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WO2009038195A1 - 変異体ピロリジル－ｔＲＮＡ合成酵素及びこれを用いる非天然アミノ酸組み込みタンパク質の製造方法 - Google Patents

変異体ピロリジル－ｔＲＮＡ合成酵素及びこれを用いる非天然アミノ酸組み込みタンパク質の製造方法 Download PDF

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WO2009038195A1
WO2009038195A1 PCT/JP2008/067029 JP2008067029W WO2009038195A1 WO 2009038195 A1 WO2009038195 A1 WO 2009038195A1 JP 2008067029 W JP2008067029 W JP 2008067029W WO 2009038195 A1 WO2009038195 A1 WO 2009038195A1
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residue
amino acid
substitution
natural amino
protein
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French (fr)
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Shigeyuki Yokoyama
Kensaku Sakamoto
Tatsuo Yanagisawa
Takatsugu Kobayashi
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RIKEN
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Priority to AU2008301614A priority Critical patent/AU2008301614A1/en
Priority to CA2706889A priority patent/CA2706889C/en
Priority to BRPI0817212-9A2A priority patent/BRPI0817212A2/pt
Priority to CN2008801078970A priority patent/CN101952428B/zh
Priority to EP08832011.4A priority patent/EP2192185B1/en
Priority to JP2009533208A priority patent/JP5590649B2/ja
Publication of WO2009038195A1 publication Critical patent/WO2009038195A1/ja
Priority to US12/727,037 priority patent/US8735093B2/en
Anticipated expiration legal-status Critical
Priority to US14/248,062 priority patent/US9133449B2/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
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  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
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  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

　重原子、セレン、反応性官能基、蛍光基、クロスリンカー等、有用な官能基を有する非天然アミノ酸として好適なリジン誘導体、特にＮε－ベンジルオキシカルボニル－リジン（Ｚ－Ｌｙｓ）誘導体を所望のタンパク質に部位特異的に導入する方法を提供する。 　配列番号２に示したピロリジル－ｔＲＮＡ合成酵素のアミノ酸配列においてピロリジン結合部位を構成する３０６位のチロシン、３０９位のロイシン、及び３４８位のシステインから選択される少なくとも１つのアミノ酸残基が置換された変異体酵素であって、当該アミノ酸置換が、 　３０６位のチロシンからグリシン又はアラニンへ、 　３０９位のロイシンからグリシン又はアラニンへ、及び 　３４８位のシステインからバリン、セリン又はアラニンへ、 　の置換である変異体ピロリジル－ｔＲＮＡ合成酵素。
PCT/JP2008/067029 2007-09-20 2008-09-19 変異体ピロリジル－ｔＲＮＡ合成酵素及びこれを用いる非天然アミノ酸組み込みタンパク質の製造方法 Ceased WO2009038195A1 (ja)

Priority Applications (8)

Application Number Priority Date Filing Date Title
AU2008301614A AU2008301614A1 (en) 2007-09-20 2008-09-19 Mutant pyrrolysyl-TRNA synthetase, and method for production of protein having non-natural amino acid integrated therein by using the same
CA2706889A CA2706889C (en) 2007-09-20 2008-09-19 Mutant pyrrolysyl-trna synthetase, and method for production of protein having non-natural amino acid integrated therein by using the same
BRPI0817212-9A2A BRPI0817212A2 (pt) 2007-09-20 2008-09-19 Pirrolisil-trna sintetase mutante, e método para a produção de proteína tendo aminoácido não-natural integrado nela por sua utilização
CN2008801078970A CN101952428B (zh) 2007-09-20 2008-09-19 突变体吡咯赖氨酰-tRNA合成酶以及使用该酶的非天然氨基酸整合蛋白质的制造方法
EP08832011.4A EP2192185B1 (en) 2007-09-20 2008-09-19 Mutant pyrrolysyl-trna synthetase, and method for production of protein having non-natural amino acid integrated therein by using the same
JP2009533208A JP5590649B2 (ja) 2007-09-20 2008-09-19 変異体ピロリジル−ｔＲＮＡ合成酵素及びこれを用いる非天然アミノ酸組み込みタンパク質の製造方法
US12/727,037 US8735093B2 (en) 2007-09-20 2010-03-18 Mutant pyrrolysyl-tRNA synthetase, and method for production of protein having non-natural amino acid integrated therein by using the same
US14/248,062 US9133449B2 (en) 2007-09-20 2014-04-08 Mutant pyrrolysyl-tRNA synthetase, and method for production of protein having non-natural amino acid integrated therein by using the same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007243574 2007-09-20
JP2007-243574 2007-09-20

