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PT3290528T - Métodos e composições para sequenciamento de ácido nucleico - Google Patents

Métodos e composições para sequenciamento de ácido nucleico

Info

Publication number
PT3290528T
PT3290528T PT171805831T PT17180583T PT3290528T PT 3290528 T PT3290528 T PT 3290528T PT 171805831 T PT171805831 T PT 171805831T PT 17180583 T PT17180583 T PT 17180583T PT 3290528 T PT3290528 T PT 3290528T
Authority
PT
Portugal
Prior art keywords
compositions
methods
nucleic acid
acid sequencing
sequencing
Prior art date
Application number
PT171805831T
Other languages
English (en)
Inventor
Mark Boutell Jonathan
A Eltoukhy Helmy
Feng Wenyi
Hirschbein Bernard
Joseph Thomas
C Kain Robert
Klausing Kay
Liu Xiaohai
Richard Shen Min-Jui
Paul Smith Geoffrey
Smith Randall
Tregidgo Carolyn
Wu Xiaolin
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=46964103&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=PT3290528(T) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of PT3290528T publication Critical patent/PT3290528T/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
PT171805831T 2011-09-23 2012-09-21 Métodos e composições para sequenciamento de ácido nucleico PT3290528T (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161538294P 2011-09-23 2011-09-23
US201261619878P 2012-04-03 2012-04-03

Publications (1)

Publication Number Publication Date
PT3290528T true PT3290528T (pt) 2019-10-14

Family

ID=46964103

Family Applications (2)

Application Number Title Priority Date Filing Date
PT171805831T PT3290528T (pt) 2011-09-23 2012-09-21 Métodos e composições para sequenciamento de ácido nucleico
PT191899277T PT3623481T (pt) 2011-09-23 2012-09-21 Composições para sequenciação de ácidos nucleicos

Family Applications After (1)

Application Number Title Priority Date Filing Date
PT191899277T PT3623481T (pt) 2011-09-23 2012-09-21 Composições para sequenciação de ácidos nucleicos

Country Status (15)

Country Link
US (5) US9453258B2 (pt)
EP (4) EP3290528B1 (pt)
CA (2) CA2859660C (pt)
CY (1) CY1125261T1 (pt)
DK (3) DK3290528T3 (pt)
ES (3) ES2639938T5 (pt)
HR (1) HRP20211523T1 (pt)
HU (1) HUE056246T2 (pt)
LT (1) LT3623481T (pt)
PL (2) PL3623481T3 (pt)
PT (2) PT3290528T (pt)
RS (1) RS62530B1 (pt)
SI (1) SI3623481T1 (pt)
SM (1) SMT202100624T1 (pt)
WO (1) WO2013044018A1 (pt)

