RU2815970C1 - Method for improving qualitative and quantitative results of industrial incubation of chicken eggs with transovarial introduction of inositol - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: invention relates to agriculture, in particular to poultry farming. Method for industrial incubation of chicken eggs with transovarial introduction of inositol involves application of 0.01% aqueous solution of inositol from a sprayer onto the surface of the disinfected shell of incubation eggs, and when switching to output, applying 1% inositol solution. Aqueous solution of inositol is obtained by mixing inositol in distilled water at temperature of 18–22 °C.

EFFECT: proposed method for industrial incubation of chicken eggs provides reduction of abnormal intensity of lipid peroxidation processes, increased embryonic viability, hatchability, improved indices of young stock nervous system.

1 cl, 4 tbl, 4 ex

Description

This invention relates to the field of agriculture, in particular to poultry farming (pre-hatchery treatment of eggs with an aqueous solution of inositol).

Poultry farming is one of the key, actively developing branches of agriculture. In turn, incubation of poultry eggs is the basis of industrial poultry farming, on which the quality and quantity of young animals produced largely depends.

The physiological and biochemical formation of the bird’s body in ontogenesis occurs with certain characteristics. Unfortunately, industrial incubation cannot yet take into account all these features, and at the same time does not fully satisfy the requirements of an actively developing embryo. In this regard, this process is a multifaceted stressor, which causes excessive synthesis of free radicals and cytotoxic lipid peroxidation products. Their effect on the embryo during the incubation period has a detrimental effect on cell metabolism, which does not allow the body to fully and timely form organs and tissues and ensure homeostasis [8]. This reduces their functionality and the implementation of important productive qualities in further ontogenesis, determining a decrease in the economic efficiency of production.

To prevent and neutralize the consequences of exposure to strong and moderate stressors, substances of various chemical origins and effects are used. Natural metabolites that do not have xenobiotic effects are considered to be the most promising [9].

Such biostimulants prevent the negative consequences of free radical and lipid peroxide abnormalities by implementing antioxidant properties. In turn, it has been proven that their effectiveness increases when they are combined with energy-stimulating ones [8].

There are also substances that have a sedative effect, normalizing the functioning of the nervous system, relieving nervousness and tension, which often accompany the development of various stresses, but their use is permissible only in the preproductive period of ontogenesis [6].

One of the promising metabolites that simultaneously combines all previously stated properties is inositol, as well as its phosphorylation products [3].

Thus, according to recent studies, phytic acid in reasonable concentrations is not only not a toxic substance for the body, but has both its own antioxidant properties and exhibits high affinity for iron, maintaining it in the trivalent state, preventing the formation of hydroxyl radicals and other reactive oxygen species . Along with this, the metabolite is a donor of phosphate groups for ADP, allowing it to be converted into ATP [2,3]. Thus, phytic acid and other products of inositol phosphorylation are important participants in the energy process - substrate phosphorylation [1, 3].

It should be noted that inositol triphosphate is a secondary messenger in the membrane-intracellular mechanism of action of many hormones [4].

In turn, inositol itself, as part of phosphatidylinositols, is a component of cell membranes, the quality of construction of which largely determines the functionality, first of all, of the myelin components of nerve cells [4].

In this invention, the work chosen as a prototype is: Korsakov K.V. "A method for stimulating the embryonic development of birds." Patent No. 2687045, priority dated 05/06/2019 [10]. In the best batch, the hatchability of eggs exceeded the control by 3.30%, and the hatchability of chickens by 3.12%.

Previously, we proved the effectiveness of a single treatment of hatching eggs [11].

Our studies have proven that double treatment of eggs from a spray bottle with aqueous solutions of inositol in a certain concentration range stimulates the optimization of lipid peroxidation processes, as a consequence of thermoregulation and the formation of the most important neuro-reflex reactions, causing an increase in embryonic viability and the quality of development of young animals.

The purpose of the invention is to develop an effective method for improving the qualitative and quantitative results of industrial chicken incubation with transovarial administration of inositol.

The technical result of the invention is that under the influence of a solution of a biologically active substance: inositol, due to the correction of oxidative stress reactions and activation of energy processes, the intensity and quality of embryo development increases, and the hatching of conditioned chickens significantly increases (in the best experimental group by 7.04%).

The method is carried out as follows:

A 0.01% aqueous solution of inositol is applied to the surface of the disinfected shell of hatching eggs 3-4 hours before incubation from a spray bottle and, when transferring to hatching, a 1% solution of inositol. Inositol is mixed in distilled water at a temperature of 18-22°C until completely dissolved.

