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KR870700601A - 원핵생물내 발현후 유전공학적으로 제조된 이황화 결합을 갖는 진핵생물 단백질의 활성화 방법 - Google Patents

원핵생물내 발현후 유전공학적으로 제조된 이황화 결합을 갖는 진핵생물 단백질의 활성화 방법

Info

Publication number
KR870700601A
KR870700601A KR870700536A KR870700536A KR870700601A KR 870700601 A KR870700601 A KR 870700601A KR 870700536 A KR870700536 A KR 870700536A KR 870700536 A KR870700536 A KR 870700536A KR 870700601 A KR870700601 A KR 870700601A
Authority
KR
South Korea
Prior art keywords
pref
esp
genetically engineered
disulfide bonds
denaturing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
KR870700536A
Other languages
English (en)
Other versions
KR900009139B1 (ko
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed filed Critical
Publication of KR870700601A publication Critical patent/KR870700601A/ko
Application granted granted Critical
Publication of KR900009139B1 publication Critical patent/KR900009139B1/ko
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)
  • Lubricants (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
KR1019870700536A 1985-10-23 1986-10-23 유전공학적으로 제조된, 다른 종류의, 이황화 결합을 갖는 진핵생물 단백질을 원핵생물에 있어서의 발현 후에 활성화시키는 방법 Expired KR900009139B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19853537708 DE3537708A1 (de) 1985-10-23 1985-10-23 Verfahren zur aktivierung von t-pa nach expression in prokaryonten
DEP3537708.9 1985-10-23
PCT/EP1986/000610 WO1987002673A2 (en) 1985-10-23 1986-10-23 Process for activating heterologous, eucaryotic proteins genetically engineered and presenting disulphide bridges after their expression in procaryotic cells

Publications (2)

Publication Number Publication Date
KR870700601A true KR870700601A (ko) 1987-12-30
KR900009139B1 KR900009139B1 (ko) 1990-12-22

Family

ID=6284269

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019870700536A Expired KR900009139B1 (ko) 1985-10-23 1986-10-23 유전공학적으로 제조된, 다른 종류의, 이황화 결합을 갖는 진핵생물 단백질을 원핵생물에 있어서의 발현 후에 활성화시키는 방법

Country Status (26)

Country Link
EP (3) EP0219874B1 (ko)
JP (2) JPH0728745B2 (ko)
KR (1) KR900009139B1 (ko)
AT (2) ATE131489T1 (ko)
AU (2) AU590029B2 (ko)
CA (1) CA1329157C (ko)
CZ (1) CZ280727B6 (ko)
DD (1) DD260517A5 (ko)
DE (3) DE3537708A1 (ko)
DK (2) DK175091B1 (ko)
ES (2) ES2020498T3 (ko)
FI (2) FI94050C (ko)
GR (2) GR920300062T1 (ko)
HK (2) HK153496A (ko)
HR (1) HRP921075B1 (ko)
HU (2) HU204855B (ko)
IE (1) IE62634B1 (ko)
IL (1) IL80325A (ko)
PT (1) PT83609B (ko)
SI (1) SI8611796B (ko)
SK (1) SK752686A3 (ko)
SU (1) SU1607689A3 (ko)
UA (1) UA6023A1 (ko)
WO (1) WO1987002673A2 (ko)
YU (1) YU47185B (ko)
ZA (1) ZA868012B (ko)

