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EP3939702A1 - Pipette pour l' utilisation avec une pointe de pipette à plongeur intégré - Google Patents

Pipette pour l' utilisation avec une pointe de pipette à plongeur intégré Download PDF

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Publication number
EP3939702A1
EP3939702A1 EP21185307.2A EP21185307A EP3939702A1 EP 3939702 A1 EP3939702 A1 EP 3939702A1 EP 21185307 A EP21185307 A EP 21185307A EP 3939702 A1 EP3939702 A1 EP 3939702A1
Authority
EP
European Patent Office
Prior art keywords
tip
lifting rod
pipette
piston
locking sleeve
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP21185307.2A
Other languages
German (de)
English (en)
Inventor
Matthias KUNSCH
Burkhardt Reichmuth
Holger Link
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf SE filed Critical Eppendorf SE
Publication of EP3939702A1 publication Critical patent/EP3939702A1/fr
Pending legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0224—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type having mechanical means to set stroke length, e.g. movable stops
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/141—Preventing contamination, tampering
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/123—Flexible; Elastomeric

Definitions

  • the invention relates to a pipette for use with a pipette tip with an integrated piston.
  • the liquids can in particular be homogeneous (single-phase) liquids consisting of a single liquid component or of a homogeneous mixture of a plurality of liquid components (solutions). Furthermore, the liquids can be heterogeneous (multi-phase) mixtures of a liquid with another liquid (emulsions) or a solid (suspensions).
  • Pipettes have a stem-shaped pipette housing with a spigot (lug) at the lower end for clamping on a pipette tip.
  • the pin is often a conical, cylindrical or partially conical and cylindrical projection and is also referred to as a "working cone”.
  • a pipette tip is a hollow tube with a tip opening at the bottom and an attachment opening at the top, with which the pipette tip can be clamped onto the spigot.
  • the liquid is aspirated into and dispensed from the pipette tip.
  • the uptake and delivery of the liquid is controlled by the pipette.
  • Fixed-volume pipettes are used for pipetting constant volumes. With variable pipettes, the volume to be dosed can be adjusted.
  • a mechanical counter is used to display the set volume.
  • the stroke of a drive device can be adjusted by means of an adjustment device that is coupled to the counter.
  • the pipette tip is detached from the attachment after use and can be exchanged for a fresh pipette tip. In this way, cross-contamination can be avoided during subsequent pipetting.
  • Air-cushion pipettes have a piston-cylinder system in the pipette housing, which is connected via a channel to a through-hole in the spigot.
  • Pipette tips for air cushion pipettes do not have an integrated piston.
  • By displacing the piston by means of the drive device in the cylinder an air cushion is moved in order to suck liquid into and eject liquid from a pipette tip clamped onto the spigot.
  • a disadvantage of air cushion pipettes are dosing errors due to the change in the length of the air cushion due to the weight of the liquid sucked in and the influence of temperature, air pressure and humidity. Contamination of the pipette by aerosols can also be problematic.
  • Positive displacement pipettes are used with pipette tips with an integrated piston (positive displacement pipette tips).
  • Pipette tips with an integrated plunger have a tip plunger that is inserted into the pipette tip and can be pulled further out of the pipette tip to aspirate liquid and pushed deeper into the pipette tip to dispense liquid.
  • Positive displacement pipettes have a spigot for attaching the pipette tip and a drive device that can be coupled to the integrated piston (tip piston) for moving the piston.
  • the piston comes in direct contact with the liquid, eliminating the effects of an air pocket.
  • Positive displacement pipettes are particularly suitable for dispensing liquids with a high vapor pressure, high viscosity or high density and for applications in molecular biology where freedom from aerosols is important to avoid contamination.
  • Disposable or reusable air cushion or positive displacement pipette tips are made of plastic or glass.
  • said drive means has a lifting rod for moving a piston in a pipette tip which has a hollow lower lifting rod part and an upper lifting rod part inserted from above into the lower lifting rod part.
  • the upper lifting rod part is connected to a control element which protrudes from the upper end of the pipette housing.
  • a pipette tip Mastertip ® Eppendorf AG is clamped onto a pin of the pipette. By pressing the operating element, the lifting rod can be moved downwards, so that an upper end of the piston rod of a tip piston of the pipette tip is pressed into the lower lifting rod part.
  • a spring device is preloaded.
  • the spring device moves the lifting rod up to an upper stop, taking the tip piston with it and allowing liquid to be sucked into the pipette tip.
  • the liquid sucked in can be dispensed by pressing the operating element again down to the bottom stop.
  • the user has to press the operating element with increased force so that another spring device compresses, the upper part of the lifting rod is shifted down in the lower part of the lifting rod, the piston is pushed out of the lower part of the lifting rod and the pipette tip is pushed off the spigot.
  • the DE 27 11 124 C2 describes a pipette for use with a pipette tip with an integrated piston, which can be connected to a pipette tip via a bayonet lock.
  • the piston has a piston rod which is connected by a chuck of the pipette is held.
  • the collet In the closed position, the collet is pressed together by a tubular guide ring of the pipette so that it clamps around the piston rod.
  • the guide ring is moved upwards relative to the collet, the elasticity of the three-finger collet opens and releases the piston rod, allowing it to fall out loosely.
  • the frictional connection between the piston rod and the collet can become loose unintentionally.
  • the bayonet lock must be operated by hand and this can lead to contamination.
  • the U.S. 4,474,071 describes another pipette for use with a pipette tip with an integrated plunger, which has a spigot for attaching the pipette tip and a collet with elastically resilient fingers and a sleeve that can be slid onto the fingers for clamping the upper end of a plunger.
  • the frictionally fastened piston can come loose unintentionally.
