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US7851675B2 - Stress resistant plants - Google Patents

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US7851675B2
US7851675B2 US11/663,657 US66365705A US7851675B2 US 7851675 B2 US7851675 B2 US 7851675B2 US 66365705 A US66365705 A US 66365705A US 7851675 B2 US7851675 B2 US 7851675B2
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plants
chimeric gene
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Marc De Block
Michael Metzlaff
Veronique Gossele
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BASF SE
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1077—Pentosyltransferases (2.4.2)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
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    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07018—Nicotinate-nucleotide adenylyltransferase (2.7.7.18)
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    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
    • C12Y305/01019—Nicotinamidase (3.5.1.19)

Definitions

  • Methods are provided for increasing the stress resistance in plants and plant cells whereby enzymes involved in the NAD salvage synthesis pathway and/or the NAD de novo synthesis pathway are expressed in plants.
  • ROS reactive oxygen species
  • initial attempts to improve stress resistance in plants focused on prevention of the generation of the ROS or the removal thereof.
  • ROS scavenging enzymes such as catalases, peroxidases, superoxide dismutases etc. or even increasing the amount of ROS scavenging molecules such as ascorbic acid, glutathione etc.
  • Stress tolerance in plant cells and plants can also be achieved by reducing the activity or the level of the endogenous poly-ADP-ribose polymerases (ParP) or poly(ADP-ribose) glycohydrolases (ParG) as described in WO00/04173 and PCT/EP2004/003995, respectively. It is thought that in this way, fatal NAD and ATP depletion in plant cells subject to stress conditions, resulting in traumatic cell death, can be avoided or sufficiently postponed for the stressed cells to survive and acclimate to the stress conditions.
  • ParP poly-ADP-ribose polymerases
  • ParG poly(ADP-ribose) glycohydrolases
  • NAD synthetase from yeast has been well characterized and is the last enzyme in both the NAD de novo synthesis pathway and the NAD salvage pathway (see FIG. 1 ).
  • quinolate is the precursor for NAD synthesis and is generated as a product of tryptophan degradation.
  • nicotinamide (which is a degradation product of NAD, generated through the action of various enzymes such as PARP, NAD-dependent deacetylases or other NAD glycohydrolases) is the precursor molecule.
  • nicotinamide is deamidated to nicotinic cid by a nicotinamidase.
  • the nicotinic acid is transferred to 5-phosphoribosyl-1-pyrophosphate by the enzyme nicotinate phosphoribosyl transferase to yield nicotinic acid mononucleotide.
  • This compound is shared between the de novo and the salvage pathway. Hence, further conversion of this compound by NAD+ pyrophosphorylase and NAD synthetase is achieved as in the de novo pathway.
  • the identified DNA sequences have the following Accession numbers: for nicotinamidase: At5g23220; At5g23230 and At3g16190; for nicotinate phosphoribosyltransferase: At4g36940, At2g23420, for nicotinic acid mononucleotide adenyltransferase: At5g55810 and for NAD synthetase: At1g55090 (all nucleotide sequences are incorporated herein by reference).
  • a method for obtaining a plant with increased stress resistance comprising introducing a chimeric gene into a cells of a plant to obtain transgenic cells whereby the chimeric gene comprises the following operably linked DNA fragments:
  • the invention relates to the chimeric genes as described herein, plant cells comprising these chimeric genes, and plants consisting essentially of plant cells comprising these chimeric genes, and seeds of such plants.
  • These plants and plant cells may be characterized in that they have a lower level of reactive oxygen species under stress conditions than a similar plant not comprising such a chimeric gene.
  • the invention relates to the use of the described chimeric genes to increase the stress resistance of a plant or to decrease the level of reactive oxygen species in a plant or a plant cell under stress conditions.
