RU2349327C1 - Method of accelerated thromboplastin formation stabilisation for newborn piglets with anaemia - Google Patents

Abstract

FIELD: medicine, veterinary science.

SUBSTANCE: method involves prescription of intramuscular injection of ferroglucinum by 150 mg (2 ml) to piglets with anaemia twice over 10 day interval, intramuscular injection of phosprenyl thrice by different syringes into different points, by 0.10 ml/kg for the first time together with iron agent, by 0.09 ml/kg over five days after the first injection, by 0.06 ml/kg for the third time together with the second injection. Additionally, 0.015 ml/kg of gamavit is injected intramuscularly once per day for eight days beginning from the first ferroglucinum injection.

EFFECT: possible thromboplastin formation stabilisation for newborn piglets with anaemia.

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Description

The invention relates to biology and veterinary medicine.

Analogues of the proposed method for the normalization of thromboplastin formation in newborn piglets with anemia does not exist.

There is information about the use of ferroglukin in anemia in piglets (Internal non-communicable diseases of farm animals. Edited by V.M. Danilevsky. M: Agropromizdat, 1991, 576 pp.).

As effective stimulants of the vital functions of a growing organism in modern veterinary medicine, phosprenyl is used - a drug based on polyprenol phosphates obtained from needles and gamavit - a complex balanced means based on the placenta containing sodium nucleinate, a set of amino acids, vitamins and salts (Deeva A.V., Salakhova ZA, Lobova TP, et al. Improving the safety and productivity of piglets when using fosprenil and gamavita. // Veterinary medicine. - 2006, - No. 4, - p.13-15). However, never before has a treatment complex consisting of ferroglukin, fosprenil and gamavit been used in newborn piglets with anemia in order to normalize their increased thromboplastin formation.

The aim of the invention is the normalization of thromboplastin formation in newborn piglets with anemia.

The essence of the proposed method is that to normalize thromboplastin formation in newborn piglets with anemia, 150 mg (2 ml) of ferroglukin is administered intramuscularly, twice with an interval of 10 days, phosprenyl intramuscularly, three times in different syringes at different points, the first time is 0.10 ml / kg simultaneously with the iron preparation, a second time at a dose of 0.09 ml / kg five days after the first injection of ferroglucrin, a third time at a dose of 0.06 ml / kg at the same time as a second injection of ferroglukin and gamavit 0.015 ml / kg intramuscularly once a day , eight days after the first injection of ferroglukin.

The method allows to normalize thromboplastin formation in newborn piglets with anemia 5 days after the end of treatment. Subsequently, thromboplastin formation is normal for these piglets, which will significantly improve the number of pigs, normalize weight gain, reduce their pathological burden, reduce mortality and improve the quality of meat products.

The inventive method is as follows.

The formation of endogenous thromboplastin is determined by Biggs R., Douglas A.S., Macparlane R.G. The formation of throm-boplastin in human blood. // J. Physiol. 1953, - vol. 119, No. 1, - p. 89-104.

The principle of the method. The activity and time of formation of thromboplastin from the component of the plates, deprothrombin plasma and serum of the test blood according to the thrombin time of the test plasma in one-minute intervals. When this mixture is incubated with calcium for 3-6 minutes, highly active thromboplastin is formed.

To determine endogenous thromboplastin, the following ingredients are required:

1) platelet suspension;

2) deprothrombin plasma;

3) serum;

4) calcium chloride.

Reagents:

1) 0.1 M sodium oxalate solution;

2) 1/40 M calcium chloride solution;

3) 0.15 M suspension of barium sulfate;

4) saline solution.

In a silicone tube that contains 0.6 ml of a 0.1 M sodium oxalate solution, 5.4 ml of blood is drawn from a vein with a dry needle. Carefully mix the contents of the tube. At the same time, 1-2 ml of blood is taken into another tube, from which serum is then obtained.

Preparation of a platelet suspension: oxalate blood in a silicone tube is centrifuged for 5-7 minutes at 1500 rpm. The plasma is aspirated with a silicone pipette into another silicone or plastic tube. Platelet-rich plasma is centrifuged for 30 minutes at 3,000 rpm. Platelets settle to the bottom of the tube. A plasma that contains virtually no platelets is aspirated into a separate tube, and physiological saline is added to the platelets, carefully mixed and centrifuged again for 30 minutes at 3000 rpm. Saline is aspirated and the platelet washing procedure is repeated again. After washing the platelets twice, physiological saline is added to the pellet in volume equal to 1/3 of the amount of oxalate plasma taken for centrifugation. The contents are well mixed, getting a working suspension of platelets.

