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WO2018034595A1 - Méthode pour traiter les cicatrices atrophiques de la peau - Google Patents

Méthode pour traiter les cicatrices atrophiques de la peau Download PDF

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Publication number
WO2018034595A1
WO2018034595A1 PCT/RU2017/000595 RU2017000595W WO2018034595A1 WO 2018034595 A1 WO2018034595 A1 WO 2018034595A1 RU 2017000595 W RU2017000595 W RU 2017000595W WO 2018034595 A1 WO2018034595 A1 WO 2018034595A1
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WO
WIPO (PCT)
Prior art keywords
blood
treatment method
platelet
rich plasma
plasma
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Ceased
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PCT/RU2017/000595
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English (en)
Russian (ru)
Inventor
Сергей Борисович ТАРТАКОВСКИЙ
Елена Валериевна ЛИПОВА
Сергей Иванович СУРКИЧИН
Наталья Владимировна ГРЯЗЕВА
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14—Blood; Artificial blood
    • A61K35/16—Blood plasma; Blood serum
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B18/00—Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body
    • A61B18/04—Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body by heating
    • A61B18/12—Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body by heating by passing a current through the tissue to be heated, e.g. high-frequency current
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like

Definitions

  • the invention relates to medicine, namely to dermatology and cosmetology, and can be used to treat atrophic skin scars that have arisen due to various reasons.
  • a known method of eliminating scars (RU 2030923 C1, 03/20/1995), which consists in exposing the scar tissue to laser radiation.
  • Laser exposure is carried out by a focused beam of a holmium laser with a wavelength of 1.96 - 2.15 ⁇ m in a pulse-periodic mode with a pulse duration of 200 - 500 ⁇ s, an energy of 0.1 - 2.0 J.
  • Its disadvantage is that it is very difficult to dose the penetration depth laser beam, subsequently, a demarcation line remains at the border of the treated and untreated sections, in addition, the implementation of this method requires the use of expensive equipment.
  • a known method of treating hypotrophic skin scars which also includes the use of laser radiation (RU 2323021 C1, 04/27/2008). Scar tissue is subjected to a single exposure to laser radiation with a wavelength of 540 nm, a pulse energy of 50-150 mJ, a pulse repetition rate of 1-4 Hz, a pulse duration of 1-2 ns. This method also requires the use of expensive equipment.
  • a known method of treating normo-atrophic scars with mechanical dermabrasion using a combined collagen coating with an antimicrobial agent (RU 2230554 C2, 06/20/2004).
  • a complex of preoperative measures and dermabrasion is carried out, while the area is exposed on an outpatient basis in sterile conditions of a small operating room operations, the skin surface is treated twice with 10 ml butol using a gauze cloth, capturing more than 2-3 cm of the area of the operating area, and anesthesia is performed topically with lidocaine, then dermabrasion is performed using a rotary apparatus and the skin surfaces are applied, applying a gauze cloth moistened pre-butol, the surgical wound is treated with butol twice 10-15 ml from a plastic syringe without a needle, and 3-5 minutes after the patient has a burning sensation, the gauze is removed with a forceps, after dermabrasion on the operating surface impose a collagen coating that exceeds the operating area by 1-2 cm, and the surgical wound is treated twice without interruption
  • the objective of the invention is to provide a simpler highly effective method for the treatment of atrophic scars, which allows to obtain a pronounced effect in a short time.
  • the technical result is to reduce the complexity and cost, as well as to shorten the treatment time and improve functional results.
  • the technical result is achieved by a method of treating atrophic skin scars using fully straightened filtered high-frequency radio waves and autologous platelet-rich plasma, characterized in that from the patient’s peripheral vein take blood in vacuum tubes, mix blood with an anticoagulant, centrifuge the blood mixture with an anticoagulant for 4-6 minutes with a centrifuge speed of 700-900 rpm, remove the upper layer of platelet-rich plasma from a tube into a separate tube, activate platelet-rich plasma plasma with calcium ions by adding calcium gluconate and mixing with it, activated platelet-rich plasma is collected in a syringe and injected intradermally into the scar, the injected is heated region using the Surgitron ® radio wave apparatus with Pelleve ® GlideSafe or PelleFirm electrodes up to 41-43 ° C for at least 4 minutes.
