[go: up one dir, main page]

WO1996042015B1 - Reagent and method for differential determination of leukocytes in blood - Google Patents

Reagent and method for differential determination of leukocytes in blood

Info

Publication number
WO1996042015B1
WO1996042015B1 PCT/US1996/007762 US9607762W WO9642015B1 WO 1996042015 B1 WO1996042015 B1 WO 1996042015B1 US 9607762 W US9607762 W US 9607762W WO 9642015 B1 WO9642015 B1 WO 9642015B1
Authority
WO
WIPO (PCT)
Prior art keywords
reagent
blood
leukocytes
lytic reagent
stabilizing reagent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1996/007762
Other languages
French (fr)
Other versions
WO1996042015A2 (en
WO1996042015A3 (en
Filing date
Publication date
Priority claimed from US08/488,630 external-priority patent/US5686308A/en
Application filed filed Critical
Priority to DE69603491T priority Critical patent/DE69603491T2/en
Priority to EP96916665A priority patent/EP0846264B1/en
Priority to JP50307997A priority patent/JP3679127B2/en
Publication of WO1996042015A2 publication Critical patent/WO1996042015A2/en
Publication of WO1996042015A3 publication Critical patent/WO1996042015A3/en
Publication of WO1996042015B1 publication Critical patent/WO1996042015B1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Abstract

A lytic reagent composition is provided which selectively stromatolyses red blood cells in a blood sample. In addition, a lytic reagent system is provided which enables the differentiation of at least two subpopulations of leukocytes. A method for using the lytic reagent system is also provided. Still further, the lytic reagent system finds use in the determination of the hemoglobin in the blood. The lytic reagent system utilizes an ethoxylated long chain compound based lysis reagent with an acid and a hypertonic, alkaline, stabilizing reagent. The system and analysis method maintains the cellular morphology of the leukocytes and can be used to analyze normal and abnormal blood samples, fresh and aged blood, human and non-human animal blood samples, as well as other fluid samples such as bone marrow.

Claims

AMENDED CLAIMS
[received by the International Bureau on 10 June 1997 (10.06.97); original claim 28 amended; remaining claims unchanged (1 page)]
25. The method of claim 17, wherein the hypertonic alkaline stabilizing reagent composition comprises chloπde salts, sulfate salts and a buffer.
26. The hypertonic alkaline stabilizing reagent of claim 25, wherein the aqueous, hypertonic alkaline stabilizing reagent comprises: a a chloride salt selected from the group consisting of sodium chloride and potassium chloride in an amount of 0.25%- % by weight based on the total weight of the stabilizing reagent; and b a sulfate salt selected from the group consisting of sodium sulfate and potassium sulfate in an amount of 0.25% to 9% by weight based on the total weight of the stabilizing reagent.
27 The lytic reagent system of claim 17, which further comprises a buffer, wherein the pH of said stabilizing reagent is adjusted to a pH of 7 to 12.5 with said buffer
28 A method for differentiation of at least four subpopulations of leukocytes in a blood sample comprising a analyzing a blood sample that has been treated with a lytic reagent of claim 1 to obtain at least four subpopulations of leukocytes, said analysis selected from two members of the group consisting of (1 ) D.C. volume,
(2) RF size,
(3) opacity,
(4) light scatter, and
(5) fluorescence b. reporting the results of such analysis in an instrument
PCT/US1996/007762 1995-06-08 1996-05-24 Reagent and method for differential determination of leukocytes in blood Ceased WO1996042015A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
DE69603491T DE69603491T2 (en) 1995-06-08 1996-05-24 REAGENT AND METHOD FOR DIFFERENTIAL DETERMINATION OF LEUKOCYTES IN BLOOD
EP96916665A EP0846264B1 (en) 1995-06-08 1996-05-24 Reagent and method for differential determination of leukocytes in blood
JP50307997A JP3679127B2 (en) 1995-06-08 1996-05-24 Reagent and method for determining differential formula of leukocytes in blood

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/488,630 US5686308A (en) 1995-06-08 1995-06-08 Reagent and method for differential determination of leukocytes in blood
US08/488,630 1995-06-08

Publications (3)

Publication Number Publication Date
WO1996042015A2 WO1996042015A2 (en) 1996-12-27
WO1996042015A3 WO1996042015A3 (en) 1997-05-15
WO1996042015B1 true WO1996042015B1 (en) 1997-07-17

Family

ID=23940472

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/007762 Ceased WO1996042015A2 (en) 1995-06-08 1996-05-24 Reagent and method for differential determination of leukocytes in blood

Country Status (5)

