RU2302873C2 - Preparation for prophylaxis and treatment of anemia and correction of metabolism disturbance in agricultural animals - Google Patents

Abstract

FIELD: veterinary science.

SUBSTANCE: invention proposes a preparation that comprises starch, copper sulfate, manganese sulfate and pancreatic casein hydrolyzate. As an iron-containing compound the preparation comprises ferric chloride in the following ratio of components, wt.-%: ferric chloride, 3.0-3.5; starch, 16.0-18.0; copper sulfate, 0.005-0.006; manganese sulfate, 0.005-0.006; pancreatic casein hydrolyzate, 1.2-1.5, and water, the balance. The preparation shows prophylactic and curative effects in anemia in piglets and promotes to correction of metabolism disorders in agricultural animals.

EFFECT: valuable properties of preparation.

7 tbl, 8 ex

Description

The invention relates to agriculture, specifically to veterinary medicine, and is intended for the prevention and treatment of anemia, the correction of metabolic disorders in farm animals.

A number of drugs are known for the prevention and treatment of iron deficiency anemia in piglets. In particular, imposil (England), trifervetrin (France), ferrodex (Poland), ursoferran (Germany), ferroglukin-75 (Russia) and others. All of them are a complex compound of low molecular weight fractions of dextran with ferric iron (Veterinary preparations. M. : Kolos, 1981, 448 pp .; Veterinary preparations, Moscow: Agropromizdat, 1985, 309 pp .; I.F. Klenova, N.A. Yaremenko. Veterinary preparations in Russia. Moscow: Selkhozizdat, 2001, 546 pp. )

However, the described agents are not effective enough due to the fact that they contain only iron cations and cannot compensate for the deficiency in the body of other components necessary for blood formation and metabolic correction, and they are also difficult to absorb from the injection site.

Closest to the invention in technical essence is a drug against anemia - ferroglukin-75 (TU 64-3-89-85), which is a complex of ferric iron with a low molecular weight fraction of destran.

However, this drug is intended only for the prevention of iron deficiency anemia in piglets and does not provide the body with the need for other substances necessary for hematopoiesis, in addition, it is absorbed from the injection site, which reduces the quality of meat.

The objective of the invention is to obtain a more effective integrated drug for the prevention and treatment of anemia in farm animals.

To achieve this technical result, starch, copper sulfate and manganese sulfate, pancreatic casein hydrolyzate (TU 42-14-1-74) are additionally introduced into the preparation for the prophylaxis and treatment of anemia, and iron trichloride is used as the iron-containing compound the following ratio of components, wt.%:

trichloride iron 3.0-3.5 starch 16.0-18.0 copper sulfate 0.005-0.006 manganese sulfate 0.005-0.006 casein pancreatic hydrolyzate 1.2-1.5 water rest

The introduction of starch, copper sulfate, manganese sulfate, pancreatic casein hydrolyzate, and the use of iron trichloride as an iron-containing compound with the above ratio of components contributes to good absorption from the injection site, increases the effectiveness of the prevention and treatment of anemia in farm animals due to normalization of metabolism.

The drug for the prevention and treatment of anemia, the correction of metabolic disorders in farm animals is obtained in an enamel or glass container by boiling a suspension of polysaccharide (starch) in a solution of ferric chloride at 100 ° C for 30 minutes, followed by neutralization with a solution of sodium hydroxide, then additionally add weighed portions of pancreatic casein hydrolyzate, copper and manganese salts.

Examples of the manufacture of the drug.

Example 1. Take 160 g of starch, suspended in 1 liter of distilled water. The suspension is brought to boiling, 30 g / l of iron trichloride (GOST 4147-74) is added and boiling is continued for 30 minutes. Then the solution is cooled and neutralized 5 N. sodium hydroxide solution (GOST 4328-77) to pH 6, 5, after neutralization, copper sulfate (GOST 4165-78) is added to it - 50 mg / l, manganese sulfate - 50 mg / l (GOST 435-77) and casein hydrolyzate pancreatic - 15 g. This composition is sterilized by boiling for 30 minutes or by autoclaving at 1 atm for 20 minutes. Then poured into bottles.