Families Citing this family (351)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
WO2007135368A2 (en) 2006-05-18 2007-11-29 Solexa Limited Dye compounds and the use of their labelled conjugates
US11940413B2 (en) 2007-02-05 2024-03-26 IsoPlexis Corporation Methods and devices for sequencing nucleic acids in smaller batches
US9163053B2 (en) * 2007-05-18 2015-10-20 Fluidigm Corporation Nucleotide analogs
US9017973B2 (en) 2008-03-19 2015-04-28 Intelligent Biosystems, Inc. Methods and compositions for incorporating nucleotides
US9371598B2 (en) 2010-04-05 2016-06-21 Prognosys Biosciences, Inc. Spatially encoded biological assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US9387476B2 (en) 2010-10-27 2016-07-12 Illumina, Inc. Flow cells for biological or chemical analysis
EP2632593B1 (en) 2010-10-27 2021-09-29 Illumina, Inc. Flow cells for biological or chemical analysis
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
CN103748236B (zh) 2011-04-15 2018-12-25 约翰·霍普金斯大学 安全测序系统
PL3623481T3 (pl) 2011-09-23 2022-01-17 Illumina, Inc. Kompozycje do sekwencjonowania kwasów nukleinowych
US9062091B2 (en) * 2012-02-15 2015-06-23 Pacific Biosciences Of California, Inc. Polymerase enzyme substrates with protein shield
EP3511423B2 (en) 2012-10-17 2024-05-29 Spatial Transcriptomics AB Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
PL2912468T3 (pl) 2012-10-29 2019-04-30 Univ Johns Hopkins Test papanicolaou pod kątem raka jajnika i endometrium
CA2903095C (en) 2013-03-15 2018-02-13 Xiaohai Liu Modified nucleosides or nucleotides
LT3013983T (lt) 2013-06-25 2023-05-10 Prognosys Biosciences, Inc. Erdviniai koduoti biologiniai tyrimai, naudojant mikrofluidinį įrenginį
ES2719579T3 (es) 2013-07-03 2019-07-11 Illumina Inc Sistema para secuenciación por síntesis ortogonal
MX370560B (es) 2013-08-08 2019-12-17 Illumina Inc Sistema de fluidos para distribución de reactivo a una celda de flujo.
PT3077943T (pt) 2013-12-03 2020-08-21 Illumina Inc Métodos e sistemas para analisar dados de imagem
MX379233B (es) 2014-02-18 2025-03-10 Illumina Inc Métodos y composiciones para el perfilado de adn.
US10767219B2 (en) 2014-03-11 2020-09-08 Illumina, Inc. Disposable, integrated microfluidic cartridge and methods of making and using same
SG11201610168YA (en) * 2014-05-16 2017-01-27 Illumina Inc Nucleic acid synthesis techniques
AU2015267189B2 (en) 2014-05-27 2019-12-05 Illumina, Inc. Systems and methods for biochemical analysis including a base instrument and a removable cartridge
US20150353989A1 (en) 2014-06-09 2015-12-10 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
GB201414098D0 (en) 2014-08-08 2014-09-24 Illumina Cambridge Ltd Modified nucleotide linkers
WO2016040602A1 (en) 2014-09-11 2016-03-17 Epicentre Technologies Corporation Reduced representation bisulfite sequencing using uracil n-glycosylase (ung) and endonuclease iv
EP3191606B1 (en) 2014-09-12 2020-05-27 Illumina, Inc. Methods for detecting the presence of polymer subunits using chemiluminescence
US9897791B2 (en) 2014-10-16 2018-02-20 Illumina, Inc. Optical scanning systems for in situ genetic analysis
GB201418718D0 (en) * 2014-10-21 2014-12-03 Cambridge Epigenetix Ltd Improved nucleic acid re-sequencing using a reduced number of identified bases
US10190162B2 (en) 2014-10-23 2019-01-29 Complete Genomics, Inc. Signal confinement sequencing (SCS) and nucleotide analogues for signal confinement sequencing
GB201419731D0 (en) 2014-11-05 2014-12-17 Illumina Cambridge Ltd Sequencing from multiple primers to increase data rate and density
EP3215616B1 (en) 2014-11-05 2019-12-18 Illumina Cambridge Limited Reducing dna damage during sample preparation and sequencing using siderophore chelators
CN107208019B (zh) 2014-11-11 2021-01-01 伊鲁米纳剑桥有限公司 用于核酸单克隆簇的产生和测序的方法和阵列
US20160160169A1 (en) 2014-12-03 2016-06-09 IsoPlexis Corporation Analysis and screening of poly-functional secretion profiles
EP3271073B1 (en) 2015-03-20 2019-06-12 Illumina, Inc. Fluidics cartridge for use in the vertical or substantially vertical position
EP3277839B1 (en) 2015-03-31 2020-12-02 Illumina Cambridge Limited Surface concatamerization of templates
SG11201707515SA (en) 2015-04-10 2017-10-30 Spatial Transcriptomics Ab Spatially distinguished, multiplex nucleic acid analysis of biological specimens
WO2016183218A1 (en) 2015-05-12 2016-11-17 Illumina, Inc. Field-effect apparatus and methods for sequencing nucelic acids
WO2016196358A1 (en) 2015-05-29 2016-12-08 Epicentre Technologies Corporation Methods of analyzing nucleic acids
EP3303614B1 (en) 2015-05-29 2020-03-11 Illumina Cambridge Limited Enhanced utilization of surface primers in clusters
CN107922966B (zh) 2015-07-06 2022-06-28 伊卢米纳剑桥有限公司 用于核酸扩增的样品制备
ES2888626T3 (es) 2015-07-27 2022-01-05 Illumina Inc Cartografiado espacial de información de secuencia de ácidos nucleicos
WO2017019278A1 (en) 2015-07-30 2017-02-02 Illumina, Inc. Orthogonal deblocking of nucleotides
US11286531B2 (en) 2015-08-11 2022-03-29 The Johns Hopkins University Assaying ovarian cyst fluid
IL295355B2 (en) 2015-08-14 2023-11-01 Illumina Inc Systems And Methods Using Magnetically-Responsive Sensors For Determining A Genetic Characteristic
CN105112516A (zh) * 2015-08-14 2015-12-02 深圳市瀚海基因生物科技有限公司 一种单分子靶向测序方法、装置、系统及应用
CN116338219A (zh) 2015-08-24 2023-06-27 亿明达股份有限公司 用于生物和化学测定的线路内蓄压器和流量控制系统
WO2017058953A1 (en) 2015-09-28 2017-04-06 The Trustees Of Columbia University In The City Of New York Design and synthesis of novel disulfide linker based nucleotides as reversible terminators for dna sequencing by synthesis
JP2018534317A (ja) * 2015-11-18 2018-11-22 シナジー ファーマシューティカルズ インコーポレイテッド 結腸がんの処置および検出のための組成物および方法
CN108474744B (zh) 2016-03-24 2019-11-05 伊鲁米那股份有限公司 在发光成像中使用的基于光子超晶格的设备和组成物及其使用方法
IL262447B2 (en) 2016-04-22 2023-09-01 Illumina Inc Photonic structure-based devices and compositions for use in luminescent imaging of sites in a pixel and methods of using the devices and compositions
US10988501B2 (en) 2016-04-22 2021-04-27 Mgi Tech Co., Ltd. Reversibly blocked nucleoside analogues and their use
CN116656795A (zh) 2016-05-23 2023-08-29 纽约哥伦比亚大学董事会 核苷酸衍生物及其使用方法
AU2017299803B2 (en) 2016-07-22 2023-06-29 Illumina, Inc. Single cell whole genome libraries and combinatorial indexing methods of making thereof
