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RU2018108847A - Дифференцировка эмбриональных стволовых клеток человека в панкреатические эндокринные клетки - Google Patents

Дифференцировка эмбриональных стволовых клеток человека в панкреатические эндокринные клетки Download PDF

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RU2018108847A
RU2018108847A RU2018108847A RU2018108847A RU2018108847A RU 2018108847 A RU2018108847 A RU 2018108847A RU 2018108847 A RU2018108847 A RU 2018108847A RU 2018108847 A RU2018108847 A RU 2018108847A RU 2018108847 A RU2018108847 A RU 2018108847A
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cells
differentiation
pancreatic
stem cells
pluripotent stem
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RU2018108847A
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RU2018108847A3 (ru
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Алиреза РЕЗАНИА
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Янссен Байотек, Инк.
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Publication of RU2018108847A publication Critical patent/RU2018108847A/ru

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/50—Cell markers; Cell surface determinants
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/998—Proteins not provided for elsewhere
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

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Claims (16)

1. Способ индукции формирования эндокринных кластеров, включающий культивирование панкреатических эндокринных клеток с агонистом рецептора сфингозина-1.
2. Способ по п.1, в котором для обработки панкреатических эндокринных клеток в качестве агониста рецептора сфингозина-1 используют сфингозин-1-фосфат (S1P).
3. Способ по п.1, в котором панкреатические эндокринные клетки получают путем поэтапной дифференцировки плюрипотентных стволовых клеток.
4. Способ по п.3, в котором плюрипотентные стволовые клетки представляют собой плюрипотентные стволовые клетки человека.
5. Способ по п.1, где способ дополнительно содержит:
дифференцировку плюрипотентных стволовых клеток в конечные клетки энтодермы;
дифференцировку конечных клеток энтодермы в клетки первичной кишечной трубки;
дифференцировку клеток первичной кишечной трубки в клетки передней кишки;
дифференцировку клеток передней кишки в клетки-предшественники панкреатических клеток;
дифференцировку клеток-предшественников панкреатических клеток в клетки панкреатической энтодермы; и
дифференцировку клеток панкреатической энтодермы в панкреатические эндокринные клетки.
6. Способ по п.5, где плюрипотентные стволовые клетки представляют собой плюрипотентные стволовые клетки человека.
7. Способ по п.5, где способ дополнительно включает обработку клеток панкреатической энтодермы эфриновым лигандом.
8. Способ по п.7, где эфриновый лиганд представляет собой эфрин А3 или эфрин А4.
9. Способ по п.5, где способ дополнительно включает обработку клеток панкреатической энтодермы активином А или активином С.
10. Способ по п.5, где способ дополнительно включает обработку клеток панкреатической энтодермы семафорином 3А или эпигеном.
RU2018108847A 2012-06-08 2013-06-06 Дифференцировка эмбриональных стволовых клеток человека в панкреатические эндокринные клетки RU2018108847A (ru)

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US201261657160P 2012-06-08 2012-06-08
US61/657,160 2012-06-08

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RU2018108847A true RU2018108847A (ru) 2019-02-26

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RU2014153529A RU2650813C2 (ru) 2012-06-08 2013-06-06 Использование лигандов эпинефрина для дифференцирования клеток панкреатической эндодермы
RU2018108851A RU2018108851A (ru) 2012-06-08 2013-06-06 Дифференцировка эмбриональных стволовых клеток человека в панкреатические эндокринные клетки
RU2018108847A RU2018108847A (ru) 2012-06-08 2013-06-06 Дифференцировка эмбриональных стволовых клеток человека в панкреатические эндокринные клетки
RU2018108850A RU2018108850A (ru) 2012-06-08 2013-06-06 Дифференцировка эмбриональных стволовых клеток человека в панкреатические эндокринные клетки

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RU2018108851A RU2018108851A (ru) 2012-06-08 2013-06-06 Дифференцировка эмбриональных стволовых клеток человека в панкреатические эндокринные клетки

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EP (5) EP3450544A1 (ru)
JP (3) JP6469003B2 (ru)
KR (4) KR102468315B1 (ru)
CN (4) CN108103006A (ru)
AR (1) AR091388A1 (ru)
AU (4) AU2013271581B2 (ru)
BR (1) BR112014030682A2 (ru)
CA (2) CA3173122A1 (ru)
DK (1) DK3450542T3 (ru)
ES (2) ES2897649T3 (ru)
IN (1) IN2014DN10021A (ru)
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PH (4) PH12014502661A1 (ru)
PL (1) PL2859091T3 (ru)
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