[go: up one dir, main page]

SG11201809961TA - Variants of a dna polymerase of the polx family - Google Patents

Variants of a dna polymerase of the polx family

Info

Publication number
SG11201809961TA
SG11201809961TA SG11201809961TA SG11201809961TA SG11201809961TA SG 11201809961T A SG11201809961T A SG 11201809961TA SG 11201809961T A SG11201809961T A SG 11201809961TA SG 11201809961T A SG11201809961T A SG 11201809961TA SG 11201809961T A SG11201809961T A SG 11201809961TA
Authority
SG
Singapore
Prior art keywords
variants
dna polymerase
polx family
nucleic acid
polx
Prior art date
Application number
SG11201809961TA
Inventor
Thomas Ybert
Marc Delarue
Original Assignee
Dna Script
Pasteur Institut
Centre Nat Rech Scient
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dna Script, Pasteur Institut, Centre Nat Rech Scient filed Critical Dna Script
Publication of SG11201809961TA publication Critical patent/SG11201809961TA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1264—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal nucleotidyl transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07031—DNA nucleotidylexotransferase (2.7.7.31), i.e. terminal deoxynucleotidyl transferase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

WO216472 PCT/FR2017/051519 54 ABTRACT The invention relates to variants of a DNA polymerase of the polX family capable of synthesizing a nucleic acid molecule without a template strand, or of a functional fragment of such a polymerase, comprising at least one mutatio n of a residue in at least one specific position, and to uses of said variants, in particular for the synthesis of nucleic acid molecules comprising 3’-OH modified nucleotides.
SG11201809961TA 2016-06-14 2017-06-13 Variants of a dna polymerase of the polx family SG11201809961TA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1655475A FR3052462A1 (en) 2016-06-14 2016-06-14 POLYMERASE DNA VARIANTS OF THE POLX FAMILY
PCT/FR2017/051519 WO2017216472A2 (en) 2016-06-14 2017-06-13 Variants of a dna polymerase of the polx family

Publications (1)

Publication Number Publication Date
SG11201809961TA true SG11201809961TA (en) 2018-12-28

Family

ID=57348782

Family Applications (2)

Application Number Title Priority Date Filing Date
SG11201809961TA SG11201809961TA (en) 2016-06-14 2017-06-13 Variants of a dna polymerase of the polx family
SG10202009688QA SG10202009688QA (en) 2016-06-14 2017-06-13 Variants of a dna polymerase of the polx family

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG10202009688QA SG10202009688QA (en) 2016-06-14 2017-06-13 Variants of a dna polymerase of the polx family

Country Status (11)

Country Link
US (3) US20200002690A1 (en)
EP (1) EP3469075A2 (en)
JP (2) JP7118510B2 (en)
KR (1) KR102522263B1 (en)
CN (1) CN109477080A (en)
AU (2) AU2017286477C1 (en)
CA (1) CA3024184A1 (en)
FR (2) FR3052462A1 (en)
IL (1) IL263503B2 (en)
SG (2) SG11201809961TA (en)
WO (1) WO2017216472A2 (en)