JP6828140B2 (ja) 2016-08-15 2021-02-10 オムニオム インコーポレイテッドOmniome, Inc. 核酸をシーケンシングするための方法及びシステム
WO2018049418A1 (en) 2016-09-12 2018-03-15 IsoPlexis Corporation System and methods for multiplexed analysis of cellular and other immunotherapeutics
US10385214B2 (en) 2016-09-30 2019-08-20 Illumina Cambridge Limited Fluorescent dyes and their uses as biomarkers
JP7348066B2 (ja) 2016-11-11 2023-09-20 アイソプレキシス コーポレイション 単一細胞のゲノム、トランスクリプトームおよびプロテオームの同時解析のための組成物および方法
US11525783B2 (en) 2016-11-22 2022-12-13 IsoPlexis Corporation Systems, devices and methods for cell capture and methods of manufacture thereof
WO2018114710A1 (en) 2016-12-22 2018-06-28 Illumina Cambridge Limited Coumarin compounds and their uses as fluorescent labels
RU2760737C2 (ru) * 2016-12-27 2021-11-30 Еги Тек (Шэнь Чжэнь) Ко., Лимитед Способ секвенирования на основе одного флуоресцентного красителя
HUE071446T2 (hu) 2017-01-04 2025-09-28 Mgi Tech Co Ltd Lépésenkénti szekvenálás nem jelölt reverzibilis terminátorokkal vagy természetes nukleotidokkal
EP3566158B1 (en) 2017-01-06 2022-04-20 Illumina, Inc. Phasing correction
US20200090784A1 (en) 2017-01-17 2020-03-19 Illumina, Inc. Oncogenic splice variant determination
GB201701686D0 (en) 2017-02-01 2017-03-15 Illunina Inc System & method with fiducials having offset layouts
WO2018144531A1 (en) 2017-02-01 2018-08-09 Illumina, Inc. System and method with fiducials responding to multiple excitation frequencies
GB201701689D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials of non-closed shapes
GB201701688D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials in non-recliner layouts
US12018325B2 (en) 2017-03-28 2024-06-25 The Trustees Of Columbia University In The City Of New York 3′-O-modified nucleotide analogues with different cleavable linkers for attaching fluorescent labels to the base for DNA sequencing by synthesis
SG11201909914RA (en) 2017-04-23 2019-11-28 Illumina Cambridge Ltd Compositions and methods for improving sample identification in indexed nucleic acid libraries
US9951385B1 (en) * 2017-04-25 2018-04-24 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
RU2770879C2 (ru) 2017-06-07 2022-04-22 Орегон Хэлт Энд Сайенс Юниверсити Полногеномные библиотеки отдельных клеток для бисульфитного секвенирования
GB2578038B (en) * 2017-06-16 2022-11-23 Life Technologies Corp Control nucleic acids, and compositions, kits, and uses thereof
EP3642362B1 (en) 2017-06-20 2025-10-15 Illumina, Inc. Methods for addressing inefficiencies in amplification reactions
CN109207571B (zh) * 2017-06-30 2022-01-04 深圳华大生命科学研究院 一种检测核酸内切酶酶切位点的方法
WO2019027767A1 (en) 2017-07-31 2019-02-07 Illumina Inc. SEQUENCING SYSTEM COMPRISING AGGREGATION OF MULTIPLEXED BIOLOGICAL SAMPLES
FI3662083T3 (fi) 2017-08-01 2024-11-20 Illumina Inc Geneettisen materiaalin ja kirjastovalmisteen spatiaalinen indeksointi hydrogeelihelmiä ja virtauskennoja käyttämällä
MX2020001575A (es) 2017-08-07 2020-11-18 Univ Johns Hopkins Materiales y métodos para evaluar y tratar el cáncer.
KR102500210B1 (ko) * 2017-11-06 2023-02-15 일루미나, 인코포레이티드 핵산 색인 기술
NL2020636B1 (en) * 2017-12-28 2019-07-08 Illumina Inc Light energy fluorescence excitation
CA3065934A1 (en) 2018-01-08 2019-07-11 Illumina, Inc. High-throughput sequencing with semiconductor-based detection
US11378544B2 (en) 2018-01-08 2022-07-05 Illumina, Inc. High-throughput sequencing with semiconductor-based detection
WO2019161253A1 (en) 2018-02-16 2019-08-22 Ultima Genomics, Inc. Methods for sequencing with single frequency detection
CN112218640A (zh) 2018-03-15 2021-01-12 哥伦比亚大学董事会 核苷酸类似物及其在核酸测序和分析中的用途
JP6974504B2 (ja) 2018-04-02 2021-12-01 イルミナ インコーポレイテッド 配列に基づく遺伝子試験のための対照を作製するための組成物及び方法
CA3097583A1 (en) 2018-04-19 2019-10-24 Omniome, Inc. Improving accuracy of base calls in nucleic acid sequencing methods
IL271454B2 (en) 2018-05-17 2025-04-01 Illumina Inc Rapid single-cell genetic sequencing with low Aggregation bias
US11180794B2 (en) 2018-05-31 2021-11-23 Omniome, Inc. Methods and compositions for capping nucleic acids
WO2019231568A1 (en) 2018-05-31 2019-12-05 Omniome, Inc. Increased signal to noise in nucleic acid sequencing
US20210102194A1 (en) 2018-06-04 2021-04-08 Illumina, Inc. High-throughput single-cell transcriptome libraries and methods of making and of using
NL2021258B1 (en) 2018-06-14 2019-12-20 Illumina Inc Device for luminescent imaging
CN113366117B (zh) 2018-08-28 2025-08-05 10X基因组学股份有限公司 用于生物样品中转座酶介导的空间标记和分析基因组dna的方法
WO2020047005A1 (en) 2018-08-28 2020-03-05 10X Genomics, Inc. Resolving spatial arrays
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
KR102749871B1 (ko) 2018-11-07 2025-01-07 칭다오 엠쥐아이 테크 컴퍼니 리미티드 폴리뉴클레오티드의 시퀀싱 방법
AU2019391274B2 (en) 2018-12-05 2025-10-30 Illumina Cambridge Limited Methods and compositions for cluster generation by bridge amplification
WO2020123316A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Methods for determining a location of a biological analyte in a biological sample
ES2973302T3 (es) 2018-12-14 2024-06-19 Illumina Cambridge Ltd Disminución del ajuste de fase con nucleótidos sin marcar durante la secuenciación
ES3032918T3 (en) 2018-12-17 2025-07-28 Illumina Cambridge Ltd Method of polynucleotide sequencing
EP3899041A1 (en) 2018-12-18 2021-10-27 Illumina Cambridge Limited Methods and compositions for paired end sequencing using a single surface primer
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2020149982A1 (en) 2019-01-14 2020-07-23 Illumina, Inc. Flow cell including a heteropolymer
EP3702474A1 (en) * 2019-02-26 2020-09-02 QIAGEN GmbH Sequencing method and kit
CN114174531A (zh) 2019-02-28 2022-03-11 10X基因组学有限公司 用空间条码化寡核苷酸阵列对生物分析物进行概况分析
WO2020178231A1 (en) 2019-03-01 2020-09-10 Illumina, Inc. Multiplexed fluorescent detection of analytes
MX2021003746A (es) 2019-03-01 2021-06-23 Illumina Inc Genotecas de núcleos únicos y células únicas de alto rendimiento y métodos para elaborarlas y usarlas.
NL2023327B1 (en) * 2019-03-01 2020-09-17 Illumina Inc Multiplexed fluorescent detection of analytes
JP7587424B2 (ja) 2019-03-01 2024-11-20 イルミナ ケンブリッジ リミテッド 環外アミン置換クマリン化合物およびそれらの蛍光標識としての使用
CN114127309A (zh) 2019-03-15 2022-03-01 10X基因组学有限公司 使用空间阵列进行单细胞测序的方法
US11210554B2 (en) 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
US11676685B2 (en) 2019-03-21 2023-06-13 Illumina, Inc. Artificial intelligence-based quality scoring
