RU2667781C1 - Biologically active cedar shell additive and method for its preparation - Google Patents

Abstract

FIELD: biotechnology.SUBSTANCE: invention relates to creation of effective preparations from plant raw materials and can be used in the food, fodder perfume and cosmetic industry. Biologically active additive (BAA) for food, fodder and perfumery-cosmetic purposes includes a cedar nut shell, subjected to mechanochemical treatment. In this case, the shell of pine nuts is subjected to mechanochemical treatment in a vibratory mill to an average particle size of 2.0–9.0 mcm at a temperature not exceeding 50 °C. BAA is characterized by a certain content of moisture, protein, fat, nitrogen-free extractives, ash, calcium, phosphorus and fiber.EFFECT: invention allows to obtain a finished product with enhanced biological efficiency by increasing the amount of bioavailable useful components.11 cl, 3 tbl

Description

The invention relates to the creation of effective preparations from plant materials - cedar shells as a means for the prevention of certain human diseases, in addition to the food industry, in particular to the production of preventive products, to the production of feed additives for animals, to the perfume and cosmetics industry.

The urgent problem of preventive medicine is the search for new effective means and methods of rehabilitation and recovery of the body.

The healing properties of Siberian cedar have been known for a very long time. Almost all parts of the plant are used as medicinal raw materials - needles, young shoots, bark, gum. Of great interest is the shell of pine nuts - as a source of biologically active substances with medicinal properties. The bulk of pine nutshells are carbohydrates (in the form of cellulose, hemicellulose, pentosans) and lignin. Cellulose, hemicellulose, lignin have a mild sorption effect.

Known means for the prevention and treatment of musculoskeletal system (patent RU 2458697, IPC A61K 36/532; A61K 36/15; A61P 19/02, publ. 08.20.2012) containing the active substance in the form of crushed raw pine nuts (kernel, film, shell), leafy shoots of hyssop officinalis and isopropyl 40-50% alcohol and, according to the second option, crushed raw pine nuts (kernel, film, shell, shavings, sawdust, wood chips), leafy shoots of hyssop medicinal and ethyl 40-50% alcohol .

The disadvantage of the tool is the complex composition, which increases the cost and at the same time the degree of grinding of pine nuts (kernel, film, shell) is not controlled, which reduces the effectiveness of the tool when used.

Known feed additive with immunostimulating activity (patent RU 2305953, IPC A23K 1/00; A23K 1/16, publ. 09/20/2007), which contains pine nut shell powder, succinic, citric and ascorbic acid in the following ratio of ingredients, weight. %: pine nut shell powder - 94.0-95.0; succinic acid 2.5-3.0; ascorbic acid - 0.8-1.0; citric acid 1.7-2.0. The feed additive has good bioavailability for the body of farm birds and stimulates immunity factors and non-specific resistance.

The disadvantage of this feed additive is the need to add various acids, which increases the cost of the product.

Known feed additive for poultry (patent RU 2228643, IPC A23K 1/00; A23K 1/16, publ. 05/20/2004), consisting of pine nuts shell (SKO) in the form of fine powder. DIS is a complex natural organic-mineral complex, including several tens of various chemical compounds.

The disadvantage of the feed additive is that the particle size is not controlled when grinding the shell, which reduces the bioavailability of the additive components and its effect on the bird and reduces the effectiveness of the feed additive.

Known cosmetic cleaning mask for facial skin care (patent RU 2120273, IPC A61K 7/00; A61K 7/48, publ. 10/16/1996), including a base, finely divided mineral and / or vegetable sorbents and biologically active substances of plant origin, according to According to the invention, the egg protein, the balanced salt composition of Hanks solution, the amino acid vitamin complex (AVK) of the nutrient medium for animal cell cultures, for example, Needle, a preservative are added to the mask, and a gelling agent is used as the base .

As a finely divided plant sorbent, ground peach bones and / or crushed pine nuts shell with an average particle size of not more than 20 microns are used. Cytotoxicity and antiviral activity were not determined.

