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WO2012097091A3 - Engineered microorganisms with enhanced fermentation activity - Google Patents

Engineered microorganisms with enhanced fermentation activity Download PDF

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Publication number
WO2012097091A3
WO2012097091A3 PCT/US2012/020982 US2012020982W WO2012097091A3 WO 2012097091 A3 WO2012097091 A3 WO 2012097091A3 US 2012020982 W US2012020982 W US 2012020982W WO 2012097091 A3 WO2012097091 A3 WO 2012097091A3
Authority
WO
WIPO (PCT)
Prior art keywords
fermentation activity
engineered microorganisms
enhanced fermentation
microorganisms
enhanced
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2012/020982
Other languages
French (fr)
Other versions
WO2012097091A2 (en
Inventor
Kirsty Anne Lily SALMON
Jose Miguel Laplaza
Stephen Picataggio
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Verdezyne Inc
Original Assignee
Verdezyne Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Verdezyne Inc filed Critical Verdezyne Inc
Publication of WO2012097091A2 publication Critical patent/WO2012097091A2/en
Publication of WO2012097091A3 publication Critical patent/WO2012097091A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01005—Xylose isomerase (5.3.1.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Provided herein are genetically modified microorganisms that have enhanced fermentation activity, and methods for making and using such microorganisms.
PCT/US2012/020982 2011-01-12 2012-01-11 Engineered microorganisms with enhanced fermentation activity Ceased WO2012097091A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161432104P 2011-01-12 2011-01-12
US61/432,104 2011-01-12

Publications (2)

Publication Number Publication Date
WO2012097091A2 WO2012097091A2 (en) 2012-07-19
WO2012097091A3 true WO2012097091A3 (en) 2012-10-26

Family

ID=46507653

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/020982 Ceased WO2012097091A2 (en) 2011-01-12 2012-01-11 Engineered microorganisms with enhanced fermentation activity

Country Status (1)

Country Link
WO (1) WO2012097091A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9926347B2 (en) 2013-11-05 2018-03-27 Board Of Regents, The University Of Texas System Methods for engineering sugar transporter preferences

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011150313A1 (en) 2010-05-28 2011-12-01 Codexis, Inc. Pentose fermentation by a recombinant microorganism
WO2016016805A1 (en) 2014-08-01 2016-02-04 Versalis S.P.A. Gene construct for the transformation of yeast strains
US9920312B2 (en) 2014-08-11 2018-03-20 Lallemand Hungary Liquidity Management Llc Mutations in iron-sulfur cluster proteins that improve xylose utilization
US10619147B2 (en) * 2014-08-11 2020-04-14 Lallemand Hungary Liquidity Management Llc Chimeric polypeptides having xylose isomerase activity
US10724015B2 (en) * 2016-05-18 2020-07-28 Korea University Research And Business Foundation Microorganism having improved ability to produce N-acetylglucosamine as a result of modulating glycolytic flux
US11091753B2 (en) 2017-05-31 2021-08-17 Novozymes A/S Xylose fermenting yeast strains and processes thereof for ethanol production
EP3652301A1 (en) * 2017-07-11 2020-05-20 Adisseo France S.A.S. Enhanced metabolite-producing yeast
CN114107357B (en) * 2020-08-27 2024-04-05 中国科学院天津工业生物技术研究所 Recombinant filamentous fungus for producing ethanol by phosphofructokinase2 mutant, construction thereof and application of recombinant filamentous fungus in ethanol production
CN114592023B (en) * 2022-03-31 2023-03-24 杭州优玛达生物科技有限公司 Cell lysis self-assembly polypeptide compound, self-assembly method, self-assembly polypeptide preparation and application

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010039692A2 (en) * 2008-09-30 2010-04-08 The United States Of America, As Represented By The Secretary Of Agriculture Transformed saccharomyces cerevisiae engineered for xylose utilization

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010039692A2 (en) * 2008-09-30 2010-04-08 The United States Of America, As Represented By The Secretary Of Agriculture Transformed saccharomyces cerevisiae engineered for xylose utilization

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DATABASE NCBI 10 August 2010 (2010-08-10), "RecName: Full=Xylose isomerase", accession no. 9S306 *
DATABASE NCBI 15 April 2005 (2005-04-15), "Ruminococcus flavefaciens xylan utilization operon", accession no. J132472 *
KUYPER, M. ET AL.: "Evolutionary engineering of mixed-sugar utilization by a xylose-fermenting Saccharomyces cerevisiae strain", FEMS YEAST RESEARCH., vol. 5, no. 10, July 2005 (2005-07-01), pages 925 - 834, XP004967640, DOI: doi:10.1016/j.femsyr.2005.04.004 *
KUYPER, M. ET AL.: "HIgh-level functional expression of a fungal xylose isomerase: the key to efficient ethanolic fermentation of xylose by Saccharomyces cerevisiae?", FEMS YEAST RESEARCH., vol. 4, no. 1, October 2003 (2003-10-01), pages 69 - 78, XP002312913, DOI: doi:10.1016/S1567-1356(03)00141-7 *
MADHAVAN, A. ET AL.: "Alcoholic fermentation of xylose and mixed sugars using recombinant Saccharomyces cerevisiae engineered for xylose utilization", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY., vol. 82, no. 6, 6 June 2009 (2009-06-06), pages 1037 - 1047, XP019705462 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9926347B2 (en) 2013-11-05 2018-03-27 Board Of Regents, The University Of Texas System Methods for engineering sugar transporter preferences

Also Published As

Publication number Publication date
WO2012097091A2 (en) 2012-07-19

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