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WO2017216472A3 - Variants d'une adn polymérase de la famille polx - Google Patents

Variants d'une adn polymérase de la famille polx Download PDF

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Publication number
WO2017216472A3
WO2017216472A3 PCT/FR2017/051519 FR2017051519W WO2017216472A3 WO 2017216472 A3 WO2017216472 A3 WO 2017216472A3 FR 2017051519 W FR2017051519 W FR 2017051519W WO 2017216472 A3 WO2017216472 A3 WO 2017216472A3
Authority
WO
WIPO (PCT)
Prior art keywords
variants
dna polymerase
polx family
nucleic acid
polx
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FR2017/051519
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English (en)
Other versions
WO2017216472A2 (fr
Inventor
Thomas YBERT
Marc Delarue
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
DNA Script SAS
Institut Pasteur
Original Assignee
Centre National de la Recherche Scientifique CNRS
DNA Script SAS
Institut Pasteur
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US16/309,414 priority Critical patent/US20200002690A1/en
Priority to CA3024184A priority patent/CA3024184A1/fr
Priority to KR1020187034921A priority patent/KR102522263B1/ko
Priority to AU2017286477A priority patent/AU2017286477C1/en
Priority to CN201780037427.0A priority patent/CN109477080A/zh
Priority to IL263503A priority patent/IL263503B2/en
Priority to JP2018564828A priority patent/JP7118510B2/ja
Priority to EP17746156.3A priority patent/EP3469075A2/fr
Application filed by Centre National de la Recherche Scientifique CNRS, DNA Script SAS, Institut Pasteur filed Critical Centre National de la Recherche Scientifique CNRS
Priority to SG11201809961TA priority patent/SG11201809961TA/en
Publication of WO2017216472A2 publication Critical patent/WO2017216472A2/fr
Publication of WO2017216472A3 publication Critical patent/WO2017216472A3/fr
Anticipated expiration legal-status Critical
Priority to JP2022105854A priority patent/JP2022137127A/ja
Priority to US17/815,490 priority patent/US20230167421A1/en
Priority to US18/516,616 priority patent/US20240124855A1/en
Priority to AU2023282219A priority patent/AU2023282219A1/en
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1264—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal nucleotidyl transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07031—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal deoxynucleotidyl transferase

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des variants d'une ADN polymérase de la famille polX capable de synthétiser une molécule d'acide nucléiquesans brin matrice, ou d'un fragment fonctionnel d'une telle polymérase, comprenant au moins une mutation d'un résidu à au moins une position particulière, et des utilisations de ces variants, notamment pour la synthèse de molécules d'acide nucléiques comprenant des nucléotides modifiés en 3'-OH.
PCT/FR2017/051519 2016-06-14 2017-06-13 Variants d'une adn polymérase de la famille polx Ceased WO2017216472A2 (fr)

Priority Applications (13)

Application Number Priority Date Filing Date Title
EP17746156.3A EP3469075A2 (fr) 2016-06-14 2017-06-13 Variants d'une adn polymérase de la famille polx
CA3024184A CA3024184A1 (fr) 2016-06-14 2017-06-13 Variants d'une adn polymerase de la famille polx
KR1020187034921A KR102522263B1 (ko) 2016-06-14 2017-06-13 polX 패밀리의 DNA 폴리머라제의 변형체
AU2017286477A AU2017286477C1 (en) 2016-06-14 2017-06-13 Variants of a DNA polymerase of the polX family
CN201780037427.0A CN109477080A (zh) 2016-06-14 2017-06-13 polX家族的DNA聚合酶的变体
IL263503A IL263503B2 (en) 2016-06-14 2017-06-13 DNA polymerase variants from the polX family
JP2018564828A JP7118510B2 (ja) 2016-06-14 2017-06-13 ｐｏｌＸファミリーＤＮＡポリメラーゼのバリアント
US16/309,414 US20200002690A1 (en) 2016-06-14 2017-06-13 Variants of a DNA Polymerase of the Polx Family
SG11201809961TA SG11201809961TA (en) 2016-06-14 2017-06-13 Variants of a dna polymerase of the polx family
JP2022105854A JP2022137127A (ja) 2016-06-14 2022-06-30 ｐｏｌＸファミリーＤＮＡポリメラーゼのバリアント
US17/815,490 US20230167421A1 (en) 2016-06-14 2022-07-27 Variants of a DNA Polymerase of the Polx Family
US18/516,616 US20240124855A1 (en) 2016-06-14 2023-11-21 Variants of a dna polymerase of the polx family
AU2023282219A AU2023282219A1 (en) 2016-06-14 2023-12-13 Variants of a DNA polymerase of the polX family

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1655475A FR3052462A1 (fr) 2016-06-14 2016-06-14 Variants d'une adn polymerase de la famille polx
FR1655475 2016-06-14