The disadvantage of this mask is that the temperature during grinding of the shell of pine nuts (RMS) is not regulated, which reduces the biological activity of RMS.

The closest analogue to the claimed solution is a biologically active additive (patent RU 2236154, IPC A23L 1/30; A23L 1/29; A61K 7/00, publ. 09/20/2004) containing cedar nut shell powder (SKO) obtained in a certain way . The powder has a particle size of 10-1200 μm, where the lipid content is up to 9.7 wt. %, flavonoids up to 1.4 wt. %, tannins up to 9.7 wt. %, proteins up to 6.9 wt. %, polysaccharides 57.0-58.0%, potassium approximately 3300 mg / kg, phosphorus approximately 680 mg / kg, magnesium approximately 650 mg / kg, iron approximately 140 mg / kg, calcium approximately 80 mg / kg, zinc - approximately 35 mg / kg, copper - approximately 2.6 mg / kg. The powder is obtained by grinding the shells of pine nuts. The grinding is carried out by forming two counter flows of dried raw materials with a given speed of movement of the raw materials in the stream from 100 to 450 m / s with the possibility of collision of particles of raw materials in a limited area at a temperature not lower than the pasteurization temperature. EFFECT: invention makes it possible to introduce pine nutshell powder into these products obtained by the developed method, aimed at forming the optimal composition of pine nut shell powder by controlling the intensity of the reaction of the components between each other.

The disadvantage of this additive is that it is obtained at a temperature not lower than the pasteurization temperature, which leads to the loss of useful properties of some components, due to the temperature increase above 90 ° C, for example, the activity of vitamin P is lost. At high flow rates, SKO particles can ignite and completely lose all the useful components.

The objective of the invention is the creation of a biologically active additive based on a pine nut shell (SKO) for prophylactic, food, feed purposes with higher biological efficiency due to mechanochemical processing and the method for its preparation.

The problem is solved with the help of biologically active additives for food and feed purposes, for preventive and perfumery and cosmetic purposes, including cedar nutshell, subjected to mechanochemical processing. The pine nut shell is subjected to mechanochemical treatment in a vibratory mill to an average particle size of 2.0–9.0 μm at a temperature not exceeding 50 ° C and the additive has the following composition, wt. %:

Moisture 8.61-9.00 Protein 5.00-5.10 Fat 6.00-9.50 Bev 24.00-27.00 Ash 1.00-1.30 Calcium 0.15-0.30 Phosphorus 0.08-1.10 Cellulose 50.00-52.00

Preferably, the additive contains the following trace elements, mg / kg:

Potassium 3600,00 Sodium 70.00 Magnesium 740.00 Iron 192.00 Manganese 60.00 Copper 4,50 Zinc 30.00

Preferably, the additive contains the following amino acids,%:

Asparagine 0.36 Threonine 0.14 Serine 0.14 Glutamine 0.44 Glycine 0.16 Alanine 0.16 Valine 0.15 Methionine 0.15 Isoleucine 0.12 Leucine 0.23 Tyrosine 0.08 Phenylalanine 0.13 Histidine 0.06 Lysine  0.16 Arginine 0.16

Preferably, the additive is suitable for the preparation of baths at the rate of 25 g of the additive per 200 l of water.

Preferably, the additive is suitable for use as an application by mixing in an alcohol solution or hot water until the consistency of thick sour cream.

Preferably, the additive is suitable for use as a dietary supplement and for therapeutic purposes intended for human consumption by diluting 1 teaspoon in 0.5 cups of water.

Preferably, the additive is suitable for use as a feed additive intended for animal consumption by adding to the feed mixture in an amount of 1% of the diet, calculated on the dry matter.

Preferably, the additive has antiviral activity, a high level of cytotoxicity and retains its properties for a long time.

The problem is solved in the same way using a method of obtaining a biologically active additive for food and feed purposes, for preventive and perfumery-cosmetic purposes, including mechanochemical processing of pine nuts shells. The mechanochemical treatment is carried out in a vibratory mill at a temperature not exceeding 50 ° C to an average particle size of 2.0–9.0 μm.