  • the invention further provides the use of a DNA sequence encoding a plant functional enzyme of the nicotinamide adenine dinucleotide salvage synthesis pathway selected from nicotinamidase, nicotinate phosphoribosyltransferase, nicotinic acid mononucleotide adenyl transferase or nicotinamide adenine dinucleotide synthetase, such as a a DNA sequence encoding a protein comprising an amino acid sequence selected from the aminoacid sequence of SEQ ID No.:2, SEQ ID No.:4, SEQ ID No.:6; SEQ ID No.:8, SEQ ID No.:10, SEQ ID No.:12; SEQ ID No.:14; SEQ ID No.:16, SEQ ID No.:18, SEQ ID No.:20, SEQ ID No.: 22, SEQ ID No.:24 or a protein having about 60% sequence identity and having the enzymatic activity of
  • FIG. 1 is a schematic representation of the NAD salvage pathway and the de novo NAD synthesis pathway as known in baker's yeast ( Saccharomyces cerevisea )
  • FIGS. 2 to 11 are schematic representations of the various T-DNA vectors comprising DNA regions encoding enzymes from the NAD salvage pathway or the NAD de novo synthesis pathway under control of plant-expressible control elements.
  • Abbreviations used are: RB: right T-DNA border; 3′35S: transcription termination and polyadenylation signal from CaMV 35S transcript; Cab22L:untranslated leader sequence of the Cab22L transcript; P35S2: CaMV 35S promoter; 3′g7: transcription termination and polyadenylation signal from Agrobacterium tumefaciens T-DNA gene 7; bar: phosphinotricin acetyltransferase coding region; pSSUAra promoter of the Rubisco small subunit transcript from Arabidopsis ; LB; left T-DNA border; Sm/Sp: Spectinomycin and streptomycin resistance gene; pVS1ori; origin of VS1 suitable for replication in Agrobacterium ; ColE
  • the current invention is based on the finding that DNA sequences encoding plant-functional enzymes from the NAD salvage pathway in yeasts could be used to obtain transgenic plants which were more resistant to stress, particularly abiotic stress, than plants not comprising these DNA sequences.
  • the transgenic plants also exhibited a significantly reduced level of reactive oxygen species (“ROS”) and maintained a high level of NADH, when put under stress conditions, compared to control plants
  • ROS reactive oxygen species
  • a method is provided to obtain a plant with increased stress resistance, whereby the method comprises the steps of
  • the stress resistant chimeric gene thereby comprises a plant-expressible promoter operably linked to a DNA region coding for a plant-functional enzyme of the nicotinamide adenine dinucleotide salvage synthesis pathway selected from nicotinamidase, nicotinate phosphoribosyltransferase, nicotinic acid mononucleotide adenyl transferase or nicotinamide adenine dinucleotide synthetase and a 3′ end region involved in transcription termination and polyadenylation.
  • a plant-expressible promoter operably linked to a DNA region coding for a plant-functional enzyme of the nicotinamide adenine dinucleotide salvage synthesis pathway selected from nicotinamidase, nicotinate phosphoribosyltransferase, nicotinic acid mononucleotide adenyl transferase or
  • a plant-functional enzyme of the nicotinamide adenine dinucleotide salvage synthesis pathway is an enzyme which when introduced into plants, linked to appropriate control elements such as plant expressible promoter and terminator region, can be transcribed and translated to yield a enzyme of the NAD salvage synthesis pathway functional in plant cells. Included are the enzymes (and encoding genes) from the NAD salvage synthesis, which are obtained from a plant source, but also the enzymes obtained from yeast ( Saccharomyces cereviseae ) or from other yeasts or fungi. It is thought that the latter proteins may be even more suitable for the methods according to the invention, since these are less likely to be subject to the enzymatic feedback regulation etc. to which similar plant-derived enzymes may be subject.
  • Enzymes involved in the NAD salvage synthesis pathway comprise the following
  • the coding regions encoding the different enzymes of the NAD salvage pathway comprise a nucleotide sequence encoding proteins with the amino acid sequences as set forth in SEQ ID Nos 2, 4, 6, 8 or 10, such as the nucleotide sequences of SEQ ID Nos 1, 3, 5, 7 or 9.
