RU2342857C2 - Dietary supplement "migikalgin" - Google Patents

Abstract

FIELD: food products.

SUBSTANCE: dietary supplement contains calcium alginate and protein hydrolyzate from molluscs meat in the following amount: calcium alginate - 210.5-280.0, protein hydrolyzate from molluscs meat - 190-240.0 in weight fractions.

EFFECT: product with polyfunctional properties based on enterosorbent and calcium source and nitrogen-containing components, in maximal available form for an organism and with increased biological activity.

2 cl, 4 tbl

Description

The invention relates to biologically active food additives obtained by processing water objects, such as algae and mollusks.

Known biologically active food supplement containing an adsorbent and kelp extract (see RF patent No. 2164144, A61K 35/80, 2000). The drug is intended for the prevention and treatment of various diseases caused by iodine deficiency in the body.

A known product of mussel meat, which is a protein hydrolyzate of black sea mussel meat (see RF patent No. 2017439, A23L 1/30, 1992), which has the ability to increase human resistance.

The closest technical solution to the claimed one is a biologically active additive containing calcium alginate and a preparation from marine brown algae (see application No. 2002120548/15, А61К 35/80, 2002).

In addition, the additive contains additional substances that improve the taste and consumer properties. The supplement is intended for the prevention and treatment of osteoporosis.

Known additives have positive results when used. The technical task of the claimed invention is to obtain a product with multifunctional properties in the form most accessible to the body.

The problem is solved in a biologically active food supplement containing calcium alginate, as well as protein hydrolyzate from mollusk meat in the amount of parts by weight:

calcium alginate 210.5-280.0,

protein hydrolyzate from mollusk meat 190-240.0.

In addition, a dietary supplement contains ascorbic acid and glucose.

One of the promising biomedical directions is the preparation of a new generation of preparations with multifunctional properties based on calcium alginate - an enterosorbent and a calcium source, as well as nitrogen-containing components - amino acids, oligopeptides and macro- and microelements that are part of the protein hydrolyzate from mollusk meat, for example, from mussel meat Mytilus edulis, a food product. Hydrolysates of mussel meat are a mixture of irreplaceable and non-replaceable easily digestible amino acids, peptides, melanoidins, minerals (macro- and micronutrients) and lipids (which include unsaturated fatty acids, including a group of polyunsaturated fatty acids (omega-3 and omega-6).

Alginates - dietary fiber, salts of alginic acid, which are enterosorbents. Alginic acid is a polysaccharide consisting of residues of D-mannuric and L-guluronic acids. Calcium alginate is a calcium salt of alginic acid with sorption properties obtained during the processing of algae in the presence of calcium salts.

Derivatives of alginic acid of brown algae - alginates are widely used to create emulsion, gel-like, structured foods, in medicine, and also as a source of dietary fiber. However, in this composition, calcium alginate with a protein hydrolyzate from mollusk meat exhibits not only previously known properties, but also in the process of producing dietary supplements provides the release of amino acids from the hydrolyzate in a free, easily digestible form. This combination of these components has not been used previously, in addition, in this case, a synergistic effect is manifested. As for the quantitative ratio, it was found that only with this ratio the most maximum yield of amino acids is obtained.

Migikalgin biologically active food supplement is made by extraction of alginate from brown algae of the laminaria family, obtaining a calcium alginate gel during its precipitation with a solution of calcium chloride from an alginate extract, separating excess liquid by pressing (up to 80% in a pressed gel) and adsorption to the surface of the crude calcium alginate protein hydrolyzate from mollusk meat, followed by drying the mixture by heat drying or freeze-drying. Moreover, the preparation of a new preparation by this method eliminates the stage of drying an intermediate product - calcium alginate.

Manufacturing process

Migikalgin biologically active food supplement provides for the use of ready-made forms of calcium alginate produced by industrial enterprises. The chemical composition of migikalgin is presented in table 1.

Table 1.
The chemical composition of dietary supplements "Migikalgina" and "Migikalgina-S '' Product name Content% Amount of amino acids from, mg / 100 g water dry matter alginic acid total nitrogen mineral substances Migicalgin 4.03 95.97 28.0-34.0 2.76 47.00 669.1 Migikalgin-S 6.68 93.32 28.0-34.0 2,51 41.45 883.1

Due to the adsorption on calcium alginate of a protein hydrolyzate from mussel meat, the Migikalgin dietary supplement contains, in addition to calcium alginate (a salt of alginic acid), a complete set of amino acids in a free digestible form (Table 2), as well as micro and macro elements - calcium, potassium, iron, molybdenum, zinc, etc.

Table 2.
Amino acid composition of Migikalgin and Migikalgin-S, mg / 100 g Amino acid “Migikalgin” Migikalgin-S Aspartic acid 74.0 102.9 Serine 48.5 53.3 Glutamic acid 91.7 141.1 Glycine 89.6 70.3 Alanine 57.6 53.6 Histidine 4.4 7.2 Arginine 41.6 88.6 Proline 30.7 36.9 Isoleucine 23.3 32,0 Leucine 32,5 44.6 Methionine 13.6 21,2 Cystine 3.2 8.0 Phenylalanine 12.8 22.1 Tyrosine 4,5 8.5 Threonine 39.2 48.7 Valine 32,4 39.6 Lysine 54.5 83.3 Oxyproline 4.0 5.5 Oxylysine 1.3 2.1 Ornithine 2.2 3,7 Taurine 7.5 9.8

Thus, the dietary supplement "Migikalgin" is a complete source of dietary fiber (calcium alginate), free amino acids, a complete set of biogenic micro and macro elements and can be recommended for use as a source of biologically active substances.