Preferably, external cooling of the vibratory mill with cold water is used.

Preferably, the mechanochemical treatment is carried out for a time which ensures grinding to a predetermined particle size.

The most effective was the processing of RMS in high-energy-intensive grinding mills-activators, preferably in vibratory mills, in which the amount of bioavailable useful components, vitamins, enzymes increases, their losses during processing in vibratory mills with additional cooling of the grinding chamber are reduced (there is no uncontrolled rise in temperature).

Pine nut shell (RMS) is subjected to mechanochemical treatment to a predetermined particle size, and preferably to an average particle size of 2.0-10.0 microns, in apparatuses (activator mills), which can be used as vibration mills.

The mechanochemical treatment in vibratory mills does not lead to the loss of biologically active components, since the temperature does not rise above 50 ° C. The composition of the obtained powder SKO moisture content reaches 9 wt. %, which is one of the confirmation that the temperature in the chamber does not rise above 50 ° C.

During mechanochemical treatment in a vibratory mill, partial surface amorphization of the crushed shell surface occurs, many defects appear, and the surface is thermodynamically activated, but not electrified, which simplifies the further use of the additive.

The vibrating mill consists in the fact that the vibration mill produces a great many shock effects, while a single impact has a small force and the temperature does not rise above 50 ° C during grinding of the standard deviation.

To maintain the required temperature in the activator mill, any devices and methods used for these purposes and known from the prior art can be used.

It is possible to install sensors to control the temperature in the mill, using external cooling of the activator, for example, cold water.

When processing SKO according to the prototype, SKO powder does not contain moisture, which confirms the temperature rise above 100 ° C.

It was not obvious from the prior art that at low temperatures of mechanochemical processing not higher than 50 ° C, the resulting RMS powder will have a high level of cytotoxicity and antiviral activity.

In the proposed solution, the vibratory mill may have additional cooling with water, which prevents the loss of useful components.

Thus, in order to guarantee the preservation of all the useful properties of DIS, the conditions for the proposed mechanochemical treatment are not obvious and were not selected by a simple enumeration of the conditions for its implementation.

All chemical reactions taking place during mechanochemical processing of the shell were taken into account at various temperatures, which ultimately influenced the composition of the powder from the cedar shell after processing.

During mechanochemical treatment, the destruction of macrostructural elements, in particular cell walls consisting of cellulose, and cell membranes is achieved, which ensures the bioavailability of useful water-soluble components of the standard dispersive powder.

During mechanochemical treatment in a vibratory mill at a temperature not exceeding 50 ° C, a large number of amino acids and vitamins are formed (table 1). The resulting composition of the RMS powder includes water-soluble and fat-soluble vitamins, flavonoids and other components that are contained in a bioavailable form and are extracted with water, an alcohol solution or physiological media, such as gastric juice.

The resulting SKO powder contains fat-soluble vitamins (vitamins E), water-soluble vitamins (group B) and trace elements (copper, iron, manganese, phosphorus, etc.), which makes it suitable as a feed additive, food supplement, and other purposes. Thanks to the obtained composition of the powder, the SKO eliminates the need to introduce large quantities of expensive vitamins into animal feed.

The obtained SKO powder contains in large quantities the most essential amino acids (lysine, arginine, methionine) in the diet of animals.

Fine powder SKO has a light brown to chocolate color with a specific bitter smell and a taste of pine nuts, with a mass fraction of extractives extracted with water - up to 20% or more. Extractive substances include tannins, gums, resins, essential oils, gum.

SKO powder, a biologically active food supplement, can be successfully used for direct consumption as an independent prophylactic or against the background of general diet therapy, as well as for preparing various functional foods. Preferably, the use of powders with an average particle size of 2.0-10.0 μm leads to an increase in the digestibility of the components, an increase in biological activity due to an increase in the surface area of the particles and the intensification of the extraction process.