EP3887542A1 (en) 2019-03-22 2021-10-06 10X Genomics, Inc. Three-dimensional spatial analysis
US11421271B2 (en) 2019-03-28 2022-08-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing using photoswitchable labels
WO2020210370A1 (en) 2019-04-12 2020-10-15 Roche Sequencing Solutions, Inc. Nucleic acid sequencing by synthesis using magnetic sensor arrays
US11609208B2 (en) 2019-04-12 2023-03-21 Western Digital Technologies, Inc. Devices and methods for molecule detection based on thermal stabilities of magnetic nanoparticles
US11738336B2 (en) 2019-04-12 2023-08-29 Western Digital Technologies, Inc. Spin torque oscillator (STO) sensors used in nucleic acid sequencing arrays and detection schemes for nucleic acid sequencing
US11112468B2 (en) 2019-04-12 2021-09-07 Western Digital Technologies, Inc. Magnetoresistive sensor array for molecule detection and related detection schemes
US11327073B2 (en) 2019-04-12 2022-05-10 Western Digital Technologies, Inc. Thermal sensor array for molecule detection and related detection schemes
US11579217B2 (en) 2019-04-12 2023-02-14 Western Digital Technologies, Inc. Devices and methods for frequency- and phase-based detection of magnetically-labeled molecules using spin torque oscillator (STO) sensors
CA3139169A1 (en) * 2019-05-15 2020-11-19 Egi Tech (Shen Zhen) Co., Limited Single-channel sequencing method based on self-luminescence
US11423306B2 (en) 2019-05-16 2022-08-23 Illumina, Inc. Systems and devices for characterization and performance analysis of pixel-based sequencing
AU2020273459A1 (en) 2019-05-16 2021-01-07 Illumina, Inc. Base calling using convolutions
US11593649B2 (en) 2019-05-16 2023-02-28 Illumina, Inc. Base calling using convolutions
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
US11282588B2 (en) 2019-05-31 2022-03-22 Illumina, Inc. Storage device, system, and method
CN112689751A (zh) 2019-05-31 2021-04-20 伊鲁米那股份有限公司 具有一个或更多个屏障特征的流通池
EP3977455A4 (en) 2019-05-31 2023-07-12 Illumina, Inc. STORAGE SYSTEM AND METHOD
WO2020243073A1 (en) 2019-05-31 2020-12-03 Illumina, Inc. Systems and methods for information storage and retrieval using flow cells
US11137385B2 (en) 2019-05-31 2021-10-05 Illumina, Inc. Obtaining information from a biological sample in a flow cell
CN112673251A (zh) 2019-05-31 2021-04-16 伊鲁米那股份有限公司 核苷酸选择性沉积或激活的流通池
EP3997101A4 (en) * 2019-07-09 2024-03-13 The Trustees Of Columbia University In The City Of New York NOVEL NUCLEOTIDE ANALOGUES AND METHODS OF USE THEREOF
JP7523526B2 (ja) * 2019-08-20 2024-07-26 チンタオ エムジーアイ テック カンパニー リミテッド 発光標識及び二次発光信号の光信号速度論に基づくポリヌクレオチドの配列決定方法
US11208682B2 (en) 2019-09-13 2021-12-28 Western Digital Technologies, Inc. Enhanced optical detection for nucleic acid sequencing using thermally-dependent fluorophore tags
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
CN115210760B (zh) 2019-11-21 2023-08-01 10X基因组学有限公司 分析物的空间分析
US11747329B2 (en) 2019-11-22 2023-09-05 Western Digital Technologies, Inc. Magnetic gradient concentrator/reluctance detector for molecule detection
KR20220118295A (ko) 2019-12-19 2022-08-25 일루미나, 인코포레이티드 고 처리량 단일 세포 라이브러리, 및 이의 제조 방법 및 사용 방법
CN115038794A (zh) 2019-12-23 2022-09-09 10X基因组学有限公司 在基于分区的测定中使用固定生物样品的组合物和方法
CN121022991A (zh) 2019-12-23 2025-11-28 10X基因组学有限公司 使用rna模板化连接进行空间分析的方法
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US12112833B2 (en) 2020-02-04 2024-10-08 10X Genomics, Inc. Systems and methods for index hopping filtering
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US20220073977A1 (en) 2020-02-14 2022-03-10 The Johns Hopkins University Methods and materials for assessing nucleic acids
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
CN115485391A (zh) 2020-02-17 2022-12-16 10X基因组学有限公司 染色质相互作用的原位分析
IL295560A (en) 2020-02-20 2022-10-01 Illumina Inc Artificial intelligence-based many-to-many base calling
US20210265016A1 (en) 2020-02-20 2021-08-26 Illumina, Inc. Data Compression for Artificial Intelligence-Based Base Calling
US12354008B2 (en) 2020-02-20 2025-07-08 Illumina, Inc. Knowledge distillation and gradient pruning-based compression of artificial intelligence-based base caller
EP4107285B1 (en) 2020-02-21 2024-10-09 10X Genomics, Inc. Methods and compositions for integrated in situ spatial assay
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US12188085B2 (en) 2020-03-05 2025-01-07 10X Genomics, Inc. Three-dimensional spatial transcriptomics with sequencing readout
GB2608318A (en) * 2020-03-13 2022-12-28 Apton Biosystems Inc Densely-packed analyte layers and detection methods
EP4121559A4 (en) * 2020-03-18 2024-03-27 Pacific Biosciences of California, Inc. SYSTEMS AND METHODS FOR DETECTING DENSE ANALYTES
US20240002928A1 (en) * 2020-04-21 2024-01-04 Roche Sequencing Solutions, Inc. High-throughput nucleic acid sequencing with single-molecule-sensor arrays
ES2965354T3 (es) 2020-04-22 2024-04-12 10X Genomics Inc Métodos para análisis espacial que usan eliminación de ARN elegido como diana
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
CA3177286A1 (en) 2020-05-12 2021-11-18 Illumina Inc. Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
ES2989052T3 (es) 2020-05-22 2024-11-25 10X Genomics Inc Medición espacio-temporal simultánea de la expresión génica y la actividad celular
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
ES2981265T3 (es) 2020-06-08 2024-10-08 10X Genomics Inc Métodos para determinar un margen quirúrgico y métodos de uso del mismo
WO2021252617A1 (en) 2020-06-09 2021-12-16 Illumina, Inc. Methods for increasing yield of sequencing libraries
WO2021252591A1 (en) 2020-06-10 2021-12-16 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
JP2023531009A (ja) 2020-06-22 2023-07-20 イルミナ ケンブリッジ リミテッド 3’アセタールブロッキング基を有するヌクレオシド及びヌクレオチド
EP4172362B1 (en) 2020-06-25 2024-09-18 10X Genomics, Inc. Spatial analysis of dna methylation
WO2022006081A1 (en) 2020-06-30 2022-01-06 Illumina, Inc. Catalytically controlled sequencing by synthesis to produce scarless dna
US12168801B1 (en) 2020-07-02 2024-12-17 10X Genomics, Inc. Hybrid/capture probe designs for full-length cDNA
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
CN116018412A (zh) 2020-07-08 2023-04-25 Illumina公司 作为转座体载体的小珠
US11981964B2 (en) 2020-07-28 2024-05-14 Illumina Cambridge Limited Substituted coumarin dyes and uses as fluorescent labels