Preparation of a solution of deprothrombin plasma: 0.5-1 ml of platelet-free plasma, which was collected after centrifugation for 30 minutes at 3000 rpm, is deprothrombinized with a barium sulfate suspension, as described above. Deprothrombin plasma is diluted with saline in a ratio of 1: 4.

Preparation of serum solution: 1-2 ml of venous blood taken in a dry test tube, put in a water bath at 37 ° C for 2 hours. Serum is separated from the blood clot by centrifugation and diluted with saline in a ratio of 1: 8.

Test procedure: 0.1 ml of platelet-free plasma, which remained after centrifugation for 30 minutes at 3000 rpm (a part of this plasma was taken) was added to a series of agglutination tubes (7-8) in a water bath at 37 ° C deprothrombinization). Separately, 0.3 ml of a platelet suspension, 0.3 ml of a solution of deprothrombinized plasma, 0.3 ml of a solution of serum, 0.3 ml of a 1/40 M solution of calcium chloride are poured into an empty tube, also in a water bath, and the stopwatch is turned on. After every minute, 0.1 ml of this mixture and 0.1 ml of a 1/40 M calcium chloride solution are simultaneously introduced into one of a number of tubes, which contains platelet-free plasma, and another stopwatch is switched on. Shaking the tube, note the coagulation time of the plasma. The minute study is continued until the thrombin time of the plasma is as short as possible. This time characterizes the activity of thromboplastin, and the minute at which the mixture coagulated is the time of thromboplastin formation. Normally, thromboplastin is formed at 2-4 minutes, and its activity is 12-14 seconds.

When registering the acceleration of the formation and / or increased activity of thromboplastin in newborn piglets with anemia, the proposed treatment complex consisting of 150 mg ferroglukin (2 ml) intramuscularly, twice with an interval of 10 days, fosprenyl intramuscularly, three times in different syringes at different points, for the first time 0.10 ml / kg simultaneously with the iron preparation, a second time at a dose of 0.09 ml / kg five days after the first injection of ferroglukin, a third time at a dose of 0.06 ml / kg simultaneously with a second injection of ferroglukin and gamavit 0.015 ml / kg intramuscularly Dean once a day, eight days after the first injection ferroglyukina.

This complex normalizes thromboplastin formation 5 days after completion of treatment, which is the basis for improving the herd, reducing the mortality rate and guaranteeing an increase in the quality of meat products.

Example. In a newborn piglet No. 6 with anemia, a violation of erythropoiesis and signs of a decrease in the level of iron in its body (serum iron 12.2 μmol / l, siderocytes 1.2%, with a hemoglobin content of 82.6 g / l, erythrocytes - 4.01 × 10 ¹² / l.

In the morning, blood was drawn from a vein and the time of formation and activity of thromboplastin were determined, which amounted to 1.3 min and 10.0 s, respectively. A piglet was prescribed ferroglukin 150 mg intramuscularly, twice with an interval of 10 days, phosprenyl intramuscularly, three times in different syringes at different points, the first time 0.10 ml / kg simultaneously with the iron preparation, the second time at a dose of 0.09 ml / kg five days after the first injection of ferroglukin, the third time at a dose of 0.06 ml / kg simultaneously with the second injection of ferroglukin and gamavit 0.015 ml / kg intramuscularly once a day, eight days, starting with the first injection of ferroglukin.

5 days after the end of treatment, the pig achieved normalization of red blood - serum iron 19.9 μmol / L, siderocytes 8.5%, with hemoglobin 125.0 g / L, erythrocytes - 5.11 × 10 ¹² / L ) The activity and time of thromboplastin formation normalized, amounting to 12 s and 2.9 min, respectively. Subsequently, the achieved performance in the piglet did not experience negative dynamics.

Using the proposed method of normalizing thromboplastin formation in biology and veterinary medicine will help to improve the herd of pigs, increase their weight gain, reduce mortality and subsequently receive healthy offspring from these animals.

Claims (1)

A method for accelerated normalization of thromboplastin formation in newborn piglets with anemia, including the administration of 150 mg of ferroglukin intramuscularly, twice with an interval of 10 days, fosprenil intramuscularly, three times in different syringes at different points, the first time 0.10 ml / kg simultaneously with the drug iron, a second time at a dose of 0.09 ml / kg five days after the first injection of ferroglukin, a third time at a dose of 0.06 ml / kg simultaneously with a second injection of ferroglukin and hamavit 0.015 ml / kg intramuscularly once a day, eight days, starting from the first ferroglukin injections.