  • the connection between the pipette and the plunger can require a great deal of force.
  • a helical spring is arranged between the fingers in order to elastically resiliently push them apart.
  • the object of the invention is to provide a pipette for use with a pipette tip with an integrated plunger, in which the forces for connecting the pipette to the tip plunger and for detaching the tip plunger from the pipette are reduced and the The tip piston is held sufficiently firmly on the pipette so that it does not detach from the pipette or move in its holder when pipetting highly viscous liquids or during rapid pipetting.
  • the first means for positively connecting the lifting rod and the second means for positively connecting the tip piston are designed to be complementary to one another, so that they form a positive connection with one another when the pipette tip is held on the spigot.
  • Connecting the tip piston to the lifting rod is facilitated by the fact that the lifting rod can be elastically expanded by the insertion of the upper end of the tip piston or the tip piston can be elastically expanded by the penetration of the lower end of the lifting rod.
  • the elastic expansion is caused by the forces acting between the first and second means for positive connection when telescoping the lower end of the lifting rod and the upper end of the tip piston.
  • the elastic widening is reversed in whole or in part when, when telescoping, the second means for positively connecting the tip piston form the positively connected connection with the first means for positively connecting the lifting rod. Thereafter, the at least one locking sleeve is shifted into the locking position. This secures the tip plunger to the lift rod since the locking sleeve in the locked position prevents the lower end of the lift rod or the upper end of the plunger rod from being elastically expanded, resulting in the disengagement of the positive connection between the lifting rod and the piston rod is required.
  • the locking sleeve is removed from the locked position so that it at least partially releases the lower end of the lifting rod or the upper end of the piston rod and the lower end of the lifting rod or the upper end of the piston rod can be elastically expanded.
  • the form-fitting connection can be released with little effort and the piston rod can be released from the lifting rod.
  • the lifting rod has at least one slot running in the longitudinal direction at the lower end and the locking sleeve delimits the lifting rod in the locking position, or the locking sleeve is designed to have at least one slot running in the longitudinal direction at the upper end and is positively connected to the lifting rod To limit the tip piston in the blocking position in the area of the slot on the outside.
  • the lifting rod has at least one slot running in the longitudinal direction for the elastic widening.
  • the lifting rod can consist of a hard-elastic or soft-elastic material, for example a metal or a plastic.
  • a lifting rod made of a flexible material for example silicone rubber, a thermoplastic elastomer or rubber
  • the tip piston has at least one slot running in the longitudinal direction at the upper end for the elastic widening.
  • the tip piston can consist of a hard-elastic plastic, for example polypropylene or polyethylene, or of a soft-elastic plastic. It is also possible to produce a tip piston, at least at the upper end, from a flexible material, for example silicone rubber, a thermoplastic elastomer or rubber, so that it has sufficient elasticity for elastic expansion even without a slit.
  • the tip piston can be produced from several materials by multi-component injection molding.
  • the locking sleeve is designed not to deform when loaded with a radial force sufficient to elastically expand the lift rod or the top of the tip piston.
  • the locking sleeve consists, for example, of metal or of a hard-elastic or rigid plastic.
  • the locking sleeve is connected to a first operating element which protrudes from the pipette housing and can be displaced relative to the pipette housing in order to move the locking sleeve downward into the locking position and upward out of the locking position by actuating the first operating element.
  • the shifting of the blocking element into the blocking position and out of the blocking position upwards is simplified for the user by the first operating element.
  • the locking sleeve is not connected to a first operating element, but can be moved by hand.
  • the pipette has an ejection device, which comprises an ejection rod, which is guided in a displaceable manner in the longitudinal direction of the spigot within an axial bore of the lifting rod and is arranged with its lower end above the tip piston when this is positively connected to the lifting rod, and which comprises a second operating element connected to the ejection rod in order to displace the ejection rod, the ejection device being designed to push the tip piston off the lifting rod by displacing the ejection rod downwards and to push off the pipette tip, which includes the tip piston, from the spigot when the locking sleeve reaches the lower end at least partially releases the lifting rod or the upper end of the tip piston.
  • an ejection device which comprises an ejection rod, which is guided in a displaceable manner in the longitudinal direction of the spigot within an axial bore of the lifting rod and is arranged with its lower end above the tip piston when this is positively connected to the lifting rod, and which comprises a second operating element connected to the ejection
  • the spigot has first means for form-fitting connection with a pipette tip, so that a pipette tip, which has second means for form-fitting connection of the pipette tip with the first means for form-fitting connection of the spigot, with elastic narrowing of the spigot and/or with elastic expansion of the pipette tip can be slid onto the spigot before it is positively connected to the spigot, at least one locking sleeve is arranged coaxially with the spigot and is guided in the pipette housing so that it can be displaced in the direction of the spigot, with the at least one locking sleeve being displaceable into a locking position in which it passes a Sliding a pipette tip on the inside and/or on the outside of a pipette tip that can be widened by pushing it onto the spigot, whereby the locking sleeve prevents a pipette tip that is positively connected to the spigot
  • the first means for positively connecting the spigot and the second means for positively connecting the pipette tip are designed to be complementary to one another, so that they form a positive connection with one another when the pipette tip assumes a specific position on the spigot.