Related Child Applications (1)

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US12/727,037 Continuation US8735093B2 (en) 2007-09-20 2010-03-18 Mutant pyrrolysyl-tRNA synthetase, and method for production of protein having non-natural amino acid integrated therein by using the same

Publications (1)

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WO2009038195A1 true WO2009038195A1 (ja) 2009-03-26

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PCT/JP2008/067029 Ceased WO2009038195A1 (ja) 2007-09-20 2008-09-19 変異体ピロリジル－ｔＲＮＡ合成酵素及びこれを用いる非天然アミノ酸組み込みタンパク質の製造方法

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US (2) US8735093B2 (ja)
EP (1) EP2192185B1 (ja)
JP (1) JP5590649B2 (ja)
CN (1) CN101952428B (ja)
AU (1) AU2008301614A1 (ja)
BR (1) BRPI0817212A2 (ja)
CA (1) CA2706889C (ja)
WO (1) WO2009038195A1 (ja)

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CN102504022A (zh) * 2011-11-30 2012-06-20 苏州元基生物技术有限公司 含有保护赖氨酸的胰岛素原及使用其制备胰岛素的方法
JP2013107842A (ja) * 2011-11-18 2013-06-06 Institute Of Physical & Chemical Research 新規な非天然型アミノ酸、及びその利用
JP2013522352A (ja) * 2010-03-24 2013-06-13 メディカル リサーチ カウンシル 方法
WO2014044872A1 (en) 2012-09-24 2014-03-27 Allozyne, Inc Cell lines
JP2016193925A (ja) * 2011-02-03 2016-11-17 エー エム ベー エル シクロオクチニル又はトランス−シクロオクテニル類似基を含む非天然型アミノ酸及びその使用
WO2017030156A1 (ja) * 2015-08-19 2017-02-23 国立研究開発法人理化学研究所 非天然アミノ酸導入抗体
JPWO2016148044A1 (ja) * 2015-03-13 2017-12-28 中外製薬株式会社 改変アミノアシルｔＲＮＡ合成酵素およびその用途
WO2020045656A1 (ja) 2018-08-31 2020-03-05 国立研究開発法人理化学研究所 ピロリシルｔＲＮＡ合成酵素
CN111051512A (zh) * 2017-07-11 2020-04-21 辛索克斯公司 非天然核苷酸的掺入及其方法
JP2021136997A (ja) * 2020-03-06 2021-09-16 国立研究開発法人農業・食品産業技術総合研究機構 カウンターセレクションマーカー
JP2022527991A (ja) * 2019-03-19 2022-06-07 ニンポー クンペン バイオテック カンパニー，リミテッド タンパク質に効率的にリジン誘導体を導入するアミノアシル－ｔＲＮＡ合成酵素
JP2022549931A (ja) * 2019-09-30 2022-11-29 ザ スクリプス リサーチ インスティテュート 真核生物の半合成生物
CN115701451A (zh) * 2021-08-02 2023-02-10 宁波鲲鹏生物科技有限公司 高效引入赖氨酸衍生物的氨酰基-tRNA合成酶及其应用