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US16/309,414 A-371-Of-International US20200002690A1 (en) 2016-06-14 2017-06-13 Variants of a DNA Polymerase of the Polx Family
US17/815,490 Continuation US20230167421A1 (en) 2016-06-14 2022-07-27 Variants of a DNA Polymerase of the Polx Family

Publications (2)

Publication Number Publication Date
WO2017216472A2 WO2017216472A2 (fr) 2017-12-21
WO2017216472A3 true WO2017216472A3 (fr) 2018-03-15

Family

ID=57348782

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2017/051519 Ceased WO2017216472A2 (fr) 2016-06-14 2017-06-13 Variants d'une adn polymérase de la famille polx

Country Status (11)

Country Link
US (3) US20200002690A1 (fr)
EP (1) EP3469075A2 (fr)
JP (2) JP7118510B2 (fr)
KR (1) KR102522263B1 (fr)
CN (1) CN109477080A (fr)
AU (2) AU2017286477C1 (fr)
CA (1) CA3024184A1 (fr)
FR (2) FR3052462A1 (fr)
IL (1) IL263503B2 (fr)
SG (2) SG10202009688QA (fr)
WO (1) WO2017216472A2 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10435676B2 (en) 2018-01-08 2019-10-08 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
US11359221B2 (en) 2019-02-12 2022-06-14 Dna Script Sas Efficient product cleavage in template-free enzymatic synthesis of polynucleotides
US12286652B2 (en) 2018-01-08 2025-04-29 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof

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US10774316B2 (en) * 2014-10-20 2020-09-15 Molecular Assemblies, Inc. Modified template-independent enzymes for polydeoxynucleotide synthesis
WO2018215803A1 (fr) 2017-05-26 2018-11-29 Nuclera Nucleics Ltd Utilisation d'enzyme transférase terminale dans la synthèse d'acides nucléiques
EP3665274B1 (fr) 2017-08-07 2025-10-15 DNA Script Variantes de la famille de polymérase d'adn et leurs utilisations
US12421628B2 (en) 2018-07-23 2025-09-23 Dna Script Massively parallel enzymatic synthesis of nucleic acid strands
WO2020099451A1 (fr) 2018-11-14 2020-05-22 Dna Script Variants de désoxynucléotidyltransférase et utilisations correspondantes
KR20210104779A (ko) 2018-12-13 2021-08-25 디엔에이 스크립트 세포 및 생체분자 상의 직접 올리고뉴클레오타이드 합성
CN113272442B (zh) 2019-01-03 2024-03-15 Dna斯克瑞普特公司 寡核苷酸的集合的一锅合成
GB201901501D0 (en) 2019-02-04 2019-03-27 Nuclera Nucleics Ltd Modified terminal deoxynucleotidyl transferase (TdT) enzymes
ES2982551T3 (es) * 2019-05-28 2024-10-16 Dna Script Variantes de desoxinucleotidil transferasa terminal y usos de las mismas
EP3744854A1 (fr) * 2019-05-28 2020-12-02 DNA Script Variants de désoxynucléotidyle transférase terminale et leurs utilisations
EP4004222A1 (fr) 2019-07-30 2022-06-01 DNA Script Synthèse enzymatique sans matrice de polynucléotides à l'aide de poly(a) et poly(u) polymérases
US20220403435A1 (en) 2019-08-01 2022-12-22 Dna Script Sas Increasing Long-Sequence Yields In Template-Free Enzymatic Synthesis of Polynucleotides
CN110331136B (zh) * 2019-09-05 2019-12-24 中国科学院天津工业生物技术研究所 一种末端脱氧核糖核苷转移酶变体及其应用
EP4028537A1 (fr) 2019-09-09 2022-07-20 DNA Script Synthèse de polynucléotides enzymatiques sans matrice à l'aide de liaisons photoclivables
JP2022549196A (ja) 2019-09-23 2022-11-24 ディーエヌエー スクリプト ポリヌクレオチドの鋳型なし酵素的合成における長配列収率の増加
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KR20240016237A (ko) * 2020-06-03 2024-02-06 몰레큘러 어셈블리즈, 인크. 폴리데옥시뉴클레오티드 합성을 위한 변형된 템플레이트-비의존성 효소
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10435676B2 (en) 2018-01-08 2019-10-08 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
US12286652B2 (en) 2018-01-08 2025-04-29 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
US11359221B2 (en) 2019-02-12 2022-06-14 Dna Script Sas Efficient product cleavage in template-free enzymatic synthesis of polynucleotides
US11905541B2 (en) 2019-02-12 2024-02-20 Dna Script Sas Efficient product cleavage in template-free enzymatic synthesis of polynucleotides

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