The protein hydrolyzate from mussel meat, adsorbed on alginate and turned into a dry state, is the basis that combines well with ascorbic acid and glucose. In addition, the new form of dietary supplement "Migikalgin-S" (capsules) is more convenient for transportation and use as a dietary supplement.

To expand the scope and facilitate the removal of calcium from calcium alginate, its translation into an ionized form and assimilation by the body, ascorbic acid and glucose are additionally added to the composition.

The basis for the introduction of ascorbic acid in dietary supplements is the evidence that it is one of the most powerful antioxidants that can block free radical processes in the body and is involved in the regulation of protein metabolism. A sufficient content of ascorbic acid in the diet contributes to the most complete creation of glycogen stores in the liver and an increase in its antitoxic function. In addition, participating in lipid metabolism, ascorbic acid normalizes blood cholesterol and delays the development of atherosclerosis. Of particular interest is the participation of ascorbic acid in immunological reactions and the maintenance of nonspecific immunoreactivity of the body.

The argument in favor of introducing glucose into the dietary supplement is that it is the only carbohydrate circulating in the blood and providing the energy needs of the body, and first of all, the central nervous system. Thus, these data were the basis for the development of the Migikalgin-S dietary supplement formulation (Table 3 — examples).

Table 3.
Examples of formulations dietary supplements "Migikalgin" and dietary supplements "Migikalgin-S" Name of components Dietary supplement Migikalgin BAA Migikalgin-S Clam Meat Protein Hydrolyzate 190 200 215 230 195 200 220 Calcium alginate 280 210.5 250 230 245 212 260 Glucose 144 140 135 Vitamin C twenty eighteen 25

Studies to determine the biological activity and the study of the chronic toxicity of dietary supplements "Migikalgina" and "Migikalgina-S" were carried out at the Medical Radiological Scientific Center of RAMS.

The experiments on the study of chronic toxicity of Migikalgin were performed on two types of laboratory animals: F1 hybrid mice (CBA × C57B1 / 6) and Wistar rats, males 3 months old in mice and 2.5 months old in rats, weighing at the beginning experiment about 20 g (mouse) and 140 g (rat) contained in normal nutritional conditions of laboratory vivarium. 15 mice and rats were in two control groups (these animals were slaughtered 4 months after the start of the experiment) and 15 animals were in 4 experimental groups, which were given 5 ml daily of 1 kg of animal body weight through a gastric tube for 1 month 10% suspension of Migicalgin or Migicalgin-C in distilled water. The animals of the experimental groups were slaughtered 4 months after the start of the experiment or 3 months after the completion of the drug administration. In total, 90 animals were used in the experiment, i.e. 45 animals of each species. During the experiment, animals were periodically weighed, taking into account their general condition, activity, appearance, condition of the coat. After slaughter, rats were taken material for histological examination of tissues, and mice were used to study the state of hematopoietic systems and immunity according to the morphological composition of blood and bone marrow.

Morphological and hematological studies were carried out in accordance with generally accepted experimental methods.

The results showed that animals of both species, without apparent negative consequences, suffered a monthly consumption of dietary supplements Migikalgin and Migikalgin-S. The animals of the experimental groups did not differ from the animals of the control groups in behavior, appearance and condition of the coat, as well as in body weight.

Pathomorphological studies revealed no deviations in the state of internal organs from the norm. When opening animals slaughtered 4 months after the start of the experiment, no macroscopic differences were found in the organs and tissues of the control and experimental animals. The macroscopic picture was the same. All animals were of the correct physique, good fatness, the visible mucous membranes were moist, shiny, pale pink.

When opening the thoracic and abdominal cavities, deviations from the norm in the anatomical and topographic arrangement of organs and their sizes were not established

Studies to determine the biological (hemostimulating and radioprotective) activity of dietary supplements Migikalgin and Migikalgin-C were performed on F / (CBA × C57B1 / 6) hybrid mice (Table 4). The studied additives were administered orally to mice in the form of a 10% suspension in distilled water at the rate of 5 ml / kg body weight for 4 days, then the animals were subjected to gamma irradiation at a dose of 6 Gy. Eight days after the irradiation, the animals were sacrificed, the spleens were weighed, and the number of endocolonies formed by hematopoietic stem cells in response to dietary supplements was counted on their surface.

Table 4.
Biological activity of dietary supplements "Migikalgin" and "Migikalgin-S" Group The average mass of the spleen (M ± t), mg The average number of endocolonies (M ± t) Migikalgin 56.8 ± 4.0 4.1 ± 0.5 Migikalgin-S 50.3 ± 3.8 3.2 ± 0.5 Control (vivar diet) 6 Gy 44.6 ± 2.9 1.9 ± 0.3

Clinical trials were conducted for 3 months at the Russian Scientific Center for Restorative Medicine and Balneology in Moscow. The tests were carried out on the basis of the departments for the treatment of gastrointestinal diseases, cardiovascular, endocrinological and rehabilitation treatment in the experimental and control groups of patients with a population of at least 14 people. It was established that dietary supplements "Migikalgin" and "Migikalgin-S" are well tolerated by patients, do not cause allergic reactions. Based on the results, preliminary recommendations on the use of dietary supplements "Migikalgin" and "Migikalgin-C" have been developed.

Claims (2)

1. Biologically active food supplement containing calcium alginate, characterized in that it further comprises a protein hydrolyzate from mollusk meat in the amount of parts by weight:
calcium alginate 210.5-280.0 clam meat protein hydrolyzate 190-240.0 2. A biologically active food supplement according to claim 1, characterized in that it further comprises ascorbic acid and glucose.