The resulting powder of pine nutshells is a complex natural organic-mineral complex, including several tens of various chemical compounds in a bioavailable form. The effect is made by carbon, represented by poly - (fiber, pectin substances) and monosaccharides, and trace elements.

Fiber is the basis of the structure of the cell wall of the cedar shell and is represented by hemicelluloses, cellulose and associated lignin. Nitrogen-free extractive substances (BEV) - provide a diverse mixture of hydrocarbons (except fiber) and other substances that do not contain nitrogen and the content of BEV reaches up to 27.0 mass. %

The composition of BEVs includes sugar, starch, hemicelluloses, pectin substances, pigments, resins, tannins, organic acids.

The therapeutic, stimulating and preventive effect of the proposed biologically active additives on the human body, animal is associated with the presence of biologically active substances of various composition in it:

- tannins or tannides, have astringent and anti-inflammatory effects and help with poisoning by heavy metals and alkaloids;

- flavonoids, have antioxidant activity, the ability to strengthen the walls of blood capillaries;

- amino acids, have a positive physiological effect on the human body, animal, on the digestive system;

- glycosides, nitrogen-free extractive substances (BEV), consisting of sugar (glycogen) and non-sugar (aglycon) parts and support the work of the heart muscle, positively affect the work of the gastrointestinal tract.

The combined effect of the above active substances contained in the proposed biologically active additive activates all the important processes in the human body, animal.

The mechanochemical treatment of RMS at a temperature of no higher than 50 ° C ensures the effective extraction of all therapeutic components contained in it, including micro and macro elements, has antiviral activity and a high level of cytotoxicity, and has a more effective effect on the human body and animal.

Information about the effectiveness of the obtained additives, as well as specific examples of its preparation are given below.

In the following examples, a vibratory mill for fine grinding was used with the following characteristics:

- productivity, l / hour, adjustable 2-200 - the number of grinding chambers, pcs. 2 - capacity of the grinding chamber, l, not less thirty - type of grinding dry - grinding bodies rods and balls - type of vibration exciter unbalanced - waveform circular - cooling agent water - temperature of the cooling agent, ° C no more than 15.

Due to the possible aggregation of RMS powders, they cannot be properly dispersed in the liquid, which leads to a decrease in their effectiveness or may require additional time or effort to achieve sufficient dispersion of the powder in the liquid.

The positive result is that the use of a vibratory mill for mechanochemical processing prevents aggregation of the RMS powder, and the powder retains its properties for a long time.

Example 1

The air-dry shell of Siberian cedar was used as a pine nutshell. A shell of pine nuts was fed into a mechanochemical reactor. A vibratory mill was used as a mechanochemical reactor. In a vibration mill, mechanochemical treatment was carried out for 40 minutes to an average particle size of 2.45 μm. The temperature was maintained no higher than 45 ° C. The resulting powder was used as a nutritional supplement and therapeutic purposes, its composition and indicators are shown in table 1.

Example 2

Similar to example 1.

Mechanochemical treatment was carried out in a vibratory mill for 30 minutes to an average particle size of 5.0 μm. The resulting powder was used as a feed additive.

Example 3

Similar to example 1.

Raw materials were processed in a vibratory mill for 20 min to an average particle size of 9.0 μm. The resulting powder was used as a food additive and perfumery and cosmetic purposes.

Example 4. (prototype).

The particle size is 100 μm, the processing temperature is 100 ° C.

Moisture, B vitamins were not found in the composition of the additive, the SKO powder clumped in air.

The results of determining the parameters of the extraction of powder according to example 1 (yield of extractive substances and the content of tannins) are shown in table 2.

Three concentrations of ethyl alcohol of 40%, 50% and 70% were tested. The same weight of the RMS powder according to Example 1 was poured with the same volume of alcohol of the corresponding concentration and insisted for 1-8 hours. The completeness of the extraction was determined by the yield of extractive and tannins in% (table 2).

* - time - 1 hour; ** - time - 6 hours.

From table 2 it follows that the alcohol with a concentration of 50% is optimal for extraction - in terms of extractive substances and quite satisfactory - in terms of the yield of tannins, 6 hours is the optimal time for insisting of SKO powder. A slight increase in the content of extractives after 8 hours is not significant.

