RU2404788C2 - Composition with general health-improving effect - Google Patents

Abstract

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to chemical-pharmaceutical industry, in particular to development of composition with general health-improving effect. Composition with general health-improving effect on the basis of herbal raw material, including inulin and pectin, additionally contains proteins, amino acids, macro- and microelements, and also malic, malonic, fumaric, citric and succinic acids taken at a certain ratio.

EFFECT: invention makes it possible to produce efficient general health-improving action at human and animal organism in case of various acute respiratory virus infection symptoms and diabetes mellitus.

2 cl, 5 tbl, 6 ex

Description

The invention relates to the field of biotechnology, in particular to the technology of using a composition of biologically active substances (BAS or biologically active components - LHC), and can find application in preventive medicine, the food industry, in the production of feed and feed additives for domestic and farm animals, as physiologically active biogenic component in cosmetics, in particular shampoos, lotions, creams.

In recent years, more and more attention has been paid to the formation of a healthy lifestyle, the prevention of socially significant diseases, and also to improving the quality of life and its duration. Undoubtedly, the concept of a “healthy lifestyle” is multicomponent and includes, by definition, a set of measures that ensure the harmonious development and strengthening of health, increase the working capacity of people, and prolong their longevity. An important role in this set of measures is currently played by the possibility of using the results of biotechnological research and discoveries. Biotechnology is the industrial use of biological agents (microorganisms, plant cells, animal cells, parts of cells: cell membranes, ribosomes, mitochondria, chloroplasts, etc.) to produce valuable products and carry out targeted transformations.

In the framework of the present invention, we will consider the possibility of using biotechnological transformations (biotechnological conversion of plant materials) in order to obtain a composition that has a general strengthening effect that can be used in the food industry, preventive medicine, the production of feed and feed additives, and in the production of perfumes and cosmetics.

The prior art knows a fairly large number of plants that can be used to obtain substances that are biologically active and have a wide spectrum of action, including plants of interest to us in the framework of the present invention, that is, containing substances suitable for creating a composition having restorative action, in particular inulin-containing plants. Such plants include, for example, Jerusalem artichoke, chicory, dandelion, leeks and several others.

It is known from the prior art that Jerusalem artichoke (Jerusalem artichoke) contains a number of biologically active components (LHC), of which the following are significant for the realization of our purpose, namely the creation of a composition with a tonic effect:

- inulin;

- pectin;

- phosphorus;

- potassium;

- iron;

- silicon;

- zinc;

- manganese;

- calcium;

- vitamins, in particular B2, B1, PP, C;

- nitrogenous substances, including protein, amino acids (about 16 amino acids, 8 of which are not synthesized in the human body, that is, they are “irreplaceable AKs”);

- carboxylic acids, in particular malic, succinic, citric malonic, fumaric acids,

as well as a number of other LHC.

It should be noted that each of the above LHCs has not one, but a whole spectrum of properties and actions on the human and animal organism, which can be combined using the term “general strengthening effect”.

So, for example, it is known that inulin has a beneficial effect during the entire time spent in the human body - from getting into the stomach and ending with secretion. Inulin, getting into the gastrointestinal tract, is split by hydrochloric acid and enzymes into individual fructose molecules and short fructose chains that penetrate into the bloodstream. The remaining undigested portion is rapidly excreted, linking a large amount of substances unnecessary to the body, such as heavy metals, radionuclides, cholesterol crystals, fatty acids, various toxic chemical compounds that enter the body with food or are formed during the life of pathogenic microbes living in the intestines. In addition, inulin significantly stimulates the contractility of the intestinal wall, which significantly accelerates the cleansing of the body of toxins, undigested food and harmful substances. The antitoxic effect of inulin is enhanced by the action of pectin, also found in Jerusalem artichoke, chicory, dandelion, etc. Short fructose chains that are absorbed in the intestine and in the blood continue to perform an antitoxic, cleansing function, linking, neutralizing, and facilitating the excretion of harmful metabolic products and from the external environment of chemical compounds. The action of inulin also manifests itself in the modification of intestinal microflora by promoting the development of Bifidus group bacteria. In addition, inulin reduces the level of glucose in the blood of diabetics, improves lipid metabolism, thereby preventing the occurrence of complications of diabetes mellitus (retinopathy, angiopathy, etc.); reduces blood cholesterol, triglycerides and phospholipids, helping to reduce the risk of cardiovascular disease; counteracts the development of cancer, in particular immunogenic tumors; It has an immunomodulatory and hematoprotective effect; improves the digestibility of vitamins and minerals in the body (especially Ca, Mg, Zn, Cu, Fe and P); has an effect on bone tissue (including due to improved absorption of minerals), reducing the effects of osteoporosis.

