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ES2571446T3 - ADN polimerasas quiméricas - Google Patents

ADN polimerasas quiméricas

Info

Publication number
ES2571446T3
ES2571446T3 ES09829689T ES09829689T ES2571446T3 ES 2571446 T3 ES2571446 T3 ES 2571446T3 ES 09829689 T ES09829689 T ES 09829689T ES 09829689 T ES09829689 T ES 09829689T ES 2571446 T3 ES2571446 T3 ES 2571446T3
Authority
ES
Spain
Prior art keywords
chimeric dna
domain
dna polymerases
polymerase
palm
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES09829689T
Other languages
English (en)
Inventor
Bjarne Faurholm
Paul Mcewan
William Bourn
Gavin Rush
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kapa Biosystems Inc
Original Assignee
Kapa Biosystems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kapa Biosystems Inc filed Critical Kapa Biosystems Inc
Application granted granted Critical
Publication of ES2571446T3 publication Critical patent/ES2571446T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Una ADN polimerasa quimérica que comprende la secuencia aminoacídica de SEQ ID NO: 16, que contiene el dominio N-terminal, el dominio 3'-5' exonucleasa y el dominio de pulgar de polimerasa KOD y el dominio de la palma y los dedos de polimerasa Pfu.
ES09829689T 2008-11-03 2009-11-03 ADN polimerasas quiméricas Active ES2571446T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11086208P 2008-11-03 2008-11-03
PCT/US2009/063166 WO2010062776A2 (en) 2008-11-03 2009-11-03 Chimeric dna polymerases

Publications (1)

Publication Number Publication Date
ES2571446T3 true ES2571446T3 (es) 2016-05-25

Family

ID=42226349

Family Applications (2)

Application Number Title Priority Date Filing Date
ES09829689T Active ES2571446T3 (es) 2008-11-03 2009-11-03 ADN polimerasas quiméricas
ES15160891T Active ES2716402T3 (es) 2008-11-03 2009-11-03 ADN polimerasas quiméricas

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES15160891T Active ES2716402T3 (es) 2008-11-03 2009-11-03 ADN polimerasas quiméricas

Country Status (7)

Country Link
US (2) US9023633B2 (es)
EP (2) EP2927317B1 (es)
JP (3) JP2012507986A (es)
CN (1) CN102257136A (es)
CA (1) CA2742593C (es)
ES (2) ES2571446T3 (es)
WO (1) WO2010062776A2 (es)