Thus, the optimal conditions for the extraction of substances from the powder of the shell of pine nuts is the infusion for 6 hours with the use of 50% ethyl alcohol. The use of 70% alcohol does not give advantages.

In 1 ml of the solution in example 1 contains more substances than in example 4, which confirms the effectiveness of the proposed additives.

Tests for cytotoxicity and antiviral activity are given in the results of the test of the powder of the RMS for perfume and cosmetic purposes.

Below are the test results of the proposed additives.

1. Therapeutic and prophylactic use of pine nut shell powder for the preparation of bathtubs and applications.

Methodology of the procedures.

Bathtubs - the water temperature should be 37-38 ° C, a single dose of SKO powder according to the example 1-25 g (2 tablespoons) per 200 liter bath is brewed in a small amount of hot water until a pasty consistency, stand for 5-7 minutes, then stir in the bath. The duration of the procedure is 15 minutes.

Applications were carried out in a sauna. First, the area of the lymphatic region of the affected joint is prepared for therapeutic effect: the patient is warmed up, then lymph-stimulating massage is performed - rubbing of the affected skin areas with special salts for saunas and baths; another massage option is possible - the use of birch or oak brooms. Next, the actual application effect of the finely dispersed fraction of cedar shell powder is carried out.

The required amount of powder is brewed in a small amount of hot water until the consistency of thick sour cream, after 5-7 minutes an application is carried out. The mixture is applied to the lymphatic region of the affected joint and simultaneously to the vertebral region for 20-30 minutes, then the powder is washed off with warm water.

The course of treatment is 3 weeks.

The results of the study showed that according to subjective data, there was an improvement in both the general condition and the condition of the musculoskeletal system. Moreover, the positive effect persisted in the long term - 3-4 weeks after discharge from the hospital. According to bioelectrography, improvement occurred in 76.5% of patients, respectively. The positive therapeutic effect of bathtubs and applications of finely divided fractions of cedar shells can be due to a number of factors that act when using mineral baths and mud applications - thermal, mechanical, hydrostatic, chemical. Minerals, vitamins, amino acids, fat-soluble compounds penetrate the skin into the internal environment of the body and contribute to the improvement of trophism of periarticular tissues, resorption of seals, increase the elasticity of the joint capsule and ligaments of the affected joints. Present in the SKO fraction, fiber, lignin act on the skin as sorbents, absorbing bacteria, inorganic and organic compounds, so that the skin is cleaned, its absorption capacity increases. Moreover, these same factors will affect the region of the lymphatic region of the affected joint, contributing to its more complete rehabilitation.

Biologically active substances dissolved in water are a kind of irritant that acts on the receptors, blood and lymph vessels of the skin. Bathtubs change the functional state of various organs and systems of the body and have a stimulating or normalizing effect on adaptation systems - neuroendocrine, immune, lymphatic, circulatory.

Then, the methodology for the use of the studied agent was changed. Preferably, the applications were alternated every other day with baths. The application mixture was prepared on water, which was a prerequisite for a milder effect. In addition, to prevent the progression of endotoxemia, a set of methods was used that constitute the essence of endoecological rehabilitation and aimed at enhancing the drainage and detoxification function of the lymphatic system and other excretory organs.

Conclusion: baths with a finely divided fraction of cedar shells are, on the one hand, a treatment factor, in relation to the underlying disease, and on the other hand, an important tool for endoecological rehabilitation, since they stimulate the removal of toxic products from the internal environment of the body through the skin, thereby relieving the lymphatic system, liver and the kidneys. The appointment of the above procedures allowed to avoid the appointment of painkillers and achieve a good therapeutic effect in the long term - 3-4 weeks after discharge from the hospital.

The use of finely divided fractions of cedar shells in the form of baths and applications is an effective tool in the treatment and rehabilitation of patients with degenerative joint diseases.

The duration of treatment should be at least three weeks.