Pectin also has a smaller spectrum of action on the human and animal organism. So, pectin, being a high molecular weight carbohydrate present in the intercellular substance and walls of plant cells, has adsorbing, astringent and gelling properties; able to reduce cholesterol in the body, helps to improve metabolic processes, normalizes intestinal motility, improves peripheral blood circulation.

In addition, it should be borne in mind that tubers and stems (aerial parts) of Jerusalem artichoke, from which, in accordance with our proposed method, it is possible to obtain a composition with a general strengthening effect, practically do not accumulate nitrates, heavy metals, and radioactive elements in them. This explains the high safety of the use of Jerusalem artichoke components for food, medical, cosmetic and other purposes.

When analyzing the state of the art, we analyzed patent and non-patent sources containing information on methods for producing LHC that are part of various, inulin-containing, plants, compositions made on the basis of the obtained raw materials, and areas of their application.

Thus, a composition is known, which is essentially a powder of dried Jerusalem artichoke tubers, containing at least one salt of aspartic acid with a metal cation selected from the group of macro- and micronutrients (RU 2140171 C1, 10.27.1999). However, the disadvantage of such a composition is the high content of “ballast” substances in it, such as cellulose, hemicellulose, lignin, the presence of which in the composition leads to a decrease in absorption in the human body and animals of the LHC contained in the composition, a decrease in their absorption, the occurrence of flatulence.

The closest to the composition we have proposed, we consider a composition that has a therapeutic and prophylactic effect, which can clearly be attributed to a restorative effect described in the source RU 2169002 C2, 06/20/2001. This composition consists of pectin and inulin obtained from dried tubers of Jerusalem artichoke. The ingredients of this composition are obtained by aqueous extraction of the inulin fraction (first stage) and subsequent extraction of pectin (second stage) from the remaining raw materials using an aqueous acid solution. Then, each of the obtained ingredients of the composition is crystallized and precipitated, after which it is mixed and ground to obtain the final product. In our opinion, the main disadvantages of such a composition are the following:

- the use in the composition of only part of the LHC contained in plant materials;

- the use of inulin and pectin in a ratio of 2: 1, as a result of which the value of such a composition when used in the food industry, feed, preventive medicine is significantly reduced compared to, for example, using a powder of dried plant materials (chicory, Jerusalem artichoke);

- deterioration in the functional properties of the composition due to the degradation of polymers occurring during drying of the raw material.

We have proposed a composition that can be obtained from any plant material, including the ingredients required for composing the composition, by any known method that makes it possible to extract these ingredients from the specified raw materials, and having the following qualitative and quantitative compositions, which can have a general strengthening effect on the human and animal body:

Inulin - 70-85 wt.%;

Pectin - 11-3 wt.%;

Nitrous in-va 9-3 wt.%

Mineral Islands 3.8-3.5 wt.%;

Carboxylic acids 6.2-5.5 wt.% In terms of dry matter (the content of nitrogenous substances was determined by Kjeldahl (N × 6.25)). The nitrogenous substances that make up the composition are represented by a protein of 3.3-1.1 wt.% And amino acids, namely:

