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MX2019006365A - Amplificación por exclusión cinética de bibliotecas de ácido nucleico. - Google Patents

Amplificación por exclusión cinética de bibliotecas de ácido nucleico.

Info

Publication number
MX2019006365A
MX2019006365A MX2019006365A MX2019006365A MX2019006365A MX 2019006365 A MX2019006365 A MX 2019006365A MX 2019006365 A MX2019006365 A MX 2019006365A MX 2019006365 A MX2019006365 A MX 2019006365A MX 2019006365 A MX2019006365 A MX 2019006365A
Authority
MX
Mexico
Prior art keywords
solution
nucleic acids
target nucleic
amplification
amplification sites
Prior art date
Application number
MX2019006365A
Other languages
English (en)
Other versions
MX392656B (es
Inventor
Hunter Shaun
McInerney Peter
Boutell Jonathan
Bevis-Mott Claire
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of MX2019006365A publication Critical patent/MX2019006365A/es
Publication of MX392656B publication Critical patent/MX392656B/es

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
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  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Un método ejemplar incluye hacer reaccionar una primera solución y una segunda solución, diferente en una celda de flujo al hacer fluir la primera solución sobre sitios de amplificación en la celda de flujo y hacer fluir subsecuentemente la segunda solución sobre los sitios de amplificación. La primera solución incluye ácidos nucleicos diana y una primera mezcla de reactivos que comprende nucleósido-trifosfatos y enzimas de replicación. Los ácidos nucleicos diana en la primera solución se transportan a y se enlazan a los sitios de amplificación a una velocidad de transporte. La primera mezcla de reactivos amplifica los ácidos nucleicos diana que se enlazan a los sitios de amplificación para producir poblaciones clonales de amplicones que se originan de ácidos nucleicos diana correspondientes. Los amplicones se producen a una velocidad de amplificación que excede la velocidad de transporte. La segunda solución incluye una segunda mezcla de reactivos y carece de los ácidos nucleicos diana. La segunda solución es para incrementar un número de los amplicones en los sitios de amplificación.
MX2019006365A 2017-01-05 2017-12-15 Amplificación por exclusión cinética de bibliotecas de ácido nucleico. MX392656B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762442680P 2017-01-05 2017-01-05
GBGB1704754.9A GB201704754D0 (en) 2017-01-05 2017-03-24 Kinetic exclusion amplification of nucleic acid libraries
PCT/US2017/066751 WO2018128777A1 (en) 2017-01-05 2017-12-15 Kinetic exclusion amplification of nucleic acid libraries

Publications (2)

Publication Number Publication Date
MX2019006365A true MX2019006365A (es) 2019-10-15
MX392656B MX392656B (es) 2025-03-24

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MX2019006365A MX392656B (es) 2017-01-05 2017-12-15 Amplificación por exclusión cinética de bibliotecas de ácido nucleico.
MX2022006265A MX2022006265A (es) 2017-01-05 2019-05-30 Amplificacion por exclusion cinetica de bibliotecas de acido nucleico.