The effect of treatment in some patients can be delayed and is maximally manifested 3-4 weeks after discharge from the hospital.

Degenerative diseases of the musculoskeletal system - osteoarthritis of any localization, spinal osteochondrosis are some of the indications for external use of the specified means in the form of baths and applications.

2. Testing the powder of the shell of pine nuts as a feed additive for farm animals.

The studies were conducted under the conditions of modern industrial technology.

Given the presence in the powder of pine nutshell biologically active components such as lipids, flavonoids, tannins, protein, vitamins, micro and macro elements and especially poly and monosaccharides, which contain up to 60%, tests have been carried out.

In the experimental group of piglets from 1 to 28 days of life, they received a decoction of the powder of DIS with the help of Example 2, and in the control group, drinking water.

It was established that the piglets of the experimental groups readily drank (even licked the rest of the powder) a decoction of the pine nut shell, from the first days of life and to weaning they were better preserved. So, when weaning piglets at 28 days in the experimental group, the safety was higher by 2.8% compared with the control, where the piglets received only drinking water.

In addition, when transferring to rearing in the experimental group, there were 8.1% less lagging behind in the development of piglets than in the control.

It should also be noted that increased taste interest in additives from pine nutshell shells makes it possible to carry out an effective way of introducing vitamin and drugs into the body of piglets. RMS has 0.35 feed units, the exchange energy is 4.09 MJ

Thus, the use of cedar shell powder, even when fully fed under modern technology, is of practical interest and it is recommended to add about 1% of the additive in terms of dry matter to the feed mixture. The amount of additive in the feed mixture may vary depending on the season, animal welfare conditions, etc.

3. Testing the powder of the standard disperse for perfumery and cosmetic purposes.

3.1. Determination of cytotoxicity, antiviral activity and the range of concentrations of the extract in the final composition of the cosmetic product.

The method of preparation of the extract.

90 g of fine powder SKO according to example 3 is loaded into a high-speed mixer (blender), 129.4 g of deionized water and 301.7 g of ethanol (95%) are added. Intensive homogenization with simultaneous extraction is carried out for 10 minutes with a stepwise increase in speed from 1 to 5 (two minutes each). The resulting suspension is filtered through a paper filter. The obtained brightly colored filtrate - 249 g (47.8%) was passed for testing (sample code RE1-57) aimed at determining cytopathic and antiviral activity.

Assessment of cytotoxicity is carried out in two versions:

Option 1. To cells of the lungs of the human embryo (LEC), which formed a dense monolayer, add growth medium with the addition of 5% of the sample RE1-57 (experimental medium). The resulting system is kept in a thermostat at 37 ° C for 3 hours, the experimental medium is drained, the cells are washed with normal growth medium and again incubated for 24 hours in a thermostat. As a result of the experiment (counting the number of cells) in triplicate, results are obtained indicating a high level of cytotoxicity of the obtained extract.

Option 2. To a dense monolayer of green monkey kidney cells (Vero) sequentially add the preparation RE1-57, gradually increase its concentration in the nutrient medium. Cells are incubated at 37 ° C for 96 hours. Comparison of the number of cells in the experimental samples with the control samples confirms a high level of cytotoxicity. The average value of the maximum permissible dose for two parallel experiments was 0.63%.

In parallel experiments with the addition of a viral suspension (poxvirus), the presence of antiviral activity at a concentration of 0.33% was established, which indicates the blocking of the effect of viral particles on the cellular system at concentrations above 0.3%. This fact is extremely important, since currently there is no information on the antiviral activity of the vast majority of cosmetic ingredients.

Based on the studies, it is possible to recommend the range of extract concentrations in the final composition of the cosmetic product from 0.3 to 0.5% as the optimal one.

3.2. Identification of optimal concentrations of RMS powder in dry masks with determination of cytotoxicity and antiviral activity.

The method of preparation of extracts.