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5590649B2 (ja) 2007-09-20 2014-09-17 独立行政法人理化学研究所 変異体ピロリジル−ｔＲＮＡ合成酵素及びこれを用いる非天然アミノ酸組み込みタンパク質の製造方法
WO2009066761A1 (ja) * 2007-11-22 2009-05-28 Riken エステル結合を含む非天然タンパク質の製造方法
CN102838663B (zh) * 2011-06-23 2014-06-11 北京大学 定点突变和定点修饰的病毒膜蛋白、其制备方法及其应用
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US10131920B2 (en) * 2013-03-15 2018-11-20 Medimmune Limited Nucleic acid molecules
JP6479022B2 (ja) 2014-01-14 2019-03-06 ヨーロピアン モレキュラー バイオロジー ラボラトリーＥｕｒｏｐｅａｎ Ｍｏｌｅｃｕｌａｒ Ｂｉｏｌｏｇｙ Ｌａｂｏｒａｔｏｒｙ 分子標識のための複数の環化付加反応
GB2528227A (en) 2014-03-14 2016-01-20 Medical Res Council Cyclopropene amino acids and methods
WO2015193897A1 (en) * 2014-06-17 2015-12-23 B G Negev Technologies And Applications Ltd At Ben Genetically expanded cell free protein synthesis systems, methods and kits
GB201419109D0 (en) 2014-10-27 2014-12-10 Medical Res Council Incorporation of unnatural amino acids into proteins
CN105541962B (zh) * 2015-12-19 2021-04-02 深圳市贝沃德克生物技术研究院有限公司 基于7-羟基香豆素的定点荧光标记方法
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US11884612B2 (en) 2016-03-16 2024-01-30 The Regents Of The University Of California Covalent peptide binders
WO2018044143A1 (ko) * 2016-09-05 2018-03-08 한국과학기술원 목적 단백질의 위치 특이적 변형이 시공간적으로 조절되는 마우스, 이의 제조방법 및 그 용도
EP3309260A1 (en) 2016-10-14 2018-04-18 European Molecular Biology Laboratory Archaeal pyrrolysyl trna synthetases for orthogonal use
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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004070024A1 (ja) 2003-02-10 2004-08-19 Riken チロシルtRNA合成酵素の変異体及びその作製方法
JP2007037445A (ja) 2005-08-02 2007-02-15 Institute Of Physical & Chemical Research ｔＲＮＡ合成方法、核酸、アミノアシルｔＲＮＡ合成方法及び非天然型アミノ酸組み込み蛋白質の製造方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS625942A (ja) * 1985-07-02 1987-01-12 Toray Ind Inc Ｌ−またはｄ−ｎ２−ベンジルオキシカルボニルリジンの製造方法
US20060166319A1 (en) * 2004-08-13 2006-07-27 Chan Michael K Charging tRNA with pyrrolysine
US20060183888A1 (en) * 2004-08-13 2006-08-17 Chan Michael K Pyrrolysine synthesis and modification
JP5196378B2 (ja) 2006-02-22 2013-05-15 独立行政法人理化学研究所 サプレッサーｔＲＮＡの合成方法、ＤＮＡ構築物及びそれを用いた非天然型アミノ酸組み込みタンパク質の製造
JP5590649B2 (ja) 2007-09-20 2014-09-17 独立行政法人理化学研究所 変異体ピロリジル−ｔＲＮＡ合成酵素及びこれを用いる非天然アミノ酸組み込みタンパク質の製造方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004070024A1 (ja) 2003-02-10 2004-08-19 Riken チロシルtRNA合成酵素の変異体及びその作製方法
JP2007037445A (ja) 2005-08-02 2007-02-15 Institute Of Physical & Chemical Research ｔＲＮＡ合成方法、核酸、アミノアシルｔＲＮＡ合成方法及び非天然型アミノ酸組み込み蛋白質の製造方法

Non-Patent Citations (18)