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US (2) US10808277B2 (es)
EP (1) EP3565901B1 (es)
JP (2) JP7022754B2 (es)
KR (1) KR102292759B1 (es)
CN (1) CN110268070A (es)
AU (2) AU2017390190A1 (es)
BR (1) BR112019012497A2 (es)
CA (1) CA3046028C (es)
ES (1) ES3030504T3 (es)
GB (1) GB201704754D0 (es)
IL (1) IL267742B2 (es)
MX (2) MX392656B (es)
MY (2) MY193199A (es)
RU (1) RU2759690C2 (es)
TW (1) TWI776830B (es)
WO (1) WO2018128777A1 (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11426318B2 (en) 2020-05-20 2022-08-30 Augustine Biomedical + Design, LLC Medical module including automated dose-response record system
US11432982B2 (en) 2018-03-26 2022-09-06 Augustine Biomedical + Design, LLC Relocation module and methods for surgical equipment
US11291602B2 (en) 2018-03-26 2022-04-05 Augustine Biomedical + Design, LLC Relocation module and methods for surgical equipment
US11219570B2 (en) 2018-03-26 2022-01-11 Augustine Biomedical + Design, LLC Relocation module and methods for surgical equipment
US10869800B2 (en) 2018-03-26 2020-12-22 Augustine Biomedical + Design, LLC Relocation module and methods for surgical equipment
US10507153B2 (en) 2018-03-26 2019-12-17 Augustine Biomedical + Design, LLC Relocation modules and methods for surgical field
US11160710B1 (en) 2020-05-20 2021-11-02 Augustine Biomedical + Design, LLC Relocation module and methods for surgical equipment
EP3976751A4 (en) * 2019-05-31 2023-01-11 Illumina, Inc. FLOW CELL WITH ONE OR MORE BARRIER PROPERTIES
TWI860366B (zh) 2019-06-19 2024-11-01 美商伊路米納有限公司 儀器中的試劑交換
CN113646440B (zh) * 2019-12-02 2025-02-07 因美纳剑桥有限公司 基因组dna的扩增邻近性保留文库片段的基于时间的簇成像
WO2021168097A1 (en) * 2020-02-19 2021-08-26 Genapsys, Inc. Methods and systems for nucleic acid processing
CA3182608A1 (en) * 2020-06-16 2021-12-23 Carl Martin Systems, apparatus and kits for enzymatic polynucleotide synthesis
MX2022014724A (es) 2020-09-14 2023-03-06 Illumina Inc Composiciones y metodos para amplificar polinucleótidos.
CN114517145A (zh) * 2020-11-20 2022-05-20 京东方科技集团股份有限公司 生物分子捕获机构和微流控芯片
WO2023245203A1 (en) * 2022-06-18 2023-12-21 Reticula, Inc. Methods for single cell sequencing and error rate reduction
CN117542391A (zh) * 2022-08-01 2024-02-09 上海交通大学 一种数据存储介质及其应用
US20240110221A1 (en) * 2022-09-30 2024-04-04 Illumina, Inc. Methods of modulating clustering kinetics