Initially, the basis of a dry cosmetic mask is prepared, including finely divided silica gel, starch, a set of inorganic salts in a physiological ratio and glycerin. Mixing is carried out with SKO powder with simultaneous grinding of the components in a porcelain ball mill for two hours. Then, a sample of 2.38 g was taken from the resulting mixture. In order to simulate a real method of applying a dry mask, 5.4 g of water was added to the sample and the resulting suspension was shaken with exposure at room temperature for 30 minutes. Then, 13.35 g of ethyl alcohol was added to the suspension, the resulting mixture was shaken for 5 minutes and settled. A water-alcohol clear solution (control sample TE1-28 / 1 cn) was passed to evaluate cytotoxicity.

Similarly, after a two-hour grinding of the mixture in the presence of different amounts of SKO powder, experimental samples TE1-28 / 2 sp (12% SKO) and TE1-28 / 3 sp (24% SKO) are obtained.

Assessment of cytotoxicity is carried out according to the following option.

Experimental samples are successively added to the dense monolayer of green monkey kidney cells (Vero), gradually increasing their concentration in the nutrient medium. Cells are incubated at 37 ° C for 96 hours. Comparison of the number of cells in the experimental samples with control samples indicates the absence of cytotoxicity.

In parallel experiments with the addition of a viral suspension (poxvirus), the absence of antiviral activity of the extracts was established at concentrations from 0.01 to 5.0%.

Based on the studies, it is possible to recommend the range of concentrations of the standard dispersive powder in the final composition of dry cosmetic masks from 15 to 25%.

3.3. Method of using SKO powder for the preparation of youth cleansing masks.

For oily skin (3-4 times a week) - one teaspoon of dry SKO powder (with a slide) according to example 3 was mixed with one teaspoon of boiled water or a decoction of green tea and the resulting mass was applied to the face without affecting the eyelids. The mask was kept on the face until completely dry for 30 minutes and washed off with cool water.

For skin with obvious signs of youth acne (daily for a month) - prepared a thicker mixture of two teaspoons of dry weight. Similarly, the entire surface of the face is treated. A month later, the acne disappeared.

Using the proposed SKO powder, it is possible to prepare masks with various components that have shown a good effect.

Youth cleansing mask with a whitening effect contains: starch and spherical particles of silica gel, precipitated from the solution (surface cleaning), crushed cedar shells, Chinese green tea leaves, specially treated to increase the availability of plant cell components, as well as glycerin, citric acid, urea and a set of inorganic salts in optimal proportions (deep skin cleansing). The mask showed a positive result with deep skin cleansing.

The mask is recommended for use up to 20-25 years.

The main goal of cosmetic effects at the age of 20-25 is to prevent and eliminate abnormal skin conditions that occur during puberty - youth acne, and protection from external adverse effects.

4. The use of tincture of pine nutshell:

- the tests were conducted on a group of patients suffering from periodic pains of a gastritic nature. At the beginning of the year, a preventive and treatment course was carried out, including the intake of alcohol tincture of pine nutshell powder inside, 1-2 teaspoons 3 times a day for 15 minutes before meals for 2 weeks. By the end of treatment, the patient stopped complaining of pain in the stomach. The observation of patients of this group over the next 6 months showed a complete absence in patients with gastritis pain.

- tests were carried out on a group of patients suffering from periodic pain of a joint-rheumatic nature. After ingestion 3 times a day for 15 minutes before eating for 2 weeks, 0.5 cup of water into which 1 teaspoon of SKO powder is added at 40 ° C, the pain completely disappeared. Observation of patients within 2 months after the end of treatment showed a complete absence of painful sensations in patients. In addition, none of the group of patients during the year fell ill with viral and colds;

- the tests were conducted on a group of patients suffering from periodic acute attacks of pain associated with chronic radiculitis. After rubbing with tincture before bedtime in the painful vertebral region for 1 week, there was a complete disappearance of pain. Observation of patients within 2 months after the course of treatment showed the disappearance of previously repeated pain.

