[go: up one dir, main page]

DE3686654D1 - Verfahren zur isolierung rekombinanter polypeptide in biologisch aktiver form. - Google Patents

Verfahren zur isolierung rekombinanter polypeptide in biologisch aktiver form.

Info

Publication number
DE3686654D1
DE3686654D1 DE8686906674T DE3686654T DE3686654D1 DE 3686654 D1 DE3686654 D1 DE 3686654D1 DE 8686906674 T DE8686906674 T DE 8686906674T DE 3686654 T DE3686654 T DE 3686654T DE 3686654 D1 DE3686654 D1 DE 3686654D1
Authority
DE
Germany
Prior art keywords
polypeptide
disulfide bonds
reducing
disulfide
biologically active
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE8686906674T
Other languages
English (en)
Other versions
DE3686654T2 (de
Inventor
Pradip Ghosh-Dastidar
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
International Genetic Engineering Inc
Original Assignee
International Genetic Engineering Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by International Genetic Engineering Inc filed Critical International Genetic Engineering Inc
Application granted granted Critical
Publication of DE3686654D1 publication Critical patent/DE3686654D1/de
Publication of DE3686654T2 publication Critical patent/DE3686654T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/43—Sweetening agents, e.g. thaumatin, monellin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE8686906674T 1985-11-13 1986-10-28 Verfahren zur isolierung rekombinanter polypeptide in biologisch aktiver form. Expired - Fee Related DE3686654T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US06/797,473 US4766205A (en) 1985-11-13 1985-11-13 Method for isolation of recombinant polypeptides in biologically active forms
PCT/US1986/002264 WO1987002985A1 (en) 1985-11-13 1986-10-28 Method for isolation of recombinant polypeptides in biologically active forms

Publications (2)

Publication Number Publication Date
DE3686654D1 true DE3686654D1 (de) 1992-10-08
DE3686654T2 DE3686654T2 (de) 1993-02-18

Family

ID=25170929

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8686906674T Expired - Fee Related DE3686654T2 (de) 1985-11-13 1986-10-28 Verfahren zur isolierung rekombinanter polypeptide in biologisch aktiver form.

Country Status (8)

