RU2368391C1 - Method of getting complex immuno stimulating substance for improvement of autarcesis of animal organism - Google Patents

Abstract

FIELD: veterinary science.

SUBSTANCE: method presupposes mixing in the water of amber acid with Dorogov's stimulator antiseptic of the second fraction, novocain; getting pH to the level of 6.9-7.0, sterilisation by autoclaving in the regimen of 1.0-1.1 atmospheres within 20 minutes. Cyancobalamin and glucose are additionally introduced into the substance at the following components ratio, wt %: "АСД-Ф"-№ 2 - 4.0, amber acid - 1.0, cyancobalamin - 0.015, glucose - 4.0, novocain - 0.25, distilled water - the rest.

EFFECT: method makes it possible to get a substance with complex immune-stimulating effect.

2 tbl, 1 ex

Description

The invention relates to veterinary medicine and relates to methods for producing immunostimulating drugs to increase the natural resistance of the animal organism.

Known immunostimulating drug "Vitstim" (RF Patent 2232501, 08.26.2002). Vitstim contains ascorbic acid - 3000 mg / 100 ml, cyancobalamin - 15 mg / 100 ml, sodium chloride - 500 mg / 100 ml and potassium chloride - 100 mg / 100 ml, sodium bicarbonate - 400 mg / 100 ml, antiseptic - Dorogov stimulator ASD-F-No.2 - 10000 mg / 100 ml.

An important distinguishing feature of this drug is the complex composition of water-soluble components. The disadvantage of this drug is increased reactogenicity, which may be due to the high concentration of ASD-F-No.2 (10%). As you know, ASD-F-No. 2 has a very high alkaline reaction, which requires its neutralization with acid. However, the use of ascorbic acid for this purpose cannot be considered the optimal solution because it reacts with an alkali and is rapidly destroyed, while it is incompatible with carbonates (Mozgov I.E. Pharmacology, 1979, p. 218-219 )

A known method of obtaining the drug "amber biostimulant" to increase the resistance of the animal organism (RF Patent No. 2303979, from 10.08.2007). The method consists in obtaining a drug having a pronounced immunostimulating effect, containing succinic acid in its composition - 1%; ASD-F-No. 2 - 4%; novocaine - 0.25%. However, an amber biostimulant is not effective enough to normalize digestion in diarrhea against the background of vitamin B ₁₂ deficiency.

The objective of the invention is to obtain an injectable form of an immunostimulating drug with increased biological activity compared to the above counterparts. The problem is achieved by the inclusion of the following components in the complex immunostimulating drug, wt.%:

ASD-F-No.2 - 4.0%

Cyanocobalamin - 0.015%

Succinic acid - 1.0%

Glucose - 4.0%

Novocaine - 0.25%

Distilled water is the rest.

This ratio as a result of the studies was identified as the most optimal and allows you to strengthen immunostimulating activity. Given the greater similarity of the test drug with the analogue, we propose to call the new drug "amber biostimulant - plus." The resulting composition was sterilized by autoclaving in the 1.0-1.1 atm mode for 20 minutes.

Unlike analogues, the preparation obtained by the claimed method contains cyanobalamin and glucose in its composition. In the body, cyancobalamin is biotransformed and is part of the enzymes involved in the biosynthesis of structural and functional proteins, is involved in the metabolism of amino acids, carbohydrates, and is a regulator of the formation of erythropoiesis. Cyanocobalamin is used for poor development, growth retardation of young animals, chronic gastrointestinal diseases, anemia, weakening of the detoxifying ability of the liver, and _12- vitamin deficiencies. The inclusion of glucose is due to the need to provide the body of a sick animal experiencing an energy crisis with fast, readily available energy material. This is especially important for diarrhea, when the animal’s body is energetically weakened. The method of preparation of the drug is presented by example.

Example. To prepare a complex immunostimulating drug, 900 ml of distilled water was used in which 10 g of succinic acid, 150 mg of cyancobalamin, 40 g of glucose, 2.5 g of novocaine were successively dissolved at a temperature of 50-60 ° C. After the solids were completely dissolved, 40 ml of ASD-F-No.2 was added. The addition of water brought the volume of the drug to 1000 ml. The pH was adjusted to 6.9-7.0 by fractional addition of 10% sodium hydroxide. The resulting preparation was packaged in 50.0 ml vials and autoclaved in a 1.0-1.1 atm mode for 20 minutes. The drug was tested in comparison with an amber biostimulator and Vitstim on hypotrophic piglets of two weeks of age.

The test drugs were used twice with an interval of 3 days. The preparations were administered intramuscularly in a volume of 1.0 ml for the first time and 1.2 ml for the second time. Control studies of hematological blood parameters and live weight were performed at 15 and 30 days old. The data of the studies are presented in table 1. Interpretation of these indicators indicates that in piglets of the second group, the hemoglobin content in the blood exceeded the control values of peers from groups 1 and 3 by 9.1% -21.4% respectively at the age of 15 days and by 29.7% -37.6% at the age of one month.