Table 1 Amino acids, wt.% Sv: Asparagine 0.43-0.16 Glutamine 0.34-0.11 Serine 0.15-0.05 Histidine 0.46-0.16 Glycine 0.19-0.06 Threonine 0.05-0.02 Alanine 0.10-0.03 Arginine 0.97-0.32 Tyrosine 0.58-0.19 Cystine 0.87-0.29 Valine 0.19-0.06 Methionine 0.10-0.03 Tryptophan 0.15-0.05 Isoleucine 0.15-0.05 Leucine 0.58-0.19 Lysine 0.39-0.13

As mineral substances in the composition contain:

table 2 Minerals, wt.% Sv: Potassium 2.92-3.17 Calcium 0.0834-0.0906 Magnesium 0,089-0,096 Sodium 0,043-0,047 Sulfur 0.0065-0.0072 Phosphorus 0.348-0.378 Iron 0.0037-0.004 Chromium 0.0045-0.005 Copper 0,00025-0,0003 Manganese 0,00025-0,0003 Zinc 0.0014-0.0016

As carboxylic acids, the composition contains:

malic acid 3.2-2.6 wt.%, malonic 1.1-1.7 wt.%, fumaric 0.9-1.3 wt.%, citric 0.12-0.24 wt.%, succinic 0.18-0.36 wt.%.

The specified composition can be obtained from any plant material containing one or more ingredients, for example, chicory, Jerusalem artichoke, etc.

As a method for preparing the composition, any method known to those skilled in the art can be used, including aqueous extraction using acids or enzymes, or electrophysical methods.

Methods for producing inulin, pectin and other LHC, as well as the raw materials necessary for producing, are widely known to those skilled in the art.

Regardless of the method of preparation and the raw materials used, the composition obtained in the indicated qualitative and quantitative composition will exhibit a general strengthening effect when used.

The technical result achieved when using this composition is the ability to exert a general strengthening effect on the human and animal body both through the use of the ingredients of the composition themselves and through the use of certain amounts of each of the ingredients. Within the framework of our task, the selected qualitative composition of the composition affects the ability to achieve a general strengthening effect due to the inclusion of amino acids, including essential, vitamins, microelements, in the composition of the composition. The quantitative content of ingredients within the declared range makes it possible to provide the human and animal body with substances necessary for functioning (LHC) and achieve a general strengthening effect within the framework of more narrowly set tasks - for the purpose of prevention in healthy subjects, in complex treatment for various diseases, etc. In addition, the qualitative composition and claimed ranges of quantitative values of the ingredients of the composition are interconnected. Thus, the addition of another ingredient, pectin, to the composition, in addition to inulin, allows using the composition not to exceed the admissible level of inulin recommended for consumption due to the fact that inulin and pectin contribute to the mutual enhancement of the action (for example, sorbing); on the other hand, the quantitative predominance in the composition of inulin ensures the predominance of the sorbing effect on the stimulation of intestinal motility, etc.

I would also like to dwell in more detail on what should be understood by the “restorative effect” that this composition has. As mentioned earlier, each of the ingredients of the composition has a number of properties that can have a positive effect on the state of organs and systems of the human or animal body. However, the possibility of using a composition that includes all of the above ingredients provides a result that is not only a simple sum of the effects provided by each of the biologically active substances (components) included in its composition, but also a consequence of the mutual influence of the ingredients on the processes of absorption, absorption, each other’s metabolism (for example, inulin enhances calcium resorption by bone tissue, etc.). The general strengthening effect itself consists of immunostimulating, detoxifying, anticarcinogenic, antioxidant, etc. Moreover, the point of application of the action of the composition may be, in particular, human skin; at the same time, the general strengthening effect will be exerted precisely on the skin and will consist in increasing the skin tone and turgor, local immunity (protective function of the skin).