Family Cites Families (85)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8810400D0 (en) 1988-05-03 1988-06-08 Southern E Analysing polynucleotide sequences
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
EP0450060A1 (en) 1989-10-26 1991-10-09 Sri International Dna sequencing
US5223414A (en) 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5556773A (en) 1993-08-06 1996-09-17 Yourno; Joseph Method and apparatus for nested polymerase chain reaction (PCR) with single closed reaction tubes
AU687535B2 (en) 1994-03-16 1998-02-26 Gen-Probe Incorporated Isothermal strand displacement nucleic acid amplification
US5552278A (en) 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US6023540A (en) 1997-03-14 2000-02-08 Trustees Of Tufts College Fiber optic sensor with encoded microspheres
US6327410B1 (en) 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
ATE269908T1 (de) 1997-04-01 2004-07-15 Manteia S A Methode zur sequenzierung von nukleinsäuren
US6391622B1 (en) * 1997-04-04 2002-05-21 Caliper Technologies Corp. Closed-loop biochemical analyzers
US6511803B1 (en) 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
ATE413467T1 (de) 1999-04-20 2008-11-15 Illumina Inc Detektion von nukleinsäurereaktionen auf kügelchen-arrays
US20050181440A1 (en) 1999-04-20 2005-08-18 Illumina, Inc. Nucleic acid sequencing using microsphere arrays
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US6913884B2 (en) 2001-08-16 2005-07-05 Illumina, Inc. Compositions and methods for repetitive use of genomic DNA
WO2001057269A2 (en) 2000-02-07 2001-08-09 Illumina, Inc. Nucleic acid detection methods using universal priming
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
CN101525660A (zh) 2000-07-07 2009-09-09 维西根生物技术公司 实时序列测定
EP1354064A2 (en) 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US20040002090A1 (en) 2002-03-05 2004-01-01 Pascal Mayer Methods for detecting genome-wide sequence variations associated with a phenotype
EP3795577A1 (en) 2002-08-23 2021-03-24 Illumina Cambridge Limited Modified nucleotides
US7595883B1 (en) 2002-09-16 2009-09-29 The Board Of Trustees Of The Leland Stanford Junior University Biological analysis arrangement and approach therefor
EP1539979B1 (en) 2002-09-20 2008-11-19 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
ATE546525T1 (de) 2003-01-29 2012-03-15 454 Life Sciences Corp Nukleinsäureamplifikation auf basis von kügelchenemulsion
US20050053980A1 (en) 2003-06-20 2005-03-10 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
CA2531105C (en) 2003-07-05 2015-03-17 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
EP3175914A1 (en) 2004-01-07 2017-06-07 Illumina Cambridge Limited Improvements in or relating to molecular arrays
US7315019B2 (en) 2004-09-17 2008-01-01 Pacific Biosciences Of California, Inc. Arrays of optical confinements and uses thereof
US7709197B2 (en) 2005-06-15 2010-05-04 Callida Genomics, Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
US20080009420A1 (en) 2006-03-17 2008-01-10 Schroth Gary P Isothermal methods for creating clonal single molecule arrays
EP3722409A1 (en) 2006-03-31 2020-10-14 Illumina, Inc. Systems and devices for sequence by synthesis analysis
WO2008002502A2 (en) 2006-06-23 2008-01-03 Illumina, Inc. Devices and systems for creation of dna cluster arrays
WO2008051530A2 (en) 2006-10-23 2008-05-02 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
EP4134667B1 (en) 2006-12-14 2025-11-12 Life Technologies Corporation Apparatus for measuring analytes using fet arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
WO2009012112A1 (en) 2007-07-13 2009-01-22 The Board Of Trustees Of The Leland Stanford Junior University Method and apparatus using electric field for improved biological assays
US20090226975A1 (en) 2008-03-10 2009-09-10 Illumina, Inc. Constant cluster seeding
HRP20160923T1 (hr) * 2008-04-03 2016-10-21 Cb Biotechnologies, Inc. Oslobađanje amlikona višestrukom (multiplex) lančanom reakcijom polimeraze za umnožavanje više ciljeva
US20100035252A1 (en) 2008-08-08 2010-02-11 Ion Torrent Systems Incorporated Methods for sequencing individual nucleic acids under tension
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US8951778B2 (en) 2008-12-19 2015-02-10 Xyleco, Inc. Processing biomass
US8716467B2 (en) * 2010-03-03 2014-05-06 Gen9, Inc. Methods and devices for nucleic acid synthesis
WO2011156846A1 (en) * 2010-06-17 2011-12-22 Geneasys Pty Ltd Microfluidic device for simultaneous detection of multiple conditions in a patient
WO2011159942A1 (en) 2010-06-18 2011-12-22 Illumina, Inc. Conformational probes and methods for sequencing nucleic acids
EP3572528A1 (en) * 2010-09-24 2019-11-27 The Board of Trustees of the Leland Stanford Junior University Direct capture, amplification and sequencing of target dna using immobilized primers
EP2633069B1 (en) 2010-10-26 2015-07-01 Illumina, Inc. Sequencing methods
EP3928867B1 (en) 2010-10-27 2024-07-17 Illumina, Inc. Microdevices and biosensor cartridges for biological or chemical analysis and systems and methods for the same
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
WO2012106072A2 (en) * 2011-02-03 2012-08-09 Illumina, Inc. Methods for minimizing sequence specific bias
US8778848B2 (en) 2011-06-09 2014-07-15 Illumina, Inc. Patterned flow-cells useful for nucleic acid analysis
US10378051B2 (en) 2011-09-29 2019-08-13 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
CA3003082C (en) 2011-10-28 2020-12-15 Illumina, Inc. Microarray fabrication system and method
US9279154B2 (en) 2011-12-21 2016-03-08 Illumina, Inc. Apparatus and methods for kinetic analysis and determination of nucleic acid sequences
US9803239B2 (en) * 2012-03-29 2017-10-31 Complete Genomics, Inc. Flow cells for high density array chips
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
CA3009218C (en) 2013-08-08 2020-10-27 Illumina, Inc. Fluidic system for reagent delivery to a flow cell
US9944924B2 (en) 2014-01-16 2018-04-17 Illumina, Inc. Polynucleotide modification on solid support
EP2921556A1 (en) 2014-03-21 2015-09-23 Lexogen GmbH Copy number preserving RNA analysis method
US10570447B2 (en) 2014-05-16 2020-02-25 Illumina, Inc. Nucleic acid synthesis techniques
CA3172086A1 (en) 2014-06-13 2015-12-17 Illumina Cambridge Limited Methods and compositions for preparing sequencing libraries
WO2016054088A1 (en) 2014-09-29 2016-04-07 Illumina Cambridge Limited Recombinase mutants
SG11201703693UA (en) * 2014-11-11 2017-06-29 Illumina Cambridge Ltd Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid
CN114438172B (zh) 2014-12-15 2025-02-21 亿明达股份有限公司 用于基底上的单分子放置的组合物和方法
JP6609641B2 (ja) 2015-05-29 2019-11-20 イルミナ ケンブリッジ リミテッド クラスターにおける表面プライマーの高度な利用

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