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WO2012016177A3 - Genetically modified microbes producing increased levels of acetyl-coa derived compounds - Google Patents

Genetically modified microbes producing increased levels of acetyl-coa derived compounds Download PDF

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Publication number
WO2012016177A3
WO2012016177A3 PCT/US2011/045953 US2011045953W WO2012016177A3 WO 2012016177 A3 WO2012016177 A3 WO 2012016177A3 US 2011045953 W US2011045953 W US 2011045953W WO 2012016177 A3 WO2012016177 A3 WO 2012016177A3
Authority
WO
WIPO (PCT)
Prior art keywords
acetyl
genetically modified
derived compounds
increased levels
coa derived
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2011/045953
Other languages
French (fr)
Other versions
WO2012016177A2 (en
Inventor
Darren Platt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amyris Inc
Original Assignee
Amyris Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amyris Inc filed Critical Amyris Inc
Publication of WO2012016177A2 publication Critical patent/WO2012016177A2/en
Publication of WO2012016177A3 publication Critical patent/WO2012016177A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y306/00—Hydrolases acting on acid anhydrides (3.6)
    • C12Y306/04—Hydrolases acting on acid anhydrides (3.6) acting on acid anhydrides; involved in cellular and subcellular movement (3.6.4)
    • C12Y306/04009—Chaperonin ATPase (3.6.4.9)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Provided herein are genetically modified microorganisms that produce increased amounts of acetyl-CoA derived compounds in industrial fermentation processes, and methods of making and using the same.
PCT/US2011/045953 2010-07-30 2011-07-29 Genetically modified microbes producing increased levels of acetyl-coa derived compounds Ceased WO2012016177A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36955110P 2010-07-30 2010-07-30
US61/369,551 2010-07-30

Publications (2)

Publication Number Publication Date
WO2012016177A2 WO2012016177A2 (en) 2012-02-02
WO2012016177A3 true WO2012016177A3 (en) 2012-05-10

Family

ID=45530751

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/045953 Ceased WO2012016177A2 (en) 2010-07-30 2011-07-29 Genetically modified microbes producing increased levels of acetyl-coa derived compounds

Country Status (1)

Country Link
WO (1) WO2012016177A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013044475A1 (en) * 2011-09-29 2013-04-04 私立辅仁大学 Method and system utilizing cystofilobasidium genus of yeast to produce grease
US20170298363A1 (en) 2014-09-18 2017-10-19 Genomatica, Inc Non-natural microbial organisms with improved energetic efficiency
JP2021505154A (en) 2017-12-07 2021-02-18 ザイマージェン インコーポレイテッド Designed biosynthetic pathway for producing (6E) -8-hydroxygeraniol by fermentation
WO2019126778A1 (en) 2017-12-21 2019-06-27 Zymergen Inc. Nepetalactol oxidoreductases, nepetalactol synthases, and microbes capable of producing nepetalactone
CN110656056B (en) * 2019-10-31 2021-07-27 江南大学 A kind of construction method of high-concentration pinene-tolerant pinene-producing engineering bacteria
CN110951631B (en) * 2019-12-31 2021-03-02 江南大学 A geraniol-producing grape juice Hansenula spp. and its fermentation method
CN111500606A (en) * 2020-05-06 2020-08-07 浙江大学 Gene participating in biosynthesis of linalool from peach trees and application of gene

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100159546A1 (en) * 2007-03-30 2010-06-24 Aristos Aristidou Metabolic engineering of yeasts for the production of 1-butanol

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100159546A1 (en) * 2007-03-30 2010-06-24 Aristos Aristidou Metabolic engineering of yeasts for the production of 1-butanol

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
BOSL, B. ET AL.: "The molecular chaperone Hsp104-A molecular machine for protein disaggregation.", JOURNAL OF STRUCTURAL BIOLOGY., vol. 156, 6 March 2006 (2006-03-06), pages 139 - 148 *
ERIKSSON ET AL.: "The Escherichia coli heat shock protein ClpB restores acquired thermotolerance to a cyanobacterial clpB deletion mutant", CELL STRESS & CHAPERONES., vol. 5, no. 3, 2000, pages 255 - 264 *
HATTENDORF ET AL.: "Cooperative kinetics of both Hsp104 ATPase domains and interdomain communication revealed by AAA sensor-1 mutants", EMBO J., vol. 21, no. 1, 2, 2002, pages 12 - 21 *
PARSELL ET AL.: "Saccharomyces cerevisiue Hsp104 Protein", J. BIOL. CHEM., vol. 269, no. 6, 11 February 1994 (1994-02-11), pages 4480 - 4487 *
PARSELL, D. A. ET AL., UNIPROT SUBMISSION ACCESSION P31539, 1 July 1993 (1993-07-01), pages 1, Retrieved from the Internet <URL:http://www.uniprot.org/uniproUP31539.txt?version=1> [retrieved on 20111208] *
PISZCZEK ET AL.: "The Molecular Chaperone, ClpA, Has a Single High Affinity Peptide Binding Site per Hexamer", J. BIOL CHEM., vol. 280, no. 13, 1 April 2005 (2005-04-01), pages 12221 - 12230 *
SCHIRMER ET AL.: "Subunit interactions influence the biochemical and biological properties of Hsp104", PNAS., vol. 98, no. 3, 30 January 2001 (2001-01-30), pages 914 - 919 *
SCHIRMER ET AL.: "The ATPase Activity of Hsp104, Effects of Environmental Conditions and Mutations", J. BIOL CHEM., vol. 273, no. 25, 19 June 1998 (1998-06-19), pages 15546 - 15552 *
TAKAHASHI ET AL.: "A Systematic Evaluation of the Function of the Protein-Remodeling Factor Hsp104 in [PSI+] Prion Propagation in S. cerevisiae by Comprehensive Chromosomal Mutations", PRION., vol. 1, no. 1, 2007, pages 69 - 77 *

Also Published As

Publication number Publication date
WO2012016177A2 (en) 2012-02-02

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