RU2351345C1 - Method of obtaining medication for treating diarrhea in farm animals, medication for treating diarrhia in farm animals and method of treating diarrhia - Google Patents

Abstract

FIELD: veterinary.

SUBSTANCE: invention relates to field of veterinary. Method of obtaining medication lies in biological raw material homogenisation, its further water extraction and separation of supernatant by centrifuging. As biological raw material tissues of thymus, spleen and mesenterial lymph nodes of pigs are used. Then in separate glass vessels homogenate of thymus tissue is diluted with 0.85% sodium chloride solution with ratio 1:10, spleen tissue homogenate is diluted with 0.85% sodium chloride solution with ratio 1:5, and homogenate of lymph node tissue is diluted with 0.85% sodium chloride solution with ratio 1:10. Extraction is carried out during 24 hours at temperature 4°C and pH value 7.0. Centrifuging is carried out during 10 minutes at 1500g and temperature 1.5°C. Obtained supernatants are mixed together with ratio 1:1:1, poured into 100 ml vessels and lyophilised. Medication represents mixture of lyophilically dried extracts of thymus, spleen and mesenterial lymph nodes of pig, obtained in accordance with claimed method. Method of treatment of piglets lies in peroral introduction to animals of 25-30 ml of medication, dissolved in 1.5% solution of sodium chloride.

EFFECT: group of inventions has high efficiency in diarrhea treatment.

3 cl, 1 tbl, 1 ex

Description

The invention relates to the field of veterinary medicine, in particular to drugs used to treat gastrointestinal disorders in farm animals - milk pigs and weaned piglets.

Currently, diarrhea is considered an infectious disease that develops in the gastrointestinal tract and requires special medications for its treatment, primarily antibiotics and a number of other drugs. However, the use of antibiotics in veterinary practice has a number of significant drawbacks, in particular the length of the course of their use (usually 5-7 days), the high cost of treatment and a large number of side effects. In addition, it is common for antibiotics to weaken the reproductive function of many animal species, to inhibit the functional activity of the body's immune system. In addition, some antibiotics are poorly absorbed into the blood from the gastrointestinal tract when used internally or are individually poorly tolerated.

In this regard, since 2006, the use of antibiotics for the treatment of diarrheal syndrome has been officially prohibited in the EU countries, and the solution to this problem is carried out by culling sick animals.

A known drug for the treatment of diarrhea of newborn piglets containing as an active principle a substance of the nitrofuran series, in particular semicarbazone-5-nitrofurfural (RF Patent 2075971, A61K 31/00), which is applied to the skin of the mammary gland of a sow on the second or third day after farrowing 2-3 hours before feeding piglets for 2 days, 2 times a day. The disadvantage of this drug is the complexity and the complexity of the method of its use, as well as the high cost of time, which are inevitable when using this drug in large pig farms.

Known means for the treatment and prevention of gastrointestinal diseases of various etiologies with diarrhea syndrome of farm animals, including the active substance is a derivative of benzimidazole and auxiliary components (RF Patent 2128994, A61K 31/41). The disadvantage of this drug is that it lacks the ability to increase antimicrobial resistance and quickly restore the balance of micro and macro elements in the body.

Drugs for the treatment of diarrhea of newborn piglets are also known - antibiotics ampicillin and tetracycline and methods for their use (Guidelines for the prevention and treatment of gastrointestinal diseases in newborn piglets. All-Russian Research Institute of Non-communicable Animal Diseases. Voronezh, 1988, p.17). The disadvantage of using these drugs and the method of their use is that they are administered directly to each piglet, these drugs are ineffective and do not lead to the full recovery of sick animals. The course of treatment for piglets with these drugs is long and lasts at least 5-6 days. A fairly common side effect of antibiotic treatment is their overdose.

The closest in technical essence to the claimed invention is a veterinary preparation for the treatment of diseases of farm animals of bacterial etiology, which is a mother liquor of sodium humate and contains proteins, lipids, minerals, amino acids and enzymes (RF Patent 2214253, A61K 35/10). The main disadvantage of this drug is the duration of its use due to low therapeutic efficacy.

The closest analogue to the method of obtaining the claimed funds is a method of obtaining a medicinal product "Squaacan" containing an extract of the liver of a shark Katran (RF Patent 2211700, A61K 35/60). The main difference between this method and the proposed one is that the extraction of biological substances in it is carried out at acidic pH values.

The objective of the present invention is to provide an agent for treating diarrhea in farm animals that would be free of the above disadvantages, have high specific activity, have immunocorrective effects, and its use would quickly increase the effectiveness of the treatment of diarrhea syndrome in dairy pigs and weaned piglets. In addition, the present invention is the expansion of the existing arsenal of veterinary drugs aimed at the treatment of diarrheal syndrome.

This object is achieved in that the agent for treating diarrhea in farm animals is obtained from the waste of the meat industry, namely from the tissues of the immunocompetent organs of pigs - the thymus, spleen and mesenteric lymph nodes. For this, the tissues of the above organs are cleaned of associated tissues, mechanically crushed and homogenized in a 0.85% sodium chloride solution (saline), after which the tissue homogenates are diluted with saline in separate glass containers of 10 l in the following weight-volume ratios: thymus tissue homogenate / saline - 1/10, homogenate of spleen tissue / saline - 1/5, homogenate of tissue of lymph nodes / saline - 1/10. Aqueous extraction of biologically active substances is carried out for 24 hours at a temperature of 4 ° C and a pH value of 7.0. After that, the homogenates are centrifuged for 10 min at 1500 g and a temperature of 1.5 ° C, the obtained supernatants are mixed with each other in a ratio of 1: 1: 1, poured into 100 ml containers and lyophilized.

