RU2264225C1 - Method of normalizing physiological condition of organism during treatment of toxicosis - Google Patents

Abstract

FIELD: toxicology.

SUBSTANCE: invention relates to treating toxicoses caused by alcoholism or narcomania For that end, vegetable-origin enterosorbent is used, which is administered simultaneously with abdominal decompression.

EFFECT: increased sorption capacity resulting in elevated detoxication effect.

2 cl, 3 tbl, 4 ex

Description

The invention relates to medicine and relates to the normalization of the physiological state of the body. It is most effectively used in the treatment of toxicosis caused by alcoholism or drug addiction.

There is a method of normalizing the physiological state of the body in the treatment of toxicosis caused by the use of alcohol, which involves reducing the activity of aldehyde dehydrogenase to increase the level of acetaldehyde in the blood using forskolin, which leads to a decrease in alcohol consumption (RU 2183455, A 61 K 31/352, A 61 P 25 / 32, 1999; EP 96/01952, 1996; WO 96/36332, 1996; US 5902823, A 61 K 31/35, 35/78, 1999).

This method is preventive and unacceptable in the treatment of acute toxicosis, including those caused by the use of alcohol or drugs.

There is also known a method of normalizing the physiological state of the body in the treatment of drug addiction, providing for drug therapy using drugs based on phenobarbital with simultaneous surface inhalation anesthesia under the influence of oxygen-xenon mixture (RU 2226408, A 61 M 16/12, 2003). It is also possible to carry out hemedesis against the background of symptomatic drug therapy (RU 2181593, A 61 K 31/79, 31/375, 35/78, 33/14, A 61 M 21/00, 2002) or hyperbaric oxygenation and lumbar oxygen puncture (RU 2227004, A 61 G 10/02, A61K 37/79, 33/00, 2002).

However, these methods are difficult to implement and unsafe for the patient.

The main direction of development of ways to normalize the physiological state in the treatment of toxicosis is to cleanse the body with the help of enterosorbents (see, for example: “Enterosorption”, under the editorship of N. Belyaev, L., 1991; US 4614513, A 61 M 1/03, 1/36, 1986), including in combination with an immunomodulator - T-activin (RU 2136295, A 61 K 35/26, 1999). Among the means used for this purpose, preparations based on zeosorb - enterosorbent from natural zeolite-containing tuffs (RU 2145216, A 61 K 31/045, 1/185, 1/19, 1/56, 1/70, 33/00, 2000, were widely used. )

However, this method is ineffective and inconvenient for use due to the insufficient elaboration of the action of enterosorbents of various origin, and the use of T-activin does not correspond to the etiology of the disease.

Closest to the claimed is a method of normalizing the physiological state of the body in the treatment of toxicosis, which involves the introduction of enterosorbents into the body orally in combination with chemotherapeutic drugs, drugs from medicinal plants, hepatotropic drugs, means that normalize the activity of the stomach and intestines, vitamins and minerals, as well as physiotherapeutic effect - electrophoresis, magnetic field, laser radiation to stimulate interstitial humoral transport and fun Ktsi lymphatic system. Moreover, for detoxification therapy using polypepam is an enterosorbent of plant origin (RU 2131727, A 61 K 31/00, 31/07, 31/365, 31/51, 35/12, 35/64, 35/78, A61N 1 / 30, 2/00, 5/06, 1999).

However, this method has low efficiency due to the insufficient sorption activity of the used enterosorbents, including under extreme conditions of the above physiotherapeutic procedures.

The technical task of the proposed method is to increase its physiological effectiveness and expand the arsenal of means used for this purpose.

The solution to this technical problem is that the following change is made to the method of normalizing the physiological state of the body in the treatment of toxicosis by detoxification by oral administration of an enterosorbent of plant origin in combination with a physiotherapeutic effect: abdominal decompression (BP) is performed as a physiotherapeutic effect.

A causal relationship between the changes made and the technical result achieved is an increase in blood flow in the intestinal vessels under blood pressure conditions, which leads to an increase in the influx of endotoxins, their excretion, and sorbent binding. Thus, the proposed method is based on the first established by the authors, previously unknown phenomenon of increasing the sorption activity of enterosorbents of plant origin under the action of proteolytic enzymes of digestive juices in blood pressure conditions.

The claimed distinguishing features characterizing the used sorbents, as well as the conduct of blood pressure in relation to the intended purpose, are new both in aggregate and separately.

During the operation of abdominal decompression, the vacuum is maintained in the generally used range from -0.02 to -0.1 kg / cm ² (RU 2051630, A 61 B 17/42, A 61 M 1/00, 1996).

As a technical means for the implementation of blood pressure can be used apparatus according to patent RU 2143257, A 61 N 9/00; 1999, as well as any other equipment for this purpose, up to sealing the abdominal cavity with a tarpaulin and pumping out air at the specified place of sealing during the control and regulation of rarefaction.

