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WO1991005609A1 - Pipette - Google Patents

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Publication number
WO1991005609A1
WO1991005609A1 PCT/US1990/005800 US9005800W WO9105609A1 WO 1991005609 A1 WO1991005609 A1 WO 1991005609A1 US 9005800 W US9005800 W US 9005800W WO 9105609 A1 WO9105609 A1 WO 9105609A1
Authority
WO
WIPO (PCT)
Prior art keywords
pipetter
adjusting screw
piston
spindle
stripper
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1990/005800
Other languages
English (en)
Inventor
Anthony M. Labriola
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Costar Corp
Original Assignee
Costar Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Costar Corp filed Critical Costar Corp
Priority to DE69008540T priority Critical patent/DE69008540T2/de
Priority to EP90915595A priority patent/EP0496784B1/fr
Publication of WO1991005609A1 publication Critical patent/WO1991005609A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0224—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type having mechanical means to set stroke length, e.g. movable stops

Definitions

  • This invention relates to pipetters used to transfer fluid from one location to another and more particularly comprises a new and improved manually operated, hand-held pipetter.
  • the principle object of the present invention is to devise such a device which is free of expensive specially machined metal parts and which may be easily assembled and disassembled so as to reduce the manufacturing costs of the device and enable the user to assemble and disassemble it conveniently for cleaning and autoclaving.
  • Another important object of the present invention is to provide a pipetter which may easily and conveniently be held in one hand and which may be adjusted, locked, and actuated by the hand which holds it.
  • Another important object of the present invention is to provide a variable volume pipetter which may be easily and conveniently locked to repeatedly transfer a selected volume and which will not thereafter be accidentally unlocked so as to
  • the device provides a pipetter with reliable repeatability.
  • Another important object of the present invention is to provide a vernier scale in the volume adjustment assembly, which is convenient to use and easy to read and which does not interfere with the use of the device.
  • the pipetter of the present invention comprises an elongated body adapted to be held in one hand with the palm and fingers encircling it.
  • An adjusting screw is mounted in the body and extends through a major portion of its length, and the adjusting screw is externally threaded.
  • a tip holder is mounted on the body in alignment with the adjusting screw and both have internal passages which extend axially in the body and together form a continuous passage.
  • a pipetter piston is mounted in the adjusting screw.
  • a thumb actuator connected to the pipetter piston extends above the body and is positioned to be actuated by the thumb as the body is held in the palm and four fingers.
  • a spindle rotatable on the adjusting screw carries a stop which controls the metering of the pipetter. By rotating the spindle, the stop is moved so as to vary the length of the stroke of the piston and thus adjust the quantity of liquid drawn into the device.
  • a vernier is provided on the spindle which may be read through a window in the body. The window also affords access to the spindle so that it may be turned.
  • a slide lock is mounted on the body controlled by a finger grip exposed above the lower end of the body.
  • the lock is positioned to engage the spindle and bind against it so as to prevent spindle rotation when the lock slide is in the operative position.
  • a stripper surrounds the tip holder and extends substantially to the lower end thereof.
  • An actuator for the stripper is mounted in the body, and the stripper and actuator are
  • a thumb actuated button is carried on the top end of the actuator so as to be selectively operated by the user when a removable tip is to be stripped from the holder.
  • FIG. 1 is a perspective view of a pipetter constructed in accordance with this invention and shown as it may be held in the hand of a user;
  • FIG. 2 is an enlarged front elevation view of the pipetter shown in FIG. 1;
  • FIG. 3 is a top view of the pipetter
  • FIG. 4 is a longitudinal cross-sectional view of the pipetter taken along section line 4-4 in FIG. 3;
  • FIG. 5 is an enlarged fragmentary cross sectional view of the pipetter taken along section line 5-5 in FIG. 4;
  • FIG. 6 is a cross-sectional view of the pipetter taken along section line 6-6 in FIG. 4 and showing the spindle of the device in the locked position;
  • FIG. 7 is a cross-sectional view taken along section line 7-7 in FIG. 6 but showing the spindle of the pipetter in the unlocked position;
  • FIG. 8 is a cross-sectional view of the assembled pipetter taken along the section line 8-8 in FIG. 4;
  • FIG. 9 is a cross-sectional view similar to FIG. 8 but showing the pipetter in the partially assembled form
  • FIGS. 10 and 11 are fragmentary cross-sectional views similar to FIG. 5 but showing the pipetter piston in the partially and fully depressed positions, respectively;
  • FIG. 12 is an exploded view of the pipetter showing the major components of the pipetter and suggesting how they are assembled together;
  • FIGS. 13-17 are diagrammatic views showing the sequence of steps through which the pipetter is operated to dispenser metered quantities of liquid and to strip a tip from the tip holder.
  • the pipetter of the present invention has a generally tubular body 20 carrying a cap 22 at its upper end 24 and a tip holder 26 at its bottom end (see FIGS. 1 and 2).
  • the body 20 houses both the mechanism for performing the pipetting action and the stripping mechanism for removing a disposable tip T from the holder.