US11434525B2 (en) 2020-08-06 2022-09-06 Singular Genomics Systems, Inc. Spatial sequencing
US11492662B2 (en) 2020-08-06 2022-11-08 Singular Genomics Systems, Inc. Methods for in situ transcriptomics and proteomics
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
EP4200444A4 (en) 2020-10-30 2024-09-25 Singular Genomics Systems, Inc. METHODS AND COMPOSITIONS FOR REDUCING NUCLEOTIDE IMPURITIES CONTENT
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4012046A1 (en) 2020-12-11 2022-06-15 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
US20220195516A1 (en) * 2020-12-17 2022-06-23 Illumina Cambridge Limited Methods, systems and compositions for nucleic acid sequencing
GB2602063A (en) * 2020-12-17 2022-06-22 Sumitomo Chemical Co Sequencing method
EP4121555A1 (en) 2020-12-21 2023-01-25 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US20220195518A1 (en) * 2020-12-22 2022-06-23 Illumina Cambridge Limited Methods and compositions for nucleic acid sequencing
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
US20220235403A1 (en) 2021-01-26 2022-07-28 10X Genomics, Inc. Nucleic acid analog probes for in situ analysis
US20220282319A1 (en) 2021-03-03 2022-09-08 10X Genomics, Inc. Analyte detection in situ using nucleic acid origami
WO2022197752A1 (en) 2021-03-16 2022-09-22 Illumina, Inc. Tile location and/or cycle based weight set selection for base calling
JP7719206B2 (ja) 2021-04-02 2025-08-05 イルミナ インコーポレイテッド 配列決定のためのヌクレオチド-試料スライド内の泡を検出するための機械学習モデル
EP4428246B1 (en) 2021-04-14 2025-12-24 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
US12217829B2 (en) 2021-04-15 2025-02-04 Illumina, Inc. Artificial intelligence-based analysis of protein three-dimensional (3D) structures
US20220336057A1 (en) 2021-04-15 2022-10-20 Illumina, Inc. Efficient voxelization for deep learning
EP4334706A4 (en) 2021-05-05 2025-08-06 Singular Genomics Systems Inc MULTIOMICS ANALYSIS DEVICE AND METHODS OF USE THEREOF
WO2022236054A1 (en) 2021-05-06 2022-11-10 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
US20220396832A1 (en) 2021-05-20 2022-12-15 Illumina Cambridge Limited Compositions and methods for sequencing by synthesis
CN117396613A (zh) 2021-06-01 2024-01-12 10X基因组学有限公司 用于分析物检测和探针解析的方法和组合物
WO2022256422A1 (en) 2021-06-02 2022-12-08 10X Genomics, Inc. Sample analysis using asymmetric circularizable probes
EP4582555A3 (en) 2021-06-03 2025-10-22 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
EP4359555A1 (en) 2021-06-23 2024-05-01 Illumina, Inc. Compositions, methods, kits, cartridges, and systems for sequencing reagents
CA3224034A1 (en) 2021-06-25 2022-12-29 Helge Nareid Linear fourier fiducial
WO2022271689A1 (en) 2021-06-25 2022-12-29 Illumina, Inc. Fiducials for use in registration of a patterned surface
WO2023278966A1 (en) 2021-06-29 2023-01-05 Illumina, Inc. Machine-learning model for generating confidence classifications for genomic coordinates
WO2023278184A1 (en) * 2021-06-29 2023-01-05 Illumina, Inc. Methods and systems to correct crosstalk in illumination emitted from reaction sites
WO2023278609A1 (en) 2021-06-29 2023-01-05 Illumina, Inc. Self-learned base caller, trained using organism sequences
BR112023026615A2 (pt) 2021-06-29 2024-03-05 Illumina Inc Métrica de relação sinal-ruído para determinar chamadas de bases de nucleotídeos e qualidade de chamadas de bases
WO2023287617A1 (en) 2021-07-13 2023-01-19 Illumina, Inc. Methods and systems for real time extraction of crosstalk in illumination emitted from reaction sites
WO2023288225A1 (en) 2021-07-13 2023-01-19 10X Genomics, Inc. Methods for preparing polymerized matrix with controllable thickness
US11455487B1 (en) 2021-10-26 2022-09-27 Illumina Software, Inc. Intensity extraction and crosstalk attenuation using interpolation and adaptation for base calling
WO2023003757A1 (en) 2021-07-19 2023-01-26 Illumina Software, Inc. Intensity extraction with interpolation and adaptation for base calling
US20230021577A1 (en) 2021-07-23 2023-01-26 Illumina Software, Inc. Machine-learning model for recalibrating nucleotide-base calls
EP4377960A1 (en) 2021-07-28 2024-06-05 Illumina, Inc. Quality score calibration of basecalling systems
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
CA3224093A1 (en) 2021-07-30 2023-02-02 Jorge Ivan Hernandez Neuta Methods and compositions for synchronizing reactions in situ
US12460251B2 (en) 2021-08-03 2025-11-04 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid molecules
US12391984B2 (en) 2021-08-03 2025-08-19 10X Genomics, Inc. Compositions and methods for rolling circle amplification
KR20240035413A (ko) 2021-08-03 2024-03-15 일루미나, 인코포레이티드 다중 염기 호출자 모델을 사용한 염기 호출
CN118043476A (zh) 2021-08-03 2024-05-14 10X基因组学有限公司 核酸多联体及其稳定和/或压缩方法
EP4326898B1 (en) 2021-08-16 2024-07-31 10X Genomics, Inc. Probes comprising a split barcode region and methods of use
ES3011462T3 (en) 2021-09-01 2025-04-07 10X Genomics Inc Methods for blocking a capture probe on a spatial array
CN117580961A (zh) 2021-09-01 2024-02-20 Illumina公司 用于加速碱基判读的幅度调制
CN117561573A (zh) 2021-09-17 2024-02-13 因美纳有限公司 从碱基判读错误模式自动鉴定核苷酸测序中的故障来源
JP2024535664A (ja) 2021-09-21 2024-10-02 イルミナ インコーポレイテッド 帰属ハプロタイプを用いたグラフ参照ゲノム及び塩基コールアプローチ
US12480157B2 (en) 2021-09-30 2025-11-25 Illumina, Inc. Polynucleotide sequencing
EP4419707A1 (en) 2021-11-10 2024-08-28 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
EP4305195A2 (en) 2021-12-01 2024-01-17 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
KR20240116364A (ko) 2021-12-02 2024-07-29 일루미나, 인코포레이티드 뉴클레오티드 염기 호출을 결정하기 위한 클러스터별 신호 교정 생성
WO2023107622A1 (en) 2021-12-10 2023-06-15 Illumina, Inc. Parallel sample and index sequencing
WO2023122363A1 (en) 2021-12-23 2023-06-29 Illumina Software, Inc. Dynamic graphical status summaries for nucelotide sequencing
US20230215515A1 (en) 2021-12-23 2023-07-06 Illumina Software, Inc. Facilitating secure execution of external workflows for genomic sequencing diagnostics
WO2023129898A2 (en) 2021-12-27 2023-07-06 10X Genomics, Inc. Methods and compositions for rolling circle amplification
US20230207050A1 (en) 2021-12-28 2023-06-29 Illumina Software, Inc. Machine learning model for recalibrating nucleotide base calls corresponding to target variants
CA3242624A1 (en) 2021-12-29 2023-07-06 Shannon Whitmore Automatically switching variant analysis model versions for genomic analysis applications