  • nucleotide sequences including insertions, deletions and substitutions thereof may be also be used to the same effect.
  • homologues to the mentioned nucleotide sequences from species different from Saccharomyces cerevisea can be used. These include but are not limited to nucleotide sequences from plants, and nucleotide sequences encoding proteins with the same amino acid sequences, as well as variants of such nucleotide sequences. Examples of the latter are nucleotide sequences encoding a protein with an amino acid sequence as set forth in SEQ ID Nos 12, 14, 16, 18, 20, 22 or 24 such as the nucleotide sequences of SEQ ID Nos 11, 13, 15, 17, 19, 21 or 23.
  • variants of the described nucleotide sequence will have a sequence identity which is preferably at least about 80%, or 85 or 90% or 95% with identified nucleotide sequences encoding enzymes from the NAD salvage pathway, such as the ones identified in the sequence listing.
  • these variants will encode functional proteins with the same enzymatic activity as the enzymes from the NAD salvage pathway.
  • sequence identity of two related nucleotide or amino acid sequences, expressed as a percentage, refers to the number of positions in the two optimally aligned sequences which have identical residues ( ⁇ 100) divided by the number of positions compared.
  • a gap i.e.
  • a position in an alignment where a residue is present in one sequence but not in the other is regarded as a position with non-identical residues.
  • the alignment of the two sequences is performed by the Needleman and Wunsch algorithm (Needleman and Wunsch 1970).
  • the computer-assisted sequence alignment above can be conveniently performed using standard software program such as GAP which is part of the Wisconsin Package Version 10.1 (Genetics Computer Group, Madision, Wis., USA) using the default scoring matrix with a gap creation penalty of 50 and a gap extension penalty of 3.
  • Nucleotide sequences homologous to the nucleotide sequences encoding an enzyme from the NAD salvage pathway in yeast, or encoding a homologous enzyme from an organism different than yeast may be identified by in silico analysis of genomic data, as described by Hunt et al. (vide supra).
  • Homologous nucleotide sequence may also be identified and isolated by hybridization under stringent conditions using as probes identified nucleotide sequences encoding enzymes from the NAD salvage pathway, such as the ones identified in the sequence listing.
  • Stringent hybridization conditions means that hybridization will generally occur if there is at least 95% and preferably at least 97% sequence identity between the probe and the target sequence. Examples of stringent hybridization conditions are overnight incubation in a solution comprising 50% formamide, 5 ⁇ SSC (150 mM NaCl, 15 mM trisodium citrate), 50 mM sodium phosphate (pH 7.6), 5 ⁇ Denhardt's solution, 10% dextran sulfate, and 20 ⁇ g/ml denatured, sheared carrier DNA such as salmon sperm DNA, followed by washing the hybridization support in 0.1 ⁇ SSC at approximately 65° C., preferably twice for about 10 minutes. Other hybridization and wash conditions are well known and are exemplified in Sambrook et al, Molecular Cloning: A Laboratory Manual, Second Edition, Cold Spring Harbor, N.Y. (1989), particularly chapter 11.
  • variant sequences may also be obtained by DNA amplification using oligonucleotides specific for genes encoding enzymes from the NAD salvage pathway as primers, such as but not limited to oligonucleotides comprising about 20 to about 50 consecutive nucleotides selected from the nucleotide sequences of SEQ ID Nos 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23 or their complement.
  • the methods of the invention can be used to obtain plants tolerant to different kinds of stress-inducing conditions, particularly abiotic stress conditions including submergence, high light conditions, high UV radiation levels, increased hydrogen peroxide levels, drought conditions, high or low temperatures, increased salinity conditions.