  • the connection of the pipette tip to the spigot is made easier in that the spigot can be elastically narrowed by sliding the pipette tip on and/or the pipette tip can be elastically widened by the penetration of the spigot.
  • the elastic narrowing and/or widening is brought about by the forces acting between the first and second means for positive connection when the pipette tip is pushed onto the spigot.
  • the elastic narrowing and/or elastic widening is reversed in whole or in part if the second means for positively locking connection of the pipette tip enter into the positively locking connection with the first means for positively locking connection of the spigot when it is pushed on. After that, the at least one Locking sleeve shifted to the locked position. This secures the pipette tip on the spigot, since the locking sleeve in the locked position prevents the spigot from being elastically narrowed and/or the pipette tip from being elastically widened, which is necessary to release the positive connection between the spigot and the pipette tip.
  • the locking sleeve is removed from the locked position so that it at least partially releases the spigot and/or the pipette tip and the spigot can be narrowed elastically and/or the pipette tip can be widened elastically.
  • the form-fitting connection can be broken with little effort and the pipette tip can be detached from the spigot.
  • the peg has at least one slot running in the longitudinal direction and the locking sleeve limits the peg on the inside in the locking position and/or the locking sleeve is designed with a pipette tip that has at least one slit running in the longitudinal direction at the upper end and is positively connected to the peg to limit the locked position outside.
  • the pin for the elastic constriction has at least one longitudinal slot.
  • the pin can consist of a hard-elastic or soft-elastic material, for example a metal or a plastic.
  • a pin made of a flexible material, for example silicone rubber, a thermoplastic elastomer or rubber, can have sufficient elasticity for the elastic constriction even without a slit.
  • the pipette tip has at least one slot running in the longitudinal direction at the upper end for the elastic widening.
  • the pipette tip can consist of a hard-elastic plastic, for example polypropylene or polyethylene, or of a soft-elastic plastic. It is also possible to produce a pipette tip, at least at the upper end, from a flexible material, for example silicone rubber, a thermoplastic elastomer or rubber, so that it has sufficient elasticity even without a slit for the elastic expansion.
  • the pipette tip can be manufactured from several materials by multi-component injection molding. Here, the different materials can be positively and/or chemically connected to one another.
  • the locking sleeve is designed in such a way that it does not deform when subjected to a radial force that is suitable for elastically narrowing the spigot and/or elastically widening the pipette tip.
  • the locking sleeve consists, for example, of metal or of a hard-elastic or rigid plastic.
  • the locking sleeve is arranged between the lifting rod and the pin and, in the locked position, limits the lower end of the lifting rod or the upper end of the tip piston on the outside and at the same time the pin on the inside, as a result of which the locking sleeve prevents detachment of a tip piston connected to the lifting rod in a form-fitting manner of the lifting rod and at the same time prevents a pipette tip, which is positively connected to the spigot, from detaching from the spigot.
  • both the form-fitting connection of the tip piston with the lifting rod and the form-fitting connection of the pipette tip with the spigot are secured by the same locking sleeve.
  • the form-fitting connection of the tip piston with the lifting rod is secured by a first locking sleeve and the form-fitting connection of the pipette tip with the spigot is secured by a second locking sleeve.
  • the second locking sleeve can delimit the spigot on the inside or delimit a pipette tip placed on the spigot on the outside.
  • the locking sleeve and the ejector rod are connected to a gear mechanism, which includes the first operating element and the second operating element and is designed to control the displacement of the locking sleeve and the ejecting rod in opposite directions when at least one operating element is actuated, so that a form-fitting connection with the lifting rod connected tip piston by moving the ejector rod up and the locking sleeve downwards is prevented from detaching from the lifting rod and by moving the locking sleeve upwards and the ejector rod downwards the tip piston can be detached from the lifting rod and the pipette tip can be detached from the spigot.
  • a gear mechanism which includes the first operating element and the second operating element and is designed to control the displacement of the locking sleeve and the ejecting rod in opposite directions when at least one operating element is actuated, so that a form-fitting connection with the lifting rod connected tip piston by moving the ejector rod up and the locking sleeve downwards is prevented from det
  • the transmission device enables a synchronized displacement of the locking sleeve and the ejector rod in opposite directions in order to either hold the tip piston and, if necessary, the pipette tip securely on the pipette or to release the secure hold of the tip piston and, if necessary, the pipette tip and detach them from the pipette . This makes it easier for the user to use the pipette tip with integrated tip plunger.
  • the transmission device is designed to first move the locking sleeve upwards when pressing off a pipette tip and then to push off the tip piston from the lifting rod and the pipette tip from the attachment by moving the ejector rod downwards.
  • the tip piston is pressed off the lifting rod and the pipette tip off the spigot only after the release of the form-fitting hold of the tip piston on the lifting rod and possibly the pipette tip on the spigot.
  • the ejector rod is already pressing against the piston rod when the locking sleeve is still in the locking position.
  • the same control controls the displacement of the locking sleeve and the displacement of the ejector rod.
  • the first control element is also the second control element.
  • the same operating element controls the drive device.
  • the operating element drives the drive device.
  • the pipette has an operating element for actuating the locking sleeve and the ejection device and an additional operating element, different from this operating element, for controlling the drive device.