* Cited by examiner, † Cited by third party
Title
BLIGHT S.K. ET AL., NATURE, vol. 431, 2004, pages 333 - 335
COHEN, S.N. ET AL., PROC. NATL. ACAD. SCI., USA, vol. 69, 1972, pages 2110 - 2114
KAVRAN, J. M. ET AL.: "Structure of pyrrolysyl- tRNA synthetase, an archaeal enzyme for genetic code innovation", PROC. NATL. ACAD. SCI. USA, vol. 104, no. 27, July 2007 (2007-07-01), pages 11268 - 11273, XP002610072 *
KAVRAN, J.M. ET AL., PROC. NATL. ACAD. SCI., vol. 104, 2007, pages 11268 - 11273
KOBAYASHI, T. ET AL., IN VIVO SITE-SPECIFIC INTRODUCTION OF NON-NATURAL MAIN-CHAIN/SIDE- CHAIN STRUCTURES INTO PROTEINS BY USING PRIMITIVE AMINOACYL-TRNA SYNTHETASES, December 2007 (2007-12-01), SHOROKU CD, 4P-0337 *
LIU, W. ET AL., NAT. METHODS., vol. 4, 2007, pages 239 - 244
MUKAI ET AL., BIOCHEM. BIOPHYS. RES. COMMUN., vol. 371, 2008, pages 818 - 822
MUKAI, T. ET AL.: "Adding L-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases", BIOCHEM. BIOPHYS. RES. COMMUN., vol. 371, May 2008 (2008-05-01), pages 818 - 822, XP022688470 *
OHNO, S. ET AL., J. BIOCHEM. (TOKYO), vol. 141, 2007, pages 335 - 343
POLYCARPO, C. R. ET AL.: "Pyrrolysine analogues as substrates for pyrrolysyl-tRNA synthetase", FEBS LETTERS, vol. 580, 2006, pages 6695 - 6700, XP025232841 *
SAKAMOTO, K. ET AL., NUCLEIC ACIDS RESEARCH, vol. 30, 2002, pages 4692 - 4699
See also references of EP2192185A4 *
TSAO, M.-L.; TIAN, F.; SCHULTZ, P. G., CHEMBIOCHEM, vol. 2005, no. 6, pages 2147 - 2149
YANAGISAWA, T. ET AL., ACTA CRYST., vol. F62, 2006, pages 1031 - 1033
YANAGISAWA, T. ET AL.: "Crystallographic studies on multiple conformational states of active-site loops in pyrrolysyl-tRNA synthetase", J. MOL. BIOL., vol. 378, February 2008 (2008-02-01), pages 634 - 652, XP022762540 *
YANAGISAWA, T. ET AL.: "Site-specific incorporation of unnatural amino acids into protein by using pyrrolysyl-tRNA synthetase", RNA MEETING, vol. 8, 2006, pages 1 *
ZHANG, Y. ET AL.: "Crystal structures of apo wild-type M. jannaschii tyrosyl-tRNA synthetase (TyrRS) and an engineered TyrRS specific for O-methyl-L-tyrosine", PROTEIN SCIENCE, vol. 14, 2005, pages 1340 - 1349, XP008132347 *
ZUBAY ET AL., ANN. REV. GENET., vol. 7, 1973, pages 267 - 287