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US4766205A (en) * 1985-11-13 1988-08-23 Beatrice Companies, Inc. Method for isolation of recombinant polypeptides in biologically active forms
JP2581668B2 (ja) * 1985-11-27 1997-02-12 三井東圧化学株式会社 ヒト正常細胞由来のヒト組織プラスミノ−ゲン活性化因子をコ−ドする新しいｄｎａ配列とそれを含むベクタ−及び細胞
US4777043A (en) * 1985-12-17 1988-10-11 Genentech, Inc. Stabilized human tissue plasminogen activator compositions
WO1988008428A1 (en) * 1987-04-28 1988-11-03 Amgen Inc. Method for purifying granulocyte/macrophage colony stimulating factor
DE3722082A1 (de) * 1987-07-03 1989-01-12 Behringwerke Ag Verfahren zur bestimmung der aktivitaet von serinproteasen oder serinproteaseinhibitoren
CA1340586C (en) * 1988-09-23 1999-06-08 Cetus Corporation Process for recovering microbially produced interferon-beta
DE3832898A1 (de) * 1988-09-28 1990-04-12 Boehringer Mannheim Gmbh Praeparat von in prokaryonten exprimiertem plasminogenaktivator
DE3835350A1 (de) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
DE3903581A1 (de) * 1989-02-07 1990-08-16 Boehringer Mannheim Gmbh Gewebs-plasminogenaktivator-derivat
DE3942143A1 (de) * 1989-12-20 1991-06-27 Boehringer Mannheim Gmbh T-pa pro stabilisierung
JPH06500084A (ja) * 1990-08-20 1994-01-06 ノボ　ノルディスク　アクティーゼルスカブ 生物学的に活性な化合物、その製造方法及びその利用
EP0547102B1 (en) * 1990-09-05 1998-07-08 Southern Cross Biotech Pty.Ltd. Solubilization of proteins in active forms
DE4037196A1 (de) * 1990-11-22 1992-05-27 Boehringer Mannheim Gmbh Verfahren zur reaktivierung von denaturiertem protein
DE4113750A1 (de) 1991-04-26 1992-10-29 Boehringer Mannheim Gmbh Verbesserung der renaturierung bei der sekretion von disulfidverbrueckten proteinen
DE4139000A1 (de) 1991-11-27 1993-06-03 Boehringer Mannheim Gmbh Verfahren zur gentechnologischen herstellung von biologisch aktivem ss-ngf
US5212091A (en) * 1992-03-02 1993-05-18 Monsanto Company Method of producing tissue factor pathway inhibitor
CA2109820A1 (en) * 1992-03-24 1993-09-30 George N. Cox Refolding and purification of insulin-like growth factor i
EP0600372B1 (de) 1992-12-02 1997-02-05 Hoechst Aktiengesellschaft Verfahren zur Gewinnung von Proinsulin mit korrekt verbundenen Cystinbrücken
DE4405179A1 (de) * 1994-02-18 1995-08-24 Hoechst Ag Verfahren zur Gewinnung von Insulin mit korrekt verbundenen Cystinbrücken
FR2729972B1 (fr) * 1995-01-31 1997-04-18 Sanofi Sa Procede d'extraction de proteines periplasmiques de microorganismes procaryotes en presence d'arginine
US5714371A (en) * 1995-05-12 1998-02-03 Schering Corporation Method for refolding insoluble aggregates of hepatitis C virus protease
US5728804A (en) * 1995-06-02 1998-03-17 Research Corporation Technologies, Inc. Use of cyclodextrins for protein renaturation
KR100305341B1 (ko) * 1996-06-11 2002-03-02 로셰 디아그노스틱스 게엠베하 변성된단백질의활성화방법
US7153943B2 (en) 1997-07-14 2006-12-26 Bolder Biotechnology, Inc. Derivatives of growth hormone and related proteins, and methods of use thereof
US6653098B1 (en) 1998-02-23 2003-11-25 G. D. Searle & Co. Method of producing mouse and human endostatin
DE19850429A1 (de) * 1998-10-27 2000-05-04 Andre Schrattenholz Fragmente
EP1048732A1 (de) * 1999-04-26 2000-11-02 F. Hoffmann-La Roche Ag Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen
EP1077263A1 (de) * 1999-07-29 2001-02-21 F.Hoffmann-La Roche Ag Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen durch Co-Sekretion von Chaperonen
JP4873818B2 (ja) 2000-05-16 2012-02-08 ボルダー バイオテクノロジー， インコーポレイテッド 遊離システイン残基を含有するタンパク質をリフォールディングする方法
DE10105912A1 (de) * 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Rekombinante Proteinase K
DE10105911A1 (de) 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Expression der rekombinanten Proteinase K aus Tritirachium album in Hefe
DE102005033250A1 (de) 2005-07-15 2007-01-18 Bioceuticals Arzneimittel Ag Verfahren zur Reinigung von G-CSF
DE202006020194U1 (de) 2006-03-01 2007-12-06 Bioceuticals Arzneimittel Ag G-CSF-Flüssigformulierung
CN101506222B (zh) 2006-07-14 2013-10-02 健泰科生物技术公司 重组蛋白的重折叠
WO2008076933A2 (en) 2006-12-14 2008-06-26 Bolder Biotechnology, Inc. Long acting proteins and peptides and methods of making and using the same
NZ601759A (en) 2010-03-17 2013-07-26 Biogenerix Gmbh Method for obtaining biologically active recombinant human g-csf
AU2011317021B2 (en) * 2010-10-20 2015-07-09 Medimmune, Llc Methods for processing inclusion bodies
HUP1200172A2 (en) 2012-03-19 2013-10-28 Richter Gedeon Nyrt Methods for refolding g-csf from inclusion bodies
HUP1200171A1 (hu) 2012-03-19 2013-09-30 Richter Gedeon Nyrt Módszerek polipeptidek elõállítására
CN103852527B (zh) * 2012-12-05 2015-05-13 中国科学院大连化学物理研究所 一种高通量蛋白质样品预处理装置
US10457716B2 (en) 2014-08-06 2019-10-29 University Of Notre Dame Du Lac Protein folding and methods of using same
CA3044371C (en) 2016-12-30 2024-01-16 Biogend Therapeutics Co., Ltd. Recombinant polypeptides and nucleic acid molecules, compositions, and methods of making and uses thereof
HRP20251064T1 (hr) 2020-12-18 2025-11-07 Richter Gedeon Nyrt. Metode za pročišćavanje ponovno savijenog fc-peptidnog fuzionog proteina

