[go: up one dir, main page]

DK3488002T3 - Enkeltcelle-helgenombiblioteker og kombinatoriske indekseringsfremgangsmåder til fremstilling deraf - Google Patents

Enkeltcelle-helgenombiblioteker og kombinatoriske indekseringsfremgangsmåder til fremstilling deraf Download PDF

Info

Publication number
DK3488002T3
DK3488002T3 DK17755575.2T DK17755575T DK3488002T3 DK 3488002 T3 DK3488002 T3 DK 3488002T3 DK 17755575 T DK17755575 T DK 17755575T DK 3488002 T3 DK3488002 T3 DK 3488002T3
Authority
DK
Denmark
Prior art keywords
combinatory
weekend
libraries
preparing
single cell
Prior art date
Application number
DK17755575.2T
Other languages
English (en)
Inventor
Andrew C Adey
Frank J Steemers
Sarah A Vitak
Original Assignee
Univ Oregon Health & Science
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Oregon Health & Science, Illumina Inc filed Critical Univ Oregon Health & Science
Application granted granted Critical
Publication of DK3488002T3 publication Critical patent/DK3488002T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1065Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • C40B40/06Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06Biochemical methods, e.g. using enzymes or whole viable microorganisms

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DK17755575.2T 2016-07-22 2017-07-21 Enkeltcelle-helgenombiblioteker og kombinatoriske indekseringsfremgangsmåder til fremstilling deraf DK3488002T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662365916P 2016-07-22 2016-07-22
US201762451305P 2017-01-27 2017-01-27
PCT/US2017/043381 WO2018018008A1 (en) 2016-07-22 2017-07-21 Single cell whole genome libraries and combinatorial indexing methods of making thereof

Publications (1)

Publication Number Publication Date
DK3488002T3 true DK3488002T3 (da) 2021-06-21

Family

ID=60988232

Family Applications (1)

Application Number Title Priority Date Filing Date
DK17755575.2T DK3488002T3 (da) 2016-07-22 2017-07-21 Enkeltcelle-helgenombiblioteker og kombinatoriske indekseringsfremgangsmåder til fremstilling deraf

Country Status (12)

Country Link
US (2) US11535883B2 (da)
EP (2) EP3488002B1 (da)
JP (2) JP7155021B2 (da)
KR (2) KR102475710B1 (da)
CN (2) CN117822128A (da)
AU (3) AU2017299803B2 (da)
CA (1) CA3026773C (da)
DK (1) DK3488002T3 (da)
ES (1) ES2873723T3 (da)
IL (1) IL263920B2 (da)
SG (1) SG11201811504PA (da)
WO (1) WO2018018008A1 (da)