A composition having such a wide spectrum of action, indicated by the term “restorative effect” adopted in preventive medicine, can be used:

- in the health sector for the manufacture of biologically active additives that can be used in the complex treatment of patients with diseases of the gastrointestinal tract, diabetes mellitus, arterial hypertension, metabolic syndrome, decreased immunity, age-related diseases of the bone tissue (osteoporosis, osteopenia), etc. ., as well as in healthy people in order to prevent the above diseases and maintain vitality and activity;

- in the food industry - for enrichment with biologically active components of food products, including bakery and confectionery products, pasta, sausages, dairy products, drinks; to create feeds and feed additives for domestic and farm animals;

- in the manufacture of perfumes and cosmetics for the purpose of their enrichment with biologically active components.

The advantages of using our composition in comparison with previously known ones, in particular compared with the use of tablets of dried Jerusalem artichoke powder known from the prior art, can be demonstrated by our study: during a period of increased epidemiological danger of ARVI, in order to prevent the disease, one group of students consumed daily Jerusalem artichoke powder in quantities corresponding to an adequate level of inulin consumption per day. The second group for this purpose consumed the composition of a general strengthening effect in amounts corresponding to an adequate level of inulin consumption per day. In the group of students who received the composition of a general strengthening effect, the incidence during the entire observation period was 3 times less.

Below we give examples confirming the possibility of using the proposed composition with the stated purpose in various fields of production and in the field of healthcare.

Example 1

 This composition was tested on sick volunteers with an experience of at least 5 years. The study involved two groups of patients: in the experimental group, in addition to drugs, patients received this composition. Almost all regularly taking the composition noted an improvement in their health status: increased working capacity, improved psychoemotional background, decreased fatigue and drowsiness, and improved vision. In addition, at the end of the period of taking the composition in the experimental group of patients with diabetes mellitus, there was a decrease in glycemic profile compared with patients who did not take this composition. Without exception, all those who took the composition noted the convenience of its use, long term and ease of storage. Thus, the effect of using the composition is expressed in the prevention of side diseases and the increased effects of complex therapy in the treatment of diabetes mellitus.

Example 2

In this experiment, cases of ARVI in 14 people of the experimental group and 10 people in the control group for 12 months were analyzed. The experimental group took the composition three times a day, 3 g after meals, and in the control group the composition was not prescribed. ARVI disease was determined by the following symptoms: sore throat, dry cough, headache, myalgia and chills. The experimental results are shown in tables 3 and 4. In the experimental group, there was only one case of a cold, however, this case is associated with the termination of the composition. In the control group, a total of 32 cases of colds were registered, that is, an average of 3.2 times per year per person.

Table 3 The frequency of colds in the experimental group Age Floor Common cold one 63 male 0 2 65 male 0 3 66 female 0 four 78 male 0 5 61 male 0 6 63 male 0 7 64 male 0 8 38 male 0 9 59 female 0 10 64 male one eleven 65 male 0 12 72 male 0 13 41 male 0 fourteen 43 male 0

Table 4 The frequency of colds in the control group Age Floor Common cold one 43 female 3 2 47 female 5 3 39 male four four 44 male four 5 42 female 3 6 46 female four 7 39 male 2 8 38 male 3 9 66 male 2 10 35 female 2

Example 3

This composition was used in the preparation of bread from wheat flour. The composition was made when kneading dough in an amount of 3.5% by weight of flour.

The use of this composition allowed to improve the rheological characteristics of the viscosity test immediately after kneading by 4.0%, and after fermentation by 6%; increase porosity by 5% (finer-walled porosity of the crumb), improve the compressibility of the crumb (instant modulus of elasticity of the crumb of the products is lower by 26), increase the share of firmly bound moisture, which slows down staling of bread for 5-7 hours. The use of this composition can increase the nutritional value and expand the range of dietary varieties of bread.

Example 4

In order to improve the technology of functional boiled sausages, the composition in the amount of 4% was introduced into the recipe composition of boiled sausages from traditional raw materials and meat of mechanical deboned poultry (MMO).