Country Link
US (1) US4766205A (de)
EP (1) EP0246279B1 (de)
JP (1) JPS63501291A (de)
AT (1) ATE80166T1 (de)
AU (1) AU582927B2 (de)
CA (1) CA1265461A (de)
DE (1) DE3686654T2 (de)
WO (1) WO1987002985A1 (de)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8508340D0 (en) * 1985-03-29 1985-05-09 Creighton T E Production of protein
DE3537708A1 (de) * 1985-10-23 1987-04-23 Boehringer Mannheim Gmbh Verfahren zur aktivierung von t-pa nach expression in prokaryonten
CA1310923C (en) * 1985-11-13 1992-12-01 Joachim Ludwig Weickmann Dna encoding [asp --] and [lys -, asp --] thaumatin i
DE3618817A1 (de) * 1986-06-04 1987-12-10 Behringwerke Ag Verfahren zur gewinnung aktiver proteine aus einer biologisch inaktiven form
US4929700A (en) * 1987-04-16 1990-05-29 Cetus Corporation Production of purified, biologically active, bacterially produced recombinant human CSF-1
CA1339757C (en) * 1987-04-16 1998-03-17 Robert F. Halenbeck Production of purified biologically active, bacterially produced recombinant human csf-1
US4931543A (en) * 1987-05-11 1990-06-05 Cetus Corporation Process for recovering microbially produced interleukin-2
US5162507A (en) * 1987-05-11 1992-11-10 Cetus Corporation Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms
US4985544A (en) * 1987-08-04 1991-01-15 Kyowa Hakko Kogyo Co., Ltd. Process for renaturing fish growth hormone
JPH01132598A (ja) * 1987-10-14 1989-05-25 Pitman Moore Inc 変性剤溶液中に含まれる組換え蛋白質における分子内ジスルフィド結合の生成を促進する方法
US5079230A (en) * 1988-09-12 1992-01-07 Pitman-Moore, Inc. Stable bioactive somatotropins
DE3835350A1 (de) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
US5221624A (en) * 1988-11-08 1993-06-22 International Genetic Engineering, Inc. DNA encoding (Lys46, Asp97, Asp113) and (Lys46, Asp.sup.137) thaumatin I polypeptides
US5191063A (en) * 1989-05-02 1993-03-02 University Of Medicine And Dentistry Of New Jersey Production of biologically active polypeptides by treatment with an exogenous peptide sequence
IL96682A0 (en) * 1989-12-15 1991-09-16 Amgen Inc Production of biologically active platelet-derived growth factor from high expression host cell systems
US5986070A (en) * 1990-04-06 1999-11-16 Amgen Inc. Production of biologically active NGF proteins
US5235043A (en) * 1990-04-06 1993-08-10 Synergen, Inc. Production of biologically active, recombinant members of the ngf/bdnf family of neurotrophic proteins
AU8417091A (en) * 1990-08-20 1992-03-17 Novo Nordisk A/S Biologically active compound, a process for the preparation thereof and use of the same
AU664021B2 (en) * 1990-09-05 1995-11-02 Natinco Nv Solubilization of proteins in active forms
US5235041A (en) * 1990-12-28 1993-08-10 Protein Polymer Technologies, Inc. Purification of structurally ordered recombinant protein polymers
US5326580A (en) * 1993-02-16 1994-07-05 Wisconsin Alumni Research Foundation Brazzein sweetener
EP0628573A1 (de) * 1993-05-24 1994-12-14 Kao Corporation Verfahren zur Herstellung solubilisierten Proteins
AU2603995A (en) * 1994-05-25 1995-12-18 University Of Nebraska Board Of Regents Biologically active glycoprotein hormones produced in procaryotic cells
US5563057A (en) * 1994-10-31 1996-10-08 Wisconsin Alumni Research Foundation Method for refolding misfolded enzymes with detergent and cyclodextrin
US5789547A (en) * 1995-06-07 1998-08-04 Celtrix Pharmaceuticals, Inc. Method of producing insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-3 (IGFBP-3) with correct folding and disulfide bonding
TR199802584T2 (xx) * 1996-06-11 1999-03-22 Boehringer Mannheim Gmbh Denat�re proteinin aktive edilmesi i�in y�ntem.
ZA9711733B (en) 1996-12-31 1998-07-01 Monsanto Co Method for solubilization and naturation of somatotropins
US5993627A (en) * 1997-06-24 1999-11-30 Large Scale Biology Corporation Automated system for two-dimensional electrophoresis
US6068993A (en) * 1997-07-02 2000-05-30 Biobras Sa Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin
PL374045A1 (en) * 2001-02-23 2005-09-19 Immunex Corporation Efficient recovery of correctly refolded proteins
WO2003046195A1 (en) * 2001-11-30 2003-06-05 Novozymes A/S Method for generating a site-specific library of variants
CA2457262A1 (fr) * 2003-02-11 2004-08-11 Centre National En Electrochimie Et En Technologies Environnementales In C. Procede et dispositif pour la production de biomolecules en continu
SG156672A1 (en) * 2004-10-22 2009-11-26 Amgen Inc Methods for refolding of recombinant antibodies
EP4512422A3 (de) * 2007-09-14 2025-05-21 Amgen Inc. Homogene antikörperpopulationen
CN107074968B (zh) * 2014-09-01 2021-03-12 国际遗传工程和生物技术中心 疫苗
AR103172A1 (es) 2014-12-22 2017-04-19 Novartis Ag Reducción selectiva de residuos de cisteina en anticuerpos il-17
CN119775339B (zh) * 2025-03-13 2025-09-12 吉尔生化（上海）有限公司 一种提高固相合成的含多个半胱氨酸多肽稳定性的方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE55163B1 (en) * 1982-06-07 1990-06-20 Celltech Ltd A process for the preparation of chymosin
AU538170B2 (en) * 1982-09-15 1984-08-02 Owens-Illinois Glass Container Inc. Modified protein to mould an enzyme type molecule
US4511502A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4518526A (en) * 1982-12-22 1985-05-21 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4512922A (en) * 1982-12-22 1985-04-23 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4599197A (en) * 1982-12-22 1986-07-08 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
GR79124B (de) * 1982-12-22 1984-10-02 Genentech Inc
US4511503A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
WO1984003711A1 (en) * 1983-03-25 1984-09-27 Celltech Ltd A process for the production of a protein
JPS6079000A (ja) * 1983-10-04 1985-05-04 Takeda Chem Ind Ltd ヒトγ型インタ−フエロン単量体の製造法
EP0155832A3 (de) * 1984-03-16 1987-08-19 Genentech, Inc. Stabile Proteine, Verfahren zu deren Herstellung, dieselben kodierende Plasmide und andere DNS, diese Plasmide enthaltende Zellkulturen
US4569790A (en) * 1984-03-28 1986-02-11 Cetus Corporation Process for recovering microbially produced interleukin-2 and purified recombinant interleukin-2 compositions
US4530787A (en) * 1984-03-28 1985-07-23 Cetus Corporation Controlled oxidation of microbially produced cysteine-containing proteins
DE3537708A1 (de) * 1985-10-23 1987-04-23 Boehringer Mannheim Gmbh Verfahren zur aktivierung von t-pa nach expression in prokaryonten