  • the transmission device comprises a cam carrier mounted rotatably in the pipette housing, a first scanning element guided on a first cam on the circumference of the cam carrier, which protrudes from an ejector rod, which is guided in the pipette housing so that it can be displaced in the longitudinal direction of the spigot, a first scanning element on a second curve on the circumference of the cam carrier, which protrudes from a control rod which protrudes upwards from the locking sleeve and together with the locking sleeve forms a locking device which can be displaced in the direction of the pin in the pipette housing, and an operating element which protrudes from the pipette housing and can be rotated relative to the pipette housing.
  • the cam carrier is designed in such a way that when the operating element is arranged in a starting position, the locking sleeve in a locking position delimits the lower end of the lifting rod or the upper end of the tip piston on the outside and, if necessary, delimits the pin on the inside and/or delimits the pipette tip on the outside, as a result of which the locking sleeve Detachment of a tip plunger connected in a form-fitting manner to the lifting rod and, if applicable, of a pipette tip connected in a form-fitting manner to the spigot, is prevented from the spigot, and the locking sleeve can be displaced upwards by turning the operating element, so that the lower end of the lifting rod or the upper end of the tip plunger is at least partially released and, if necessary, the spigot and/or the pipette tip is at least partially released and the ejection rod pushes the pipette tip off the spigot.
  • the gear device with a cam carrier can be designed as in the parallel European patent application of today's date of the same applicant entitled "Pipette for use with a pipette tip” with the application number EP 19 150 808.4 described. In this respect, reference is made to the above patent application, the content of which is hereby incorporated into the present patent application.
  • the drive device has a transmission mechanism which is designed to shift a drive element of the drive device alternately downwards and upwards during successive displacements of the operating element downwards, between which the operating element is displaced upwards.
  • This type of embodiment is advantageous in the case of an operating element which can be displaced in the longitudinal direction of the pin in order to drive the drive device. The first time the control element is moved down, the drive element is moved from an upper position down to a lower position, with the subsequent movement of the control element up, the drive element maintains its lower position, and with the subsequent movement of the control element down, the Drive element shifted back to the upper position. This sequence can be repeated any number of times.
  • the transmission mechanism is at least partially arranged within a curved carrier designed as a rotary sleeve. This enables space-saving accommodation.
  • the pipette housing and the transmission device have a magnet arrangement and/or a spring device which is designed to automatically move the operating element into the starting position.
  • the magnet arrangement includes, for example, two permanent magnets or one permanent magnet and a ferromagnetic component.
  • the permanent magnets or the ferromagnetic component are held on the pipette housing and on the gear mechanism in such a way that they automatically shift the operating element into a starting position when they come closer to one another.
  • This can also be achieved by a spring device which is preloaded when the gear device is displaced from the starting position and tends to shift the gear device back into the starting position.
  • a scanning element is present coaxially to the spigot, which is supported by a spring on the pipette housing and can be preloaded by pushing a pipette tip onto the spigot, so that when a pipette tip is detached from the spigot, the spring relaxes and the scanning element is able to push the pipette tip off the pipette cones supported.
  • the scanning element can in particular be a scanning element of a scanning device for scanning the collar of a pipette tip, as in FIG EP 18 168 763.3 described. In this respect, reference is made to the above patent application, the content of which is hereby incorporated into the present application.
  • the pipette is a single-channel pipette or a multi-channel pipette.
  • the cam carrier can control the ejector rod of a multi-channel ejector.
  • the first means for positively connecting the lifting rod to the tip piston are a circumferential annular groove or a ring-shaped circumferential bead at the lower end of the lifting rod.
  • the first means for the form-fitting connection are formed on the inner circumference of a receptacle at the lower end of the lifting rod or on the outer circumference of the lifting rod.
  • the second means for the positive connection of the tip piston are designed to be complementary to the first means for the positive connection with the tip piston.
  • the second means for positively connecting the tip piston is a circumferential annular groove on the upper end of the tip piston or a ring-shaped circumferential bead on the upper end of the tip piston.
  • the further means for the positive connection are formed on the outer circumference of the tip piston or on the inner circumference of a receptacle at the upper end of the tip piston.
  • the first means for the form-fitting connection of the spigot to a pipette tip are an annular groove running around the outside of the spigot or a bead running around the outside of the spigot in a ring-shaped manner.
  • the second means for positively connecting the pipette tip to the first means are designed to be complementary to the first means.
  • the second means for the positive connection of the pipette tip to the first means are a bead running around the inner circumference in a ring shape at the upper end of the pipette tip or an annular groove running around the inner circumference.
  • the lower end of the lifting rod denotes an area at the bottom of the lifting rod, which extends in the vertical direction over a more or less great length of the lifting rod.
  • the upper end of the tip piston denotes an area at the top of the tip piston, which extends in the vertical direction over a greater or lesser length of the tip piston.
  • the upper end of the pipette tip designates an area at the top of the pipette tip, which extends in the vertical direction over a more or less great length of the pipette tip.