As an enterosorbent of plant origin, it is most advisable to use filtrum or zosterol, which is confirmed by the following examples. These enterosorbents for the treatment of toxicosis have not previously been used. Their known purpose is limited to the treatment of wounds and gastroduodenitis (RU 2171108, A 61 K 31/715, A 61 P 1/00, 2001; RU 2194535, A 61 L 15/28, 15/30, 2002). From this group of enterosorbents, the use of recene is also possible.

Table 1 shows the dynamics of changes in the concentration of echinocytes in rats in the dynamics of treatment of acute poisoning; in table 2 shows the effect of filtrum in combination with and without blood pressure on the immune system of alcoholized rats; table 3 shows the ratio of deformed red blood cells before and after blood pressure in patients with toxicosis of various etiologies,

The method is illustrated by the following examples.

EXAMPLE 1. Experimental groups of Wistar rats (10-30 animals per group) are administered malathion, ethyl alcohol and morphine at a dose of 1 LD ₅₀ per individual, respectively. In addition, examine the control group of rats, which were not administered toxins. To assess the toxic effect, the percentage of deformed red blood cells (echinocytes) is taken into account. Normalization of the physiological state of rats is carried out by oral administration of enterosorbents of plant origin - filterum, zosterin, recene RD, as well as enterosorbents known for this purpose - polypepam (RU 2131727) and activated carbon in combination with abdominal decompression (once for 10 min at dilution values from - 0.02 to -0.20 kg / cm ² ). Dosage of enterosorbent at the rate of 2 wt.% Of the daily diet.

The results are shown in table. 1. As can be seen from the table, 2 minutes after the administration of toxicants in groups of untreated animals, the percentage of echinocytes is up to 58-63 and remains at the same level (52-58%) during the 10-minute observation period; in groups of animals treated only with sorbents, the initial percentage of echinocytes is 50-56, and after 10 minutes of observation it decreases to 25-44%, and the smallest decrease in this indicator (up to 35-44%) is observed in case of poisoning caused by alcohol or drug ; in groups of animals treated with sorbents and blood pressure, the percentage of echinocytes after 2 minutes is 36-52, and after 10 minutes of observation is reduced to 18-42%. Of the enterosorbents of plant origin, the most effective were filter and zosterol. When used in combination with blood pressure, the percentage of echinocytes within 10 minutes decreases to the level of 18-32%. Recen RD showed slightly lower antitoxic activity from this group of enterosorbents (18-38% of echinocytes after 10 min). The effectiveness of known agents (polyphepam and activated carbon) even in combination with blood pressure is slightly lower (38-42% of echinocytes after 10 minutes).

EXAMPLE 2. The study of the dynamics of normalization of an alcoholized organism is carried out in three groups (25 individuals) of rats adapted to alcohol:

- receiving filtrum (2% of the daily diet daily for 10 days);

- receiving filtrum in the same dosage and blood pressure (daily for 10 days once for 30 minutes with a vacuum of -0.15 kg / cm ² );

- without treatment (control).

The results are shown in table.2. As can be seen from the table, in the group of animals adapted to chronic alcohol intake, during the observation period (31 days) the phagocytic activity of neutrophils is 29.9-32.1%; phagocytic index - 1.0-1.5; phagocytic intensity - 0.2-0.5; survival rate - 40%. In the group of animals treated with filter, over the period of observation from 10 to 31 days, the phagocytic activity of neutrophils increased from 36.4 to 68.4%; phagocytic index - from 3.6 to 5.9; phagocytic intensity - from 1.4 to 4.5; 92% of the animals survived. In the group of animals treated with filtrum and blood pressure, over the same period, the phagocytic activity of neutrophils increased from 39.8 to 75.2%; phagocytic index - from 3.9 to 6.7; phagocytic intensity - from 1.3 to 5.7; survival rate - 100%.

EXAMPLE 3. A group of four patients (age 53-61 years) with a diagnosis of addiction syndrome, alcohol withdrawal state (with in-depth in-patient examination revealed hepatopathy) after relief of acute withdrawal symptoms in the St. Petersburg City Narcological Hospital from 7-10 days of hospital stay, 10 daily BP cycles were carried out with a vacuum of -0.1 to -0.2 kg / cm ² for 45 minutes (three 15-minute sessions per day). On the eve of each BP cycle, patients took the Enterosorbent "Filterum" orally in a dosage according to the instructions for its use.

We evaluated the level of asthenia on the MF-20 scale. Before the start of blood pressure sessions, this level was 18 ± 2, and after the sessions decreased to 10 ± 1.

EXAMPLE 4. Rehabilitation therapy in a group of 8 drug addicts is carried out as in example 1 using zosterol as an enterosorbent. Before the start of the blood pressure sessions, the level of asthenia in the group was 17 ± 2, and at the end of treatment - 11 ± 2.