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10774316B2 (en) * 2014-10-20 2020-09-15 Molecular Assemblies, Inc. Modified template-independent enzymes for polydeoxynucleotide synthesis
JP2020521508A (en) 2017-05-26 2020-07-27 ヌクレラ ヌクレイクス リミテッド Use of terminal transferase enzymes in nucleic acid synthesis
WO2019030149A1 (en) 2017-08-07 2019-02-14 Dna Script Variants of family a dna polymerase and uses thereof
US10752887B2 (en) 2018-01-08 2020-08-25 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
AU2019205606A1 (en) * 2018-01-08 2020-07-30 Centre National De La Recherche Scientifique Variants of Terminal deoxynucleotidyl Transferase and uses thereof
CN113056327A (en) 2018-07-23 2021-06-29 Dna斯克瑞普特公司 Massively parallel enzymatic synthesis of nucleic acid strands
JP2022513031A (en) * 2018-11-14 2022-02-07 ディーエヌエー スクリプト Terminal deoxynucleotidyl transferase variant and its use
EP3894593B1 (en) 2018-12-13 2024-10-02 DNA Script Direct oligonucleotide synthesis on cdna
WO2020141143A1 (en) 2019-01-03 2020-07-09 Dna Script One pot synthesis of sets of oligonucleotides
GB201901501D0 (en) * 2019-02-04 2019-03-27 Nuclera Nucleics Ltd Modified terminal deoxynucleotidyl transferase (TdT) enzymes
WO2020165137A1 (en) 2019-02-12 2020-08-20 Dna Script Efficient product cleavage in template-free enzymatic synthesis of polynucleotides.
CN114207140A (en) * 2019-05-28 2022-03-18 Dna斯克瑞普特公司 Terminal deoxynucleotidyl transferase variants and uses thereof
EP3744854A1 (en) * 2019-05-28 2020-12-02 DNA Script Variants of terminal deoxynucleotidyl transferase and uses thereof
CA3145783A1 (en) 2019-07-30 2021-02-04 Tillmann HEINISCH Template-free enzymatic synthesis of polynucleotides using poly(a) and poly(u) polymerases
EP4007816A1 (en) 2019-08-01 2022-06-08 DNA Script Increasing long-sequence yields in template-free enzymatic synthesis of polynucleotides
CN110331136B (en) * 2019-09-05 2019-12-24 中国科学院天津工业生物技术研究所 A terminal deoxyribonucleoside transferase variant and its application
US20220315970A1 (en) 2019-09-09 2022-10-06 Dna Script Template-Free Enzymatic Polynucleotide Synthesis Using Photocleavable Linkages
CN114729389B (en) 2019-09-23 2024-03-08 Dna斯克瑞普特公司 Increasing long sequence yield in template-free enzymatic synthesis of polynucleotides
JP2023501651A (en) 2019-11-13 2023-01-18 ディーエヌエー スクリプト Highly efficient template-free enzymatic synthesis of polynucleotides
CN114787346A (en) * 2019-12-12 2022-07-22 Dna斯克瑞普特公司 Chimeric terminal deoxynucleotidyl transferase for template-free enzymatic synthesis of polynucleotides
CN115135770A (en) 2019-12-16 2022-09-30 Dna斯克瑞普特公司 Template-free enzymatic synthesis of polynucleotides using disproportionation-free terminal deoxynucleotidyl transferase variants
US20230089448A1 (en) 2020-02-25 2023-03-23 Dna Script Method And Apparatus for Enzymatic Synthesis of Polynucleotides
US20230159903A1 (en) 2020-04-20 2023-05-25 Dna Script Terminal Deoxynucleotidyl Transferase Variants and Uses Thereof
KR20230038415A (en) * 2020-05-12 2023-03-20 일루미나, 인코포레이티드 thermostable terminal deoxynucleotidyl transferase
WO2021231477A2 (en) 2020-05-12 2021-11-18 Illumina, Inc. Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase
JP2023528477A (en) * 2020-06-03 2023-07-04 モレキュラー アッセンブリーズ， インコーポレイテッド A modified template-independent enzyme for polydeoxynucleotide synthesis
EP4165175A2 (en) 2020-06-12 2023-04-19 Synhelix Ab-initio, template-independent synthesis of nucleic acids using thermostable enzymes
CA3182608A1 (en) 2020-06-16 2021-12-23 Carl Martin Systems, apparatus and kits for enzymatic polynucleotide synthesis
EP4182472A1 (en) 2020-07-15 2023-05-24 DNA Script Massively parallel enzymatic synthesis of polynucleotides
AU2021313713A1 (en) 2020-07-21 2023-01-05 Illumina Singapore Pte Ltd Base-modified nucleotides as substrates for tdt-based enzymatic nucleic acid
EP4192947A1 (en) * 2020-08-04 2023-06-14 Nuclera Nucleics Ltd Modified terminal deoxynucleotidyl transferase (tdt) enzymes
GB2598152B (en) * 2020-08-21 2025-04-16 Nuclera Ltd Modified terminal deoxynucleotidyl transferase (TdT) enzymes
CA3193386A1 (en) 2020-09-22 2022-03-31 Mikhael SOSKINE Stabilized n-terminally truncated terminal deoxynucleotidyl transferase variants and uses thereof
WO2022090323A1 (en) 2020-10-29 2022-05-05 Dna Script Enzymatic synthesis of polynucleotide probes
US12391970B2 (en) 2020-11-11 2025-08-19 Microsoft Technology Licensing, Llc Spatial control of polynucleotide synthesis by strand capping
CN112322715B (en) * 2020-11-17 2022-11-25 清华大学 A nucleic acid sequencing method
EP4314314A1 (en) 2021-04-02 2024-02-07 DNA Script Methods and kits for enzymatic synthesis of g4-prone polynucleotides
DE102022114351A1 (en) 2021-06-10 2022-12-15 Dna Script ENZYMATIC SYNTHESIS OF POLYNUCLEOTIDES USING 3'-O-AMINO-2'-DEOXYRIBONUCLEOSIDE TRIPHOSPHATE MONOMERS
EP4392554A1 (en) 2021-08-23 2024-07-03 DNA Script Method for generating double-stranded nucleic acid
US20240299945A1 (en) 2021-09-03 2024-09-12 Elegen Corporation Multi-way bead-sorting devices, systems, and methods of use thereof using pressure sources
EP4430175A2 (en) 2021-11-10 2024-09-18 DNA Script Novel terminal deoxynucleotidyl
US20250002879A1 (en) 2021-11-10 2025-01-02 Dna Script NOVEL TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE (TdT) VARIANTS
CN116555216A (en) * 2022-01-28 2023-08-08 中国科学院天津工业生物技术研究所 Terminal transferase variant for controllable synthesis of single-stranded DNA and application
WO2023170259A1 (en) 2022-03-11 2023-09-14 Dna Script Modular accessory rack
WO2023170286A2 (en) 2022-03-11 2023-09-14 Dna Script Alignment post and secure mechanism for enzymatic polynucleotide synthesis
WO2023170266A1 (en) 2022-03-11 2023-09-14 Dna Script Automation station for enzymatic polynucleotide synthesis
WO2023170258A1 (en) 2022-03-11 2023-09-14 Dna Script Apparatus for enzymatic synthesis of a plurality of polynucleotides comprising a condensation trap
WO2023240202A2 (en) * 2022-06-08 2023-12-14 Ansa Biotechnologies, Inc. Modified terminal deoxynucleotidyl transferase polymerases
WO2024013278A1 (en) 2022-07-13 2024-01-18 Dna Script Method of purifying solution comprising polynucleotide
EP4630540A1 (en) 2022-12-05 2025-10-15 DNA Script Variants of poly(a) polymerase and uses thereof
CN120359296A (en) 2022-12-09 2025-07-22 Dna斯克瑞普特公司 In vitro enzymatic RNA synthesis
EP4642920A1 (en) 2022-12-31 2025-11-05 DNA Script Variable viscosity inks for inkjet delivery of enzyme reagents
EP4652292A1 (en) 2023-01-16 2025-11-26 DNA Script Inkjet-assisted enzymatic nucleic acid synthesis
EP4652291A1 (en) 2023-01-16 2025-11-26 DNA Script Scarless template-free enzymatic synthesis of polynucleotides
EP4655411A2 (en) 2023-01-26 2025-12-03 DNA Script Enzymatic synthesis of polynucleotide probes
WO2024227846A2 (en) 2023-05-03 2024-11-07 Dna Script Reagent used in deprotection of 3'-o-amino polynucleotides
WO2024241324A1 (en) * 2023-05-24 2024-11-28 Scintium Ltd Method for training an artificial intelligence (ai) model and generating a 3d computer-aided design (cad) and system therefor
WO2024256604A1 (en) 2023-06-15 2024-12-19 Dna Script Salt addition during enzymatic polynucleotide synthesis
WO2025003434A1 (en) 2023-06-30 2025-01-02 Dna Script Nucleic acid synthesis on reusable support
WO2025021984A1 (en) 2023-07-27 2025-01-30 Dna Script Method for generating a plurality of polynucleotides
WO2025088024A1 (en) 2023-10-24 2025-05-01 Dna Script Novel terminal deoxynucleotidyl transferase (tdt) variants and uses thereof
WO2025093659A1 (en) 2023-11-04 2025-05-08 Dna Script 3'-o-aminooxymethyl blocked nucleotides and use thereof in enzymatic synthesis of polynucleotides
WO2025137872A1 (en) * 2023-12-26 2025-07-03 华大工程生物学长荡湖研究所 Terminal deoxyribonucleoside transferase mutant, preparation method therefor and use thereof
WO2025155239A1 (en) * 2024-01-17 2025-07-24 Agency For Science, Technology And Research X family dna polymerases and uses thereof
EP4653525A1 (en) * 2024-05-21 2025-11-26 Uab "Biomatter Designs" Engineered terminal deoxynucleotidyl transferase polymerases