  • the terms “above” and “below” as well as “vertical” and “horizontal” and terms derived therefrom such as “above” and “below”, “upright” and “upside down” and “ “Stacked” refers to an arrangement of the pipette in which the spigot is oriented vertically and is located at the downward-pointing end of the pipette body.
  • this information relates to a vertical orientation of the central axis of the pipette tip, with the tip opening being arranged at the bottom and the attachment opening at the top.
  • the pipette tip is additionally secured on the spigot by the same locking sleeve, in that it delimits the spigot on the inside.
  • the comments on the pipette from Figures 1 to 10 apply accordingly to the pipette according to Figures 12 to 14 .
  • a pipette 1 designed as a direct displacement pipette has a rod-shaped (eg cylindrical) pipette housing 2 .
  • a hollow-cylindrical shaft 3 projects downwards from the lower end of the pipette housing 2 .
  • From the lower end of the shaft 3 is a pin 4 down, according to 1 and 4 a through hole 5 with a through hole at the lower end.
  • the inner diameter of the through hole 5 is smaller than the inner diameter of the shank 3.
  • the spigot 4 has an upper spigot portion 6 in the shape of a hollow cylinder and below it a lower spigot portion 7 in the shape of a hollow cone.
  • An annular groove 8 runs around on the outer circumference of the pin 4 between the upper pin section 6 and the lower pin section 7 .
  • the upper spigot section 6, the annular groove 8 and the lower spigot section 7 form first means for positively locking connection 9 of the pipette to a pipette tip.
  • the pin 4 has slots 10 running in its longitudinal direction, which are evenly distributed over the circumference.
  • the slots 10 extend at the lower end 11 over the entire length of the pin 4.
  • a drive device 12 is present in the pipette housing 2 , which comprises a transmission element 13 in the form of a transmission rod 14 , a transmission mechanism 15 and a drive element 16 in the form of a lifting rod 17 . Furthermore, the drive device 12 comprises an operating element 18 in the form of an operating lever 19 which is firmly connected to a carrier plate 21 via a web 20 .
  • the support plate 21 has an oval shape with a wide rounded end and a narrow rounded end, with the operating lever 19 protruding from the edge of the narrow rounded end. Adjacent to this edge, the support plate 21 has a first curved slot 22 which runs approximately parallel to the contour of the narrow rounded end. Further, the support plate 21 has a rectangular first edge recess 23 at the center of the first curved slot 22 on the side of the narrow rounded end.
  • the lifting rod 17 is inserted into the shaft 3 and the pin 4 from above. According to 4 it is hollow and has an axial bore that extends to a hole in the lower end of the lifting rod. Furthermore, the Lifting rod 17 has a longitudinal slot 24 running from the lower end in the longitudinal direction. Due to the longitudinal slot 24, the lifting rod 17 has a C-shaped cross section. Its lower end forms a receptacle 25 for the upper end of a piston rod.
  • the transmission mechanism 15 is designed in such a way that with successive displacements of the operating lever 19 downwards, between which the operating lever 19 is displaced upwards, the lifting rod 17 is alternately displaced downwards and upwards.
  • the lifting rod 17 can be shifted from an upper position to a lower position, the lifting rod 17 maintains the lowered position with the subsequent upward displacement of the operating lever 19, and the lifting rod 17 is moved by a subsequent pushing of the Operating lever 19 shifted down again up. This can be repeated any number of times.
  • the pipette 1 is provided with a release device 26 .
  • This comprises a curved carrier 27 which is rotatably mounted in the pipette housing 2 and is designed as a hollow-cylindrical rotary sleeve 28 .
  • the rotary sleeve 28 is, for example, rotatably mounted with its outer circumference on the inner circumference of the pipette housing 2 and is supported at the upper and lower end between shoulders of steps on the inner circumference of the pipette housing 2, so that it cannot be displaced in the axial direction in the pipette housing 2.
  • the axis of rotation of the rotating sleeve 28 coincides with the longitudinal axis of the pipette housing 2 and the longitudinal axis of the pin 4 .
  • the rotary sleeve 28 has on two diametrically opposite sides parallel to its axis of rotation recesses 29, 30 which extend from the upper edge of the rotary sleeve 28 and end at a distance from its lower edge.
  • the rotary sleeve 28 consists of a circular base 31 and above it consists of two diametrically opposite sectors 32, 33 of a circular ring, which delimit the two recesses 29, 30 laterally.
  • the first cam 34 is formed as an inverted (upside down) Y-shaped first groove 36 .
  • the vertical part 37 of the Y extends well up on a sector 32 to just short of the top edge of the sector 32.
  • the second curve 35 is a second groove 38 on the outer periphery of the base 31 of the rotating sleeve 28 in the shape of an upright V.
  • the first curve 34 and the second curve 35 are offset from one another by 80°, for example, on the circumference of the rotary sleeve.
  • the first curve 34 and the second curve 35 each extend over an angular range of less than 90° over the circumference of the rotary sleeve 28.
  • the ejection device 26 includes an ejection rod 39 which comprises a strip-shaped upper ejection rod part 40 and a cylindrical lower ejection rod part 41 .
  • a correspondingly bent, continuous wire can also be used.
  • the upper and lower ejector rod parts 40, 41 are arranged parallel to one another and laterally offset from one another.
  • the lower end of the upper ejector rod part 40 is connected to the upper end of the lower ejector rod part 41 via a strip-shaped connecting rod part 42 inclined obliquely to the two ejector rod parts.
  • a first scanning element 43 in the form of a first guide pin 44 protrudes perpendicularly from the inside of the upper ejection rod part 40 .
  • the ejection rod 39 is preferably formed in one piece, for example from a rigid plastic.
  • the connecting rod part 42 extends through the longitudinal slot 24 of the lifting rod 17 and extends with the lower ejection rod part 41 inside the lifting rod 17 to just before the lower end thereof.
  • the pipette 1 includes a locking device 45 which includes a locking sleeve 46 and a strip-shaped control rod 47 parallel thereto.
  • the upper end of the locking sleeve 46 and the lower end of the control rod 47 are connected to each other via a second connecting rod part 48 which is inclined obliquely to the locking sleeve 46 and the control rod 47.
