RU2486752C1 - Method of stimulation of embryogenesis of egg production hens and prevention of physiological exposure to stress - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: present invention relates to poultry breeding, in particular, to a method of stimulation of embryogenesis egg production hens and prevention of physiological exposure to stress in the most critical periods of embryonic development. The method of stimulation of embryogenesis egg production hens and prevention of physiological exposure to stress in the most critical periods of embryonic development by reducing the peroxidation processes, optimisation of the metabolic rate, increase in intensity of hatching with high viability of the youngsters for a long period of post-embryonic development by the method of treatment the hatching eggs includes treatment of the hatching eggs. The treatment of the hatching eggs is carried out twice, namely 3-4 hours before placing in the incubator - on the shell surface an aqueous solution of the preparations is applied from the sprayer: colamine, succinic acid and serine at concentrations of 0.1%, 0.1%, 0.2 %, respectively, and on day 19 - 0.1% aqueous solution of colamine, and the preparations are preliminary dissolved in distilled water at 18-22°C.

EFFECT: use of the invention enables to stimulate embryogenesis of egg hens and to increase hatching of standard poults.

5 tbl, 4 ex

Description

This invention relates to the field of agriculture and, in particular, to poultry farming (aerosol application of a solution that includes colamine, amino acid serine and succinic acid before incubation and processing of incubated eggs on the nineteenth day).

Known treatment of hatching eggs with solutions of serine, colamine and succinic acid in order to stimulate the development of agricultural birds. Succinic acid and its salts have adaptogenic ability and have antihypoxic, anti-stress, antioxidant and neurotropic effects, normalize energy and plastic metabolism and the general physiological state of the body. Colamine inhibits the oxidation of fats, vitamin A and other compounds with unsaturated carbon bonds, takes an active part in redox processes in animals, improves phosphorus and protein metabolism and, therefore, is a growth stimulator. The combination of these drugs is not jointly registered. The use of serine to stimulate embryonic and postembryonic development has not been studied. Also, these natural metabolites have never been used to prevent stress in birds.

These methods, which we took as prototypes, consist in the fact that succinic acid was used in the processing of eggs of egg cross. Egg hatchability by 5-7.01%. With colamine solutions, the eggs of broiler chickens were treated once before incubation. Egg hatchability was 3.5–4.24% (p <0.05) higher than in the control group.

The purpose of the invention is the stimulation of embryogenesis of egg chickens, an increase in the output of conditioned chickens, the prevention of stress by aerosol treatment of eggs before laying for incubation with an aqueous solution of drugs: colomin, succinic acid and serine, and on the 19th day of incubation with a colamine solution.

The method is as follows.

An aqueous solution of drugs - colamine, succinic acid and serine is applied to the surface of the shell 3-4 hours before incubation from the spray gun: 0.1% 0.1%, 0.2%, respectively, and on the 19th day - 0, 1% aqueous solution of colamine. Preliminarily, the preparations are dissolved and mixed in distilled water at 18-22 ° C.

Example 1. Eggs of hens cross "Shaver 2000". Received from one parent herd, sorted by 306 pieces per batch, subject to similar mass, demolition time and shelf life. On the surface of the shell, a solution of the preparations was applied in the form of an aerosol according to the above scheme. As a control, eggs of the same batch were selected according to the principle of analogues, which were processed according to the technology adopted at the farm. The data obtained are presented in table 1. From the table it follows that the conclusion from the experimental batch exceeds the control by 9.38%, and the deduction by 9.26%. Such incubation wastes significantly decreased: “blood rings” 5.6 times, “frozen” 2.24 times, “suffocates” 2 times, “weak” 0.37%.

Example 2. The laying of eggs cross "Shaver 2000" for incubation of the experimental and control groups was made 04.04.11, at 12 o’clock. 07/26/2011 withdrawal began, lasting 23 hours, which is 15 hours ahead of the withdrawal rate. So the development of allantois in the experimental group on the 11th day is significantly ahead of control, as can be seen from table 2.

