[go: up one dir, main page]

WO2010111344A3 - Methods and microorganisms for production of c4-dicarboxylic acids - Google Patents

Methods and microorganisms for production of c4-dicarboxylic acids Download PDF

Info

Publication number
WO2010111344A3
WO2010111344A3 PCT/US2010/028429 US2010028429W WO2010111344A3 WO 2010111344 A3 WO2010111344 A3 WO 2010111344A3 US 2010028429 W US2010028429 W US 2010028429W WO 2010111344 A3 WO2010111344 A3 WO 2010111344A3
Authority
WO
WIPO (PCT)
Prior art keywords
microorganisms
production
methods
dicarboxylic acids
pyruvate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2010/028429
Other languages
French (fr)
Other versions
WO2010111344A2 (en
Inventor
Adam Lawrence
Jacobus Thomas Pronk
Antonius Jeroen Adriaan Van Maris
Rintze Meindert Zelle
Kevin T. Madden
Jacob C. Harrison
Joshua Trueheart
Carlos Gancedo Rodriguez
Carmen-Lisset Flores Mauriz
Stanley Bower
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Microbia Inc
Original Assignee
Microbia Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Microbia Inc filed Critical Microbia Inc
Publication of WO2010111344A2 publication Critical patent/WO2010111344A2/en
Publication of WO2010111344A3 publication Critical patent/WO2010111344A3/en
Anticipated expiration legal-status Critical
Priority to IL215376A priority Critical patent/IL215376A0/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/38—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01037—Malate dehydrogenase (1.1.1.37)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/0104—Pyruvate kinase (2.7.1.40)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01001—Pyruvate decarboxylase (4.1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01031—Phosphoenolpyruvate carboxylase (4.1.1.31)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01032—Phosphoenolpyruvate carboxykinase (GTP) (4.1.1.32)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Methods and recombinant microorganisms for the biological production of C4-dicarboxylic acids are described. The recombinant microorganisms have increased production of OAA as compared to unmodified microorganisms. In some cases, the recombinant microorganisms have been modified to reduce production of pyruvate and/or reduce conversion of pyruvate to acetaldehyde. Thus, the microorganisms are modified to reduce the activity of pyruvate kinase and/or pyruvate decarboxylase.
PCT/US2010/028429 2009-03-24 2010-03-24 Methods and microorganisms for production of c4-dicarboxylic acids Ceased WO2010111344A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
IL215376A IL215376A0 (en) 2009-03-24 2011-09-25 Methods and microorganisms for production of c4-dicarboxylic acids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16301909P 2009-03-24 2009-03-24
US61/163,019 2009-03-24

Publications (2)

Publication Number Publication Date
WO2010111344A2 WO2010111344A2 (en) 2010-09-30
WO2010111344A3 true WO2010111344A3 (en) 2011-07-21

Family

ID=42781834

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/028429 Ceased WO2010111344A2 (en) 2009-03-24 2010-03-24 Methods and microorganisms for production of c4-dicarboxylic acids

Country Status (2)

Country Link
IL (1) IL215376A0 (en)
WO (1) WO2010111344A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2473600B1 (en) * 2009-09-01 2016-06-29 Novozymes Inc. Methods for improving malic acid production in filamentous fungi
CN103003298B (en) 2010-06-04 2016-02-10 诺维信股份有限公司 C4 dicarboxylic acid is produced in filamentous fungus
EP2582716A1 (en) 2010-06-21 2013-04-24 Novozymes, Inc. Aspergillus aculeatus derived polypeptides having c4-dicarboxylic acid transporter activity and polynucleotides encoding same
IN2013CN00459A (en) 2010-06-21 2015-07-03 Novozymes Inc
US9187772B2 (en) * 2010-09-01 2015-11-17 University Of Florida Research Foundation, Inc. L-malate production by metabolically engineered escherichia coli
EP2681307A1 (en) * 2011-02-28 2014-01-08 Novozymes, Inc. Microorganism for c4-dicarboxylic acid production
EP2495317A1 (en) * 2011-03-01 2012-09-05 Technische Universität Hamburg-Harburg Modified phosphoenolpyruvate carboxylase from Corynebacterium glutamicum and uses thereof
BR112014003444A2 (en) 2011-08-19 2017-03-14 Novozymes Inc recombinant host cell, and method for producing a c4-dicarboxylic acid
WO2013158749A2 (en) * 2012-04-17 2013-10-24 Gevo, Inc. Engineered microorganisms with improved growth properties
KR101426441B1 (en) 2012-05-16 2014-08-05 인하대학교 산학협력단 Recombinant microorganism expressing carbonic anhydrase from Dunaliella sp and phosphoenolpyruvate carboxylase from P.tricornutum CCMP 637, and method for producing organic acid using the same
CN112094826B (en) * 2013-12-12 2023-12-19 德希尼布能源法国公司 fumarate reductase
CN109415720B (en) * 2016-06-29 2021-11-26 花王株式会社 Method for producing C4 dicarboxylic acid
CN111051498A (en) * 2017-06-30 2020-04-21 齐米科技股份有限公司 Engineered Microorganisms for Enhanced Utilization of Oligosaccharides
US20240392307A1 (en) * 2019-10-15 2024-11-28 Donald Danforth Plant Science Center Methods and compositions for improving agronomic characteristics of c4 plants
CN113846024B (en) 2021-12-01 2022-03-29 南京昊禾生物科技有限公司 Method for reducing byproduct fumaric acid in L-malic acid fermentation process, strain and application