Families Citing this family (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
CN103748236B (zh) 2011-04-15 2018-12-25 约翰·霍普金斯大学 安全测序系统
AU2013338393C1 (en) 2012-10-29 2024-07-25 The Johns Hopkins University Papanicolaou test for ovarian and endometrial cancers
WO2017027653A1 (en) 2015-08-11 2017-02-16 The Johns Hopkins University Assaying ovarian cyst fluid
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
DK3596216T3 (da) 2017-03-13 2022-07-25 Gigagen Inc Systemer og fremgangsmåder til massiv parallel kombinatorisk analyse af enkeltceller
KR102790050B1 (ko) 2017-06-05 2025-04-04 백톤 디킨슨 앤드 컴퍼니 단일 세포를 위한 샘플 인덱싱
CN111868260B (zh) 2017-08-07 2025-02-21 约翰斯霍普金斯大学 用于评估和治疗癌症的方法和材料
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
EP3788171B1 (en) 2018-05-03 2023-04-05 Becton, Dickinson and Company High throughput multiomics sample analysis
US11981891B2 (en) 2018-05-17 2024-05-14 Illumina, Inc. High-throughput single-cell sequencing with reduced amplification bias
KR102507415B1 (ko) * 2018-06-04 2023-03-07 일루미나, 인코포레이티드 고속 대량 단일 세포 전사체 라이브러리 그리고 이의 제조 및 사용 방법
EP3830262B1 (en) * 2018-08-03 2024-08-28 Becton Dickinson and Company Nuclei barcoding and capture in single cells
EP3841219A4 (en) 2018-08-24 2022-05-04 Swift Biosciences, Inc. ASYMMETRIC TARGETED AMPLIFICATION METHODS
US10876148B2 (en) * 2018-11-14 2020-12-29 Element Biosciences, Inc. De novo surface preparation and uses thereof
US10704094B1 (en) 2018-11-14 2020-07-07 Element Biosciences, Inc. Multipart reagents having increased avidity for polymerase binding
GB2613480B (en) 2018-11-15 2023-11-22 Element Biosciences Inc Methods for generating circular nucleic acid molecules
EP3891305A1 (en) 2018-12-05 2021-10-13 Illumina Cambridge Limited Methods and compositions for cluster generation by bridge amplification
SG11202012807YA (en) 2018-12-18 2021-01-28 Illumina Cambridge Ltd Methods and compositions for paired end sequencing using a single surface primer
ES2965222T3 (es) 2018-12-19 2024-04-11 Illumina Inc Métodos para mejorar la prioridad de clonalidad de agrupamientos de polinucleótidos
EP3924506A1 (en) 2019-02-14 2021-12-22 Becton Dickinson and Company Hybrid targeted and whole transcriptome amplification
MX2021003746A (es) 2019-03-01 2021-06-23 Illumina Inc Genotecas de núcleos únicos y células únicas de alto rendimiento y métodos para elaborarlas y usarlas.
US20220205035A1 (en) 2019-04-05 2022-06-30 Board Of Regents, The University Of Texas System Methods and applications for cell barcoding
CA3113272A1 (en) 2019-04-29 2020-11-05 Illumina, Inc. Identification and analysis of microbial samples by rapid incubation and nucleic acid enrichment
US11535891B2 (en) 2019-05-03 2022-12-27 The Regents Of The University Of California Barcoded solid supports and methods of making and using same
EP3980537A4 (en) * 2019-06-04 2023-11-22 Universal Sequencing Technology Corporation Methods of barcoding nucleic acid for detection and sequencing
EP3997217A4 (en) * 2019-07-12 2023-06-28 New York Genome Center, Inc. Methods and compositions for scalable pooled rna screens with single cell chromatin accessibility profiling
CN114051534B (zh) 2019-07-22 2025-02-21 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
GB2589869A (en) * 2019-12-09 2021-06-16 Univ Oxford Innovation Ltd Method for whole genome sequencing of picogram quantities of DNA
CN110952147B (zh) * 2019-12-18 2023-05-05 南方科技大学 单细胞基因组测序用的dna文库的构建方法
BR112021019640A2 (pt) * 2019-12-19 2022-06-21 Illumina Inc Bibliotecas de células únicas de alto rendimento e métodos de preparo e uso
EP4471155A3 (en) 2020-01-29 2024-12-18 Becton, Dickinson and Company Barcoded wells for spatial mapping of single cells through sequencing