  • blood is collected from a patient’s peripheral vein into 3-9 ml vacuum tubes; 3.8% sodium citrate solution is used as an anticoagulant; to mix blood with sodium citrate, the tubes are inverted 4-5 times; the upper layer of the resulting platelet-rich plasma is removed into a separate tube using a laboratory dispenser; calcium gluconate in platelet rich plasma is added at the rate of 50 ⁇ l of calcium gluconate per 1 ml of plasma; for injection use insulin syringes with a volume of 1 ml; 0.1-0.2 ml of platelet-rich plasma per 1 cm of the rumen is injected into the rumen; heating is carried out using a radio wave apparatus
  • the proposed method is as follows. From the patient's peripheral vein, blood is drawn into vacuum tubes of 3–9 ml in volume (depending on the required plasma volume). Blood is mixed with an anticoagulant, which is used as a 3.8% solution of sodium citrate. To mix blood with citrate, the tubes are inverted 4-5 times.
  • the blood in the test tubes is divided into three layers: the lower is red blood cells, the middle is a thin layer of white blood cells, and the upper is plasma enriched with platelets.
  • platelet-rich plasma (BTP) is removed into a separate tube. After this, platelet-rich plasma (BTP) is activated with calcium ions: 50 ⁇ l of calcium gluconate per 1 ml of plasma.
  • BoTP BoTP is ready for use and should be used within 5-10 minutes.
  • the plasma volume required for the procedure depends on the size of the skin defect.
  • BTP All obtained BTP are collected in 1 ml insulin syringes. BOTP is injected intradermally into the scar at 0.1-0.2 ml per cm.
  • the procedure is carried out 1 time per week 5-7 times.
  • the method was applied in clinical conditions in 22 patients with atrophic skin scars aged 20 to 50 years. All patients were female. 12 patients with post-acne without signs of inflammation (atrophic scars resulting from acne). 10 patients with striae (atrophic scars). The disease is over 2 years old. The procedure was performed 1 time per week.
  • Example 1 Patient T., 37 years old.
  • the patient was treated by the proposed method. 12 ml of blood were taken from peripheral blood (in 4 vacuum tubes of 3 ml each). 3.8% sodium citrate solution was used as an anticoagulant. Further, the blood was centrifuged for 5 min with a centrifuge rotation speed of 800 rpm. During rotation, the blood in the test tubes was divided into three layers: the lower — red blood cells, the middle — a thin layer of leukocytes, and the upper — plasma enriched with platelets. Then, using the laboratory dispenser, the upper layer (BOT) was removed into a separate tube.
  • BOTP was activated with calcium ions: 50 ⁇ l of calcium gluconate per 1 ml of plasma. The liquid was mixed by carefully turning the tubes. Received 5 ml of BOTP. All obtained BTPs were collected in 1 ml insulin syringes. BOTP was injected intradermally into the scar at 0.1-0.2 ml per cm. After BOTP injection into the scar, this area was heated using a Surgitron S5 radio wave apparatus with a PelleFirm TM electrode to 42 ° C for 5 minutes (the temperature was measured with an infrared pyrometer on the skin above the scar) to resume regeneration processes. The procedure was carried out 1 time per week 5 times. After 6 months there was a complete regression of striae. By the end of 2 months, a significant densification of scar tissue and alignment of the relief with the surrounding skin were noted. No adverse effects have been reported.
  • platelets due to short-term centrifugation for 4-6 minutes, platelets do not have time to sink to the bottom of the tube and mix with the layer of leukocytes and red blood cells, but remain suspended in the upper part of the tube, evenly distributed in the plasma, which does not require separation of the plasma into layers or repeated centrifugation.
  • the short time required for the manufacture of platelet-rich plasma, and the simplicity of its preparation make it possible to use this technique in routine medical practice in institutions of any profile.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Cell Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Surgery (AREA)
  • Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Epidemiology (AREA)
  • Plasma & Fusion (AREA)
  • Zoology (AREA)
  • Virology (AREA)
  • Otolaryngology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Medical Informatics (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dermatology (AREA)
  • Infusion, Injection, And Reservoir Apparatuses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

