14 | 2006-12-26 | Bolder Biotechnology, Inc. | Derivatives of growth hormone and related proteins, and methods of use thereof |
| US6653098B1 (en) * | 1998-02-23 | 2003-11-25 | G. D. Searle & Co. | Method of producing mouse and human endostatin |
| DE19850429A1 (de) * | 1998-10-27 | 2000-05-04 | Andre Schrattenholz | Fragmente |
| EP1048732A1 (de) | 1999-04-26 | 2000-11-02 | F. Hoffmann-La Roche Ag | Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen |
| EP1077263A1 (de) * | 1999-07-29 | 2001-02-21 | F.Hoffmann-La Roche Ag | Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen durch Co-Sekretion von Chaperonen |
| NZ522847A (en) | 2000-05-16 | 2004-11-26 | Bolder Biotechnology Inc | Methods for refolding proteins containing free cysteine residues |
| DE10105912A1 (de) * | 2001-02-09 | 2002-08-14 | Roche Diagnostics Gmbh | Rekombinante Proteinase K |
| DE10105911A1 (de) | 2001-02-09 | 2002-08-14 | Roche Diagnostics Gmbh | Expression der rekombinanten Proteinase K aus Tritirachium album in Hefe |
| DE102005033250A1 (de) | 2005-07-15 | 2007-01-18 | Bioceuticals Arzneimittel Ag | Verfahren zur Reinigung von G-CSF |
| DE202006020194U1 (de) | 2006-03-01 | 2007-12-06 | Bioceuticals Arzneimittel Ag | G-CSF-Flüssigformulierung |
| WO2008008975A2 (en) | 2006-07-14 | 2008-01-17 | Genentech, Inc. | Refolding of recombinant proteins |
| US8617531B2 (en) | 2006-12-14 | 2013-12-31 | Bolder Biotechnology, Inc. | Methods of making proteins and peptides containing a single free cysteine |
| CN102892780B (zh) | 2010-03-17 | 2015-07-29 | 通益制药有限公司 | 获得生物活性的重组人g-csf的方法 |
| WO2012054679A1 (en) * | 2010-10-20 | 2012-04-26 | Medimmune, Llc | Methods for processing inclusion bodies |
| HUP1200171A1 (hu) | 2012-03-19 | 2013-09-30 | Richter Gedeon Nyrt | Módszerek polipeptidek elõállítására |
| HUP1200172A2 (en) | 2012-03-19 | 2013-10-28 | Richter Gedeon Nyrt | Methods for refolding g-csf from inclusion bodies |
| CN103852527B (zh) * | 2012-12-05 | 2015-05-13 | 中国科学院大连化学物理研究所 | 一种高通量蛋白质样品预处理装置 |
| US10457716B2 (en) | 2014-08-06 | 2019-10-29 | University Of Notre Dame Du Lac | Protein folding and methods of using same |
| JP6629488B2 (ja) | 2016-12-30 | 2020-01-15 | バイオジェンド セラピューティクス カンパニー リミテッド | 組換えポリペプチド、核酸分子、それらを含む組成物及びそれらの製造、使用方法 |
| AU2021399935A1 (en) | 2020-12-18 | 2023-06-29 | Richter Gedeon Nyrt. | Methods for the purification of refolded fc-peptide fusion protein |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4468633A (en) | 1982-04-28 | 1984-08-28 | The Bendix Corporation | Adjustable microwave power combiner for a plurality of coaxially mounted impatt diodes |
| IL68561A (en) | 1982-05-05 | 1991-01-31 | Genentech Inc | Preparation of polypeptide with human tissue plasminogen activator function,processes for making it,and dna and transformed cell intermediates thereof |
| US4432895A (en) * | 1982-11-24 | 1984-02-21 | Hoffmann-La Roche Inc. | Monomeric interferons |
| GR79124B (el) * | 1982-12-22 | 1984-10-02 | Genentech Inc | |
| EP0122080B1 (en) * | 1983-03-25 | 1989-09-13 | Celltech Limited | A process for the production of a protein |
| US4530787A (en) * | 1984-03-28 | 1985-07-23 | Cetus Corporation | Controlled oxidation of microbially produced cysteine-containing proteins |
| US4748234A (en) * | 1985-06-26 | 1988-05-31 | Cetus Corporation | Process for recovering refractile bodies containing heterologous proteins from microbial hosts |
| US4766205A (en) * | 1985-11-13 | 1988-08-23 | Beatrice Companies, Inc. | Method for isolation of recombinant polypeptides in biologically active forms |
| FR2596360B1 (fr) * | 1986-04-01 | 1989-02-17 | Sotralentz Sa | Conteneur sur palette avec dispositif de protection en treillis plie et renforce |