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009011974A1 (en) * 2007-05-18 2009-01-22 Microbia Precision Engineering, Inc. Organic acid production by fungal cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009011974A1 (en) * 2007-05-18 2009-01-22 Microbia Precision Engineering, Inc. Organic acid production by fungal cells

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CHRISTIAN RIEDEL ET AL: "Characterization of the Phosphoenolpyruvate Carboxykinase Gene from Corynebacterium glutamicum and Significance of the Enzyme for Growth and Amino Acid Production", J. MOL. MICROBIOL. BIOTECHNOL., vol. 3, no. 4, 2001, pages 573 - 583 *
HUI WU ET AL: "Improved Succinic Acid Production in the Anaerobic Culture of an Escherichia coli pflB IdhA Double Mutant as a Result of Enhanced Anaplerotic Activities in the Preceding Aerobic Culture", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 73, no. 24, 2007, pages 7837 - 7843 *
HYOHAK SONG ET AL: "Production of succinic acid by bacterial fermentation", ENZYME AND MICROBIAL TECHNOLOGY, vol. 39, no. ISS.3, 2006, pages 352 - 361 *
SANG JUN LEE ET AL: "Genome-Based Metabolic Engineering of Mannheimia succiniciproducens for Succinic Acid Production", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 72, no. 3, 2006, pages 1939 - 1948 *

Also Published As

Publication number Publication date
WO2010111344A2 (en) 2010-09-30
IL215376A0 (en) 2011-12-29

Similar Documents

Publication Publication Date Title
WO2010111344A3 (en) Methods and microorganisms for production of c4-dicarboxylic acids
WO2011142865A3 (en) Yeast microorganisms with reduced by-product accumulation for improved production of fuels, chemicals, and amino acids
WO2011003034A3 (en) Biological methods for preparing adipic acid
WO2011006136A3 (en) Engineered microorganisms with enhanced fermentation activity
WO2010014632A3 (en) Methods and compositions for improving the production of products in microorganisms
MY157111A (en) Recombinant microorganisms and methods of production thereof
WO2008073406A3 (en) Solvent tolerant microorganisms
WO2009132220A3 (en) Isoprene synthase variants for improved microbial production of isoprene
WO2011061032A3 (en) Cells, nucleic acids, enzymes, and use thereof, and methods for the production of sophorolipids
AU2012353654A8 (en) Modified microorganisms and methods of making butadiene using same
WO2011063308A8 (en) Beta-glucosidase i variants with improved properties
WO2012047733A3 (en) Treatment of acne by conditioned media
MY158663A (en) Fermentation broth formulations
EP2017344A8 (en) Production of itaconic acid
WO2012094425A3 (en) Biological methods for preparing adipic acid
WO2009049274A8 (en) Microorganism engineered to produce isopropanol
WO2012018699A3 (en) Improved fermentation process for the production of organic acids
MY182946A (en) Production of organic acids by fermentation at low ph
WO2012097091A3 (en) Engineered microorganisms with enhanced fermentation activity
WO2014099707A3 (en) Process for producing n-propanol and propionic acid using metabolically engineered propionibacteria
WO2012016177A3 (en) Genetically modified microbes producing increased levels of acetyl-coa derived compounds
WO2011072264A3 (en) Methods and compositions for biomass treatment
WO2012016172A3 (en) Genetically modified microbes producing increased levels of acetyl-coa derived compounds
WO2010099500A3 (en) Beta-glucosidase variant enzymes and related polynucleotides
WO2008063650A3 (en) Systems of hydrogen and formic acid production in yeast

Legal Events

Date Code Title Description
NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2012502193

Country of ref document: JP

NENP Non-entry into the national phase

Ref country code: JP

122 Ep: pct application non-entry in european phase

Ref document number: 10756761

Country of ref document: EP

Kind code of ref document: A2