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5004689A (en) 1982-02-22 1991-04-02 Biogen, Massachusetts DNA sequences, recombinant DNA molecules and processes for producing human gamma interferon-like polypeptides in high yields
IL71691A (en) 1984-04-27 1991-04-15 Yeda Res & Dev Production of interferon-ypsilon
US5047335A (en) 1988-12-21 1991-09-10 The Regents Of The University Of Calif. Process for controlling intracellular glycosylation of proteins
BG52073B2 (en) 1990-01-24 1996-04-30 Inst Molekuljarna Biolog Method for the preparation of recombinant human noncystein -interferon, free of n-end methionine
US6812339B1 (en) * 2000-09-08 2004-11-02 Applera Corporation Polymorphisms in known genes associated with human disease, methods of detection and uses thereof
WO2010062776A2 (en) * 2008-11-03 2010-06-03 Kapabiosystems Chimeric dna polymerases
US20120244209A1 (en) * 2011-03-02 2012-09-27 Roth Jack A Tusc2 therapies
EP3080158A4 (en) * 2013-12-09 2018-03-21 Adimab, LLC Polyclonal mixtures of antibodies, and methods of making and using them
FR3020071B1 (en) 2014-04-17 2017-12-22 Dna Script PROCESS FOR THE SYNTHESIS OF NUCLEIC ACIDS, IN PARTICULAR LARGE NUCLEIC ACIDS, USE OF THE METHOD AND KIT FOR IMPLEMENTING THE METHOD
FR3025201B1 (en) 2014-09-02 2018-10-12 Dna Script MODIFIED NUCLEOTIDES FOR THE SYNTHESIS OF NUCLEIC ACIDS, A KIT COMPRISING SUCH NUCLEOTIDES AND THEIR USE FOR GENERATING SYNTHETIC NUCLEIC ACID GENES OR SEQUENCES
EP3209781B8 (en) * 2014-10-20 2020-01-15 Molecular Assemblies Inc. Modified template-independent enzymes for polydeoxynucleotide synthesis