  • a second sensing element 49 in the form of a second guide pin 49.1 protrudes from the inside of the control rod 47.
  • the second guide pin 49.1 is guided in the second groove 38.
  • the locking sleeve 46 is inserted into the shank 3 from above and lies against the inside of the pin 4 .
  • the lifting rod 17 and the ejection rod 39 are inserted into the locking sleeve 46 from above.
  • the operating element 18 is pushed with the first curved slot 22 onto the sector 32 of the rotary sleeve 28 on which the first groove 36 extends.
  • the rotary sleeve 28 is connected at the top to a support ring 50 which bridges the two sectors 32, 33 and stabilizes the rotary sleeve 28.
  • the support ring 50 has a jacket 51 projecting downwards at the outer edge, which surrounds the two sectors 32, 33 laterally at the outer edges. It also has a second curved slot 52 which receives the upper edge of the sector 33 which is not provided with a groove 36,38. On the diametrically opposite side, there is a downwardly opening, rectangular second edge recess 52 .
  • the support ring 50 is connected to the rotary sleeve 28 by gluing, for example.
  • the rotating sleeve 28 and the locking sleeve 46 and the operating element 18 are made, for example, from one or more rigid plastics and/or from metal.
  • the rotary sleeve 28, the support ring 50, the operating element 18 and/or the locking sleeve 46 are preferably each formed in one piece.
  • a control button of Operating element 18 can also be made of an elastic or flexible plastic or rubber.
  • the operating lever 19 protrudes from the pipette housing 2 through a first housing slot which runs transversely to the longitudinal axis of the pipette housing 2 and extends over part of the circumference of the pipette housing 2, so that it can be operated from the outside.
  • the first housing slot is connected in the middle to a second housing slot running in the longitudinal direction of the pipette housing 2 .
  • the operating lever 19 can be displaced downwards from the support ring 50 along the second housing slot against the action of a spring device, sliding with the first curved slot 22 on the sector 32 of the rotary sleeve 28 . After the load has been released, the spring device automatically moves the operating lever 19 back up.
  • a sleeve-shaped third scanning element 53 is guided on the outside of the shaft 3 .
  • a spring device in the form of a helical spring 54 guided on the shaft is supported on the underside of the pipette housing 2 and on the upper side of the third scanning element 53 .
  • the third scanning element 53 is pressed from above against a stop element on the shaft 3 or pin 4 by the coil spring 54 .
  • a setting button 55 for setting a dosing volume.
  • the dosing volume can be adjusted by turning the adjusting knob 55 .
  • a counter 56 arranged underneath in the pipette housing 2 indicates the dosing volume set in each case.
  • the setting button 55 and/or the counter 56 is coupled to the transmission mechanism 15 .
  • the transmission mechanism 15 is designed to change the stroke of the lifting rod 17, which is carried out by moving the operating element 18 downwards, in accordance with the dosing volume set in each case.
  • a pipette tip 57 is attached to the pin 4 .
  • the pipette tip 57 comprises a tubular body 58 having a tip opening 59 at the lower end, a collar 61 having a fitting opening 60 at the upper end, and a seating area 62 on the inner periphery of the collar 61 for clamping onto the spigot 4 .
  • the seat area 62 has a contour complementary to the pin 4, which has a conical lower seat section 63 for receiving the conical lower pin section 7, a circumferential bead 64 above it for engaging in the annular groove 8 of the pin 4 and above this a cylindrical upper seat section 65 for receiving the cylindrical upper pin portion 6 has.
  • the lower seat section 63, the bead 64 and the upper seat section 65 form second means for positively locking connection 66 of the pipette tip 57 to the pipette 1.
  • the tubular body 58 has a cylindrical piston running area 67 .
  • the tubular body 58 has a downwardly tapering tip portion 68 in the shape of a hollow truncated cone.
  • the tip section 68 is in 4 shown and omitted in the remaining drawings for the sake of simplicity.
  • a tip piston 69 is inserted in the tubular body 58 .
  • This includes a piston 70 which is guided in the piston running area 67 .
  • a piston rod 71 having a smaller diameter than the piston 70 projects upward.
  • the piston rod 71 has a piston head 72 at the upper end.
  • the piston head 72 is according to 4 pressed down into the receptacle 25 of the lifting rod 17 .
  • the pipette 1 can be used as follows: According to 1 and 8th a pipette tip 57 is held on the pipette 1 in a pipetting position.
  • the seating area 62 is positively connected to the pin 4 in particular by the engagement of the bead 64 in the annular groove 8 .
  • the operating element 18 is in the pipetting position at the upper end of the second housing slot, in which it can be rotated in both directions into the first housing slot.
  • the maximum angle of rotation is limited by the circumferential extent of the first and second grooves 36, 38 or the first housing slot, whichever is less.
  • the locking sleeve 46 is in accordance with 4 arranged in the lowest position so that it prevents accidental detachment of the pipette tip 57 from the spigot 4. A radial constriction of the pin 4 would be required for the dissolution of the form-fitting connection, which the locking sleeve 46 does not permit in this position.
  • the tip piston 69 is clamped with the piston head 72 in the receptacle 25 of the lifting rod 17.
  • the pipette 1 is immersed in a liquid with the lower end of the pipette tip 57 held thereon. Thereafter, the operating element 18 is pressed down. This movement is converted into a lifting movement of the lifting rod 17 by the transmission device 15 . As a result, the tip piston 69 is displaced upward.
  • the piston head 72 takes the ejector rod 39 with it, so that the first guide pin 44 slides upwards in the vertical part 37 of the Y-shaped first groove 36 . Meanwhile, the locking sleeve 46 maintains its position. this is in 9 shown.
  • the pipette tip 57 is filled with a certain amount of liquid. Thereafter, the operating element 18 is relieved and shifted back up by the spring device until it rests on the support ring 50 . To dispense this amount of liquid, the pipette 1 can be aligned with the pipette tip 57 towards another vessel. By pressing the operating element 18 down again, the lifting rod 17 is shifted down and the amount of liquid is dispensed. Here, the first guide pin 44 slides down to the node of the first groove 36.