Example 2. Take 180 g of starch and suspend in 1 l of distilled water, bring to a boil, add 35 g / l of iron trichloride and boil for 30 minutes The solution is cooled and neutralized 5 N. sodium hydroxide solution to a pH of 6.5. Then, weighed portions of copper sulfate — 60 mg / L, manganese sulfate — 60 mg / L, and 12 g — of pancreatic casein hydrolyzate. Sterilization is the same as in example No. 1.

The test results of the main physicochemical properties of different drugs are given in table 1. Both series of the drug were sterile, i.e. when inoculated into culture media, the growth of microorganisms was not observed. They had a dark brown color, pH 6.4-6.6, did not adversely affect white mice with subcutaneous and intravenous doses of 0.2-0.3 ml. The preparations did not have an irritant effect when administered intramuscularly to rabbits and piglets at a dose of 1.0-2.0 cm ³ per 1 kg of live weight.

The main physicochemical properties of the drug and biological activity remained unchanged for 2 years after manufacture (observation period).

Table 1
Indicators of the main properties of the drug No. p / p Indicators results Example 1 Example 2 Example 3 Example 4 one. Viscosity Slightly viscous liquid 2. Color dark brown dark brown brown dark brown 3. Sterility Sterile four. Harmlessness non-toxic does not cause death of animals, does not have local irritant effect Local injection at the injection site. reaction 5. Copper sulfate (wt.%) 0.005 0.006 0.005 0.005 6. Sulfurous manganese (wt.%) 0.005 0.006 0.005 0.005 7. drug pH 6.5 6.4 6.6 6.6 8. Hematopoiesis stimulation pronounced pronounced mild mild

To establish the optimal amount of ferric chloride conducted additional studies, which are shown in examples No. 3 and No. 4.

Example 3. They took iron chloride in an amount of 25 g / l, the remaining ingredients are the same as in example No. 1.

Example 4. They took iron chloride in an amount of 40 g / l, the remaining amount is the same as example No. 1. At the same time, it takes 15-20 ml more caustic soda to neutralize the solution. Therefore, an inflammatory reaction is noted at the injection site, which is undesirable.

The main properties of the proposed drug, summarized in table 1, indicate that the variation in the quantitative content of the components above the specified limits does not significantly affect the viscosity, sterility, color and biological activity of the drug. However, the drug obtained, as in example 3, the stimulation of hematopoiesis was relatively less compared with examples 1 and 2.

This was especially evident when these drugs were injected after artificial bloodletting. Therefore, the most optimal is the introduction of iron trifluoride in the amount of 30-35 g / l, copper sulfate and manganese - 50-60 mg / l. With a decrease in the amount of ferric chloride to 25 g / l, the stimulation of hematopoiesis decreased, and with an increase in its content to 40 g / l or more, a local irritant effect was observed.

The results of experiments to study the effect of the proposed drug on the content in the blood of animals of red blood cells and hemoglobin are given in table 2. As the results of the studies showed, a few days after injection of the drug in animals, there was a slight increase in the number of red blood cells and hemoglobin in the blood, but not beyond the physiological norm. This indicates a positive effect on blood formation. Especially stimulating effect on hematopoiesis was manifested in rabbits after artificial bloodletting. In particular, three rabbits were given daily 10 ml bloodletting over 4 days. Then, two of them were injected intramuscularly in a dose of 2 ml of the proposed drug, and one served as a control, which was injected with physiological solution of sodium chloride. After 5 days in the first two rabbits, the hemoglobin content was 10.2 g%, erythrocytes - 5.3 × 10 ¹² / L, and in the control - 8.5 g% and 4.7 × 10 ¹² / L, respectively.

table 2
Hematological parameters in animals before and after injection of the proposed drug Animal species Dose of the drug (ml) The number of red blood cells × 10 ¹² / l The amount of hemoglobin g% before injection b / w 5 days. before injection b / w 5 days. Guinea pigs 1,0 4.7 ± 0.5 5.6 ± 0.3 12.5 ± 0.4 14.7 ± 0.2 Rabbits 2.0 5.0 ± 0.4 6.0 ± 0.3 10.9 ± 0.2 11.8 ± 0.3 Piglets 1,5 6.2 ± 0.5 7.0 ± 0.4 9.1 ± 0.3 10.7 ± 0.2 Calves 10.0 6.5 ± 0.6 7.0 ± 0.5 11.1 ± 0.3 11.8 ± 0.4

Table 3 shows the results of one of the experiments to determine the proposed drug. In the leukoformula in piglets before and after injection of the drug, there was no significant difference (P <0.05), which indicates the absence of a negative effect of this drug on the animal organism.

