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WO1996042015B1 - Reactif et procede de determination differentielle de leucocytes dans le sang - Google Patents

Reactif et procede de determination differentielle de leucocytes dans le sang

Info

Publication number
WO1996042015B1
WO1996042015B1 PCT/US1996/007762 US9607762W WO9642015B1 WO 1996042015 B1 WO1996042015 B1 WO 1996042015B1 US 9607762 W US9607762 W US 9607762W WO 9642015 B1 WO9642015 B1 WO 9642015B1
Authority
WO
WIPO (PCT)
Prior art keywords
reagent
blood
leukocytes
lytic reagent
stabilizing reagent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1996/007762
Other languages
English (en)
Other versions
WO1996042015A2 (fr
WO1996042015A3 (fr
Filing date
Publication date
Priority claimed from US08/488,630 external-priority patent/US5686308A/en
Application filed filed Critical
Priority to DE69603491T priority Critical patent/DE69603491T2/de
Priority to EP96916665A priority patent/EP0846264B1/fr
Priority to JP50307997A priority patent/JP3679127B2/ja
Publication of WO1996042015A2 publication Critical patent/WO1996042015A2/fr
Publication of WO1996042015A3 publication Critical patent/WO1996042015A3/fr
Publication of WO1996042015B1 publication Critical patent/WO1996042015B1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Abstract

L'invention concerne une composition de réaction lytique qui effectue la stromatolyse sélective des globules rouges dans un échantillon sanguin. Elle concerne, de plus, un système réactif lytique permettant la différenciation d'au moins deux sous-populations de leucocytes. Elle concerne également un procédé d'utilisation de ce système réactif lytique, qui permet de déterminer l'hémoglobine dans le sang. Ce système combine un réactif lytique à base d'un composé éthoxylé à chaîne longue avec un acide et un réactif de stabilisation hypertonique alcalin. Ce système et le procédé d'analyse associé maintiennent la morphologie cellulaire des leucocytes, et on peut les utiliser afin d'analyser des échantillons sanguins normaux ou anormaux, du sang frais ou vieilli, des échantillons sanguins de l'homme et de l'animal, ainsi que d'autres échantillons fluides de liquides, tels que la moelle osseuse.
PCT/US1996/007762 1995-06-08 1996-05-24 Reactif et procede de determination differentielle de leucocytes dans le sang Ceased WO1996042015A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
DE69603491T DE69603491T2 (de) 1995-06-08 1996-05-24 Reagenz und verfahren zur differentiellen bestimmung von leukocyten im blut
EP96916665A EP0846264B1 (fr) 1995-06-08 1996-05-24 Reactif et procede de determination differentielle de leucocytes dans le sang
JP50307997A JP3679127B2 (ja) 1995-06-08 1996-05-24 血液中の白血球の鑑別式決定のための試薬及び方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/488,630 US5686308A (en) 1995-06-08 1995-06-08 Reagent and method for differential determination of leukocytes in blood
US08/488,630 1995-06-08

Publications (3)

Publication Number Publication Date
WO1996042015A2 WO1996042015A2 (fr) 1996-12-27
WO1996042015A3 WO1996042015A3 (fr) 1997-05-15
WO1996042015B1 true WO1996042015B1 (fr) 1997-07-17

Family

ID=23940472

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/007762 Ceased WO1996042015A2 (fr) 1995-06-08 1996-05-24 Reactif et procede de determination differentielle de leucocytes dans le sang

Country Status (5)

Country Link
US (1) US5686308A (fr)
EP (1) EP0846264B1 (fr)
JP (1) JP3679127B2 (fr)
DE (1) DE69603491T2 (fr)
WO (1) WO1996042015A2 (fr)

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CN104024437A (zh) * 2011-12-27 2014-09-03 雅培制药有限公司 体液的细胞分析
BR112015011723A2 (pt) * 2012-11-30 2017-07-11 Beckman Coulter Inc triagem de tuberculose com o uso de dados de cpd
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