EP4466376A1 (en) 2022-01-21 2024-11-27 10X Genomics, Inc. Multiple readout signals for analyzing a sample
US20240254537A1 (en) 2022-02-23 2024-08-01 Insitro, Inc. Pooled optical screening and transcriptional measurements of cells comprising barcoded genetic perturbations
AU2023225949A1 (en) 2022-02-25 2024-01-18 Illumina, Inc. Machine-learning models for detecting and adjusting values for nucleotide methylation levels
WO2023164660A1 (en) 2022-02-25 2023-08-31 Illumina, Inc. Calibration sequences for nucelotide sequencing
WO2023175021A1 (en) 2022-03-15 2023-09-21 Illumina, Inc. Methods of preparing loop fork libraries
AU2023234670A1 (en) 2022-03-15 2024-01-18 Illumina, Inc. Concurrent sequencing of forward and reverse complement strands on separate polynucleotides for methylation detection
US20230304086A1 (en) * 2022-03-28 2023-09-28 Illumina Cambridge Limited Labeled avidin and methods for sequencing
US20230313292A1 (en) 2022-03-29 2023-10-05 Illumina Cambridge Limited Chromenoquinoline dyes and uses in sequencing
AU2023243205A1 (en) 2022-04-01 2024-09-19 10X Genomics, Inc. Compositions and methods for targeted masking of autofluorescence
CN119234045A (zh) 2022-04-06 2024-12-31 10X基因组学有限公司 用于多重细胞分析的方法
WO2023196572A1 (en) 2022-04-07 2023-10-12 Illumina Singapore Pte. Ltd. Altered cytidine deaminases and methods of use
EP4515547A1 (en) 2022-04-26 2025-03-05 Illumina, Inc. Machine-learning models for selecting oligonucleotide probes for array technologies
WO2023209606A1 (en) 2022-04-29 2023-11-02 Illumina Cambridge Limited Methods and systems for encapsulating lyophilised microspheres
EP4519674A1 (en) 2022-05-06 2025-03-12 10X Genomics, Inc. Analysis of antigen and antigen receptor interactions
EP4523215A1 (en) 2022-05-10 2025-03-19 Illumina Inc. Adaptive neural network for nucelotide sequencing
JP2025516589A (ja) 2022-05-11 2025-05-30 10エックス ジェノミクス インコーポレイテッド インサイチュー配列決定のための組成物及び方法
WO2023232829A1 (en) 2022-05-31 2023-12-07 Illumina, Inc Compositions and methods for nucleic acid sequencing
US20240035071A1 (en) 2022-06-17 2024-02-01 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
US20230420080A1 (en) 2022-06-24 2023-12-28 Illumina Software, Inc. Split-read alignment by intelligently identifying and scoring candidate split groups
EP4544554A1 (en) 2022-06-27 2025-04-30 Illumina, Inc. Improved human leukocyte antigen (hla) genotyping
EP4544552A1 (en) 2022-06-27 2025-04-30 Illumina Inc. Accelerators for a genotype imputation model
US20230420082A1 (en) 2022-06-27 2023-12-28 Illumina Software, Inc. Generating and implementing a structural variation graph genome
EP4562638A1 (en) 2022-07-26 2025-06-04 Illumina, Inc. Rapid single-cell multiomics processing using an executable file
US20240033738A1 (en) 2022-07-27 2024-02-01 Illumina, Inc. Flow cell based motion system calibration and control methods
US20240101978A1 (en) 2022-08-12 2024-03-28 10X Genomics, Inc. Puma1 polymerases and uses thereof
WO2024040058A1 (en) 2022-08-15 2024-02-22 Element Biosciences, Inc. Methods for preparing nucleic acid nanostructures using compaction oligonucleotides
CN119677873A (zh) 2022-08-16 2025-03-21 10X基因组学有限公司 Ap50聚合酶及其用途
US20250361558A1 (en) 2022-08-19 2025-11-27 Illumina, Inc. Third dna base pair site-specific dna detection
EP4588051A1 (en) 2022-09-16 2025-07-23 Illumina, Inc. Cluster segmentation and conditional base calling
EP4590854A1 (en) 2022-09-19 2025-07-30 Illumina, Inc. Deformable polymers comprising immobilised primers
US20240100518A1 (en) 2022-09-26 2024-03-28 Illumina, Inc. Flow cell based motion system calibration and control methods
CN118974830A (zh) 2022-09-29 2024-11-15 因美纳有限公司 用于推算靶变体的靶变体参考组
CN119213083A (zh) 2022-09-30 2024-12-27 伊路米纳有限公司 用于减少测序期间的光损伤的组合物和方法
EP4595059A1 (en) 2022-09-30 2025-08-06 Illumina, Inc. Machine-learning model for refining structural variant calls
CN120153070A (zh) 2022-09-30 2025-06-13 因美纳有限公司 解旋酶-胞苷脱氨酶复合物及其使用方法
WO2024073043A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of using cpg binding proteins in mapping modified cytosine nucleotides
WO2024073047A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides
US20240127905A1 (en) 2022-10-05 2024-04-18 Illumina, Inc. Integrating variant calls from multiple sequencing pipelines utilizing a machine learning architecture
EP4602608A1 (en) 2022-10-11 2025-08-20 Illumina, Inc. Detecting and correcting methylation values from methylation sequencing assays
EP4602344A1 (en) 2022-10-14 2025-08-20 10X Genomics, Inc. Methods for analysis of biological samples
US20240167081A1 (en) 2022-11-08 2024-05-23 10X Genomics,Inc. Immobilization methods and compositions for in situ detection
WO2024107887A1 (en) 2022-11-16 2024-05-23 10X Genomics, Inc. Methods and compositions for assessing performance of in situ assays
US12372771B2 (en) 2022-11-18 2025-07-29 10X Genomics, Inc. Systems and methods for actively mitigating vibrations
EP4627583A1 (en) 2022-11-30 2025-10-08 Illumina, Inc. Accurately predicting variants from methylation sequencing data
US20240218437A1 (en) 2022-12-16 2024-07-04 10X Genomics, Inc. Methods and compositions for assessing performance
US20240218424A1 (en) 2022-12-21 2024-07-04 10X Genomics, Inc. Methods for tethering ribonucleic acids in biological samples
WO2024137886A1 (en) 2022-12-21 2024-06-27 Illumina, Inc. Context-dependent base calling
US20240229131A1 (en) 2022-12-22 2024-07-11 Illumina, Inc. Transition-metal catalyst compositions and methods for sequencing by synthesis
AU2023409219A1 (en) 2022-12-22 2024-10-03 Illumina, Inc. Palladium catalyst compositions and methods for sequencing by synthesis
US20240271206A1 (en) 2022-12-27 2024-08-15 Illumina, Inc. Methods of sequencing using 3' allyl blocked nucleotides
US20240263219A1 (en) 2023-01-06 2024-08-08 10X Genomics, Inc. Methods and compositions for in situ analysis of variant sequences
AU2024219208A1 (en) 2023-02-06 2025-01-16 Illumina, Inc. Determining and removing inter-cluster light interference
AU2024247902A1 (en) 2023-03-30 2024-12-12 Illumina, Inc. Compositions and methods for nucleic acid sequencing
WO2024206848A1 (en) 2023-03-30 2024-10-03 Illumina, Inc. Tandem repeat genotyping
WO2024216159A1 (en) * 2023-04-13 2024-10-17 Esbiolab Llc Methods and compositions for nucleic acid sequencing using labeled nucleotides