Table 3
Leukoformula blood piglets before and after injection of the proposed drug Blood cells before introduction after 5 days Basophils one 0 Eosinphils 5 four Young neutrophils 0 one Stab neutrophils 3 four Segmented 42 43 Lymphocytes 46 44 Monocytes 3 four TOTAL one hundred one hundred

The results of a comparative study of the effectiveness of the proposed drug on piglets are given in table 4. Before the injection of iron-containing preparations, the content of hemoglobin and erythrocytes in all four groups of piglets was at the same level and amounted to 7.6-7.8 g% and 4.2-4.3 × 10 ¹² / l, respectively, which is lower than physiological indicators. This indicates the beginning of the development of anemia in them.

Table 4
Comparative characteristics of the effect of iron-containing preparations on the hematological parameters of piglets contained in the pig complex (n = 5) Age of piglets (days) Hemoglobin g% Red blood cells × 10 ¹² / L Group 1 - the introduction of the proposed drug on the 3rd day, 1 ml. 3 7.7 ± 0.7 4.2 ± 0.3 10 10.8 ± 0.4 5.5 ± 0.4 thirty 11.2 ± 0.5 5.6 ± 0.6 45 10.8 ± 0.2 5.4 ± 0.7 Group 2 - the introduction of the proposed drug on the 3rd day, 2 ml. 3 7.7 ± 0.1 4.3 ± 0.2 10 11.2 ± 0.3 5.8 ± 0.5 thirty 11.8 ± 0.4 5.9 ± 0.2 45 11.2 ± 0.3 5.8 ± 0.3 Group 3 - the introduction of ferroglukin on the 3rd day, 2 ml. 3 7.8 ± 0.2 4.3 ± 0.3 10 9.6 ± 0.6 5.2 ± 0.4 thirty 10.5 ± 0.9 5.3 ± 0.5 45 9.8 ± 0.7 5.2 ± 0.4 4 group - control (without the introduction of iron-containing drugs) 3 7.6 ± 0.4 4.2 ± 0.4 10 6.2 ± 0.2 3.5 ± 0.1 thirty 4.6 ± 0.1 3.0 ± 0.1

As can be seen from the data in Table 4, without an injection of iron-containing preparations, piglets (group 4) developed severe anemia, and on the 10th day after their birth, the hemoglobin level in the blood decreased from 7.6 ± 0.4 to 6.2 ± 0.2 g% and red blood cells from 4.2 ± 0.4 to 3.5 ± 0.1 × 10 ¹² in 1 liter, and on the 30th day there was a further decrease in hemoglobin to 4.6 ± 0.12% and red blood cells - up to 3.0 ± 0.1-10 g / l.

The introduction of iron-containing drugs prevented the development of anemia. In this case, the indicators in the first and second groups (the proposed drug) were relatively higher compared with the third group (ferroglukin) in all the study periods, although in all animals that received an injection of both iron-containing preparations, they corresponded to the physiological norm.

Animals of the 4th group, in which on the 30th birthday there was severe anemia, were injected twice with 2 ml of the test drug with an interval of 5 days. Hematological studies of these animals 14 days after the first injection of the drug showed that the content of hemoglobin and red blood cells increased, reaching 9.6 ± 0.22% and 5.0 ± 0.1 × 10 ¹² / l, respectively. Thus, experience has shown that the proposed drug not only prevents anemia, but also has a pronounced therapeutic effect, even against the background of developed anemia.

Example 6. Two identical groups of newborn piglets were injected twice with the proposed drug (1 group) and ferroglukin (2 group - control) 1 ml with an interval of 7 days. The conditions for keeping and feeding the piglets were similar. Weaning was performed at two months of age. The results of the experiment are given in table.5.

By weaning, the average live weight of piglets of the 1st group was 19.75 kg, and in the second group - 16.2 kg (Table 5). Therefore, each piglet that received the proposed drug, by weaning was 3.55 kg more compared to the control group.

The economic effect amounted to 180 rubles per 1 dose of the drug.