CA3170345A1 (en) 2020-02-14 2021-08-19 The Johns Hopkins University Methods and materials for assessing nucleic acids
WO2021168097A1 (en) * 2020-02-19 2021-08-26 Genapsys, Inc. Methods and systems for nucleic acid processing
US12153043B2 (en) 2020-02-25 2024-11-26 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
EP4121552A4 (en) * 2020-03-18 2024-04-03 CZ Biohub SF, LLC Single-cell combinatorial indexed cytometry sequencing
JP2023518250A (ja) * 2020-03-20 2023-04-28 ミッション バイオ インコーポレイテッド 全ゲノム増幅のためのシングルセルワークフロー
EP4150118A1 (en) 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
IL298821A (en) * 2020-06-09 2023-02-01 Illumina Inc Methods for increasing yield of sequencing libraries
WO2021252617A1 (en) 2020-06-09 2021-12-16 Illumina, Inc. Methods for increasing yield of sequencing libraries
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
CN111996598A (zh) * 2020-08-11 2020-11-27 杭州瀚因生命科技有限公司 一种单细胞染色质可及性的建库方法
CA3187250A1 (en) * 2020-08-14 2022-02-17 John Daniel WELLS In situ library preparation for sequencing
CN112280828A (zh) * 2020-10-22 2021-01-29 上海交通大学医学院 一种降低单细胞扩增偏倚性的离体组织细胞核分离方法
CN112309500B (zh) * 2020-10-30 2024-08-30 广州序科码生物技术有限责任公司 一种基于单细胞测序数据唯一片段序列捕获方法
WO2022109207A2 (en) 2020-11-19 2022-05-27 The United States Of America As Represented By The Secretary, Department Of Health And Human Services Massively paralleled multi-patient assay for pathogenic infection diagnosis and host physiology surveillance using nucleic acid sequencing
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis
CN114686564B (zh) * 2020-12-31 2023-10-24 深圳华大生命科学研究院 一种适用于微量样本的dna样本制备方法
CN114836838A (zh) * 2021-02-01 2022-08-02 南方医科大学 一种中通量单细胞拷贝数文库构建的方法及其应用
AU2022227563A1 (en) 2021-02-23 2023-08-24 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
WO2022188054A1 (en) * 2021-03-10 2022-09-15 Nanjing University Methods and reagents for sample multiplexing for high throughput single-cell rna sequencing
AU2022245985A1 (en) 2021-03-22 2023-09-21 Illumina Cambridge Limited Methods for improving nucleic acid cluster clonality
EP4328362A4 (en) * 2021-04-22 2024-07-31 BGI Shenzhen CONSTRUCTION METHOD FOR RNA SEQUENCING LIBRARY, SEQUENCING METHOD AND KIT
WO2022271954A1 (en) 2021-06-24 2022-12-29 Illumina, Inc. Methods and compositions for combinatorial indexing of bead-based nucleic acids
CN114277093B (zh) * 2021-12-24 2024-02-27 中国农业科学院生物技术研究所 一种提取植物细胞核的方法
CN114277094B (zh) * 2021-12-24 2024-02-27 中国农业科学院生物技术研究所 提取植物细胞核的裂解液
IL315876A (en) 2022-04-07 2024-11-01 Illumina Inc Modification of cytidine deaminases and methods of use
WO2024073043A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of using cpg binding proteins in mapping modified cytosine nucleotides
EP4594481A1 (en) 2022-09-30 2025-08-06 Illumina, Inc. Helicase-cytidine deaminase complexes and methods of use
WO2024073047A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides
WO2024118903A1 (en) 2022-11-30 2024-06-06 Illumina, Inc. Chemoenzymatic correction of false positive uracil transformations
EP4646491A1 (en) 2023-01-06 2025-11-12 Illumina, Inc. Reducing uracils by polymerase
WO2024249466A1 (en) 2023-05-31 2024-12-05 Illumina, Inc. False positive reduction by translesion polymerase repair
WO2025072800A2 (en) 2023-09-28 2025-04-03 Illumina, Inc. Altered cytidine deaminases and methods of use
WO2025081064A2 (en) 2023-10-11 2025-04-17 Illumina, Inc. Thermophilic deaminase and methods for identifying modified cytosine
WO2025137222A1 (en) 2023-12-19 2025-06-26 Illumina, Inc. Methylation detection assay
WO2025208123A1 (en) * 2024-03-28 2025-10-02 The Regents Of The University Of California Methods and systems for profiling chromatin architecture
CN118272509A (zh) * 2024-03-28 2024-07-02 浙江大学 基于组合索引的单细胞dna-蛋白互作测序试剂盒和方法