Based on the tests of the proposed SKO powder, we can recommend:

- as a nutritional supplement, as a general strengthening agent, to relieve fatigue, to normalize the psychoemotional state both during over-stimulation and overwork, to restore strength after physical exertion (in the form of baths);

- as a means for skin care, prevention of premature aging, elimination of inflammatory reactions, acne, to accelerate the healing of minor injuries, bruises (in the form of baths and / or applications);

- as an anti-inflammatory and analgesic for diseases of the joints (in the form of baths and applications);

- as a venotonic agent for external and internal use (varicose veins of the lower extremities, hemorrhoids);

- with ulcerative and inflammatory lesions of the gastrointestinal tract as a means of enhancing the regeneration of the mucous membrane;

- as a feed additive.

The technical result of the proposed additives is that:

1. The biological activity of the additive from the cedar shell increases when it is used by humans.

2. Water-soluble and fat-soluble vitamins and flavonoids are contained in a bioavailable form, that is, they can be directly extracted with water or physiological media, such as gastric juice.

3. Supplement when used as a feed additive for farm animals increases the efficiency of feed use and increases productivity. The additive increases the safety and live weight of farm animals.

4. The developed additive has preventive and therapeutic properties for animals and humans, strengthens the immune system.

5. Using additives, environmentally friendly products are obtained from cheap plant materials for perfumery and cosmetic purposes with antiviral activity and a high level of cytotoxicity.

Claims (14)

1. Dietary supplement for food and feed purposes, for prophylactic and perfumery-cosmetic purposes, including a pine nut shell subjected to mechanochemical processing, characterized in that the pine nut shell is subjected to mechanochemical processing in a vibratory mill to an average particle size of 2.0-9, 0 μm at a temperature not exceeding 50 ° C and the additive has the following composition, wt. %: Moisture 8.61-9.00 Protein 5.00-5.10 Fat 6.00-9.50 Bev 24.00-27.00 Ash 1.00-1.30 Calcium 0.15-0.30 Phosphorus 0.08-1.10 Cellulose 50.00-52.00 2. The additive according to p. 1, characterized in that it contains the following micro-macrocells, mg / kg: Potassium 3600,00 Sodium 70.00 Magnesium 740.00 Iron 192.00 Manganese 60.00 Copper 4,50 Zinc 30.00 3. The additive according to claim 1, characterized in that it contains the following amino acids,%: Asparagine 0.36 Threonine 0.14 Serine 0.14 Glutamine 0.44 Glycine 0.16 Alanine 0.16 Valine 0.15 Methionine 0.15 Isoleucine 0.12 Leucine 0.23 Tyrosine 0.08 Phenylalanine 0.13 Histidine 0.06 Lysine 0.16 Arginine 0.16 4. The additive according to any one of paragraphs. 1-3, characterized in that it is suitable for the preparation of baths at the rate of 25 g of the additive per 200 l of water. 5. The additive according to any one of paragraphs. 1-3, characterized in that it is suitable for use as an application by mixing in an alcohol solution or hot water until the consistency of thick sour cream. 6. Additive according to any one of paragraphs. 1-3, characterized in that it is suitable for use as a dietary supplement and prophylactic purposes intended for human consumption by diluting 1 teaspoon in 0.5 cups of water. 7. The additive according to any one of paragraphs. 1-3, characterized in that it is suitable for use as a feed additive intended for consumption by animals by adding to the feed mixture in an amount of 1% of the diet in terms of dry matter. 8. The additive according to any one of paragraphs. 1-7, characterized in that it has antiviral activity, a high level of cytotoxicity and retains its properties for a long time. 9. The method of obtaining biologically active additives according to any one of paragraphs. 1-3 for food and feed purposes, for prophylactic and perfumery and cosmetic purposes, including mechanochemical processing of pine nutshells, characterized in that the mechanochemical processing is carried out in a vibrating mill at a temperature not exceeding 50 ° C to an average particle size of 2.0-9, 0 microns. 10. The method according to p. 9, characterized in that they use external cooling of the vibratory mill with cold water. 11. The method according to any one of paragraphs. 9, 10, characterized in that the mechanochemical treatment is carried out for a time providing grinding to a predetermined particle size.