Also Published As

Publication number Publication date
DE3686654T2 (de) 1993-02-18
AU6591686A (en) 1987-06-02
CA1265461A (en) 1990-02-06
EP0246279A1 (de) 1987-11-25
EP0246279A4 (de) 1989-05-11
ATE80166T1 (de) 1992-09-15
WO1987002985A1 (en) 1987-05-21
JPS63501291A (ja) 1988-05-19
US4766205A (en) 1988-08-23
EP0246279B1 (de) 1992-09-02
AU582927B2 (en) 1989-04-13

Similar Documents

Publication Publication Date Title
DE3686654D1 (de) Verfahren zur isolierung rekombinanter polypeptide in biologisch aktiver form.
DE3650751D1 (de) Antikörper gegen Saugetier-Interleukin-4 und Peptide verwendbar als Antigenen für deren Herstellung
ES2020498T3 (es) Procedimiento para la activacion de proteinas eucarioticas heterologas, que tienen puentes de disulfuro, preparadas por tecnologia genetica, despues de su expresion en el seno de procariotas.
DK0458959T3 (da) Hidtil ukendte blodpladeafledte vækstfaktor B-kædeanaloger og fremgangsmåde til homogen fremstilling deraf
DK0643767T3 (da) Osteogene peptider
AU5385486A (en) Biologically active protein compositions and a method of use
CA1267497C (en) PROTEIN RECOVERY
ES548617A0 (es) Procedimiento para disociar peptidos y proteinas en el enla-ce metionilo
ES2065907T3 (es) Procedimiento para la produccion de un peptido fisiologicamente activo que contiene un resto cisteina.
PT93178A (pt) Agente proteico anticanceroso
MX9302509A (es) Composiciones de polipeptidos estables en almacenamiento y metodo para su preparacion.
DK0496973T3 (da) Porcine somatotropiner med ændringer i alfa-helix 1 regionen og kombinationer med andre mutationer.
DE3873273D1 (de) Verfahren zur selektiven spaltung von fusionsproteinen.
DE69121489D1 (de) In vitro-Behandlung von Fusionsproteinen
DK34590D0 (da) Biologisk aktive molekyler
DE59503776D1 (de) Cyclohexapeptide und deren Mischungen, Verfahren zur Herstellung sowie deren Verwendung
SE9101586L (sv) Modifierat tioredoxin och dess anvaendning

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee