DK3553180T3 - Fremgangsmåde til konstruktion af enkeltcellesekventeringsbibliotek og anvendelse deraf - Google Patents
Fremgangsmåde til konstruktion af enkeltcellesekventeringsbibliotek og anvendelse deraf Download PDFInfo
- Publication number
- DK3553180T3 DK3553180T3 DK16923153.7T DK16923153T DK3553180T3 DK 3553180 T3 DK3553180 T3 DK 3553180T3 DK 16923153 T DK16923153 T DK 16923153T DK 3553180 T3 DK3553180 T3 DK 3553180T3
- Authority
- DK
- Denmark
- Prior art keywords
- procedure
- single cell
- library construction
- sequence library
- cell sequence
- Prior art date
Links
- 238000010276 construction Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2016/108940 WO2018103025A1 (zh) | 2016-12-07 | 2016-12-07 | 单细胞测序文库的构建方法及其应用 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3553180T3 true DK3553180T3 (da) | 2022-07-25 |
Family
ID=62490570
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK16923153.7T DK3553180T3 (da) | 2016-12-07 | 2016-12-07 | Fremgangsmåde til konstruktion af enkeltcellesekventeringsbibliotek og anvendelse deraf |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US11434483B2 (da) |
| EP (1) | EP3553180B1 (da) |
| CN (1) | CN109996892B (da) |
| DK (1) | DK3553180T3 (da) |
| ES (1) | ES2922281T3 (da) |
| WO (1) | WO2018103025A1 (da) |
Families Citing this family (61)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CA2881685C (en) | 2012-08-14 | 2023-12-05 | 10X Genomics, Inc. | Microcapsule compositions and methods |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10584381B2 (en) | 2012-08-14 | 2020-03-10 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| EP2931919B1 (en) | 2012-12-14 | 2019-02-20 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| JP2016511243A (ja) | 2013-02-08 | 2016-04-14 | テンエックス・ジェノミクス・インコーポレイテッド | ポリヌクレオチドバーコード生成 |
| CA2943624A1 (en) | 2014-04-10 | 2015-10-15 | 10X Genomics, Inc. | Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same |
| WO2015200893A2 (en) | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
| EP3161162A4 (en) | 2014-06-26 | 2018-01-10 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| US12312640B2 (en) | 2014-06-26 | 2025-05-27 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| EP3244992B1 (en) | 2015-01-12 | 2023-03-08 | 10X Genomics, Inc. | Processes for barcoding nucleic acids |
| EP3262407B1 (en) | 2015-02-24 | 2023-08-30 | 10X Genomics, Inc. | Partition processing methods and systems |
| US12264411B2 (en) | 2017-01-30 | 2025-04-01 | 10X Genomics, Inc. | Methods and systems for analysis |
| EP4310183B1 (en) | 2017-01-30 | 2025-07-09 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
| EP4230746A3 (en) | 2017-05-26 | 2023-11-01 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| US10844372B2 (en) | 2017-05-26 | 2020-11-24 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| WO2019084043A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS |
| WO2019099751A1 (en) | 2017-11-15 | 2019-05-23 | 10X Genomics, Inc. | Functionalized gel beads |