  • the orientation of the parts of the pipetter are described with respect to the normal, generally vertical position of the instrument in use.
  • the cap 22 is ultrasonically welded or otherwise permanently secured to the body 20 and has a curved flange 28 which normally engages the index finger of the user's hand when the instrument is held in the operative position with the operator's thumb positioned to actuate either of the two pistons in the device, which are described more fully below.
  • the normal gripping position of the cap 22 is ultrasonically welded or otherwise permanently secured to the body 20 and has a curved flange 28 which normally engages the index finger of the user's hand when the instrument is held in the operative position with the operator's thumb positioned to actuate either of the two pistons in the device, which are described more fully below.
  • the tip holder 26 at its upper end 34 has an enlarged diameter, and at its extreme upper end carries an outwardly extending flange 36 which is engaged by the lip 38 on the lower end of locknut 32.
  • the cylindrical chamber 40 within the top end 34 of holder 26 carries an O-ring 42 held in place by an O-ring retainer 44 also disposed in the enlarged top end 34.
  • the O-ring retainer 44 has an outwardly extending flange 46 at its top, which abuts against the flange 36 of the tip holder.
  • the locknut 32 retains the subassembly comprising the O-ring retainer 44 and tip holder 26 on adjusting screw 48 that forms part of the metering assembly that is described in detail below.
  • a piston 50 and guide rod 52 (hereinafter sometimes collectively called the pipetter piston 53) are coupled together at 54 and move reciprocally together within the body 20 and tip holder 26.
  • the guide rod 52 at its upper end carries a stem 56 which, in turn, carries a plunger button 58 (see FIGS. 4 and 12).
  • the plunger button 58 is positioned to be engaged by the thumb of the hand as suggested in FIG. 1 so as to move the pipetter piston 53 axially and downwardly in the body 20 and tip holder 26 when fluid is to be expelled from the cylindrical passage 60 of the tip holder 26 and the axial passage 61 in the disposable tip T.
  • the length of the vertical stroke of the pipetter piston 53 is controlled by two separate stops mounted in the body 20.
  • the upper limit of the stroke of the pipetter piston is established by the ' adjustable stop 70 defined by the flange 72 of a spindle cap 74 which in turn is carried by a spindle 76.
  • the spindle 76 As is evident in FIGS. 4 and 5, the spindle 76
  • the second stop 80 is established by a washer 82 which engages the shoulder 112 of the piston assembly 53.
  • the spindle cap 74 and spindle 76 are separately fabricated.
  • the spindle 76 at its upper end 84 has inner and outer collars 86 and 88 which define an annular space 90 between them into which the collar 92 on the lower end of the spindle cap 74 extends (see FIG. 5).
  • the two parts may be ultrasonically welded together or otherwise secured together so that they are permanently fixed to one another and move as a unit on the adjusting screw 48.
  • the flange 72 of the spindle cap 74 lies above the shoulder 94 of the guide rod 52 and is provided with a small opening 96 at its center through which the stem 56 extends (see FIG. 4).
  • the position of the flange may be varied by rotating spindle 76 on the adjusting screw 48.
  • the spindle 76 may be viewed within the body 20 of the pipetter through a window 100 (see FIG. 2), and the spindle carries a ring 102 with a vernier scale which cooperates with a scale 104 in the window 100.
  • These calibrations are a measure of the volume of fluid to be drawn into the passage 61 in the tip T when the pipetter piston 53 is retracted, as is made clear below in connection with the description of the operation of the instrument.
  • the lower stop 80 disposed within the axial chamber 110 in adjusting screw 48 cooperates with the shoulder 112 formed in the guide rod 52 and which faces downwardly in the pipetter body 20.
  • the washer 82 that comprises the stop is yieldably held in place by a blow-out spring 114 which surrounds
  • the blow-out spring 114 bears against the upper face of a spring retainer 120 also mounted in the chamber 110 in adjusting screw 48.
  • the lower stop 80 is yieldable, as the washer 82 may be moved downwardly against the bias of the spring 114 when engaged by the shoulder 112 as the spring 114 compresses.
  • the spring retainer 120 is described in further detail below in connection with the assembling and disassembling of the pipetter.
  • a second coil spring 122 surrounds the piston 50 and extends between the coupling 54 and a seat 126 formed in the lower end of the O-ring retainer 44 (see FIG. 5).
  • the spring 122 serves as the main spring to yieldably oppose downward actuation of the pipetter piston 53. Any downward motion of the pipetter piston 53 causes the coil spring 122 to compress (see FIG. 10), and whenever the actuating
  • a stripper assembly 150 is in part mounted within the body 20 and in part lies below the body and surrounds the tip holder 26.
  • the assembly 150 includes a stem 152 mounted for reciprocal motion within the housing 20 along a path parallel to the axis of the pipetter piston 53 (see FIG. 4).
  • the rod 152 carries an actuating button 154 at its upper end 156, which may be conveniently actuated by the thumb of the hand which grips the body 20, as is evident in FIG. 1.
  • the rod 152 is supported in an opening 158 in flange 160 formed as an integral part of the body 20.
