SU1124974A1 - Method of storing blood - Google Patents

Abstract

A method of storing blood in a liquid state, characterized in that, in order to increase its storage time, blood under pressure of 400-500 atm is cooled, respectively, to (-5) - (- 6) C.

Description

This invention relates to medicines and can be used for blood storage. A known method of storing blood in a liquid state at 4 ° C with the preservative COLIP-78 ij. However, the known method does not provide long-term storage of blood while preserving the functional activity of its components. The aim of the invention is to increase the shelf life of blood. This goal is achieved in that according to the method of storing shelter. in the liquid state, the latter, under a pressure of 400-500 atm, is cooled, respectively, to (-5) - (- 6) C. Example. The container is pre-sterilized and filled with blood. Two types of containers are used: glass and rubber (silicone). The container is filled with distilled water and boiled for 30-45 minutes closed in a sterilizer bar for syringes. The sterilizer with the container is then transferred to the box. There is blood in the box, brought from the blood transfusion station. Blood stabilized with heparin at the rate of 5 units. 1 ml of blood taken in vials with a capacity of 50 ml. In a sterile box, a folded bottle of blood is opened, and blood is poured into a container previously removed from the sterilizer and rinsed with saline. This is done in order to avoid hemolysis with water, in which the container has been boiled. The blood-filled container is placed in a high pressure chamber, which is a cylinder with screw caps and electrical leads for measuring pressure and temperature. The cavity of the chamber fills glycerin, which is contactless, transfers the pressure to the sample, since the container with blood represents a cylinder with traffic jams playing the role of a piston. The chamber is pressurized with a hand pump. The next step, cooling the chamber, is carried out in a cryostat to a predetermined temperature. A blood sample is stored for a certain time; {5,10,20,30 days). Then the reverse process begins: the chamber is removed from the cryostat and after 15–20 min, when its temperature reaches room temperature, they begin to relieve pressure to atmospheric. Such a sequence of operations is necessary to prevent blood from freezing. The temperature in the container rises slowly over several minutes. In the case of rapid depressurization, the blood for some time is at a negative temperature, which inevitably leads to hemolysis. After depressurization, the sample is removed from the biochemical analysis chamber. The percentage of destroyed erythrocytes, hemoglobin content, potassium output in plasma, potassium content in erythrocytes are determined, Pjo is a characteristic indicator used to estimate the position of oxygen-dissociation.: Curves reduced to stationary conditions (pH7.4), using the P5o formula P + 0.48 pH 7.4, where P is the actually observed partial pressure of oxygen at pH of the experiment. The morphology is examined in smears under a microscope (the number of normocytes, spike cells, spherocytes is determined). In addition to examining the red row, white blood is being studied - the phagocytic activity of leukocytes by the bioluminescent test. All of these readings are compared with those previously tested on the control heparinized blood on the day of collection. Table 1 gives a comparative assessment of blood storage according to the proposed and known methods. A pressure of 400 atm and a temperature of (5 + 0.1) C are created in the chamber. Blood samples are stored under these conditions for 5-3 jO day (Table 1). After 5 days, the viability of blood cells is examined and the following results are obtained: destruction of red blood cells is less than 1% and is -O, 5 ± 0, 5%. No spherocytes and spinous cells were found, and in a known number their number during this time increases to 80%. The potassium content in the plasma according to the proposed method is 20.5 T 1.2 mg%, and according to the known content of potassium in the plasma increases to 33 days, 5 mg%, the content of potassium in the erythrocytes according to the proposed method by this time is 289 +3.0 mg%, which is higher than in the well-known (257 + 0.3 mg%). after 7 days of storage is 19.3 ± 1.29 mm Hg, and by a known method - M5.2 + 0.95 mm Hg. The phagocytic activity of leukocytes for 4 days of storage is almost unchanged (90-95%), and in the known is 0%. After the next 10, 20 and 30 days, almost all parameters characterizing the functional state of the blood (Table 2) are better than in the well-known. Table 2 shows the results of experiments on the storage of blood erythrocytes stabilized with heparin (the percentage destruction of erythrocytes). The advantages of the proposed method in comparison with the known one are that, in the well-known after 12 hours, leukocytes are practically

1 11249744

all are active, and in the solution, some of the proposed activity is toxic.

remains high even after 22 days

storage; The proposed method is effective. The proposed method also allows for more efficient preservation of blood.

properties of red blood; storage while maintaining its functional

blood does not require any preservative activity /

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Table 2

Claims (1)

METHOD FOR BLOOD STORAGE in a liquid state, characterized in that, in order to increase its shelf life, blood under pressure of 400-500 atm is cooled respectively to (-5) - (- 6) ° C.