Country Link
US (1) US5686308A (en)
EP (1) EP0846264B1 (en)
JP (1) JP3679127B2 (en)
DE (1) DE69603491T2 (en)
WO (1) WO1996042015A2 (en)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5834315A (en) * 1994-12-23 1998-11-10 Coulter Corporation Cyanide-free reagent and method for hemoglobin determination and leukocyte differentitation
US5882933A (en) * 1995-06-08 1999-03-16 Coulter International Corp. Method for determination of leukocytes and hemoglobin concentration in blood
US5843608A (en) * 1995-06-08 1998-12-01 Coulter International Corp. Reagent and method for differential determination of leukocytes in blood
US5786224A (en) * 1995-06-29 1998-07-28 Coulter Corporation Reagent and method for differential determination of leukocytes in blood
US5882934A (en) * 1997-01-21 1999-03-16 Coulter International Corp. Composition and method for hemoglobin and cell analysis
US5763280A (en) * 1997-01-21 1998-06-09 Coulter International Corp. Cyanide-free lytic reagent composition and method for hemoglobin and cell analysis
JP3830613B2 (en) 1997-04-18 2006-10-04 シスメックス株式会社 Reagent for measuring leukocyte and hemoglobin concentration in blood
US5917584A (en) * 1997-11-21 1999-06-29 Coulter International Corp. Method for differentiation of nucleated red blood cells
US5874310A (en) * 1997-11-21 1999-02-23 Coulter International Corp. Method for differentiation of nucleated red blood cells
US5874311A (en) * 1997-11-21 1999-02-23 Coulter International Corp. Method for differentiation of reticulocytes in blood
US6214625B1 (en) * 1999-04-28 2001-04-10 Coulter International Corp. Composition and method for differentiation of basophils and eosinophils in blood
US6210969B1 (en) * 1999-04-28 2001-04-03 Coulter International Corp. Composition and method for differentiation of basophil and eosinophil subpopulations of leukocytes in blood
FR2813891B1 (en) * 2000-09-14 2005-01-14 Immunotech Sa MULTIFUNCTIONAL REAGENT FOR ERYTHROCYTES INVOLVING CARBAMATES AND APPLICATIONS
WO2002063367A1 (en) * 2001-02-02 2002-08-15 Cellomics, Inc. Method for estimating the best initial focus position
US6573102B2 (en) * 2001-07-27 2003-06-03 Coulter International Corp. Lytic reagent composition for determination of nucleated blood cells
US6472215B1 (en) 2001-07-27 2002-10-29 Coulter International Corp. Method of analyzing nucleated red blood cells in a blood sample
US6743634B2 (en) * 2002-08-23 2004-06-01 Coulter International Corp. Method and apparatus for differentiating blood cells using back-scatter
US7247484B2 (en) * 2002-11-01 2007-07-24 Streck, Inc. Hematology reagent and methods
US7465584B2 (en) * 2005-01-24 2008-12-16 Sysmex Corporation Method and reagent for classifying leukocytes in animal blood
CN101349644B (en) 2007-07-20 2012-06-27 深圳迈瑞生物医疗电子股份有限公司 Leukocytes classification agent and use method thereof
US8102161B2 (en) * 2007-09-25 2012-01-24 Tdk Corporation Stable output in a switching power supply by smoothing the output of the secondary coil
CN105440725A (en) 2008-01-04 2016-03-30 深圳迈瑞生物医疗电子股份有限公司 Asymmetric cyanine fluorescent dye, composition and application in biological sample dyeing
CN101602762B (en) 2008-06-10 2013-10-16 深圳迈瑞生物医疗电子股份有限公司 Asymmetric cyanine compound, preparation method and application thereof
CN101726579B (en) 2008-10-17 2014-06-18 深圳迈瑞生物医疗电子股份有限公司 Blood test reagent and method
US20100120081A1 (en) * 2008-11-07 2010-05-13 Beckman Coulter, Inc. High sensitivity parameters for the detection of vitamin b12 and/or folate deficiencies and methods of use
US8367358B2 (en) 2008-12-17 2013-02-05 Shenzhen Mindray Bio-Medical Electronics Co., Ltd. Reagent, kit and method for differentiating and counting leukocytes
CN101988082B (en) 2009-07-31 2015-04-08 深圳迈瑞生物医疗电子股份有限公司 Leukocyte classified counting reagent, kit and preparation method thereof and method of leukocyte classified counting
CN104024437A (en) * 2011-12-27 2014-09-03 雅培制药有限公司 Cellular analysis of body fluids
BR112015011723A2 (en) * 2012-11-30 2017-07-11 Beckman Coulter Inc screening for tuberculosis using cpd data
RU2564437C2 (en) * 2013-12-26 2015-09-27 Федеральное государственное бюджетное научное учреждение "Всероссийский научно-исследовательский институт бруцеллеза и туберкулеза животных" (ФГБНУ ВНИИБТЖ) Method of determining general redox activity of phagocytes in nitroblue tetrazolium reduction test for leukaemia of cattle
EP3371591A4 (en) 2015-11-02 2018-11-14 Chiranjit Deka Light scatter based apparatus and methods for hematology analysis using only three detectors
CN110501490B (en) * 2019-09-12 2022-06-10 核工业总医院 Isotonic hemolysin, preparation method thereof, method for treating biological sample and method for detecting leukocyte membrane antigen
WO2022099659A1 (en) * 2020-11-13 2022-05-19 大连理工大学 Leukocyte classification reagent, erythrocyte analysis reagent, reagent kit, and analysis method