  • the methods of the invention can also be used to reduce the level of ROS in the cells of plants growing under adverse conditions, particularly abiotic stress conditions including submergence, high light conditions, high UV radiation levels, increased hydrogen peroxide levels, drought conditions, high or low temperatures, increased salinity conditions etc.
  • the level of ROS or the level of NADH can be determined using the methods known in the art, including those described in Example 3.
  • plants may be obtained wherein the level of ROS is equal to or lower than in control plants under non-stressed conditions, such as but not limited to low light.
  • the level of ROS may range from 50% to 100% of the level of control plants under low light conditions, more particularly from about 60% to about 85%.
  • the level of the ROS in these plants under stress conditions is about 50% to 80% of the level of ROS in control plants under stress conditions, corresponding to about 60 to 80% of the level of ROS in control plants under non-stressed conditions.
  • the NADH level in these plants is equal to or higher than in control plants under non-stressed conditions, such as but not limited to low light.
  • the level of NADH may range from 100% to 160% of the level of NADH in control plants under low light conditions, more particularly from about 120% to about 140%.
  • the level of NADH in these plants under stress conditions is about 200 to 300% of the level of NADH in control plants under stress conditions, corresponding to about 100 to 160% of the level of ROS in control plants under non-stressed conditions.
  • transgenic plants are not deemed critical for the current invention and any transformation method and regeneration suitable for a particular plant species can be used.
  • Such methods are well known in the art and include Agrobacterium -mediated transformation, particle gun delivery, microinjection, electroporation of intact cells, polyethyleneglycol-mediated protoplast transformation, electroporation of protoplasts, liposome-mediated transformation, silicon-whiskers mediated transformation etc.
  • the transformed cells obtained in this way may then be regenerated into mature fertile plants.
  • the obtained transformed plant can be used in a conventional breeding scheme to produce more transformed plants with the same characteristics or to introduce the chimeric gene according to the invention in other varieties of the same or related plant species, or in hybrid plants.
  • Seeds obtained from the transformed plants contain the chimeric genes of the invention as a stable genomic insert and are also encompassed by the invention.
  • plant cells and plants which comprise at least two stress resistant chimeric genes each comprising a different coding region.
  • the transgenic plant cells and plant lines according to the invention may further comprise chimeric genes which will reduce the expression of endogenous PARP and/or PARG genes as described in WO 00/04173 and PCT/EP2004/003995. These further chimeric genes may be introduced e.g. by crossing the transgenic plant lines of the current invention with transgenic plants containing PARP and/or PARG gene expression reducing chimeric genes. Transgenic plant cells or plant lines may also be obtained by introducing or transforming the chimeric genes of the invention into transgenic plant cells comprising the PARP or PARG gene expression reducing chimeric genes or vice versa.
  • the promoter is a plant-expressible promoter.
  • plant-expressible promoter means a DNA sequence which is capable of controlling (initiating) transcription in a plant cell. This includes any promoter of plant origin, but also any promoter of non-plant origin which is capable of directing transcription in a plant cell, i.e., certain promoters of viral or bacterial origin such as the CaMV35S (Harpster et al., 1988 Mol. Gen.
  • tissue-specific or organ-specific promoters including but not limited to seed-specific promoters (e.g., WO89/03887), organ-primordia specific promoters (An et al., 1996 , The Plant Cell 8, 15-30), stem-specific promoters (Keller et al., 1988 , EMBO J.
  • leaf specific promoters Hudspeth et al., 1989 , Plant Mol Biol 12, 579-589
  • mesophyl-specific promoters such as the light-inducible Rubisco promoters
  • root-specific promoters Keller et al., 1989 , Genes Devel. 3, 1639-1646
  • tuber-specific promoters Keil et al., 1989 , EMBO J.
  • vascular tissue specific promoters Pieris et al., 1989 , Gene 84, 359-369
  • stamen-selective promoters WO 89/10396, WO 92/13956
  • dehiscence zone specific promoters WO 97/13865
  • the chimeric genes of the inventions may also be equipped with a nuclear localization signal (“NLS”) functional in plants, operably linked to the DNA region encoding an enzyme of the NAD salvage pathway such as the SV40 NLS.