WO2024216163A1 (en) * 2023-04-13 2024-10-17 Esbiolab Llc Methods and compositions for nucleic acid sequencing using predominantly unlabeled nucleotides
CN119744419A (zh) 2023-05-03 2025-04-01 因美纳有限公司 用于重新校准来自现有测序数据文件的基因型检出的机器学习模型
US20240368678A1 (en) 2023-05-03 2024-11-07 10X Genomics, Inc. Methods and compositions for spatial assay
WO2024249200A1 (en) 2023-05-26 2024-12-05 Illumina, Inc. Methods for preserving methylation status during clustering
US20240404624A1 (en) 2023-05-31 2024-12-05 Illumina, Inc. Structural variant alignment and variant calling by utilizing a structural-variant reference genome
WO2024249961A1 (en) 2023-06-01 2024-12-05 Singular Genomics Systems, Inc. Methods and probes for detecting polynucleotide sequences in cells and tissues
WO2024249973A2 (en) 2023-06-02 2024-12-05 Illumina, Inc. Linking human genes to clinical phenotypes using graph neural networks
WO2024256581A1 (en) 2023-06-14 2024-12-19 Illumina, Inc. Determination of modified cytosines
WO2024256580A1 (en) 2023-06-14 2024-12-19 Illumina, Inc. Concurrent sequencing with spatially separated rings
WO2025006565A1 (en) 2023-06-27 2025-01-02 Illumina, Inc. Variant calling with methylation-level estimation
WO2025006460A1 (en) * 2023-06-30 2025-01-02 Illumina, Inc. Systems and methods of sequencing polynucleotides with modified bases
WO2025006874A1 (en) 2023-06-30 2025-01-02 Illumina, Inc. Machine-learning model for recalibrating genotype calls corresponding to germline variants and somatic mosaic variants
WO2025029831A1 (en) 2023-07-31 2025-02-06 10X Genomics, Inc. Methods and systems for targeted rna cleavage and target rna-primed rolling circle amplification
WO2025054389A1 (en) 2023-09-07 2025-03-13 Illumina, Inc. Identification of methylated cytosine using landmarks
WO2025050463A1 (en) 2023-09-08 2025-03-13 The Regents Of The University Of Michigan MICRORNA-DERIVED RNAs AND POLYPEPTIDES AND USES THEREOF
WO2025062002A1 (en) 2023-09-20 2025-03-27 Illumina, Inc. Concurrent sequencing using nick translation
WO2025061922A1 (en) 2023-09-20 2025-03-27 Illumina, Inc. Methods for sequencing
WO2025062001A1 (en) 2023-09-20 2025-03-27 Illumina, Inc. Optimised nucleic acid sequencing
WO2025061942A1 (en) 2023-09-20 2025-03-27 Illumina, Inc. Sequencing error identification and correction
WO2025072783A1 (en) 2023-09-28 2025-04-03 Illumina, Inc. Altered cytidine deaminases and methods of use
US20250111898A1 (en) 2023-09-29 2025-04-03 Illumina, Inc. Tracking and modifying cluster location on nucleotide-sample slides in real time
US20250111899A1 (en) 2023-09-29 2025-04-03 Illumina, Inc. Predicting insert lengths using primary analysis metrics
WO2025081064A2 (en) 2023-10-11 2025-04-17 Illumina, Inc. Thermophilic deaminase and methods for identifying modified cytosine
WO2025090883A1 (en) 2023-10-27 2025-05-01 Illumina, Inc. Detecting variants in nucleotide sequences based on haplotype diversity
US20250163502A1 (en) 2023-11-22 2025-05-22 10X Genomics, Inc. Methods for processing ribonucleic acids in biological samples
WO2025129133A1 (en) 2023-12-15 2025-06-19 Illumina, Inc. Minimal residual disease (mrd) models for determining likelihoods or probabilities of a subject comprising cancer
US20250201346A1 (en) 2023-12-18 2025-06-19 Illumina, Inc. Using machine learning models for detecting minimum residual disease (mrd) in a subject
WO2025137222A1 (en) 2023-12-19 2025-06-26 Illumina, Inc. Methylation detection assay
US20250207203A1 (en) 2023-12-20 2025-06-26 10X Genomics, Inc. In situ detection of copy number variations in biological samples
US20250210137A1 (en) 2023-12-20 2025-06-26 Illumina, Inc. Directly determining signal-to-noise-ratio metrics for accelerated convergence in determining nucleotide-base calls and base-call quality
WO2025137647A1 (en) 2023-12-21 2025-06-26 Illumina, Inc. Enhanced mapping and alignment of nucleotide reads utilizing an improved haplotype data structure with allele-variant differences
US20250215493A1 (en) 2023-12-28 2025-07-03 Illumina, Inc. Nucleotides with enzymatically cleavable 3'-o-glycoside blocking groups for sequencing
WO2025144626A1 (en) 2023-12-28 2025-07-03 Illumina, Inc. Sequencing with single-stranded binding proteins
WO2025160089A1 (en) 2024-01-26 2025-07-31 Illumina, Inc. Custom multigenome reference construction for improved sequencing analysis of genomic samples
WO2025174774A1 (en) 2024-02-12 2025-08-21 Illumina, Inc. Determining offline corrections for sequence specific errors caused by low complexity nucleotide sequences
WO2025174708A1 (en) 2024-02-13 2025-08-21 Illumina, Inc. Design and method for cross-sequencing platform compatibility of flow cells
WO2025184226A1 (en) 2024-02-28 2025-09-04 Illumina, Inc. Nucleotides with terminal phosphate capping
WO2025184234A1 (en) 2024-02-28 2025-09-04 Illumina, Inc. A personalized haplotype database for improved mapping and alignment of nucleotide reads and improved genotype calling
WO2025188906A1 (en) 2024-03-08 2025-09-12 Illumina, Inc. Modified adenosine nucleotides
WO2025193747A1 (en) 2024-03-12 2025-09-18 Illumina, Inc. Machine-learning models for ordering and expediting sequencing tasks or corresponding nucleotide-sample slides
WO2025190902A1 (en) 2024-03-13 2025-09-18 Illumina, Inc. Improving base calling quality scores
WO2025194033A1 (en) 2024-03-15 2025-09-18 10X Genomics, Inc. Systems and methods for covering and sealing an open well
WO2025230914A1 (en) 2024-04-29 2025-11-06 Illumina, Inc. Nucleotides with enzyme-triggered self-immolative linkers for sequencing by synthesis
WO2025240241A1 (en) 2024-05-13 2025-11-20 Illumina, Inc. Modifying sequencing cycles during a sequencing run to meet customized coverage estimations for a target genomic region
WO2025240924A1 (en) 2024-05-17 2025-11-20 Illumina, Inc. Blind equalization systems for base calling applications
WO2025240918A1 (en) 2024-05-17 2025-11-20 10X Genomics, Inc. Systems and methods for generating codebooks
WO2025250996A2 (en) 2024-05-31 2025-12-04 Illumina, Inc. Call generation and recalibration models for implementing personalized diploid reference haplotypes in genotype calling
WO2025255457A1 (en) 2024-06-07 2025-12-11 10X Genomics, Inc. Compositions and methods for improved on-target spatial profiling