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3741875A (en) * 1970-10-30 1973-06-26 Mount Sinai Res Foundation Inc Process and apparatus for obtaining a differential white blood cell count
US4158054A (en) * 1973-10-18 1979-06-12 Duncan Flockhart & Co. Ltd. Preparation of virus sub-unit vaccines
US4185964A (en) * 1977-02-08 1980-01-29 Central Laboratories of Associated Maryland Pathologists, Ltd. Lysing reagent
US4286963A (en) * 1979-11-23 1981-09-01 Coulter Electronics, Inc. Differential lymphoid-myeloid determination of leukocytes in whole blood
DE3004611A1 (en) * 1980-02-08 1981-08-13 Hoechst Ag, 6000 Frankfurt HIGHLY CONCENTRATED FUEL SLURRIES
US4525296A (en) * 1980-10-27 1985-06-25 Petrolite Corporation Halide free corrosion inhibitors
US4528274A (en) * 1982-07-06 1985-07-09 Coulter Electronics, Inc. Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes
US4485175A (en) * 1983-01-03 1984-11-27 Coulter Electronics, Inc. Method for three-volume differential determination of lymphocyte, monocyte and granulocyte populations of leukocytes
JPS62112695A (en) * 1985-11-09 1987-05-23 Yushiro Do Brazil Ind Chem Ltd Cold rolling oil composition
US4968629A (en) * 1986-08-20 1990-11-06 Hematology Marketing Associates, Inc. Blood diluent for automatic and semi-automatic determination of white cells and method of utilizing same
KR970007077B1 (en) * 1987-03-13 1997-05-02 코울터 일렉트로닉스 인커퍼레이티드 Multi-Partial Identification Method Using Light Scattering Technique
US5155044A (en) * 1987-03-13 1992-10-13 Coulter Electronics, Inc. Lysing reagent system for isolation, identification and/or analysis of leukocytes from whole blood samples
US5389549A (en) * 1987-05-29 1995-02-14 Toa Medical Electronics Co., Ltd. Method for classifying leukocytes and a reagent used therefor
JP2619900B2 (en) * 1988-01-27 1997-06-11 東亜医用電子株式会社 Reagent and method for measuring leukocytes and hemoglobin in blood
JP2836865B2 (en) * 1989-10-23 1998-12-14 東亜医用電子株式会社 Reagents for measuring leukocytes and hemoglobin in blood
FR2658300B1 (en) * 1990-02-13 1992-05-29 Abx Sa REAGENT AND METHOD OF USING THE SAME FOR THE AUTOMATIC NUMBERING OF BASOPHILIC BLOOD LEUKOCYTES IN MEASURING DEVICES BY VARIATION OF RESISTIVITY.
US5242832A (en) * 1990-03-01 1993-09-07 Toa Medical Electronics Co., Ltd. Reagent for measurement of leukocytes and hemoglobin in blood
US5198485A (en) * 1991-08-12 1993-03-30 Eastman Kodak Company Aqueous coating composition comprising chlorinated polyolefin

Similar Documents

Publication Publication Date Title
WO1996042015B1 (en) Reagent and method for differential determination of leukocytes in blood
WO1996042015A3 (en) Reagent and method for differential determination of leukocytes in blood
WO1997001757B1 (en) Reagent and method for differential determination of leukocytes in blood
US5786224A (en) Reagent and method for differential determination of leukocytes in blood
US5882933A (en) Method for determination of leukocytes and hemoglobin concentration in blood
DE69127888T2 (en) Preservation solution for cells
WO1999023489B1 (en) Reagent and method for differential determination of leukocytes in blood
US7541190B2 (en) Method of measurement of cellular hemoglobin
US6114130A (en) Reagent for measurement of the haemoglobin and determination of the leukocytes in a blood sample
NZ197422A (en) Determination of lymphoid/myeloid ratio in a leukocyte population
EP2324350B1 (en) Reference control for cell by cell analysis
DE3854326T2 (en) TREATMENT TECHNOLOGY FOR WHOLE BLOOD PATTERN.
Hübl et al. Investigation of the pathogenesis of massive hemolysis in a case of Clostridium perfringens septicemia
Wagener et al. Anaemia in South American camelids–an overview of clinical and laboratory diagnostics
Weiss Primary pure red cell aplasia in dogs: 13 cases (1996–2000)
EP1863348B1 (en) Cell permeabilization and stabilization reagent and method of use
CA2019426A1 (en) Lithium salts as red blood cells lysing hemoglobin denaturing reagents
AU625137B2 (en) Lithium salts as red blood cell lysing and hemoglobin denaturing reagents
US5627213A (en) Preparation for lysing erythrocytes
WO2009017959A1 (en) Method of measurement of micronucleated erythrocyte populations
Morgan et al. Effects of in vitro hemolysis on serum biochemistry values of the bottlenose dolphin (Tursiops truncatus)
Bhardwaj et al. Haematobiochemical profiles of cows shedding acid-fast bacilli vis-à-vis normal cows
CN120213752A (en) A white blood cell classification and counting kit