RU2008895C1 - Method of producing biologically active extract for confectionery articles - Google Patents

Abstract

FIELD: chemical and pharmaceutical industry. SUBSTANCE: extract is prepared of silkworm cocoon by extraction with ethyl alcohol at ratio 0.6: 1-0.1: 1.4. The process is carried out within 1.5-2 h at 40-60 C, it is followed by desired product separation and by distillation extragent of raw materials residue. The latter process takes place with the help of neutral gas at 70-80 C. EFFECT: increases extractive yield, improves the process. 1 tbl

Description

 The invention relates to the pharmaceutical industry and relates to the production of a biologically active extract for cosmetic products, in particular to the production of extracts from natural sources.

 Known methods for producing biologically active substances from natural sources, involving the extraction of the target product from the starting material, which, if necessary, can be previously crushed. In the future, the target product is usually subjected to further purification using known techniques. Thus, a known method of producing biologically active additives for the cosmetic industry by fine grinding the purified silk obtained after drawing from a cocoon or silk yarn.

 Grinding is carried out with the curing effect of low temperatures, followed by dissolution of the powder in alcohol.

 There is also known a method of obtaining a complex of biologically active substances from animal raw materials, which consists in the fact that the tissue is extracted with an organic solvent and suspended particles are separated.

A method of obtaining biologically active substances from a silkworm pupa, consists in the fact that the live pupae extracted from cocoon, maintained at 0-4 ^{° C} for four days, compressed at low temperature, the resulting oil was collected, added water and packaged.

As the closest technical solution, the method of obtaining a complex of biologically active substances by grinding 100-500 g of pupae and butterflies of mulberry and oak silkworms with 40-96% alcohol, incubation with stirring for 40 minutes - 180 hours at a temperature of 3-75 ^o C filtering, followed by separation of the extract containing the target product, centrifugation at 7000 rpm for 10 minutes

However, the known methods for the preparation of biologically active substances from products of silk production do not allow to obtain a high yield of biologically active substances, to achieve waste-free technology with a relatively shortened process time. The proposed method is that the dolly is ground and extracted with ethyl alcohol at a ratio of feedstock-extractant (0.6: 1) - (0.6: 1.4) at a temperature of extractant 40-60 ^{° C} for 1.5-2 h followed by distillation of the extractant and drying with neutral gas at 70-80 ^about C.

 The extraction is carried out three times.

 An example of the preparation of the extract by the proposed method.

 55-57 kg of crushed silkworm pupae are loaded into the extraction chamber in cloth bags and 100-140 liters of ethanol are poured, the extractor is sealed, the coolant is supplied to the evaporator pipes and the refrigerant to the condenser pipes. The extract is poured into an evaporator.

When the extract boils, alcohol vapors enter the condenser and, condensing, form an alcohol condensate, which enters the extraction chamber. After filling the chamber, the extract is poured into the evaporator. The process takes 25-30 minutes. The specified operation is carried out three times, transfusing the extract into the evaporator. Then, the extraction chamber is fed neutral gas nitrogen at 70-80 ^{° C} for stripping the extractant from the pupa and drying it. The process takes 40 minutes. After drying, it is discharged from the extraction chamber and a new portion of the pupa is loaded. When refueling 100 liters of alcohol, 60 liters of extract are obtained. When refueling 140 l - 100 l of extract. Production waste can be used as feed for farm animals.

 The advantage of the proposed method for producing biologically active extracts from silkworm pupae consists in a high yield of extractive substances, simplification of the technological process, 100% use of an organic solvent, achieving standardization of the finished product due to the constancy of the module, low energy consumption, environmentally friendly production.

 The biologically active extract from the silkworm pupa obtained by the proposed method is named "Victoria".

 The alcohol extract of the Victoria silkworm pupa was tested experimentally at the Moscow Institute of Beauty’s cosmetology clinic.

 In an animal experiment, it was found that a sample of Victoria extract containing 400 mg / ml dry matter belongs to the V class of toxicity according to the classification of K. K. Sidorov, DL 50 is equal to 1.88 g / kg, DL 100 is 3.6 g / kg weight of white outbred male mice with an intraperitoneal route of administration.

 Liposomal Thomas extract of "Victoria" extract containing 20 mg / l of extractive substances when administered to mice intraperitoneally at a maximum dose of 25 g / kg with a content of extractive substances of 500 mg / kg did not cause animal death. DL 50 liposomal form above 500 mg / kg animal weight.

 A morphological study of biopsy samples of the skin of the liver of mice showed no pathological changes in their structure.

 Water-oil emulsions of Victoria extract of liposomal form of Victoria extract in 0.05%; 0.1% and 0.5% concentrations did not cause an irritating allergenic effect during prolonged skin application.

 The morphological study of biopsies of the skin and liver did not cause pathological changes in the structure of the skin and liver of rats.

 Biochemical studies of rat skin showed that water-in-oil emulsions of Victoria extract in 0.05% and 0.1% concentration did not change the level of total proteins and lipids of rat skin. At 0.5% concentration, a decrease in total lipids of 13.8% and an increase in the content of soluble proteins by 26.6% were observed.

 The liposomal form of the Victoria extract is more pronounced.

 Water-oil emulsion of Victoria extract and liposome firms of Victoria extract at 0.05; 0.1 and 0.5% of the concentration did not cause an irritating allergic effect during their prolonged skin use.

 Water-oil emulsions of the liposome form of the Victoria extract in 0.05% concentration do not change the level of total lipids and increase the content of total proteins by 8; 0.1 and 0.5% concentration of the drug stimulated the content of total proteins by 15 and 27% compared with the control. (56) French Patent N 2517964, cl. A 61 K 7/00, 1985.

Claims (1)

METHOD FOR PRODUCING BIOLOGICALLY ACTIVE EXTRACT "VICTORIA" FOR COSMETIC PRODUCTS by extraction of a silkworm pupa with ethyl alcohol followed by separation of the target product, characterized in that the extraction is carried out at a ratio of raw material-extractant 0.6: 1 - 0.6: 1.4 for 1 , 5 - 2 hours and a temperature of 40 - 60 ^o C, while the process is repeated three times, and after separation of the extract, the extractant from the residual raw materials is removed with neutral gas at a temperature of 70 - 80 ^o C.

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