Due to the fact that of the 3 and 4 patients shown in examples, all 12 people showed a decrease in the level of asthenia; the confidence interval in relation to a positive result is established according to the student criterion in the interpretation of van der Waerden:

where P is the confidence interval,%;

n is the number of observations.

Consequently,

When testing the proposed method in the toxicological department of St. Petersburg Research Institute of Emergency Medicine. I.I.Dzhanelidze found that the local decompression procedure in combination with treatment with enterosorbents reduces the number of deformed red blood cells in peripheral blood in patients with toxicosis of different etiologies by 1.3–2.3 times (Table 3).

Summarizing the above examples, we can conclude that the use of the proposed combined effect of enterosorbents of plant origin and blood pressure increases the efficiency of normalizing the physiological state of the body in the treatment of toxicosis and expands the arsenal of agents (enterosorbents and blood pressure) used for this purpose.

Table 1 The percentage of echinocytes in rats in the dynamics of treatment of acute poisoning TOXICANT SORBENT MODE
HELL,
KG / CM ² The percentage of echinocytes at the end 2 minutes 5 minutes 10 minutes CARBOFOS Filterum -0.02
-0.15
- 47 ± 2
41 ± 3
50 ± 3 40 ± 3
37 ± 2
43 ± 4 22 ± 2
21 ± 3
25 ± 3 Zosterin -0.05
-0.20
- 47 ± 3
36 ± 5
52 ± 3 43 ± 3
34 ± 3
45 ± 4 24 ± 3
18 ± 4
28 ± 3 Rekitsen -0.10
-0.20 50 ± 4
48 ± 3 48 ± 3
46 ± 4 33 ± 2
30 ± 3 Polypepam -0.15 55 ± 6 50 ± 4 41 ± 3 Act. Coal -0.15 53 ± 3 47 ± 5 38 ± 2 - - 60 ± 3 55 ± 4 52 ± 4 Ethanol Feedroom -0.05
-0.20
- 47 ± 4
45 ± 5
56 ± 3 39 ± 4
40 ± 3
48 ± 3 27 ± 3
32 ± 3
42 ± 2 Zosterin -0.10
-0.20
- 46 ± 3
45 ± 3
55 ± 3 38 ± 4
40 ± 2
48 ± 2 21 ± 2
23 ± 1
38 ± 3 - - 63 ± 4 64 ± 3 58 ± 3 MORPHIN Zosterin -0.10
-0.20
- 48 ± 5
44 ± 4
54 ± 4 39 ± 3
37 ± 3
46 ± 3 30 ± 2
29 ± 2
35 ± 3 Rekitsen -0.10
-0.20
- 52 ± 3
48 ± 4
56 ± 4 45 ± 3
41 ± 3
49 ± 3 38 ± 3
34 ± 2
44 ± 4 Polypepam -0.15 53 ± 3 47 ± 3 42 ± 3 Act. coal -0.15 51 ± 2 44 ± 3 40 ± 2 - - 58 ± 3 54 ± 2 52 ± 3 THE CONTROL - - 18 ± 2 20 ± 1 20 ± 1

table 2 The effect of filtrum in combination with and without blood pressure on the immune system of alcoholized rats group of animals Phagocytic activity of neutrophils,% Phagocytic index Phagocytic intensity Survival,
% 10 days 31 days 10 days 14 days 31 days 10 days 14 days 31 days Received Filtrum 36,4 68,4 3.6 3.9 5.9 1.4 2.1 4,5 92 Received Filtrum + HELL 39.8 75,2 3.9 4,5 6.7 1.3 2,8 5.7 100 Alcohol adapted 26.9 32.1 1,0 0.8 1,5 0.2 0.2 0.5 40

Table 3 The ratio of deformed red blood cells before and after abdominal local decompression in patients with toxicosis of various etiologies Research No. To HELL After blood pressure normal red blood cells,% deformed red blood cells,% normal red blood cells,% deformed red blood cells,% 1 56 ± 1,0 44 ± 0.3 64 ± 1,3 36 ± 0.1 2 52 ± 0.1 48 ± 0.1 65 ± 0.9 35 ± 0.2 3 51 ± 0.1 49 ± 0.2 77 ± 1,1 23 ± 0.3 4 70 ± 1.3 30 ± 0.1 88 ± 1.3 13 ± 0.1 5 54 ± 0.9 47 ± 0.7 65 ± 0.7 36 ± 1,0

Claims (2)

1. A method of normalizing the physiological state of an organism in the treatment of toxicosis by detoxification therapy by oral administration of an enterosorbent of plant origin in combination with a physiotherapeutic effect, characterized in that abdominal decompression is performed as a physiotherapeutic effect. 2. The method according to claim 1, characterized in that as an enterosorbent use filtrum or zosterin.