L'invention concerne le domaine de la médecine et notamment de la dermatologie et de la cosmétologie et peut être utilisée dans le traitement des cicatrices atrophiques de la peau ayant des origines différentes. Le résultat technique consiste à réduire les besoins en main d'oeuvre et le coût ainsi que la durée du traitement et améliorer les résultats fonctionnels. La méthode de traitement des cicatrices atrophiques de la peau au moyen d'ondes radioélectriques filtrées totalement redressées de fréquence élevée et d'un plasma autologue riche en thrombocytes est caractérisée en ce que l'on prélève du sang d'une veine périphérique du patient dans des éprouvettes sous vide, on mélange le sang avec un anticoagulant et on soumet leur mélange à une centrifugation pendant 4 à 6 min à une vitesse de rotation de la centrifugeuse de 700-900 tours à la minute, après quoi on dépose dans une éprouvette séparée la couche supérieure de plasma riche en thrombocytes et on l'active avec des ions de calcium en y ajoutant du gluconate de calcium et en mélangeant le tout. Le plasma activé par un plasma riche en thrombocytes remplit une seringue et est injecté par procédé intradermique dans la cicatrice puis la région injectée est chauffée avec un appareil à ondes radioélectriques Surgitron® avec des électrodes Pelleve®GlideSafe ou PelleFirm jusqu'à 41-43 °C pendant au moins 4 minutes.
PCT/RU2017/000595 2016-08-18 2017-08-17 Méthode pour traiter les cicatrices atrophiques de la peau Ceased WO2018034595A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
RU2016133902 2016-08-18
RU2016133902A RU2627468C1 (ru) 2016-08-18 2016-08-18 Способ лечения атрофических рубцов кожи

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WO2018034595A1 true WO2018034595A1 (fr) 2018-02-22

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WO (1) WO2018034595A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2692637C1 (ru) * 2018-07-18 2019-06-25 федеральное государственное бюджетное учреждение "Центральный научно-исследовательский институт стоматологии и челюстно-лицевой хирургии" Министерства здравоохранения Российской Федерации Способ устранения атрофических рубцов мягких тканей
RU2733576C1 (ru) * 2020-05-18 2020-10-05 Федеральное государственное бюджетное учреждение "Национальный медицинский исследовательский центр глазных болезней имени Гельмгольца" Министерства здравоохранения Российской Федерации (ФГБУ "НМИЦ ГБ им. Гельмгольца" Минздрава России) Способ лечения посттравматических рубцов периорбитальной области и области век

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8317782B1 (en) * 2006-10-13 2012-11-27 Ellman International, Inc. Non-ablative radio-frequency treatment of skin tissue
WO2013007308A1 (fr) * 2011-07-14 2013-01-17 Garbin Stefania Composition de plasma riche en plaquettes pour la réparation et la régénération tissulaire

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2230554C2 (ru) * 2002-05-07 2004-06-20 Глубокова Ирина Борисовна Способ лечения нормо-атрофических рубцов механической дермабразией с применением комбинированного коллагенового покрытия с антимикробным средством
RU2389516C2 (ru) * 2008-06-23 2010-05-20 Общество С Ограниченной Ответственностью "Новые Энергетические Технологии" Способ радиочастотного двухэтапного ремоделирования атрофической кожи лица и тела

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8317782B1 (en) * 2006-10-13 2012-11-27 Ellman International, Inc. Non-ablative radio-frequency treatment of skin tissue
WO2013007308A1 (fr) * 2011-07-14 2013-01-17 Garbin Stefania Composition de plasma riche en plaquettes pour la réparation et la régénération tissulaire

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
???????????? H..B.: "??????????, ??????? ???????????? nna3Ma, B ??????? ???????? ????????? ???? ?????? ???????????", ??????????? HA ????????? ?????? ??????? ????????? ??????????? ????, M., 2014, pages 64 - 66 *
KIM I S ET AL.: "Efficacy of intradermal radiofrequency combined with autologous platelet-rich plasma in striae distensae: a pilot study", INT J DERMATOL., vol. 51, no. 10, October 2012 (2012-10-01), pages 1253 - 1258, XP055464615 *
RADIOFREQUENCY ENERGY SOURCES & ACCESSORY PRODUCTS, 2013, pages 3, 5, 6, 52, Retrieved from the Internet <URL:http://www.ellman.com/PDF/MedicalProductsCatalog2013.pdf> *

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