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CN104250641B (zh) * 2013-06-28 2017-09-01 北京福安华生物科技有限公司 一种高保真dna聚合酶及其制备和应用
JP6428997B2 (ja) * 2013-08-06 2018-11-28 東洋紡株式会社 核酸増幅法
ES2788949T3 (es) * 2014-06-27 2020-10-23 Illumina Inc Polimerasas modificadas para la incorporación mejorada de análogos de nucleótidos
US9963687B2 (en) 2014-08-27 2018-05-08 New England Biolabs, Inc. Fusion polymerase and method for using the same
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WO2016077795A1 (en) * 2014-11-14 2016-05-19 Illumina, Inc. Polymerases
JP6619152B2 (ja) * 2015-05-08 2019-12-11 国立研究開発法人産業技術総合研究所 フラビンアデニンジヌクレオチド依存型グルコース脱水素酵素活性を有するキメラタンパク質
CN106867978A (zh) * 2015-12-10 2017-06-20 江苏众红生物工程创药研究院有限公司 一种重组高保真dna聚合酶及其编码基因和表达方法
JP6969089B2 (ja) * 2015-12-11 2021-11-24 東洋紡株式会社 核酸増幅用組成物及び核酸増幅法
JP6980375B2 (ja) * 2015-12-11 2021-12-15 東洋紡株式会社 核酸増幅用試薬及び核酸増幅法
LT3402880T (lt) 2016-01-15 2024-03-12 Thermo Fisher Scientific Baltics Uab Termofiliniai dnr polimerazės mutantai
US10323243B2 (en) 2016-01-19 2019-06-18 Board Of Regents, The University Of Texas System Thermostable reverse transcriptase
ES2929367T3 (es) 2016-05-18 2022-11-28 Hoffmann La Roche Amplificación por PCR ultrarrápida cuantitativa usando un dispositivo basado en electrohumectación
FR3052462A1 (fr) * 2016-06-14 2017-12-15 Dna Script Variants d'une adn polymerase de la famille polx
CN106011100A (zh) * 2016-08-08 2016-10-12 吴江近岸蛋白质科技有限公司 一种Pfu DNA聚合酶及其制备方法
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CN106754816B (zh) * 2017-02-24 2020-10-27 依科赛生物科技（太仓）有限公司 一种高保真快速扩增融合酶及其制备方法
US11618891B2 (en) 2017-06-26 2023-04-04 Thermo Fisher Scientific Baltics Uab Thermophilic DNA polymerase mutants
KR20200023454A (ko) 2017-06-30 2020-03-04 코덱시스, 인코포레이티드 T7 rna 폴리머라제 변이체
CA3066642A1 (en) 2017-06-30 2019-01-03 Codexis, Inc. T7 rna polymerase variants
WO2019013451A1 (ko) * 2017-07-12 2019-01-17 주식회사 진캐스트 유전자 변이 특이성이 증가된 dna 중합효소 및 이의 활성 증가용 pcr 버퍼 조성물
WO2019084538A1 (en) 2017-10-27 2019-05-02 Board Of Regents, The University Of Texas System TUMOR SPECIFIC ANTIBODIES, T CELL RECEPTORS AND METHODS OF IDENTIFICATION THEREOF
WO2019143622A1 (en) 2018-01-19 2019-07-25 Bio-Rad Laboratories, Inc. Mutant dna polymerases
EP3862427A4 (en) * 2018-09-03 2022-04-27 BGI Shenzhen RECOMBINANT KOD POLYMERASE
CN112673098B (zh) * 2018-10-31 2023-01-06 亿明达股份有限公司 聚合酶、组合物及使用方法
WO2020117968A2 (en) 2018-12-05 2020-06-11 Illumina, Inc. Polymerases, compositions, and methods of use
CN109628424B (zh) * 2018-12-31 2022-04-26 苏州近岸蛋白质科技股份有限公司 一种新型嵌合dna聚合酶及其制备方法
ES2980651T3 (es) * 2019-01-09 2024-10-02 3Cr Bioscience Ltd Método de detección de una secuencia de ácido nucleico
GB2587177A (en) * 2019-01-09 2021-03-24 3Cr Bioscience Ltd Polymerase
AU2020302099A1 (en) 2019-06-28 2022-01-27 Quantum-Si Incorporated Polymerizing enzymes for sequencing reactions
US20240200040A1 (en) * 2019-12-23 2024-06-20 Bgi Shenzhen Chimeric dna polymerase and application thereof
US20230086471A1 (en) * 2020-02-15 2023-03-23 Agency For Science, Technology And Research A method to amplify a nucleic acid
CN113604450B (zh) * 2021-08-18 2023-08-18 华南理工大学 一种kod dna聚合酶突变体及其制备方法与应用
CN113755465A (zh) * 2021-09-23 2021-12-07 武汉爱博泰克生物科技有限公司 嵌合体dna聚合酶及其制备方法
EP4435098A4 (en) * 2021-11-15 2025-10-01 Bgi Shenzhen CHIMERIC DNA POLYMERASE AND ITS USE
CN119736271A (zh) * 2024-12-27 2025-04-01 安序源生物科技(深圳)有限公司 一种phi29 DNA聚合酶突变体及其用途

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Also Published As

Publication number Publication date
US20120115188A1 (en) 2012-05-10
HK1216032A1 (en) 2016-10-07
US20140363848A1 (en) 2014-12-11
JP2015154787A (ja) 2015-08-27
JP2017029172A (ja) 2017-02-09
US9023633B2 (en) 2015-05-05
EP2352818B1 (en) 2016-02-10
EP2927317A1 (en) 2015-10-07
CA2742593C (en) 2020-05-05
JP2012507986A (ja) 2012-04-05
WO2010062776A3 (en) 2010-08-19
WO2010062776A2 (en) 2010-06-03
CA2742593A1 (en) 2010-06-03
US9388396B2 (en) 2016-07-12
CN102257136A (zh) 2011-11-23
EP2352818A4 (en) 2011-10-12
JP6150847B2 (ja) 2017-06-21
EP2352818A2 (en) 2011-08-10
ES2716402T3 (es) 2019-06-12
EP2927317B1 (en) 2018-12-19

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