Also Published As

Publication number Publication date
AU2017286477C1 (en) 2023-12-21
AU2017286477A1 (en) 2018-12-06
EP3469075A2 (en) 2019-04-17
US20240124855A1 (en) 2024-04-18
JP2019517799A (en) 2019-06-27
US20200002690A1 (en) 2020-01-02
KR102522263B1 (en) 2023-04-18
WO2017216472A2 (en) 2017-12-21
AU2017286477B2 (en) 2023-09-14
SG10202009688QA (en) 2020-11-27
AU2023282219A1 (en) 2024-01-18
JP2022137127A (en) 2022-09-21
FR3053699B1 (en) 2021-04-02
IL263503B2 (en) 2025-04-01
FR3052462A1 (en) 2017-12-15
FR3053699A1 (en) 2018-01-12
IL263503A (en) 2019-02-03
CA3024184A1 (en) 2017-12-21
KR20190024883A (en) 2019-03-08
JP7118510B2 (en) 2022-08-16
CN109477080A (en) 2019-03-15
US20230167421A1 (en) 2023-06-01
IL263503B1 (en) 2024-12-01
WO2017216472A3 (en) 2018-03-15

Similar Documents

Publication Publication Date Title
SG11201809961TA (en) Variants of a dna polymerase of the polx family
WO2016106401A3 (en) Rna agents for p21 gene modulation
CA3096478A1 (en) Highly selective nucleic acid amplification primers
WO2015081229A3 (en) Selective amplification of nucleic acid sequences
MX2017002785A (en) Synthesis of dna.
EP4467654A3 (en) Genome fractioning
MX2019007248A (en) Polymerizing enzymes for sequencing reactions.
EP4520841A3 (en) Enzyme- and amplification-free sequencing
WO2016028803A3 (en) Methods and apparatus for synthesizing nucleic acids
WO2014162307A8 (en) Methods and apparatus for synthesizing nucleic acids
NZ744078A (en) Dna-encoded libraries having encoding oligonucleotide linkages not readable by polymerases
MX356792B (en) Plant regulatory elements and uses thereof.
EA038921B9 (en) Detection of target nucleic acid and variants thereof
WO2019122879A3 (en) Methods for performing biological reactions
WO2014210199A3 (en) Methods of performing polymerase chain reaction and related uses thereof
WO2016118726A3 (en) Methods and compositions for identification of highly specific nucleases
MX386318B (en) METHODS AND COMPOSITIONS FOR CHARACTERIZING DRUG-RESISTANT BACTERIA FROM FORMALIN-FIXED, PARAFFIN-EMBEDDED BIOLOGICAL SAMPLES.
HK1259060A1 (en) Single-stranded nucleic acid molecule inhibiting expression of prorenin gene or prorenin receptor gene, and use thereof
MX366113B (en) Diagnostic methods and compositions.
MX2018012545A (en) Reagents for treatment of oculopharyngeal muscular dystrophy (opmd) and use thereof.
WO2018011527A9 (en) Reagents for reversibly protecting biological molecules
WO2018009729A3 (en) Modification of dna polymerases for in vitro applications
EP3283501A4 (en) ST.VIOMETRIC ST REGULATION OF NUCLEIC ACID HYBRIDIZATION PROBES WITH AUXILIARY OLIGONUCLEOTIDE SPECIES
EP3350195A4 (en) PROCESS FOR SYNTHESIZING AND PURIFYING NUCLEOSIDE AND / OR NUCLEOTIDE, NUCLEOSIDE AND / OR MODIFIED NUCLEOTIDE (S), DNA MOLECULE AND OLIGONUCLEOTIDE LIBRARY COMPRISING THE SAME ) MODIFIED DIT (S) NUCLEOSIDE AND / OR NUCLEOTIDE (S) AND USE OF SAID OLIGONUCLEOTIDE LIBRARY
WO2018009726A3 (en) Modification of dna polymerases for in vitro applications