| US20200354767A1 (en) * | 2017-11-16 | 2020-11-12 | The Regents Of The University Of Colorado, A Body Corporate | Methods to measure functional heterogeneity among single cells |
| US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
| CN112005115A (zh) | 2018-02-12 | 2020-11-27 | 10X基因组学有限公司 | 表征来自单个细胞或细胞群体的多种分析物的方法 |
| CN112262218B (zh) | 2018-04-06 | 2024-11-08 | 10X基因组学有限公司 | 用于单细胞处理中的质量控制的系统和方法 |
| US11932899B2 (en) | 2018-06-07 | 2024-03-19 | 10X Genomics, Inc. | Methods and systems for characterizing nucleic acid molecules |
| WO2020009665A1 (en) * | 2018-07-06 | 2020-01-09 | Agency For Science, Technology And Research | Method for single-cell transcriptome and accessible regions sequencing |
| EP3830289A1 (en) | 2018-08-03 | 2021-06-09 | 10X Genomics, Inc. | Methods and systems to minimize barcode exchange |
| US12065688B2 (en) | 2018-08-20 | 2024-08-20 | 10X Genomics, Inc. | Compositions and methods for cellular processing |
| CN109023537B (zh) * | 2018-09-04 | 2021-10-08 | 上海交通大学 | 一种微量dna样品高通量测序文库的构建技术 |
| AU2020206999B2 (en) | 2019-01-07 | 2025-10-02 | Agilent Technologies, Inc. | Compositions and methods for genomic dna and gene expression analysis in single cells |
| US11845983B1 (en) | 2019-01-09 | 2023-12-19 | 10X Genomics, Inc. | Methods and systems for multiplexing of droplet based assays |
| US11467153B2 (en) | 2019-02-12 | 2022-10-11 | 10X Genomics, Inc. | Methods for processing nucleic acid molecules |
| EP3924505A1 (en) | 2019-02-12 | 2021-12-22 | 10X Genomics, Inc. | Methods for processing nucleic acid molecules |
| WO2020167866A1 (en) | 2019-02-12 | 2020-08-20 | 10X Genomics, Inc. | Systems and methods for transposon loading |
| EP3950956A4 (en) * | 2019-05-21 | 2022-05-04 | MGI Tech Co., Ltd. | Method and system for constructing sequencing library on the basis of methylated dna target region, and use thereof |
| CN110952147B (zh) * | 2019-12-18 | 2023-05-05 | 南方科技大学 | 单细胞基因组测序用的dna文库的构建方法 |
| EP4095256A4 (en) * | 2020-01-23 | 2023-03-15 | DGI Tech (Qing Dao) Co., Limited | Droplet microfluidics-based single cell sequencing and applications |
| WO2021226917A1 (en) * | 2020-05-14 | 2021-11-18 | Singleron (Nanjing) Biotechnologies, Ltd. | Novel method of one-step whole transcriptome amplification |
| CN111575348B (zh) * | 2020-05-19 | 2024-01-09 | 广州微远医疗器械有限公司 | 宏基因组文库及建库方法和应用 |
| CN112011596A (zh) * | 2020-09-11 | 2020-12-01 | 深圳华大因源医药科技有限公司 | 一种同时检测多种微生物基因组的方法、溶液和试剂盒 |
| CN114045332A (zh) * | 2020-09-29 | 2022-02-15 | 生物岛实验室 | 一种单细胞表观转录组学测序方法 |
| CN112280828A (zh) * | 2020-10-22 | 2021-01-29 | 上海交通大学医学院 | 一种降低单细胞扩增偏倚性的离体组织细胞核分离方法 |
| CN112853503A (zh) * | 2021-03-24 | 2021-05-28 | 北京百迈客生物科技有限公司 | 用于纳米孔测序的单细胞或微量样本全长转录组文库构建方法 |
| CN112941636A (zh) * | 2021-04-02 | 2021-06-11 | 武汉博越致和生物科技有限公司 | 一种适用于简化转录组测序的文库构建方法及配套试剂盒 |
| CN113462748A (zh) * | 2021-05-11 | 2021-10-01 | 温氏食品集团股份有限公司 | Dna测序文库的制备方法及试剂盒 |