Example 1. The experiment was carried out on 5 batches of eggs, one of which was control, the others were experimental, the latter were treated with aqueous solutions of inositol before incubation at a previously established optimal concentration - 0.01%, and when transferred to hatching - in concentrations from 0.001% to 1 %. Eggs of the “Shaver White” cross hens used for transovarial processing were obtained from one parent flock, sorted into 300 pieces in each batch according to the principle of analogues, taking into account the time of laying, storage time and weight. The eggs were stored for 5 days at a temperature of 12°C and a humidity of 75%. BAS solutions were applied to the surface of the shell by irrigation according to the above scheme. The results of biocontrol of incubation are presented below.

Example 1. Double treatment of eggs with inositol solutions had a positive effect on the entire period of embryogenesis in a wide range of concentrations, causing a decrease in most categories of incubation waste and an increase in the embryonic viability of individuals. The maximum results were obtained in the 1st experimental batch. In it, the hatching of chickens exceeded the control by 12.25% (Table 1).

Table 1. Indicators of biocontrol of incubation, % (n=30)

The consignment Incubation waste Hatching chicks ±Δ Infertile. Blood rings Frozen Assholes Weak Control 6.05±1.33 2.55 ±0.70 7.02±1.42 6.73±1.24 2.7±0.8 74.95±3.73 - Experienced 1
(before incubation - 0.01% solution and on the 19th day 1% inositol solution) 2.07±
1.08* 0.75±0.4** 4.53±1.8 3.75±1.1* 1.7±0.3 87.2±3.87*** +12.25 Experienced 2
(before incubation - 0.01% solution and on the 19th day 0.1% inositol solution) 2.25±0.8* 1.1±0.35 4.85±1.12 4.53±1.07 1.5±0.6 85.77±
2.55** +10.82 Experienced 3
(before incubation - 0.01% solution and on the 19th day 0.01% inositol solution) 2.47±0.50* 0.9 ±0.32* 5.15±
1.25 3.8±
1.05* 1.7±
0.65 85.98±
2.41** +11.03% Experienced 4
(before incubation - 0.01% solution and on the 19th day 0.001% inositol solution) 2.36±
0.78* 1.5 ±
0.53 4.7±
1.26 4.2±
1.72 2.05±
0.88 85.19±
2.20* +10.24%

Note: Hereinafter *- p<0.05; **- p<0.01; ***- p<0.001

Example 2. Positive results obtained in all groups indicate the high biological activity of the substance under study.

The stated was confirmed by the higher quality of the resulting young animals, which is largely due to more advanced thermoregulation processes, and was expressed in an increase in the body temperature of day-old young animals of the best experimental group by 0.6 degrees compared to the control (Table 2), which, according to M. Epimakhova. E. (2013) indicates a higher biological usefulness of individuals.

Table 2. Rectal temperature at 24 hours, (n=10)

Index Group Control Experienced 1 Temperature, °C 38.5±0.13 39.1±0.14

Example 3. The better development of day-old young animals from the experimental groups was reflected in an increase in the score on the “Pasgar” and “Optistart” scales. The maximum result was also obtained when using a 0.01% inositol solution before incubation and when transferring to a 1% solution. This is due, first of all, to a significant significant superiority relative to the control in terms of “behavioral reflex” and “neck tone”, which indicates a better development of the nervous system in individuals obtained from treated eggs (Table 3).

The reaction to tapping confirmed the statement about the better development of chickens in the experimental groups and proved that the nervous system of the young animals in them is indeed more fully formed compared to the control.

Table 3. Quality of day-old chickens according to the “Pasgar” and “Optistart” scales, score (n=10)

Indicators Control Experience 1 Behavior reflex 1.5±0.06 2.0±0.00*** Umbilical ring 1.5±0.04 1.7±0.04* Metatarsus and toes 2.0±0.00 2.0±0.00 Beak 1.3±0.03 1.7±0.05** Stomach 2.0±0.01 2.0±0.00* "Pasgar" criterion 8.3±0.10 9.4±0.12*** Muscle tone of the neck 1.6±0.02 2.0±0.01 Behavior reflex 1.5±0.05 2.0±0.00*** Umbilical ring 1.5±0.04 1.7±0.04* Beak 1.3±0.06 1.7±0.05** Stomach 2.0±0.00 2.0±0.00* "Optistart" criterion 7.9±0.13 9.4±0.07 **

Note: hereinafter *P≤0.05;**- p<0.01; ***- p<0.001 relative to control.

Example 4. It is obvious that the above positive biological effects were due to the implementation of the antioxidant properties of inositol.