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US4468633A (en) 1982-04-28 1984-08-28 The Bendix Corporation Adjustable microwave power combiner for a plurality of coaxially mounted impatt diodes
IL68561A (en) 1982-05-05 1991-01-31 Genentech Inc Preparation of polypeptide with human tissue plasminogen activator function,processes for making it,and dna and transformed cell intermediates thereof
US4432895A (en) * 1982-11-24 1984-02-21 Hoffmann-La Roche Inc. Monomeric interferons
GR79124B (ko) * 1982-12-22 1984-10-02 Genentech Inc
JPH06102034B2 (ja) * 1983-03-25 1994-12-14 セルテク リミテツド タンパク質の生産方法
US4530787A (en) * 1984-03-28 1985-07-23 Cetus Corporation Controlled oxidation of microbially produced cysteine-containing proteins
US4748234A (en) * 1985-06-26 1988-05-31 Cetus Corporation Process for recovering refractile bodies containing heterologous proteins from microbial hosts
US4766205A (en) * 1985-11-13 1988-08-23 Beatrice Companies, Inc. Method for isolation of recombinant polypeptides in biologically active forms
FR2596360B1 (fr) * 1986-04-01 1989-02-17 Sotralentz Sa Conteneur sur palette avec dispositif de protection en treillis plie et renforce
JPH0651119A (ja) * 1992-07-28 1994-02-25 Sekisui Chem Co Ltd 位相差板の製造方法

Also Published As

Publication number Publication date
IL80325A (en) 1992-06-21
DE3650449D1 (de) 1996-01-25
EP0219874A2 (de) 1987-04-29
EP0393725B1 (de) 1995-12-13
JPS62502895A (ja) 1987-11-19
HK153496A (en) 1996-08-16
IE62634B1 (en) 1995-02-22
FI94050C (fi) 1995-07-10
JPH0824594B2 (ja) 1996-03-13
DK175109B1 (da) 2004-06-07
DK320387A (da) 1987-06-23
EP0393725A1 (de) 1990-10-24
SK278317B6 (en) 1996-10-02
HRP921075B1 (en) 1999-02-28
FI95578B (fi) 1995-11-15
FI95578C (fi) 1996-02-26
ES2061434T3 (es) 1994-12-16
CZ280727B6 (cs) 1996-04-17
SI8611796A (sl) 1996-10-31
JPH04218387A (ja) 1992-08-07
JPH0728745B2 (ja) 1995-04-05
FI872753L (fi) 1987-06-22
CA1329157C (en) 1994-05-03
GR3018410T3 (en) 1996-03-31
HUT43643A (en) 1987-11-30
EP0253823A1 (de) 1988-01-27
HU204855B (en) 1992-02-28
FI933868L (fi) 1993-09-03
KR900009139B1 (ko) 1990-12-22
FI872753A0 (fi) 1987-06-22
ATE131489T1 (de) 1995-12-15
IE862683L (en) 1987-04-23
SK752686A3 (en) 1996-10-02
WO1987002673A2 (en) 1987-05-07
EP0219874B1 (de) 1993-12-15
DE3537708A1 (de) 1987-04-23
ZA868012B (en) 1987-06-24
SU1607689A3 (ru) 1990-11-15
AU590029B2 (en) 1989-10-26
AU607083B2 (en) 1991-02-21
AU6599386A (en) 1987-05-19
HK153596A (en) 1996-08-16
DK320387D0 (da) 1987-06-23
WO1987002673A3 (en) 1987-10-22
ATE98648T1 (de) 1994-01-15
SI8611796B (sl) 1998-06-30
GR920300062T1 (en) 1992-08-31
EP0219874A3 (en) 1988-02-10
ES2020498T3 (es) 1996-04-01
IL80325A0 (en) 1987-01-30
DE3689404D1 (de) 1994-01-27
FI94050B (fi) 1995-03-31
YU179686A (en) 1988-06-30
DD260517A5 (de) 1988-09-28
ES2020498A4 (es) 1991-08-16
CZ752686A3 (en) 1996-01-17
DE3537708C2 (ko) 1993-07-08
AU4132189A (en) 1990-01-04
UA6023A1 (uk) 1994-12-29
DK200001897A (da) 2000-12-18
PT83609B (pt) 1988-10-14
FI933868A0 (fi) 1993-09-03
HRP921075A2 (en) 1995-06-30
DK175091B1 (da) 2004-05-24
PT83609A (de) 1986-11-01
YU47185B (sh) 1995-01-31

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