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JP2016193925A (ja) * 2011-02-03 2016-11-17 エー エム ベー エル シクロオクチニル又はトランス−シクロオクテニル類似基を含む非天然型アミノ酸及びその使用
JP2013107842A (ja) * 2011-11-18 2013-06-06 Institute Of Physical & Chemical Research 新規な非天然型アミノ酸、及びその利用
CN102504022A (zh) * 2011-11-30 2012-06-20 苏州元基生物技术有限公司 含有保护赖氨酸的胰岛素原及使用其制备胰岛素的方法
WO2014044872A1 (en) 2012-09-24 2014-03-27 Allozyne, Inc Cell lines
JP7020910B2 (ja) 2015-03-13 2022-02-16 中外製薬株式会社 改変アミノアシルｔＲＮＡ合成酵素およびその用途
JPWO2016148044A1 (ja) * 2015-03-13 2017-12-28 中外製薬株式会社 改変アミノアシルｔＲＮＡ合成酵素およびその用途
WO2017030156A1 (ja) * 2015-08-19 2017-02-23 国立研究開発法人理化学研究所 非天然アミノ酸導入抗体
US10906990B2 (en) 2015-08-19 2021-02-02 Riken Antibody with non-natural amino acid introduced therein
US11787877B2 (en) 2015-08-19 2023-10-17 Riken Antibody with non-natural amino acid introduced therein
JPWO2017030156A1 (ja) * 2015-08-19 2018-06-07 国立研究開発法人理化学研究所 非天然アミノ酸導入抗体
US12319944B2 (en) 2017-07-11 2025-06-03 The Scripps Research Institute Incorporation of unnatural nucleotides and methods thereof
EP3652316A4 (en) * 2017-07-11 2021-04-07 Synthorx, Inc. INCORPORATION OF NON-NATURAL NUCLEOTIDES AND RELATED PROCESSES
JP2020526197A (ja) * 2017-07-11 2020-08-31 シンソークス，インク． 非天然ヌクレオチドの組み込み及びその方法
JP7645942B2 (ja) 2017-07-11 2025-03-14 シンソークス，インク． 非天然ヌクレオチドの組み込み及びその方法
JP2023175678A (ja) * 2017-07-11 2023-12-12 シンソークス，インク． 非天然ヌクレオチドの組み込み及びその方法
JP7325341B2 (ja) 2017-07-11 2023-08-14 シンソークス，インク． 非天然ヌクレオチドの組み込み及びその方法
CN111051512A (zh) * 2017-07-11 2020-04-21 辛索克斯公司 非天然核苷酸的掺入及其方法
US11834689B2 (en) 2017-07-11 2023-12-05 The Scripps Research Institute Incorporation of unnatural nucleotides and methods thereof
JPWO2020045656A1 (ja) * 2018-08-31 2021-08-12 国立研究開発法人理化学研究所 ピロリシルｔＲＮＡ合成酵素
WO2020045656A1 (ja) 2018-08-31 2020-03-05 国立研究開発法人理化学研究所 ピロリシルｔＲＮＡ合成酵素
JP7667544B2 (ja) 2018-08-31 2025-04-23 国立研究開発法人理化学研究所 ピロリシルｔＲＮＡ合成酵素
JP7383306B2 (ja) 2019-03-19 2023-11-20 ニンポー クンペン バイオテック カンパニー，リミテッド タンパク質に効率的にリジン誘導体を導入するアミノアシル－ｔＲＮＡ合成酵素
JP2022527991A (ja) * 2019-03-19 2022-06-07 ニンポー クンペン バイオテック カンパニー，リミテッド タンパク質に効率的にリジン誘導体を導入するアミノアシル－ｔＲＮＡ合成酵素
JP2022549931A (ja) * 2019-09-30 2022-11-29 ザ スクリプス リサーチ インスティテュート 真核生物の半合成生物
JP7692900B2 (ja) 2019-09-30 2025-06-16 ザ スクリプス リサーチ インスティテュート 真核生物の半合成生物
JP7521804B2 (ja) 2020-03-06 2024-07-24 国立研究開発法人農業・食品産業技術総合研究機構 カウンターセレクションマーカー
JP2021136997A (ja) * 2020-03-06 2021-09-16 国立研究開発法人農業・食品産業技術総合研究機構 カウンターセレクションマーカー
CN115701451A (zh) * 2021-08-02 2023-02-10 宁波鲲鹏生物科技有限公司 高效引入赖氨酸衍生物的氨酰基-tRNA合成酶及其应用

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