Table 4. Indicators of lipid peroxidation and antioxidant defense of the body (n=5)

Group
Indicators AOA, % ODK,
unit of opt.pl./ml MDA, units of optical density/ml OR, unit of opt.pl./ml DC unit opt.pl./ml TC unit opt.pl./ml Control 41.0±1.65 3.1±0.5 5.1±0.7 0.5±0.21 1.95±0.30 1.12±0.2 Experienced 1 47.0±1.35 2.7±0.22* 1.45±0.34** 0.3±0.11* 1.60±0.25 1.00±0.2

Note: here *P≤0.05;**- p<0.01; ***- p<0.001 relative to control.

From Table 4 presented above it can be seen that in the experimental group the TDC was lower by 13% (p<0.05) than in the control with a tendency towards a decrease in DC and TC, which indicates the prevention of destructive phenomena, primarily in the structure: lipoproteins, proteins, enzymes and nucleic acids [2] . In turn, there was a significant decrease in malondialdehyde by 3.5 times (p<0.05) and Schiff bases by 1.7 times (p<0.05), respectively, which highly likely indicates the absence of conditions for initiation autoimmune processes [4].

Obviously, the decrease in the concentration of the cytotoxic lipid peroxidation products presented above was due to an increase in AOA by 6%.

Conclusion: transovarial use of inositol, due to the implementation of its antioxidant properties, which prevent the development of oxidative stress, and at the same time the appearance of destructive phenomena in the cell, made it possible to significantly increase the qualitative and quantitative results of incubation.

Literature

1. Graf E. and Eaton J. Antioxidant function of phytic acid. // W. Free Radical Biology & Medicine, - 1990. - No. 8. - P. 61-69.

2. Graf E., Empson K.L. and Eaton J.W. Phytic acid. A natural antioxidant. // J. of biological chemistry. – 1987.- No. 24. - P. 11647-11650.

3. Bennett M.S., Onnebo M.N., Azevedo C. and Saiardi A. Inositol pyrophosphates: metabolism and signaling.- // Cell. Mol. Life Sci. - 2006. - No. 63. - P. 552–564.

4. Berridge M.J. Inositol trisphosphate and diacylglycerol as second messengers. // Great Britain.- 1984.- No. 220. – P. 345-360.

5. Vladimirov Yu.A., Azizova O.A., Deev A.I. and others. Free radicals in living systems // Results of science and technology. Biophysics. 1992. T. 29. P. 3–250.

6. Galochkin V.A., Ostrenko K.S., Galochkina V.P., Fedorov L.M. The relationship between the nervous, immune, endocrine systems and nutritional factors in the regulation of resistance and productivity of animals. // Agricultural biology. - 2018. volume 53, no. 4. - pp. 673-686.

7. Kochish I.I. Transovarial use of a drug containing selenium and vitamin C to optimize temperature homeostasis in day-old chicks /I.I. Kocsis, T.O. Azarnova, D.L. Bogdanova, M.S. Naydensky // Problems of veterinary sanitation, hygiene and ecology.-2017.-No.2-P.117-120.

8. Azarnova T.O. Scientific and practical aspects of the prevention of oxidative stress as a way to optimize the conditions of incubation and acceleration of chicken embryos: abstract of thesis. dis. ...Dr.Biol. Sciences: 06.02.05, 03.01.04 / Azarnova Tatyana Olegovna.- M., 2013.- 49 p.

9. Gubsky Yu.I. Cell death: free radicals, necrosis, apoptosis: monograph - Vinnitsa: Nova Kniga, 2015. - 360 p.

10. Patent No. 2687045 of the Russian Federation, IPC A01K 67/00 Korsakov K.V. Method for stimulating embryonic development of poultry; patent holder Korsakov K.V./No. 2018134368 10/01/2018; application 01.10.2018; published 05/06/2019 Bulletin. No. 13.

11. Azarnova, T.O. Prospects for the use of the stress protector inositol in egg farming / T.O. Azarnova, E.S. Panina, M.S. Naydensky, I.S. Lugovaya, D.V. Anshakov // Poultry and poultry products. - No. 1. - M. - 2019. - P.44-46.

Claims (1)

A method for industrial incubation of chicken eggs with transovarial administration of inositol, characterized by the fact that a 0.01% aqueous solution of inositol is applied to the surface of the disinfected shell of hatching eggs 3-4 hours before incubation from a spray bottle, and when transferring to output, a 1% solution of inositol is applied by mixing inositol in distilled water at a temperature of 18-22°C.