| JPH0651119A (ja) * | 1992-07-28 | 1994-02-25 | Sekisui Chem Co Ltd | 位相差板の製造方法 |
-
1985
- 1985-10-23 DE DE19853537708 patent/DE3537708A1/de active Granted
-
1986
- 1986-10-10 IE IE268386A patent/IE62634B1/en not_active IP Right Cessation
- 1986-10-15 IL IL80325A patent/IL80325A/xx not_active IP Right Cessation
- 1986-10-17 SK SK7526-86A patent/SK752686A3/sk unknown
- 1986-10-17 CZ CS867526A patent/CZ280727B6/cs not_active IP Right Cessation
- 1986-10-21 YU YU179686A patent/YU47185B/sh unknown
- 1986-10-21 SI SI8611796A patent/SI8611796B/sl unknown
- 1986-10-22 DD DD29546886A patent/DD260517A5/de not_active IP Right Cessation
- 1986-10-22 ZA ZA868012A patent/ZA868012B/xx unknown
- 1986-10-22 CA CA000521121A patent/CA1329157C/en not_active Expired - Lifetime
- 1986-10-23 EP EP86114731A patent/EP0219874B1/de not_active Expired - Lifetime
- 1986-10-23 AU AU65993/86A patent/AU590029B2/en not_active Ceased
- 1986-10-23 ES ES90109721T patent/ES2020498T3/es not_active Expired - Lifetime
- 1986-10-23 JP JP61505882A patent/JPH0728745B2/ja not_active Expired - Lifetime
- 1986-10-23 KR KR1019870700536A patent/KR900009139B1/ko not_active Expired
- 1986-10-23 HU HU865290A patent/HUT43643A/hu unknown
- 1986-10-23 WO PCT/EP1986/000610 patent/WO1987002673A2/de not_active Ceased
- 1986-10-23 DE DE3650449T patent/DE3650449D1/de not_active Expired - Lifetime
- 1986-10-23 EP EP86906320A patent/EP0253823A1/de active Pending
- 1986-10-23 EP EP90109721A patent/EP0393725B1/de not_active Expired - Lifetime
- 1986-10-23 UA UA4202987A patent/UA6023A1/uk unknown
- 1986-10-23 AT AT90109721T patent/ATE131489T1/de not_active IP Right Cessation
- 1986-10-23 PT PT83609A patent/PT83609B/pt not_active IP Right Cessation
- 1986-10-23 DE DE86114731T patent/DE3689404D1/de not_active Expired - Lifetime
- 1986-10-23 ES ES86114731T patent/ES2061434T3/es not_active Expired - Lifetime
- 1986-10-23 HU HU865290A patent/HU204855B/hu unknown
- 1986-10-23 AT AT86114731T patent/ATE98648T1/de not_active IP Right Cessation
-
1987
- 1987-06-22 FI FI872753A patent/FI94050C/fi not_active IP Right Cessation
- 1987-06-22 SU SU874202987Q patent/SU1607689A3/ru active
- 1987-06-23 DK DK198703203A patent/DK175091B1/da not_active IP Right Cessation
-
1989
- 1989-09-13 AU AU41321/89A patent/AU607083B2/en not_active Expired
-
1991
- 1991-04-12 JP JP3079762A patent/JPH0824594B2/ja not_active Expired - Lifetime
-
1992
- 1992-08-31 GR GR92300062T patent/GR920300062T1/el unknown
- 1992-10-16 HR HRP-1796/86A patent/HRP921075B1/xx not_active IP Right Cessation
-
1993
- 1993-09-03 FI FI933868A patent/FI95578C/fi not_active IP Right Cessation
-
1995
- 1995-12-14 GR GR950403376T patent/GR3018410T3/el unknown
-
1996
- 1996-08-08 HK HK153496A patent/HK153496A/xx not_active IP Right Cessation
- 1996-08-08 HK HK153596A patent/HK153596A/xx not_active IP Right Cessation
-
2000
- 2000-12-18 DK DK200001897A patent/DK175109B1/da not_active IP Right Cessation
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| GR3018410T3 (en) | Process for activating genetically-engineered, heterologous eucaryotic proteins containing disulphide bridges after their expression in procaryotes | |
| ATE80166T1 (de) | Verfahren zur isolierung rekombinanter polypeptide in biologisch aktiver form. | |
| ES469328A1 (es) | Procedimiento para el enriquecimiento de una nueva glucopre-teina. | |
| AU587423B2 (en) | Method of somatotropin solubilization and naturation | |
| Nakano et al. | Purification and characterization of a gelatinase produced by fibroblasts from human gingiva | |
| HUT40140A (en) | Process for solubilizing and restoring homatropin | |
| CHIEN | Threshold Limit Value of Subtilising |