| CN113249439A (zh) * | 2021-05-11 | 2021-08-13 | 杭州圣庭医疗科技有限公司 | 一种简化dna甲基化文库及转录组共测序文库的构建方法 |
| CN113337590B (zh) * | 2021-06-03 | 2024-07-09 | 深圳华大基因股份有限公司 | 一种二代测序方法和文库构建方法 |
| CN116694730B (zh) * | 2022-02-28 | 2025-02-14 | 南方科技大学 | 一种单细胞开放染色质和转录组共测序文库的构建方法 |
| CN114645074A (zh) * | 2022-03-07 | 2022-06-21 | 华南农业大学 | 一种单花蕾微量rna建库方法 |
| CN115851876A (zh) * | 2022-07-21 | 2023-03-28 | 浙江大学绍兴研究院 | 一种同时获取全基因组转录和蛋白-dna结合信息的测序方法 |
| CN115612720B (zh) * | 2022-09-28 | 2025-03-14 | 上海欧易生物医学科技有限公司 | 一种适用于猕猴桃根组织单细胞测序的植物细胞核制备以及悬液优化方法 |
| CN115478098A (zh) * | 2022-10-10 | 2022-12-16 | 中国科学技术大学 | 一种单细胞转录组及染色质可及性双组学测序文库构建方法及测序方法 |
| WO2024077439A1 (zh) * | 2022-10-10 | 2024-04-18 | 中国科学技术大学 | 一种单细胞转录组及染色质可及性双组学测序文库构建方法及测序方法 |
| CN120092087A (zh) * | 2022-10-21 | 2025-06-03 | 深圳华大生命科学研究院 | 多组学的文库构建方法、检测方法及相关试剂盒 |
| CN115948621A (zh) * | 2023-01-18 | 2023-04-11 | 珠海舒桐医疗科技有限公司 | 一种基于月经血dna的hpv筛查方法 |
| CN116121175A (zh) * | 2023-01-19 | 2023-05-16 | 同济大学 | 一种同一单细胞中细胞核与细胞质的分离方法与应用 |
| WO2024250155A1 (zh) * | 2023-06-05 | 2024-12-12 | 清华大学 | 一种构建单细胞测序文库的方法 |
| CN117448424A (zh) * | 2023-11-29 | 2024-01-26 | 上海欧易生物医学科技有限公司 | 一种适用于单细胞核全转录组测序的建库方法、试剂盒及应用 |
| CN120608127B (zh) * | 2025-08-12 | 2025-11-28 | 北京寻因生物科技有限公司 | 一种提高细胞中染色质dna可及性的方法及其应用 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS53122047A (en) | 1977-03-30 | 1978-10-25 | Sanden Corp | Electromagnetic clutch |
| JPS5924538U (ja) | 1982-08-09 | 1984-02-15 | サンデン株式会社 | 電磁クラツチ |
| JP4174896B2 (ja) | 1998-09-22 | 2008-11-05 | 株式会社デンソー | 電磁クラッチ |
| KR20040034191A (ko) | 2002-10-21 | 2004-04-28 | 한라공조주식회사 | 전자클러치용 디스크 및 허브 조립체 |
| US7044284B2 (en) | 2003-06-10 | 2006-05-16 | Halla Climate Control Corporation | Disc and hub assembly for electromagnetic clutch in a compressor |
| EP4617376A2 (en) * | 2013-05-23 | 2025-09-17 | The Board of Trustees of the Leland Stanford Junior University | Transposition into native chromatin for personal epigenomics |
| CN104561244B (zh) * | 2013-10-15 | 2017-03-22 | 天津大学 | 基于二代测序技术的微生物单细胞转录组分析方法 |
| CN106507677B (zh) * | 2014-04-15 | 2021-03-12 | 伊鲁米那股份有限公司 | 用于改进插入序列偏倚和增加dna输入耐受性的修饰的转座酶 |
| CN105463089A (zh) * | 2015-12-21 | 2016-04-06 | 同济大学 | 应用于斑马鱼胚胎的易接近转座酶核染色质高通量测序实验的方法 |
-
2016
- 2016-12-07 DK DK16923153.7T patent/DK3553180T3/da active
- 2016-12-07 EP EP16923153.7A patent/EP3553180B1/en active Active
- 2016-12-07 US US16/466,741 patent/US11434483B2/en active Active
- 2016-12-07 CN CN201680091050.2A patent/CN109996892B/zh active Active
- 2016-12-07 ES ES16923153T patent/ES2922281T3/es active Active
- 2016-12-07 WO PCT/CN2016/108940 patent/WO2018103025A1/zh not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| US20190338279A1 (en) | 2019-11-07 |
| CN109996892B (zh) | 2023-08-29 |
| EP3553180A4 (en) | 2020-08-12 |
| EP3553180B1 (en) | 2022-05-04 |
| ES2922281T3 (es) | 2022-09-12 |
| EP3553180A1 (en) | 2019-10-16 |
| US11434483B2 (en) | 2022-09-06 |
| CN109996892A (zh) | 2019-07-09 |
| WO2018103025A1 (zh) | 2018-06-14 |
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