The product thus obtained is an amino acid-peptide complex that contains peptides, polypeptides and proteins in a ratio of 12: 9: 16. The authors of the present invention determined that the amino acid composition of the claimed drug is represented by 17 amino acids, the dominant of which, and therefore significantly affect its properties, are aspartic and glutamic acids, alanine, glycine and lysine (Table 1).

Table 1 Amino acid composition of the proposed drug "Dinormin" Amino acids The amino acid content% mm. Arginine 0.62 Aspartic acid 13.77 Alanine 10.24 Valine 4.97 Histidine 1,63 Glutamic acid 15.30 Glycine 10.11 Isoleucine 3.37 Leucine 6.12 Lysine 6.38 Methionine 0.37 Proline 4.21 Serine 5.99 Tyrosine 0.29 Threonine 3.94 Phenyl alanine 3.12 Cysteine 0.31

It was additionally established that of substances with a molecular weight of up to 10,000 D, the composition of the product contains lymphopeptides and their biotransformation products, as well as T-actin, from alpha and gamma interferons, cytochrome C, hyaluronidase, fibrinolysin and “ballast” proteins.

To determine the activity and effectiveness of the claimed funds, experiments were conducted on 44 diarrhea suffering pigs of different ages of both sexes. The animals were divided into 4 groups - two control (10 weaners and 10 milk pigs each) and 2 experimental (12 weaners and 12 milk pigs each), which received the Dinormin® product once orally with a rubber bulb in a volume of 25 -30 ml.

A study of the therapeutic activity of the drug showed that control animals with diarrhea without treatment developed dehydration, weight loss, impaired appetite and 100% death of animals in both groups: in milk piglets by 3-5 days, and in weaners on 10-15 days of illness. When using the Dinormin agent, 83% of milk pigs and 42% of weaners fully recovered. From the above data it is seen that the tool "Dinormin" has pronounced therapeutic antidiarrheal properties.

Also, Dinormin was used to treat diarrhea of weaned calves. The first control group (without treatment) consisted of 12 animals, and the second - 18. During the observation, it was found that in the first control group 10 individuals died or 84%, and in the second - 13 calves or 73% of the animals. Thus, the average mortality rate was 78.5%. In contrast, in experimental calves treated with Dinormin according to the above scheme, the results were different. In the first group of 10 individuals, all animals survived and recovered, and in the second group of 21 individuals, 18 calves survived, thus the mortality rate was 14.3%, and the average mortality rate was only 7.15%. Therefore, the lethality of animals in the experimental group did not exceed 15%, while in the control group this indicator was more than 73%. These data show the high efficiency of Dinormin in the treatment of the bacterial-enzymatic form of diarrhea not only in weaned piglets, but also in calves. This is evidenced by the achievement of not only an antidiarrheal effect, but also an increase in the survival rate of experimental animals.

The method of obtaining funds "Dinormin" is illustrated by a specific example.

Example. After the industrial slaughter of female pigs aged 9-11 months weighing 90-110 kg, slaughtered animals are removed immunocompetent organs - thymus, spleen and mesenteric lymph nodes. Then 1 kg of thymus tissue, 2 kg of spleen tissue and 1 kg of lymph node tissue are cleaned of associated tissues, each tissue is mechanically crushed and homogenized separately in a 0.85% sodium chloride solution. Further, the homogenates of the organs are diluted with a 0.85% sodium chloride solution in separate glass containers of 10 l in the following weight ratios: thymus tissue homogenate / 0.85% sodium chloride solution - 1/10, spleen tissue homogenate / 0.85% sodium chloride solution - 1/5, homogenate of the lymph node tissue / 0.85% sodium chloride solution - 1/10. Aqueous extraction of biologically active substances is carried out for 24 hours at a temperature of 4 ° C and a pH value of 7.0. After that, the homogenates are centrifuged for 10 min at 1500 g and a temperature of 1.5 ° C, the obtained supernatants are mixed with each other in a ratio of 1: 1: 1, poured into 100 ml containers and lyophilized.

Claims (3)

1. The method of obtaining funds for the treatment of diarrhea in farm animals, which consists in the homogenization of bio-raw materials, its subsequent aqueous extraction and separation of the supernatant by centrifugation, characterized in that tissues of the thymus, spleen and mesenteric lymph nodes of pigs are used as bio-materials, then in separate glass containers the thymus tissue homogenate is diluted with a 0.85% sodium chloride solution in a ratio of 1:10, the spleen tissue homogenate is diluted with a 0.85% sodium chloride solution in a ratio of 1: 5, and homogenous tissue of lymph nodes is diluted with a 0.85% sodium chloride solution in a ratio of 1:10, extraction is carried out for 24 hours at a temperature of 4 ° C and a pH value of 7.0, centrifugation is carried out for 10 minutes at 1500 g and a temperature of 1.5 ° C, the resulting supernatants are mixed with each other in a 1: 1: 1 ratio, poured into 100 ml containers and lyophilized. 2. An agent for treating diarrhea in farm animals, characterized in that it is a mixture of freeze-dried extracts of thymus, spleen and pig mesenteric lymph nodes obtained according to claim 1. 3. A method of treating diarrhea in piglets, characterized in that the animals are orally administered 25-30 ml of the drug according to claim 2, dissolved in a 1.5% sodium chloride solution.