Family Cites Families (87)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4882268A (en) 1985-12-24 1989-11-21 Massachusetts Institute Of Technology Method for determining tissue of origin and degree of malignancy of tumor cells
AU622426B2 (en) 1987-12-11 1992-04-09 Abbott Laboratories Assay using template-dependent nucleic acid probe reorganization
CA1341584C (en) 1988-04-06 2008-11-18 Bruce Wallace Method of amplifying and detecting nucleic acid sequences
WO1989009835A1 (en) 1988-04-08 1989-10-19 The Salk Institute For Biological Studies Ligase-based amplification method
EP0379559B1 (en) 1988-06-24 1996-10-23 Amgen Inc. Method and reagents for detecting nucleic acid sequences
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
JP2955759B2 (ja) 1988-07-20 1999-10-04 セゲブ・ダイアグノスティックス・インコーポレイテッド 核酸配列を増幅及び検出する方法
US5185243A (en) 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
EP0450060A1 (en) 1989-10-26 1991-10-09 Sri International Dna sequencing
US5573907A (en) 1990-01-26 1996-11-12 Abbott Laboratories Detecting and amplifying target nucleic acids using exonucleolytic activity
EP0439182B1 (en) 1990-01-26 1996-04-24 Abbott Laboratories Improved method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions
US5223414A (en) 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
EP0754240B1 (en) 1994-02-07 2003-08-20 Beckman Coulter, Inc. Ligase/polymerase-mediated genetic bit analysis of single nucleotide polymorphisms and its use in genetic analysis
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
AU687535B2 (en) 1994-03-16 1998-02-26 Gen-Probe Incorporated Isothermal strand displacement nucleic acid amplification
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
JP2002503954A (ja) 1997-04-01 2002-02-05 グラクソ、グループ、リミテッド 核酸増幅法
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
US6245974B1 (en) * 1997-08-06 2001-06-12 North Carolina State University Matrix attachment regions
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
CN101525660A (zh) 2000-07-07 2009-09-09 维西根生物技术公司 实时序列测定
EP1354064A2 (en) 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
EP3795577A1 (en) 2002-08-23 2021-03-24 Illumina Cambridge Limited Modified nucleotides
EP1539979B1 (en) 2002-09-20 2008-11-19 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
CA2527947A1 (en) 2003-06-06 2004-12-16 The Board Of Trustees Of The University Of Illinois Methods for assessing the invasive potential of a cell employing chromatin analysis
US20050053980A1 (en) 2003-06-20 2005-03-10 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
EP3175914A1 (en) 2004-01-07 2017-06-07 Illumina Cambridge Limited Improvements in or relating to molecular arrays
US7315019B2 (en) 2004-09-17 2008-01-01 Pacific Biosciences Of California, Inc. Arrays of optical confinements and uses thereof
EP1828412B2 (en) 2004-12-13 2019-01-09 Illumina Cambridge Limited Improved method of nucleotide detection
US8623628B2 (en) 2005-05-10 2014-01-07 Illumina, Inc. Polymerases
ES2434915T3 (es) * 2005-06-20 2013-12-18 Advanced Cell Diagnostics, Inc. Detección múltiplex de ácidos nucleicos
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
US20080009420A1 (en) 2006-03-17 2008-01-10 Schroth Gary P Isothermal methods for creating clonal single molecule arrays
EP3722409A1 (en) 2006-03-31 2020-10-14 Illumina, Inc. Systems and devices for sequence by synthesis analysis
WO2008051530A2 (en) 2006-10-23 2008-05-02 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
EP4134667B1 (en) 2006-12-14 2025-11-12 Life Technologies Corporation Apparatus for measuring analytes using fet arrays
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US8198028B2 (en) 2008-07-02 2012-06-12 Illumina Cambridge Limited Using populations of beads for the fabrication of arrays on surfaces
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
EP2635679B1 (en) 2010-11-05 2017-04-19 Illumina, Inc. Linking sequence reads using paired code tags
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
US8829171B2 (en) 2011-02-10 2014-09-09 Illumina, Inc. Linking sequence reads using paired code tags
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
EP2714938B1 (en) * 2011-05-27 2017-11-15 President and Fellows of Harvard College Methods of amplifying whole genome of a single cell
WO2012170560A2 (en) * 2011-06-06 2012-12-13 Cornell University Microfluidic device for extracting, isolating, and analyzing dna from cells
US8778848B2 (en) 2011-06-09 2014-07-15 Illumina, Inc. Patterned flow-cells useful for nucleic acid analysis
CA2859660C (en) 2011-09-23 2021-02-09 Illumina, Inc. Methods and compositions for nucleic acid sequencing
CA3003082C (en) 2011-10-28 2020-12-15 Illumina, Inc. Microarray fabrication system and method
EP3366348B1 (en) 2012-01-16 2023-08-23 Greatbatch Ltd. Emi filtered co-connected hermetic feedthrough, feedthrough capacitor and leadwire assembly for an active implantable medical device
JP6159391B2 (ja) 2012-04-03 2017-07-05 イラミーナ インコーポレーテッド 核酸シークエンシングに有用な統合化した読取りヘッド及び流体カートリッジ
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
JP2015523087A (ja) * 2012-07-24 2015-08-13 シーケンタ インコーポレイテッド 配列タグを用いる単一細胞分析
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
DK3553175T3 (da) 2013-03-13 2021-08-23 Illumina Inc Fremgangsmåde til fremstilling af et nukleinsyresekvenseringsbibliotek
WO2014145078A1 (en) * 2013-03-15 2014-09-18 Verinata Health, Inc. Generating cell-free dna libraries directly from blood
WO2014151554A1 (en) * 2013-03-15 2014-09-25 The Trustees Of The University Of Pennsylvania Phi29 method for library preparation
EP4617376A2 (en) * 2013-05-23 2025-09-17 The Board of Trustees of the Leland Stanford Junior University Transposition into native chromatin for personal epigenomics
CN105431554B (zh) 2013-07-01 2019-02-15 Illumina公司 无催化剂的表面官能化和聚合物接枝
US9677132B2 (en) 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
SG11201610910QA (en) 2014-06-30 2017-01-27 Illumina Inc Methods and compositions using one-sided transposition
KR102643955B1 (ko) * 2014-10-17 2024-03-07 일루미나 케임브리지 리미티드 근접 보존 전위
DK3212684T3 (da) 2014-10-31 2020-03-02 Illumina Cambridge Ltd Polymerer og DNA-copolymer-belægninger
GB2532790B (en) 2014-11-28 2017-10-04 Porvair Filtration Group Ltd Modified pipette tips for chromatin immunoprecipitation assay
KR20240091073A (ko) 2015-02-10 2024-06-21 일루미나, 인코포레이티드 세포 성분을 분석하기 위한 방법 및 조성물
EP3868879A1 (en) * 2016-03-10 2021-08-25 The Board of Trustees of the Leland Stanford Junior University Transposase-mediated imaging of the accessible genome