  • the operating element 18 executes an operating stroke that depends on the set amount of liquid.
  • the operating stroke for dispensing the liquid is always the full operating path, regardless of the set amount of liquid.
  • the uptake and delivery of liquid can be carried out several times.
  • the operating lever 18 is pivoted to the right or to the left from the pipetting position. This rotates the rotary sleeve 28 so that the second groove 38 moves the second guide pin 49.1 and thus the locking sleeve 46 upwards until the locking sleeve 46 has released the pin 4 to such an extent that it can be deformed radially inwards.
  • the lower end of the lifting rod 17 is additionally released from the locking sleeve 46 to such an extent that it can be deformed radially outwards.
  • the locking sleeve 46 is preferably pulled out of the through hole 5 .
  • the first guide pin 44 is displaced downwards in one of the two lateral sections of the lower part of the first groove 36, so that the ejector rod 39 presses against the tip piston 69, which is supported on the tip section 68 at the bottom.
  • the bead 64 exerts a radial force on the pin, so that it narrows and the positive connection between the pipette tip 57 and the pin 4 is broken. As a result, the pipette tip 57 is released from the peg 4 . this is in 10 shown.
  • the annular groove 77 or the thickening 75 exerts a radial force on the lifting rod 17 so that it expands and the form-fitting connection between the tip piston 69 and the lifting rod 17 is broken.
  • the stripping of the pipette tip 57 from the peg 4 can also be assisted by the scanning element 53 which is pressed against the upper edge of the pipette tip 57 by the prestressed helical spring 54 .
  • a new pipette tip 57 can be connected to the spigot 4.
  • the pipette 1 with the spigot 4 can be inserted into the receiving opening 68 of a pipette tip 57 held ready in a carrier.
  • the scanning element 53 is shifted upwards and the helical spring 54 is prestressed.
  • the piston head 72 presses against the underside of the ejection rod 39 so that the first guide pin 44 slides up to the first branching point of the first groove 36 .
  • the rotary sleeve 28 is rotated in the pipette housing 2 until the operating element 18 is in the pipetting position.
  • the locking sleeve 46 is in the locking position of 4 shifted, in which it prevents detachment of the pipette tip 57 from the pin 4. In the embodiment of Figures 12 to 14 In the locked position, the locking sleeve 46 also prevents the tip piston 69 from detaching from the lifting rod 17.
  • connection of the tip plunger 69 to the lifting rod 17 and the pipetting can take place in the manner previously described.
  • the embodiment of 11 differs from that previously described in that the locking sleeve 46 is pushed over the outer circumference of the collar 61 of the pipette tip 57 in the locking position.
  • pipette tips 57 are used which have at least one longitudinal slit starting from the upper end. The longitudinal slit allows the pipette tip 57 to expand radially in order to establish the form-fit connection of the pipette tip 57 with the spigot 4 . The dissolution of the positive connection is prevented when the locking sleeve 46 is in the locking position, as in FIG 11 shown.
  • the locking sleeve 46 is displaced upwards by means of the rotating sleeve 28, as in the previously described embodiment, and then the pipette tip 57 is detached from the spigot 4 by pressing against the upper side of the tip piston 69.
  • a pipette 1 comprises a pin 4 with slots 10 extending from the lower end, a lifting rod 17 with further slots 73 extending from the lower end and a locking sleeve 46 with a control rod 47 at the upper end.
  • the pin 4 has a circumferential annular groove 8 in the area of the slots 10 .
  • a matching pipette tip 57 and a matching tip plunger 69 are also shown.
  • the pipette tip 57 has a tubular body 58 with a tip opening 59 at the lower end and an attachment opening 60 at the upper end.
  • the pipette tip 57 has a collar 61 with a seating area 62 on the inner circumference in which an inwardly projecting bead 64 runs.
  • the pipette tip 57 has a plunger running area 67 on the inside. This includes a piston 70 which is guided in the piston running area 67 .
  • a piston rod 71 which has a smaller diameter than the piston 70 projects upwards from the piston 70 .
  • the piston rod 71 has a piston head 72 at the upper end.
  • the piston 70 has a downwardly tapering cone 74 which is used to center the tip piston 69 at the top of the piston barrel portion 67 when it is inserted into the body 58 as far as it will go.
  • the piston head 72 has a cylindrical thickening 75 with a ramp 76 at the upper end. Under the thickening 75 another annular groove 77 runs around.
  • the segments of the lift rod 17 remaining between the slots 73 have bottom inwardly projecting hooks 78 shaped and dimensioned to snap into the annular groove 77 on the piston head 72 .
  • this arrangement is inserted into the pin 4.
  • the lifting rod 17, the locking sleeve 46 and the pin 4 are shaped and dimensioned in such a way that in 13c shown locking position of the locking sleeve 46, the lifting rod 17 in the area of the slots 73 can not be further elastically expanded and the pin 4 in the area of the slots 10 can not be further elastically compressed.
  • the hooks 78 engage in the annular groove 77 of the tip piston 69
  • the locking sleeve 46 thus prevents a tip piston 69 from becoming detached from the lifting rod 17.
  • a pipette tip held in the annular groove 8 of the spigot 4 on a bead 64 on the inner circumference of the body 58 is prevented from becoming detached by the locking sleeve 46 .
  • Figure 1 shows the upper end of tip piston 69 and the lower end of lift rod 17 prior to assembly.
  • the piston head 72 is inserted into the opening at the lower end of the lifting rod 17, the lower end of the lifting rod 17 being gradually expanded as the hooks 78 slide over the ramp 76.
  • the elastic expansion of the locking sleeve 46 is facilitated by the slits 73 .
  • the hooks 78 slide over the thickening 75 and snap into the further annular groove 77 . This is in the Figure 14b shown.
  • the tip piston 69 is secured to the lift rod 17 by sliding the locking sleeve 46 over the lower end with the slots 73, since the locking sleeve 46 prevents elastic expansion of the lower end.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Sampling And Sample Adjustment (AREA)
EP21185307.2A 2020-07-14 2021-07-13 Pipette pour l' utilisation avec une pointe de pipette à plongeur intégré Pending EP3939702A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102020118587 2020-07-14