Table 5
The effect of aminoferrodex on the growth of piglets and the economic effect Group of piglets The average live weight of 1 pig Economic effect per 1 dose of the drug (rub) initial (g) weaning (kg) 1. Experienced (introduction of the proposed drug) 1032 19.75 180 2. Control (administration of ferroglukin-75) 1042 16,20

The positive effect of the influence of the drug was confirmed with wider production experiments when compared with ursoferran (table 6):

Table 6
The effect of iron-containing drug on growth compared with ursoferran Group of piglets Number of belly. in experience (year) Total live weight at weaning (kg) Average live weight of pigs, (kg) 1 - drug administration one hundred 1653.30 16.7 2 - the introduction of ursoferran-200 one hundred 1394.52 14.7

In the course of this experiment, one piglet fell in the first group, and 5 in the second. Piglets died from non-communicable diseases (upset gastrointestinal tract).

By the age of two months, the total live weight of piglets of the first group was 256.78 kg (11.2%) higher than the indicators of the second group. The average live weight of one pig in the first group was 2 kg higher compared to the second (ursoferran-200).

Economic efficiency at a purchase price of 1 kg of live weight of 53 rubles is 106 rubles per piglet, and taking into account the higher safety of growth in group 1, net profit of 13716 rubles was obtained.

Example 7. To determine the effect of ainoferrodex on the reproductive function of pregnant sows in the amount of 10 animals was injected intramuscularly twice with 10 ml of the proposed drug with an interval of 6 days 1.5 months before the survey. A similar group was administered ferroglukin in the same dose. The research results are given in table.7.

Table 7
The effect of the drug on the quantity and quality of offspring Sow groups Number of animals The average number of offspring (goals) per sow Wed live weight of piglets (g) at birth sows piglets 1. Injection of the proposed drug 10 110 11.0 1,180.0 2. Injection of ferroglukin 10 104 10,4 1130.0

Analyzing the data obtained, it should be noted that after injection of the proposed drug the yield of piglets averaged 11 sows per 1 sow, and only 10.4 in the control group (ferroglukin). The average live weight of 1 pig in the first group was 50 g more compared to the second.

Therefore, the proposed drug contributed to a greater yield of piglets (6 goals) and their large-fruited (an average of 50.0 g more).

Example 8. Newborn calves in the amount of 5 goals at the age of 6-7 days with signs of diarrhea additional to symptomatic treatment were injected with the proposed drug in a dose of 10 ml intramuscularly with an interval of 3 days (first group). Similar 3 calves served as a control, and they were injected with 10 ml of ferroglukin also with an interval of 3 days (second group). In calves of the first group, diarrhea stopped for 2-3 days after the first injection of the proposed drug, and in the second only for 4-5 days. 10 days after the start of treatment of calves with iron-containing preparations, hematological studies were performed. Moreover, in the calves of the first group, the content of hemoglobin was 10.6 ± 0.4 g% and erythrocytes 11.3 ± 0.5 × 10 ¹² in 1 liter of blood, and in the second group, respectively, 9.7 ± 0.4 g% and 10.2 ± 0.7 × 10 ¹² in 1 liter of blood. By the age of 1 month, the average live weight of calves in the first group was 49.1 ± 0.8 kg, and in the second - 44.7 ± 0.6 kg. Therefore, the proposed drug, stabilizing the metabolism, improved hematopoiesis and contributed to a faster recovery of calves and their better growth.

Thus, the studies showed that the proposed drug effectively prevents anemia, stimulates hematopoiesis in farm animals, normalizes metabolism and, as a result, higher live weight gain in young animals is achieved, as well as increased yield and safety of the offspring.

The technical and economic effectiveness of the proposed drug according to the data obtained in scientific and industrial experiments, is 106-180 rubles per 1 dose of the drug.

Claims (1)

A drug for the prevention and treatment of anemia, the correction of metabolic disorders in farm animals, including an iron-containing compound, characterized in that it additionally contains starch, copper sulfate, manganese sulfate, pancreatic casein hydrolyzate, and iron trichloride as an iron-containing compound in the following ratio of components , wt.%: Iron trichloride 3.0-3.5 Starch 16.0-18.0 Copper sulfate 0.005-0006 Manganese Sulfate 0.005-0.006 Casein Pancreatic Hydrolyzate 1.2-1.5 Water Rest