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FI20096288A0 - Formulations and methods for culturing stem cells - Google Patents

Formulations and methods for culturing stem cells

Info

Publication number
FI20096288A0
FI20096288A0 FI20096288A FI20096288A FI20096288A0 FI 20096288 A0 FI20096288 A0 FI 20096288A0 FI 20096288 A FI20096288 A FI 20096288A FI 20096288 A FI20096288 A FI 20096288A FI 20096288 A0 FI20096288 A0 FI 20096288A0
Authority
FI
Finland
Prior art keywords
formulations
methods
stem cells
culturing stem
culturing
Prior art date
Application number
FI20096288A
Other languages
English (en)
Swedish (sv)
Inventor
Kristiina Rajala
Marjo-Riitta Suuronen
Outi Hovatta
Heli Skottman
Original Assignee
Kristiina Rajala
Marjo Riitta Suuronen
Outi Hovatta
Heli Skottman
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kristiina Rajala, Marjo Riitta Suuronen, Outi Hovatta, Heli Skottman filed Critical Kristiina Rajala
Priority to FI20096288A priority Critical patent/FI20096288A0/fi
Publication of FI20096288A0 publication Critical patent/FI20096288A0/fi
Priority to AU2010326512A priority patent/AU2010326512A1/en
Priority to KR1020127017428A priority patent/KR20130009943A/ko
Priority to CA2782296A priority patent/CA2782296A1/en
Priority to JP2012541546A priority patent/JP2013512667A/ja
Priority to PCT/FI2010/050980 priority patent/WO2011067465A1/en
Priority to EP10800970A priority patent/EP2507360A1/en
Priority to CN2010800545573A priority patent/CN102906247A/zh

Links

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/98—Xeno-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Rheumatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
FI20096288A 2009-12-04 2009-12-04 Formulations and methods for culturing stem cells FI20096288A0 (fi)

Priority Applications (8)

Application Number Priority Date Filing Date Title
FI20096288A FI20096288A0 (fi) 2009-12-04 2009-12-04 Formulations and methods for culturing stem cells
AU2010326512A AU2010326512A1 (en) 2009-12-04 2010-11-30 Formulations and methods for culturing stem cells
KR1020127017428A KR20130009943A (ko) 2009-12-04 2010-11-30 줄기세포 배양을 위한 제형 및 방법
CA2782296A CA2782296A1 (en) 2009-12-04 2010-11-30 Formulations and methods for culturing stem cells
JP2012541546A JP2013512667A (ja) 2009-12-04 2010-11-30 幹細胞を培養するための処方物および方法
PCT/FI2010/050980 WO2011067465A1 (en) 2009-12-04 2010-11-30 Formulations and methods for culturing stem cells
EP10800970A EP2507360A1 (en) 2009-12-04 2010-11-30 Formulations and methods for culturing stem cells
CN2010800545573A CN102906247A (zh) 2009-12-04 2010-11-30 用于培养干细胞的制剂和方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FI20096288A FI20096288A0 (fi) 2009-12-04 2009-12-04 Formulations and methods for culturing stem cells

Publications (1)

Publication Number Publication Date
FI20096288A0 true FI20096288A0 (fi) 2009-12-04

Family

ID=41462754

Family Applications (1)

Application Number Title Priority Date Filing Date
FI20096288A FI20096288A0 (fi) 2009-12-04 2009-12-04 Formulations and methods for culturing stem cells

Country Status (8)

Country Link
EP (1) EP2507360A1 (fi)
JP (1) JP2013512667A (fi)
KR (1) KR20130009943A (fi)
CN (1) CN102906247A (fi)
AU (1) AU2010326512A1 (fi)
CA (1) CA2782296A1 (fi)
FI (1) FI20096288A0 (fi)
WO (1) WO2011067465A1 (fi)