  • NLS nuclear localization signal
  • variant plants can be obtained by subjecting a population of plants to mutagenesis, such as, but not limited to EMS mutagenesis, followed by a screening for an increased activity of any one of the NAD salvage pathway enzymes, or a combination thereof.
  • mutagenesis such as, but not limited to EMS mutagenesis
  • the methods and means described herein are believed to be suitable for all plant cells and plants, both dicotyledonous and monocotyledonous plant cells and plants including but not limited to cotton, Brassica vegetables, oilseed rape, wheat, corn or maize, barley, sunflowers, rice, oats, sugarcane, soybean, vegetables (including chicory, lettuce, tomato), tobacco, potato, sugarbeet, papaya, pineapple, mango, Arabidopsis thaliana , but also plants used in horticulture, floriculture or forestry.
  • nucleic acid or protein comprising a sequence of nucleotides or amino acids
  • a chimeric gene comprising a DNA region which is functionally or structurally defined, may comprise additional DNA regions etc.
  • a chimeric gene was constructed using conventional techniques comprising the following DNA fragments in order:
  • T-DNA vector pTVE467 is schematically represented in FIG. 2 .
  • T-DNA vector pTVE467 comprises the following molecule features:
  • (C) indicates complementary strand.
  • chimeric gene as present in pTVE467 was constructed, wherein the nicotinamidase was equipped with a conventional nuclear localization signal.
  • the chimeric gene thus comprises the following operably linked DNA fragments:
  • T-DNA vector pTVE468 is schematically represented in FIG. 3 .
  • T-DNA vector pTVE468 comprises the following molecule features:
  • (C) indicates complementary strand.
  • a chimeric gene was constructed using conventional techniques comprising the following DNA fragments in order:
  • T-DNA vector pTVE469 is schematically represented in FIG. 4 .
  • T-DNA vector pTVE469 comprises the following molecule features:
  • (C) indicates complementary strand.
  • chimeric gene as present in pTVE469 was constructed, wherein the nicotinate phosphoribosyltransferase from Saccharomyces cereviseae was equipped with a conventional nuclear localization signal
  • the chimeric gene thus comprises the following operably linked DNA fragments:
  • T-DNA vector pTVE470 is schematically represented in FIG. 5 .
  • T-DNA vector pTVE470 comprises the following molecule features:
  • (C) indicates complementary strand.
  • a chimeric gene was constructed using conventional techniques comprising the following DNA fragments in order:
  • T-DNA vector pTVE496 is schematically represented in FIG. 6 .
  • T-DNA vector pTVE496 comprises the following molecule features:
  • (C) indicates complementary strand.
  • chimeric gene as present in pTVE496 was constructed, wherein the nicotinic acid mononucleotide adenyl transferase 1 from Saccharomyces cereviseae was equipped with a conventional nuclear localization signal
  • the chimeric gene thus comprises the following operably linked DNA fragments:
  • T-DNA vector pTVE497 is schematically represented in FIG. 7 .
  • T-DNA vector pTVE497 comprises the following molecule features:
  • (C) indicates complementary strand.
  • a chimeric gene was constructed using conventional techniques comprising the following DNA fragments in order:
  • T-DNA vector pTVE500 is schematically represented in FIG. 8 .
  • T-DNA vector pTVE500 comprises the following molecule features:
  • (C) indicates complementary strand.
  • chimeric gene as present in pTVE500 was constructed, wherein the nicotinic acid mononucleotide adenyl transferase 2 from Saccharomyces cereviseae was equipped with a conventional nuclear localization signal
  • the chimeric gene thus comprises the following operably linked DNA fragments:
  • T-DNA vector pTVE501 is schematically represented in FIG. 9 .
  • T-DNA vector pTVE501 comprises the following molecule features:
  • (C) indicates complementary strand.