Publications (1)

Publication Number Publication Date
EP3939702A1 true EP3939702A1 (fr) 2022-01-19

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ID=76920568

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Application Number Title Priority Date Filing Date
EP21185307.2A Pending EP3939702A1 (fr) 2020-07-14 2021-07-13 Pipette pour l' utilisation avec une pointe de pipette à plongeur intégré

Country Status (4)

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US (1) US12303883B2 (fr)
EP (1) EP3939702A1 (fr)
JP (1) JP7315625B2 (fr)
CN (1) CN113926500B (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2607893A (en) * 2021-06-11 2022-12-21 Spt Labtech Ltd Pipette tip for a liquid dispensing apparatus

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3680017B1 (fr) * 2019-01-08 2021-07-21 Eppendorf AG Pipette destinée à une utilisation avec une pointe de pipette
DE102022104546A1 (de) * 2022-02-25 2023-08-31 Eppendorf Se Pipette mit verstellbarem Pipettiervolumen und Verriegelungssystem
CN114955953B (zh) * 2022-06-24 2024-06-11 立臻精密智造(昆山)有限公司 一种维修工具
EP4331725A1 (fr) * 2022-08-30 2024-03-06 Eppendorf SE Tige de piston et procédé de fabrication d'une telle tige de piston

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2383704A1 (fr) * 1977-03-15 1978-10-13 Labora Mannheim Gmbh Pipette a main
US4474071A (en) 1981-10-28 1984-10-02 Marteau D Autry Eric Pipette
US5192511A (en) * 1991-05-31 1993-03-09 Tri-Continent Scientific, Inc. Pipette tip and piston
US5770160A (en) * 1995-09-15 1998-06-23 Bio-Plas, Inc. Positive displacement liquid drawing and dispensing apparatus
US20160271602A1 (en) 2013-11-07 2016-09-22 Gilson Sas Positive displacement pipetting system, having a design facilitating the gripping of the piston of the capillary-piston assembly
US20200215529A1 (en) * 2019-01-08 2020-07-09 Eppendorf Ag Pipette for use with a pipette tip

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI46688C (fi) * 1971-06-23 1973-06-11 Suovaniemi Pipetti
FR2295405A1 (fr) * 1974-10-29 1976-07-16 Marteau D Autry Eric Pipette etalonnable
FR2489507B1 (fr) * 1980-08-27 1986-05-02 Hettich Andreas Pipette doseuse pour le debit de quantites predeterminees de liquide, procede d'etablissement de son organe d'entrainement et dispositif comprenant de telles pipettes
US4418580A (en) * 1981-10-13 1983-12-06 Sherwood Medical Company Pipettor mechanism and disposable tip and piston assembly
CH671526A5 (fr) 1985-12-17 1989-09-15 Hamilton Bonaduz Ag
WO1991005609A1 (fr) * 1989-10-20 1991-05-02 Costar Corporation Pipette
FI116612B (fi) * 2004-07-05 2006-01-13 Biohit Oyj Imulaite
WO2007097229A1 (fr) * 2006-02-20 2007-08-30 Shimadzu Corporation Dispositif de réaction
DE102007010299B4 (de) * 2007-03-02 2009-01-29 Eppendorf Ag Handpipettiervorrichtung
DE102008048252A1 (de) * 2008-09-12 2010-04-15 Eppendorf Ag Pipettiervorrichtung
EP2458226A1 (fr) * 2010-11-29 2012-05-30 Magnetbau Schramme GmbH & Co. KG Dispositif de verrouillage
FI123652B (fi) * 2011-10-20 2013-08-30 Thermo Fisher Scientific Oy Lukittava pipetti
US9027419B2 (en) * 2012-02-07 2015-05-12 Eppendorf Ag Pipette
DE102012002169B4 (de) * 2012-02-07 2017-02-09 Eppendorf Ag Pipette
DE102012003846B4 (de) * 2012-02-29 2014-12-11 Eppendorf Ag Pipette
PL2659978T3 (pl) * 2012-05-02 2017-11-30 Eppendorf Ag Pipeta z systemem blokowania
EP2789388A1 (fr) * 2013-04-12 2014-10-15 Biocartis SA Pilote de collecteur
KR20150115391A (ko) * 2014-04-04 2015-10-14 쓰리엠 이노베이티브 프로퍼티즈 캄파니 피펫 장치
FR3040896B1 (fr) * 2015-09-15 2017-10-13 Gilson Sas Pipette de prelevement comprenant un organe de commande a double fonction d'ejection de cone et de deverrouillage du systeme de reglage de volume
CN205672929U (zh) * 2016-06-13 2016-11-09 山东大学 一种新型移液器
PL3399215T3 (pl) * 2017-05-05 2021-01-25 Eppendorf Ag Napęd wrzeciona
CN209302791U (zh) * 2018-10-17 2019-08-27 广州贝特生物科技有限公司 一种可调数字的多道移液器
EP3680017B1 (fr) * 2019-01-08 2021-07-21 Eppendorf AG Pipette destinée à une utilisation avec une pointe de pipette

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2383704A1 (fr) * 1977-03-15 1978-10-13 Labora Mannheim Gmbh Pipette a main
DE2711124C2 (de) 1977-03-15 1979-05-10 Labora Mannheim Gmbh Fuer Labortechnik, 6800 Mannheim Handpipette
US4474071A (en) 1981-10-28 1984-10-02 Marteau D Autry Eric Pipette
US5192511A (en) * 1991-05-31 1993-03-09 Tri-Continent Scientific, Inc. Pipette tip and piston
US5770160A (en) * 1995-09-15 1998-06-23 Bio-Plas, Inc. Positive displacement liquid drawing and dispensing apparatus
US20160271602A1 (en) 2013-11-07 2016-09-22 Gilson Sas Positive displacement pipetting system, having a design facilitating the gripping of the piston of the capillary-piston assembly
US20200215529A1 (en) * 2019-01-08 2020-07-09 Eppendorf Ag Pipette for use with a pipette tip

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2607893A (en) * 2021-06-11 2022-12-21 Spt Labtech Ltd Pipette tip for a liquid dispensing apparatus

Also Published As

Publication number Publication date
JP2022018092A (ja) 2022-01-26
JP7315625B2 (ja) 2023-07-26
CN113926500B (zh) 2023-05-09
US20220016618A1 (en) 2022-01-20
US12303883B2 (en) 2025-05-20
CN113926500A (zh) 2022-01-14

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