Family Cites Families (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US781650A (en) 1904-08-15 1905-02-07 William Heap Commutator-brush holder.
US6051380A (en) 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5695934A (en) 1994-10-13 1997-12-09 Lynx Therapeutics, Inc. Massively parallel sequencing of sorted polynucleotides
EP0975802B1 (en) 1997-04-01 2004-06-23 Manteia S.A. Method of nucleic acid sequencing
DE69837913T2 (de) 1997-04-01 2008-02-07 Solexa Ltd., Saffron Walden Verfahren zur vervielfältigung von nukleinsäure
US5888737A (en) 1997-04-15 1999-03-30 Lynx Therapeutics, Inc. Adaptor-based sequence analysis
AU8162498A (en) 1997-06-25 1999-01-04 Orchid Biosciences, Inc. Methods for the detection of multiple single nucleotide polymorphisms in a single reaction
WO1999002671A1 (en) 1997-07-07 1999-01-21 Medical Research Council In vitro sorting method
DE69940430D1 (de) 1998-06-24 2009-04-02 Illumina Inc Dekodierung von matrixartig-angeordneten Sensoren durch Mikropartikel
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US7071324B2 (en) 1998-10-13 2006-07-04 Brown University Research Foundation Systems and methods for sequencing by hybridization
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
AU7537200A (en) * 1999-09-29 2001-04-30 Solexa Ltd. Polynucleotide sequencing
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
JP2004523243A (ja) * 2001-03-12 2004-08-05 カリフォルニア インスティチュート オブ テクノロジー 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US20040002090A1 (en) 2002-03-05 2004-01-01 Pascal Mayer Methods for detecting genome-wide sequence variations associated with a phenotype
EP1497304B1 (en) * 2002-04-12 2014-06-25 Catalyst Assets LLC Dual-labeled nucleotides
DE60327649D1 (de) 2002-08-23 2009-06-25 Illumina Cambridge Ltd Markierte nukleotide
US7414116B2 (en) * 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
DK3002289T3 (en) 2002-08-23 2018-04-23 Illumina Cambridge Ltd MODIFIED NUCLEOTIDES FOR POLYNUCLEOTIDE SEQUENCE
AU2004209426B2 (en) 2003-01-29 2008-04-17 454 Life Sciences Corporation Method for preparing single-stranded DNA libraries
US20050181394A1 (en) 2003-06-20 2005-08-18 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
EP1641809B2 (en) 2003-07-05 2018-10-03 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
GB0324456D0 (en) * 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
US7276338B2 (en) 2003-11-17 2007-10-02 Jacobson Joseph M Nucleotide sequencing via repetitive single molecule hybridization
DK1740100T3 (en) 2004-04-13 2016-11-28 Biosearch Tech Inc xanthene
EP1879906A4 (en) * 2005-01-05 2009-07-22 Advanced Genetic Analysis Corp REVERSIBLE NUCLEOTIDE TERMINATORS AND USES THEREOF
GB0507835D0 (en) 2005-04-18 2005-05-25 Solexa Ltd Method and device for nucleic acid sequencing using a planar wave guide
US20090264299A1 (en) 2006-02-24 2009-10-22 Complete Genomics, Inc. High throughput genome sequencing on DNA arrays
EP3257949A1 (en) 2005-06-15 2017-12-20 Complete Genomics Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
US20060287833A1 (en) 2005-06-17 2006-12-21 Zohar Yakhini Method and system for sequencing nucleic acid molecules using sequencing by hybridization and comparison with decoration patterns
US7514952B2 (en) 2005-06-29 2009-04-07 Altera Corporation I/O circuitry for reducing ground bounce and VCC sag in integrated circuit devices
GB0517097D0 (en) * 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
EP2495337A1 (en) 2006-02-24 2012-09-05 Callida Genomics, Inc. High throughput genome sequencing on DNA arrays
WO2007107710A1 (en) 2006-03-17 2007-09-27 Solexa Limited Isothermal methods for creating clonal single molecule arrays
EP3722409A1 (en) 2006-03-31 2020-10-14 Illumina, Inc. Systems and devices for sequence by synthesis analysis
WO2007135368A2 (en) * 2006-05-18 2007-11-29 Solexa Limited Dye compounds and the use of their labelled conjugates
JP2009542220A (ja) * 2006-06-30 2009-12-03 アプライド バイオシステムズ, エルエルシー 可逆性ターミネーターヌクレオチドおよび使用方法
WO2008043426A2 (de) * 2006-09-20 2008-04-17 Genovoxx Gmbh Komponenten und verfahren zur enzymatischen synthese von nukleinsäuren
WO2008042067A2 (en) * 2006-09-28 2008-04-10 Illumina, Inc. Compositions and methods for nucleotide sequencing
WO2008070352A2 (en) 2006-10-27 2008-06-12 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
EP2126766A2 (en) * 2007-01-26 2009-12-02 Illumina Inc. Image data efficient genetic sequencing method and system
EP2940029B1 (en) * 2007-10-19 2023-11-29 The Trustees of Columbia University in the City of New York Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis
EP2209911B1 (en) * 2007-10-19 2013-10-16 The Trustees of Columbia University in the City of New York Dna sequencing with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators and a deoxyinosine analogue with a reversible terminator group
WO2009097368A2 (en) * 2008-01-28 2009-08-06 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US7771903B2 (en) 2008-05-28 2010-08-10 Promos Technologies Pte. Ltd. Photolithography with optical masks having more transparent features surrounded by less transparent features
US20100092960A1 (en) 2008-07-25 2010-04-15 Pacific Biosciences Of California, Inc. Helicase-assisted sequencing with molecular beacons
US8772473B2 (en) * 2009-03-30 2014-07-08 The Regents Of The University Of California Mostly natural DNA sequencing by synthesis
CN101570784B (zh) * 2009-06-03 2011-11-23 东南大学 基于信号组合编码的dna连接测序方法
CN101565746B (zh) * 2009-06-03 2012-08-08 东南大学 带奇偶校验的信号组合编码dna连接测序方法
US9309569B2 (en) * 2010-08-26 2016-04-12 Intelligent Bio-Systems, Inc. Methods and compositions for sequencing nucleic acid using charge
PL3623481T3 (pl) * 2011-09-23 2022-01-17 Illumina, Inc. Kompozycje do sekwencjonowania kwasów nukleinowych
EP2844774B1 (en) 2012-05-02 2018-07-18 Ibis Biosciences, Inc. Dna sequencing

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