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CA3207103A1 (en) 2007-07-31 2009-02-05 Janssen Biotech, Inc. Differentiation of human embryonic stem cells to pancreatic endocrine
ATE523585T1 (de) 2007-11-27 2011-09-15 Lifescan Inc Differenzierung menschlicher embryonaler stammzellen
RU2551772C2 (ru) 2008-02-21 2015-05-27 Сентокор Орто Байотек Инк. Способы, поверхностно-модифицированные носители и композиции для иммобилизации, культивирования и открепления клеток
BRPI0914116A2 (pt) 2008-06-30 2019-09-17 Centocor Ortho Biotech Inc diferenciação de células-tronco pluripotentes
RU2522001C2 (ru) 2008-10-31 2014-07-10 Сентокор Орто Байотек Инк. Дифференцирование человеческих эмбриональных стволовых клеток в линию панкреатических эндокринных клеток
CN107435038B (zh) 2008-10-31 2021-07-09 詹森生物科技公司 人胚胎干细胞向胰腺内分泌谱系的分化
CA2744227C (en) 2008-11-20 2018-10-02 Centocor Ortho Biotech Inc. Methods and compositions for cell attachment and cultivation on planar substrates
JP2012509085A (ja) 2008-11-20 2012-04-19 ヤンセン バイオテツク，インコーポレーテツド マイクロキャリア上での多能性幹細胞の培養
SG177481A1 (en) 2009-07-20 2012-02-28 Janssen Biotech Inc Differentiation of human embryonic stem cells
CA2784415C (en) 2009-12-23 2019-06-18 Jean Xu Differentiation of human embryonic stem cells
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CN103221536B (zh) 2010-08-31 2016-08-31 詹森生物科技公司 人胚胎干细胞的分化
ES2658146T3 (es) 2010-08-31 2018-03-08 Janssen Biotech, Inc. Diferenciación de células madre embrionarias humanas
JP6148429B2 (ja) * 2011-01-31 2017-06-14 協和発酵バイオ株式会社 ヒト多能性幹細胞の培養方法
AU2012355698B2 (en) 2011-12-22 2018-11-29 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into single hormonal insulin positive cells
US9434920B2 (en) 2012-03-07 2016-09-06 Janssen Biotech, Inc. Defined media for expansion and maintenance of pluripotent stem cells
MX358590B (es) 2012-06-08 2018-08-24 Janssen Biotech Inc Diferenciacion de celulas madre embrionarias humanas en celulas endocrinas pancreaticas.
US10344264B2 (en) 2012-12-31 2019-07-09 Janssen Biotech, Inc. Culturing of human embryonic stem cells at the air-liquid interface for differentiation into pancreatic endocrine cells
AU2013370228B2 (en) 2012-12-31 2018-10-18 Janssen Biotech, Inc. Suspension and clustering of human pluripotent cells for differentiation into pancreatic endocrine cells
SG10201707811XA (en) 2012-12-31 2017-11-29 Janssen Biotech Inc Differentiation of human embryonic stem cells into pancreatic endocrine cells using hb9 regulators
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
EP3006558B1 (en) * 2013-05-30 2020-05-13 Ajinomoto Co., Inc. Medium for culturing stem cells
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JP7154586B2 (ja) * 2016-02-23 2022-10-18 ユニバーシティ－インダストリー コーオペレイション グループ オブ キョンヒ ユニバーシティ 幹細胞の効能改善のための組成物及び方法
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CN110799639A (zh) * 2017-06-27 2020-02-14 味之素株式会社 含有核黄素衍生物的培养基
CA3078759A1 (en) * 2017-10-13 2019-04-18 Boehringer Ingelheim International Gmbh Perfusion medium
JP2020162536A (ja) * 2019-03-29 2020-10-08 興人ライフサイエンス株式会社 組み換えタンパク質産生増加のための培地用組成物
CN114787341B (zh) * 2019-12-09 2024-08-16 株式会社大熊制药 从人多能干细胞制备间充质干细胞的方法及由此制备的间充质干细胞
JP2022023684A (ja) * 2020-07-27 2022-02-08 学校法人日本大学 イヌ間葉系幹細胞培養用培地、イヌ間葉系幹細胞の培養方法及びイヌ間葉系幹細胞の製造方法
WO2023286772A1 (ja) * 2021-07-12 2023-01-19 三菱ケミカル株式会社 心筋細胞の製造方法
CN113832097B (zh) * 2021-09-07 2024-02-20 生物岛实验室 组合物及含有其的无血清、无饲养层干细胞培养基及应用
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Also Published As

Publication number Publication date
WO2011067465A1 (en) 2011-06-09
AU2010326512A1 (en) 2012-06-28
CN102906247A (zh) 2013-01-30
JP2013512667A (ja) 2013-04-18
CA2782296A1 (en) 2011-06-09
KR20130009943A (ko) 2013-01-24
EP2507360A1 (en) 2012-10-10

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