The obtained samples of the finished product met the regulatory requirements for the content of salt and moisture. The yield of finished products at a 4% level of meat substitution by this composition is increased by 2.5% for sausages from MMO and by 4.1% for sausages from traditional raw materials, which is explained by an increase in water binding capacity in experimental samples compared to control ones. The use of this composition helps to improve the fat / protein ratio in sausage, increasing the content of potassium, calcium, magnesium, as well as manganese, copper and zinc. The content of macronutrients in prototypes of sausages increased by 2.9-3.3%, and micronutrients by 4.7-10.8% compared with control samples.

Example 5

 The use of compositions for the production of feed and feed additives. During the year, a veterinary clinic conducted a statistical record of injured (with fractures of limbs) pets. The treatment was carried out by standard methods. In the course of treatment, ready-made feed was used to feed control animals without adding a restorative action composition, and when experimental animals were fed, ready-made feed was used with the addition of a restorative action composition. The introduction of this supplement stimulates bone formation and improves bone regeneration in the treatment of fractures. As a result, recovery of experimental animals accelerated in 98 percent of cases, see table 5.

Table 5 Feed used Recovery Control group of animals Ready-made feed without adding a restorative action composition Within 4-6 weeks Experienced Animal Group Ready-made feed with the addition of a general strengthening effect composition Acceleration by 1-2 weeks in 98% of cases

Example 6

The composition can be introduced into any perfumery-cosmetic base, depending on which cosmetic product you want to create. In this case, the recommended amount of composition in the cosmetic product is from 2 to 5%. A higher concentration increases the density of the cosmetic product, a lower concentration reduces the biological effectiveness of the cosmetic product.

Perfume and cosmetic

Fat-based cream containing (wt.%): Composition - 4, paraffin - 0.5-1.5, olive oil - 0.5-1.5, distilled monoglycerides - 6.5-7.5, beeswax - 0.5-1.5, coconut oil - 1.0-2.0, glycerin - 5.0-6.0, sulfonol 0.05-0.15, sorbic acid 0.15-0.25, caustic potassium - 0.01-0.03, fragrance 0.1-0.2, aqueous extract of Japanese kelp - up to 100.

Tests of cosmetics using the inventive composition, conducted on volunteers (12 people aged 18 to 60 years), showed that they exhibit an increased regenerative effect due to the activation of "skin immunity". Physically and biologically, the skin is cleaned of impurities, while the elasticity, softness and elasticity of the skin are restored.

Thus, the above examples confirm the possibility of using the proposed composition of general strengthening action in various sectors of the food industry, in preventive medicine, in the perfumery and cosmetics industry, in the production of feed and feed additives.

Claims (2)

1. A composition having a general strengthening effect and obtained from plant materials, including inulin, pectin, characterized in that it further contains proteins, amino acids, macro- and microelements, as well as malic, malonic, fumaric, citric and succinic acids, while the content of ingredients in the composition is, wt.%:
inulin 70.0-85.00 pectin 3.00-11.00 squirrels 1.10-3.30 amino acids 1.90-5.70 macrocells 3.40-3.69 trace elements 0.10-0.11 Apple acid 2.60-3.20 malonic acid 1.10-1.70 fumaric acid 0.90-1.30 lemon acid 0.12-0.24 succinic acid 0.18-0.36 2. The composition according to claim 1, characterized in that the amino acid contains, wt.%: Asparagine 0.16-0.43, glutamine 0.11-0.34, serine 0.05-0.15, histidine 0 , 16-0.46, glycine 0.06-0.19, threonine 0.02-0.05, alanine 0.03-0.10, arginine 0.32-0.97, tyrosine 0.19-0, 58, cystine 0.29-0.87, valine 0.06-0.19, methionine 0.03-0.10, tryptophan 0.05-0.15, isoleucine 0.05-0.1 5, leucine 0 19-0.58; lysine 0.13-0.39.