  • a chimeric gene was constructed using conventional techniques comprising the following DNA fragments in order:
  • T-DNA vector pTVE502 is schematically represented in FIG. 10 .
  • T-DNA vector pTVE502 comprises the following molecule features:
  • (C) indicates complementary strand.
  • a similar chimeric gene as present in pTVE502 was constructed, wherein the NAD synthase from Saccharomyces cereviseae was equipped with a conventional nuclear localization signal
  • the chimeric gene thus comprises the following operably linked DNA fragments:
  • T-DNA vector pTVE503 is schematically represented in FIG. 11 .
  • T-DNA vector pTVE503 comprises the following molecule features:
  • (C) indicates complementary strand.
  • the T-DNA vectors were introduced into Agrobacterium strains comprising a helper Ti-plasmid using conventional methods.
  • the chimeric genes were introduced into Arabidopsis plants by Agrobacterium mediated transformation as described in the art.
  • T1-generation transgenic Arabidopsis lines (T1-generation) expressing the yeast genes of the NAD-salvage pathway, obtained as described in Example 1 were germinated and grown on medium containing 15 mg L ⁇ 1 phosphinotricin (PPT). Arabidopsis thaliana cv Col-0 was used as a control.
  • PPT phosphinotricin
  • Transgenic plants exhibited a higher NADH content under high light than control plants, and produced less reactive oxygen species under high light than control plants. No difference was observed between constructs wherein the encoded NAD salvage pathway enzyme was equipped with a nuclear localization signal or not.
  • Transgenic plant lines were also phenotypically scored for tolerance to high light stress conditions. To this end, plants were grown in vitro at low light conditions (30 ⁇ Einstein m ⁇ 2 sec ⁇ 1 ) for two weeks and transferred for 3 days to high light conditions (250 ⁇ Einstein m ⁇ 2 sec ⁇ 1 ; 16 hrs light ⁇ 8 hrs dark). After the high light treatment the plants were returned to low light conditions and grown for another three days before scoring the phenotype.
  • Sowing medium (medium 201):
  • Seeds are soaked in 70% ethanol for 2 min, then surface-sterilized for 15 min in a sodium hypochlorite solution (with about 6% active chlorine) containing 0.1% Tween20. Finally, the seeds are rinsed with 11 of sterile tap water.
  • Seeds are put in 250 ml erlenmeyer flasks containing 50 ml of sterile tap water (+250 mg/l triacillin). Shake for about 20 hours.
  • Seeds from which the radicle is protruded are put in Vitro Vent containers from Duchefa containing about 125 ml of sowing medium (10 seeds/vessel, not too many to reduce loss of seed by contamination). The seeds are germinated at ⁇ 24° C. and 10-30 ⁇ Einstein s ⁇ 1 m ⁇ 2 with a daylength of 16 h.
  • the chimeric vector pTVE467 (Example 1) was used for transformation of A. thaliana ecotype Columbia. Primary transformants were analyzed by Southern-DNA- and Northern-RNA-blot analysis. One transgenic line was identified to carry a single copy of the Pnc1-transgene construct and to have a high steady state level of transgenic full-length Pnc1-mRNA (20 pg/5 ⁇ g total RNA).
  • PI vitality performance index
  • control plants homozygous transgenic populations of plants comprising the chimeric Pnc1 gene as well as a heterozygous transgenic population, were subjected to ozone fumigations and scored for visible injury and various physiological responses compared to non-fumigated plants.
  • the assessment included measurement of non-modulated fluorescence, modulated fluorescence, chlorophyll measurement and fresh weight determination.
  • control non-transgenic population and the heterozygous transgenic population had a cumulative score of ⁇ 13
  • the two homozygous transgenic populations had a score of ⁇ 6 and ⁇ 2 respectively. It is therefore clear that the homozygous transgenic populations performed statistically significantly better than the control plants.

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