RU2001621C1

RU2001621C1 - Method of inulin preparation

Info

Publication number: RU2001621C1
Authority: RU
Inventors: Виктор Гаврилович Борисенко; Валентин Дмитриевич Королев; Филипп Романович Педанов; Виктор Иванович Кондратенко; Николай Матвеевич Пасько
Original assignee: Borisenko Viktor G; Korolev Valentin D; Pedanov Filipp R; Kondratenko Viktor I; Pasko Nikolaj M
Priority date: 1992-05-29
Filing date: 1992-05-29
Publication date: 1993-10-30

Description

вращение от -32 до -40°, содержание кальци не более 0,1%, рН 5,0-7,0, хлорида не более 0,015%, сульфаты отсутствуют, железо отсутствует, т желые металлы отсутствуют , радиоактивные Cs и Cs в пределах фона, свободна фруктоза не более 2%.rotation from -32 to -40 °, calcium content not more than 0.1%, pH 5.0-7.0, chloride not more than 0.015%, sulfates absent, iron absent, heavy metals absent, radioactive Cs and Cs within background, free fructose is not more than 2%.

Хроматографический анализ показал наличие на хроматограмме одного типа, аналогичного эталону.Chromatographic analysis showed the presence on the chromatogram of one type, similar to the standard.

Химическа формула следующа :The chemical formula is as follows:

н-с н-с-онns ss he

сн-онsn-he

п - 12-28n - 12-28

Выбор указанных параметров режима вл етс оптимальным при использовании 25% спирта с максимальным выходом инулина . Повышение концентрации приводит к загр знению продукта примес ми пектина.The selection of these mode parameters is optimal when using 25% alcohol with a maximum inulin yield. An increase in concentration leads to contamination of the product with pectin impurities.

Соотношение 1:1 позвол ет с достаточной степенью эффективности максимально проэкстрагировать продукт при температуФормула изобретени A ratio of 1: 1 allows, with a sufficient degree of efficiency, to maximize the extraction of the product at a temperature.

Claims

1. METHOD FOR PRODUCING INULIN by extracting crushed tubers of Jerusalem artichoke Hellantus tuberesus, separating the extract and purifying, characterized in that, in order to increase the degree of purification, the tubers are crushed simultaneously with the extractant, 25% aqueous solution is used as the extractant ethanol solution

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re 1-4 ° C. A chip size of less than 0.3 mm ensures the preservation of the cell, which also makes it possible to selectively extract with preservation of the inulin nativeness.

Thus, the developed method for the production of inulin from Tiponambur tubers allows one to obtain highly purified inulin that meets the requirements for impurity content for medical preparations. In addition, the developed technology is technologically advanced, which allows for scaling of the process and for carrying out the process under production conditions. The method is also mild, sparing, which does not lead to hydrolysis of inulin, determined by the content of free fructose (not more than 2%). which indicates the preservation of the native structure of the target product. In addition, a high yield of the expected product of 10% is maintained. i.e., 10 kg of 100 kg of crushed raw materials, taking into account the inulin content by weight of solids in the tubers of Jerusalem artichoke, 12.6-22%. This is a rather high yield, which allows us to consider the technology highly economical.

(56) Appl. Blochem. Blotechnol, 1988, v. 17, p. 45. 0

Extraction is carried out at a feed: solvent ratio of 1: 1 at 1-4 ° C for at least 10 days, and the extract is purified by ultrasonography on UAM-50 membranes and ion-exchange chromatography successively on KU-2 cation exchange resin and AB-17 anion exchange resin and dried.

2. The method according to claim 1, characterized in that

0 that, in order to optimize the process, the tubers are chopped to a chip size of less than 0.3 mm.

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