A similar picture can be seen in terms of the content of red blood cells, protein, globulin fractions. This indicates an increase in the state of natural resistance.

In the second series of experiments, the effectiveness of the use of the drug "amber biostimulator - plus" in comparison with the amber biostimulator was carried out on pigs of one month of age in anticipation of weaning them from their mothers.

Table 1 Benchmarks in piglets after drug administration Baseline preparations Vitstim Amber Biostimulator + Amber Biostimulator Age, days fifteen fifteen fifteen Live weight kg 2.45 ± 1.01 2,50 ± 1,21 2.45 ± 1.11 Red blood cells, mln / ml 6.10 ± 0.74 6.30 ± 0.69 6.30 ± 0.51 Hemoglobin, g /% 9.80 ± 0.53 11.90 ± 0.37 10.70 ± 0.49 White blood cells, thousand / ml 5.70 ± 0.34 5.90 ± 0.25 5.70 ± 0.31 Total protein, g /% 6.70 ± 0.24 8.10 ± 0.45 7.80 ± 0.29 α - globulins fourteen fifteen fourteen β - globulins 16 17 16 γ - globulins 17 19 eighteen Performance after 15 days Live weight kg 5.90 ± 0.53 6.30 ± 0.41 6.00 ± 0.35 Red blood cells, mln / ml 8.70 ± 0.54 9.30 ± 0.35 9.10 ± 0.55 Hemoglobin, g /% 10.10 ± 0.31 13.90 ± 0.25 13.10 ± 0.29 White blood cells, thousand / ml 6.90 ± 0.42 7.70 ± 0.27 7.50 ± 0.33 Total protein, g /% 6.50 ± 0.31 7.50 ± 0.33 7.10 ± 0.51 α - globulins fifteen twenty 17 β - globulins 17 23 19 γ - globulins 19 25 21

Piglets of the first experimental group (n = 25) were injected intramuscularly twice with an interval of 10 days with an “amber biostimulant plus” in a volume of 2.5 and 3.0 ml. Piglets of the second experimental group (n = 25) were given “amber biostimulant” on the same days and in the same volume. Piglets of the third group (n = 25) were control.

Clinical observations indicated a virtually complete absence of gastrointestinal diseases with signs of diarrhea and enterotoxic phenomena in piglets of the first experimental group. In the second experimental group, signs of mild diarrhea were observed in 6 individuals (24%) in the absence of enterotoxic phenomena. In the third group, signs of diarrhea were observed in 11 animals (44%), and in 6 of them with enterotoxemia.

At 45 days of age, 5 days after weaning the animals from their mothers, a hematological blood test was performed, the results of which are presented in table 2.

table 2 The effect of the amber biostimulator and the amber biostimulator plus on the hematological parameters of experimental piglets Indicators preparations Amber Biostimulator Amber Biostimulant Plus Red blood cells, mln / ml 5.47 ± 0.23 5.55 ± 0.11 Hemoglobin, g /% 11.80 ± 0.31 12.30 ± 0.27 White blood cells, thousand / ml 11.36 ± 0.37 12.76 ± 0.47 FASK,% 35.40 ± 0.75 38.60 ± 0.46 LASK,% 38.10 ± 0.22 40.10 ± 0.24 Wed day gain, g 320,0 350,0 FASK - phagocytic activity of blood serum LASK - lysocytic activity of blood serum

Analysis of indicators allows us to note a significant increase (p <0.05) in hemoglobin and leukocytes in the blood of piglets when using the drug "amber biostimulant - plus." At the same time, there was a significant increase in FASK - by 3.2% and LASK - within 2.0% and an increase in the average daily gain in live weight of piglets.

Thus, the drug obtained by the claimed method, allowed a more pronounced improvement in the state of natural resistance, which favorably affected the clinical condition of experimental animals, their resistance to gastrointestinal diseases and growth activity.

Clinical studies have confirmed the correct selection of the components of the drug and their quantitative content, which together contributed to the solution of the problem.

The claimed method provides a positive effect - obtaining an immunostimulating drug with increased biological activity, the injection of which enhances the natural resistance of the animal organism.

Claims (1)

A method of obtaining a complex immunostimulating drug to increase the resistance of an animal organism, including mixing succinic acid with an antiseptic Dorogov stimulator in water of the second fraction, novocaine, adjusting the pH to 6.9-7.0, sterilization by autoclaving in the regime of 1.0-1.1 atm for 20 minutes, characterized in that it is additionally administered cyancobalamin and glucose in the following ratio of components, wt.%:
ASD-F-№2 4.0 succinic acid 1,0 Cyanobalamin 0.015 Glucose 4.0 Novocaine 0.25 Distilled water Rest