Example 3. Chickens hatched from processed eggs (example 1) were grown under the conditions of the same farm to 30 days of age in cell batteries. The data are presented in table 3. In the experimental group over the entire study period, the safety of young animals increased by 3%, respectively, compared with the control.

Table 3 The safety of chickens,% n = 100 Groups of chickens Control Experienced Age in days 1-10 case four 2 coxp. 96 ± 1.96 98 ± 1.4 10-20 case one 2 coxp. 99 ± 0.99 98 ± 1.44 20-30 case one one coxp. 98 ± 1.4 99 ± 0.99 In just 30 days case 6 5 coxp. 94 ± 2,37 95 ± 2.18

Example 4. In chickens hatched from processed eggs, at the age of 24 hours, a biochemical study of blood serum was established: the total protein content in the blood serum of the chickens of the experimental groups increased by 5.4%, α-amylase by 7.9%, and blood glucose 2.2%, an increase in lysozyme activity by 4.8%. The data are presented in table 4.

As can be seen from table 5, the drug has a powerful antioxidant effect in the body of chickens. The table shows that the intensity of peroxidation in the experimental group is significantly lower than in the control. Thus, the level of the secondary product of lipid peroxidation in the form of malondialdehyde (MDA) decreased by 11.8%. An interesting fact is that with a decrease in the intensity of peroxidation processes, the antioxidant enzymatic protection of the body of chickens of 24-day-old age has increased, which has resulted in an increase in SOD by 70%. All biochemical parameters were within the physiological norm.

Table 5 Indicators of lipid peroxidation (lipid peroxidation) and antioxidant defense system (AODS) Group peroxidase unit opt.pl./l * s SOD, act / mg of hemoglobin OSH, rel. u / ml MDA, μmol / L Control 24 ± 2,00 1.0 ± 0.15 0.4 ± 0.018 1.7 ± 0.181 Experience 29 ± 1.22 1.7 ± 0.13 0.3 ± 0.014 1.5 ± 0.13

Literature

1. Bryushinin N.V. The use of organic acids for the treatment of hatching eggs / N.V. Brushinin // Vseros. scientific method. conf. on pet hygiene, init. Danilova A.K .: Thesis. doc. - M., 2003 .-- 57-58 s.

2. Kondrasheva M.N. Preface / MN Kondrasheva // Amber acid in medicine, food industry, agriculture: Sat. scientific tr Pushkin Scientific Center of the Russian Academy of Sciences - Pushkino, 1997, p.5.

3. Lukicheva V.A. The effect of succinic acid and glycine on protein metabolism, hatching and hatchability in broilers of the Smena-2 cross / V.A. Lukikheva, S.Yu. Zaitsev, M.S. Naidensky // Materials of the XI Moscow Int. vet. Congress. - M., 2003 .-- 238 p.

4. Burlakov S.S. The influence of biologically safe methods of processing eggs on the embryonic development of chickens / S.S. Burlakov // Vseros. conf. young scientists and graduate students in poultry: Abstracts dokl. - Sergiev-Posad, 1999 .-- 11 p.

5. Kondrashova M.N. Regulation of succinic acid energy support and the functional state of the tissue / MN Kondrashova. - Pushchino, 1979.- 34 p.

6. Meister A. Biochemistry of amino acids / A. Meister. - M .: Publishing house of foreign countries. lit., 1961 .-- 235 p.

7. Patent RU 2387130. A method of stimulating embryonic and embryonic development of broilers.

Claims (1)

A method of stimulating embryogenesis of eggs in the productivity direction and prevention of physiological stress exposure in the main critical periods of embryo development by reducing peroxidation processes, optimizing the metabolic rate, increasing the output intensity with high viability of young animals for a long period of post-embryonic development by treatment of incubation eggs, characterized in that the processing hatching eggs are carried out twice, namely, 3-4 hours before laying in the incubator the shell shell is sprayed with an aqueous solution of preparations: colamine, succinic acid and serine in concentrations of 0.1%, 0.1%, 0.2%, respectively, and on day 19 - 0.1% aqueous solution of colamine, and the preparations pre-dissolved in distilled water at 18-22 ° C.