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WO2003008588A3 - Method for producing 2-keto-l-gulonic acid and vitamin c while using l-sorbose dehydrogenase and l-sorbosone dehydrogenase - Google Patents

Method for producing 2-keto-l-gulonic acid and vitamin c while using l-sorbose dehydrogenase and l-sorbosone dehydrogenase Download PDF

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Publication number
WO2003008588A3
WO2003008588A3 PCT/EP2002/007484 EP0207484W WO03008588A3 WO 2003008588 A3 WO2003008588 A3 WO 2003008588A3 EP 0207484 W EP0207484 W EP 0207484W WO 03008588 A3 WO03008588 A3 WO 03008588A3
Authority
WO
WIPO (PCT)
Prior art keywords
dehydrogenase
keto
producing
vitamin
sorbosone
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2002/007484
Other languages
German (de)
French (fr)
Other versions
WO2003008588A2 (en
Inventor
Knut Eichler
Christine Beck
Thomas Friedrich
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF SE
Original Assignee
BASF SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BASF SE filed Critical BASF SE
Priority to AU2002328843A priority Critical patent/AU2002328843A1/en
Publication of WO2003008588A2 publication Critical patent/WO2003008588A2/en
Publication of WO2003008588A3 publication Critical patent/WO2003008588A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14—Pretreatment of feeding-stuffs with enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/58—Aldonic, ketoaldonic or saccharic acids
    • C12P7/60—2-Ketogulonic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/99—Oxidoreductases acting on the CH-OH group of donors (1.1) with other acceptors (1.1.99)
    • C12Y101/99012—Sorbose dehydrogenase (1.1.99.12)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Physiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to polypeptides having an L-sorbose dehydrogenase (SDH) or L-sorbosone dehydrogenase (SNDH) activity and nucleic acid sequences that code for these polypeptides. The invention also relates to transgenic expression contructs, vectors and transgenic organisms containing these nucleic acid sequences, and to methods for producing 2-keto-L-gulonic acid or ascorbic acid while using the same.
PCT/EP2002/007484 2001-07-13 2002-07-05 Method for producing 2-keto-l-gulonic acid and vitamin c while using l-sorbose dehydrogenase and l-sorbosone dehydrogenase Ceased WO2003008588A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002328843A AU2002328843A1 (en) 2001-07-13 2002-07-05 Method for producing 2-keto-l-gulonic acid and vitamin c while using l-sorbose dehydrogenase and l-sorbosone dehydrogenase

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10133397 2001-07-13
DE10133397.8 2001-07-13

Publications (2)

Publication Number Publication Date
WO2003008588A2 WO2003008588A2 (en) 2003-01-30
WO2003008588A3 true WO2003008588A3 (en) 2003-11-06

Family

ID=7691222

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/007484 Ceased WO2003008588A2 (en) 2001-07-13 2002-07-05 Method for producing 2-keto-l-gulonic acid and vitamin c while using l-sorbose dehydrogenase and l-sorbosone dehydrogenase

Country Status (2)

Country Link
AU (1) AU2002328843A1 (en)
WO (1) WO2003008588A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100395332C (en) * 2003-11-28 2008-06-18 华北制药集团有限责任公司 A kind of L-sorbosone dehydrogenase and its coding gene and application
WO2006084720A2 (en) * 2005-02-11 2006-08-17 Dsm Ip Assets B.V. Hpr kinase from gluconobacter oxydan
ES2372879T3 (en) 2006-06-22 2012-01-27 Ikeda Food Research Co. Ltd. PROCEDURE FOR DETERMINING 1,5-ANHYDROGLUCITOL AND REACTIVE COMPOSITION TO DETERMINE 1,5-ANHYDROGLUCITOL.
CN102653767A (en) * 2012-05-26 2012-09-05 江南大学 Novel gene for L-sorbose/L-sorbosone dehydrogenase and application of novel gene
CN103254159A (en) * 2013-01-29 2013-08-21 南京凯通粮食生化研究设计有限公司 Vitamin C production process improvement method

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0276832A2 (en) * 1987-01-30 1988-08-03 F. Hoffmann-La Roche Ag Process for the preparation of cetogulonic acid
EP0753575A1 (en) * 1993-03-08 1997-01-15 Fujisawa Pharmaceutical Co., Ltd. NOVEL L-SORBOSE DEHYDROGENASE AND NOVEL L-SORBOSONE DEHYDROGENASE OBTAINED FROM $i(GLUCONOBACTER OXYDANS) T-100
EP0758679A1 (en) * 1994-02-25 1997-02-19 Fujisawa Pharmaceutical Co., Ltd. Process for producing 2-keto-l-gulonic acid

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0276832A2 (en) * 1987-01-30 1988-08-03 F. Hoffmann-La Roche Ag Process for the preparation of cetogulonic acid
EP0753575A1 (en) * 1993-03-08 1997-01-15 Fujisawa Pharmaceutical Co., Ltd. NOVEL L-SORBOSE DEHYDROGENASE AND NOVEL L-SORBOSONE DEHYDROGENASE OBTAINED FROM $i(GLUCONOBACTER OXYDANS) T-100
EP0758679A1 (en) * 1994-02-25 1997-02-19 Fujisawa Pharmaceutical Co., Ltd. Process for producing 2-keto-l-gulonic acid

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ASAKURA A ET AL: "ISOLATION AND CHARACTERIZATION OF A NEW QUINOPROTEIN DEHYDROGENASE, L-SORBOSE/L-SORBOSONE DEHYDROGENASE", BIOSCIENCE BIOTECHNOLOGY BIOCHEMISTRY, JAPAN SOC. FOR BIOSCIENCE, BIOTECHNOLOGY AND AGROCHEM. TOKYO, JP, vol. 63, no. 1, January 1999 (1999-01-01), pages 46 - 53, XP001085040, ISSN: 0916-8451 *
LEE H -W ET AL: "Screening for L-sorbose and L-sorbosone dehydrogenase producing microbes for 2-keto-L-gulonic acid production.", JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, vol. 23, no. 2, August 1999 (1999-08-01), pages 106 - 111, XP002241676, ISSN: 1367-5435 *
SAITO Y ET AL: "CLONING OF GENES CODING FOR L-SORBOSE AND L-SORBOSONE DEHYDROGENASES FROM GLUCONOBACTER OXYDANS AND MICROBIAL PRODUCTION OF 2-KETO-L-GULONATE, A PRECURSOR OF L-ASCORBIC ACID, IN A RECOMBINANT G. OXYDANS STRAIN", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 63, no. 2, 1997, pages 454 - 460, XP000886144, ISSN: 0099-2240 *

Also Published As

Publication number Publication date
WO2003008588A2 (en) 2003-01-30
AU2002328843A1 (en) 2003-03-03

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