Also Published As

Publication number Publication date
CA3026773A1 (en) 2018-01-25
KR102475710B1 (ko) 2022-12-08
CN110268059B (zh) 2024-01-12
WO2018018008A1 (en) 2018-01-25
EP3488002B1 (en) 2021-03-31
IL263920B2 (en) 2023-02-01
AU2017299803A1 (en) 2019-01-24
ES2873723T3 (es) 2021-11-03
AU2017299803B2 (en) 2023-06-29
US11535883B2 (en) 2022-12-27
SG11201811504PA (en) 2019-01-30
KR20230003255A (ko) 2023-01-05
JP2019520828A (ja) 2019-07-25
KR102709499B1 (ko) 2024-09-24
AU2025237993A1 (en) 2025-10-16
JP7155021B2 (ja) 2022-10-18
EP3488002A1 (en) 2019-05-29
IL263920A (en) 2019-01-31
US20180023119A1 (en) 2018-01-25
AU2023222858B2 (en) 2025-06-26
CN117822128A (zh) 2024-04-05
JP7564158B2 (ja) 2024-10-08
JP2022169578A (ja) 2022-11-09
KR20190034164A (ko) 2019-04-01
IL263920B (en) 2022-10-01
CN110268059A (zh) 2019-09-20
AU2023222858A1 (en) 2023-09-28
US20230323426A1 (en) 2023-10-12
CA3026773C (en) 2022-10-18
EP3904514A1 (en) 2021-11-03
NZ749719A (en) 2021-06-25

Similar Documents

Publication Publication Date Title
DK3488002T3 (da) Enkeltcelle-helgenombiblioteker og kombinatoriske indekseringsfremgangsmåder til fremstilling deraf
DK3635136T3 (da) Enkeltcelle-helgenombiblioteker til methyleringssekvensering
DK3548652T3 (da) Fremgangsmåder til proteinanalyse
DK3265947T3 (da) Fremgangsmåde og system til kryptering
DK3325669T3 (da) Sammensætninger og fremgangsmåder til RNA-analyse
DK3356413T3 (da) Anti-tigit-antigenbindingsproteiner og fremgangsmåder til anvendelse deraf
DK3215601T3 (da) Fremgangsmåder til transduktion og celleforarbejdning
DK3260813T3 (da) System og fremgangsmåde til afstandsbedømmelse
DK3267884T3 (da) Systemer, apparater og fremgangsmåder til måling af føtal aktivitet
DK3475625T3 (da) Styresystem til renrum og fremgangsmåde
DK3280441T3 (da) Anti-sortilin-antistoffer og fremgangsmåder til anvendelse deraf
DK3553180T3 (da) Fremgangsmåde til konstruktion af enkeltcellesekventeringsbibliotek og anvendelse deraf
DK3149025T3 (da) Cellepenetrerende peptider og fremgangsmåder til frembringelse og anvendelse deraf
DK3058092T3 (da) Fremgangsmåder og præparater til fremstilling af nukleinsyrebiblioteker
IL257453A (en) Modular polypeptide libraries and methods of making and using same
DK3532838T3 (da) Fremgangsmåder og systemer til kromatografidataanalyse
DK3213065T3 (da) System og fremgangsmåder til todimensionel rplc-sfc-kromatografi
DK3129912T3 (da) Fremgangsmåde og system til sikring af data
DK3368663T3 (da) Virusfri cellelinjer og fremgangsmåder til opnåelse deraf
DK3177608T3 (da) Proteinkinase-C-hæmmere og fremgangsmåder til anvendelse deraf
DK3319612T3 (da) Oxysteroler og fremgansmåder til anvendelse derfor
DK3551787T3 (da) Elektropoleringsfremgangsmåde og anlæg hertil
DK3998285T3 (da) Bindingsmolekyler til cd38 og pd-l1
EP3488225A4 (en) GAS MEASUREMENT SYSTEM
DK3155543T3 (da) Databehandlingsenhed og fremgangsmåde til evaluering af massespektrometridata