  • a compression spring 162 surrounds the upper portion of the rod 152 between the flange 160 and the lower end 164 of actuating button 154 and urges the rod upwardly in the body.
  • the upward travel of the rod 152 is limited by the shoulder 166 formed on the rod and which engages the flange 160.
  • the downward travel of the rod 150 is limited by engagement of the flange 170 of the actuating button 154 with the edge 172 of the body 20.
  • the lower end 176 of the rod 152 has a threaded stem 178 of reduced diameter, which extends
  • the stripper 181 is tapered slightly in a downward direction so that its ' lower end 182 has an inner diameter which is only slightly greater than the outer diameter of the tip holder 26.
  • the inner diameter of the stripper 181 increases in an upward direction and is significantly enlarged at its upper end 184 so as to surround the locknut 32.
  • the strength of the stripper 181 at its upper end is enhanced by the rib 188 connected to the flange 179 at the location where the threaded stem 178 of the rod 152 extends through the flange.
  • the spindle 76 on the adjusting screw 48 varies the position of the upper stop 70 which limits the extent that the pipetter piston 53 may be retracted into the body 20. It is essential for accurate repeatability of the pipetter that any particular setting of the stop 70 be fixed when a series of liquid transfers is to be made by the device.
  • the spindle may be locked against rotation on the adjusting screw by means of the lock slide 200 (see FIGS. 4, 6 and 7).
  • the lock slide 200 mounted in body 20 is moved trans1ationally in the body by means of a finger grip 202 mounted in slot 204 in the body wall 206.
  • the finger grip has an upwardly extending arm 208 which extends along the outer surface of the
  • the spindle 76 overlaps the upper end 210 of the arm 208 even in the uppermost position of the spindle as shown in FIG. 4.
  • a ramp 212 is formed on the outer surface of the adjusting screw 48, which cooperates with a cam surface 214 on the inner face of the upper end 210 of arm 208.
  • the cam surface 214 rides upwardly on the ramp 212 causing the end 210 of the arm to bear against and bind on the inner surface of the spindle 76.
  • the friction contact between the arm 208 and the inner surface of the spindle prevents the spindle from rotating and, therefore, the stop 70 remains in a fixed position to insure accurate, repetitive metering of the fluid. The spindle will not be moved again until the lock slide 200 is released.
  • the adjusting screw 48 is split so as to divide its lower end into two finger-like portions 219. Each finger portion carries an ear 220 on its outer surface that is sized to register with the openings 222 in the body wall 206.
  • the finger portions 219 are squeezed together as in FIG. 9 so that they allow the ears 220 to clear the lower edge 223 of the body and snap into the openings 222.
  • the spring retainer 120 is inserted into the adjusting screw to prevent its finger portions 219 from collapsing toward one another and dislodging the ears 220 from the openings 222.
  • the lower end of the retainer 120 is tapered slightly, which causes the retainer to wedge in place within the screw 48 and rigidly support the barrel shape of screw. To disassemble the unit, the retainer 120 may be pulled from the adjusting screw, and the adjusting screw
  • the retainer 120 as described above, also locks in the blow-out spring 114 that biases the piston 53 upwardly in the assembly.
  • the retainer 120 may have projections 1201 at its upper end that register with slots in the adjusting screw to form a bayonet connection between the two, to prevent the retainer from accidentally falling from the assembly (see FIGS. 4 and 12) .
  • a portion of the spindle 76 is clearly visible through the window 100 in the body.
  • the portion 102 viewable through the window carries a vernier scale which cooperates with the linear scale 104 on the window 100 so as to subdivide each unit of the linear scale into ten parts.
  • the units are a function of the pitch of the adjusting screw and spindle, the diameter of the pipetter piston 53, etc.
  • the pipetter of the present invention operates as follows:
  • a reservoir of fluid to be transferred and a plurality of wells to receive the transferred liquid are provided, and a selected volume to be transferred from the reservoir to each of the wells is known.
  • the operator rotates the exposed portion of the spindle so as to set it to the selected position on the scale visible through the window.
  • a rack of disposable pipetter tips T is provided which enables the tip holder 26 to be inserted in the appropriate end of the disposable tip and frictionally engage it.
  • the operator With the tip in place on the holder 26, and the setting having been made with the spindle to position the stop 70, the operator moves the lock slide 200 upwardly with the finger grip 202 so that the upper end 210 of the arm 206 engages the inside of the spindle to hold it firmly against further rotation.
  • the operator then depresses the button 58 carried by the spindle piston 53 until the shoulder 112 engages the stop
  • the pipetter is next positioned with the tip T aligned with the open end of the well W to which the liquid is to be transferred, and the operator again depresses the button 58 causing the pipetter piston to move downwardly.
  • the pressure in the passage 60 in the tip holder and the passage 61 in the tip T causes the liquid in tip T to be discharged into the well. Essentially complete discharge occurs when the shoulder 112 again engages
  • the operator need only press the button 154 and move the rod 152 against the bias of spring 162 until the flange 170 of the button engages the end 172 of the body 20. This action will cause the stripper 181 to move downwardly on the tip holder and cause the lower end 182 of the
  • the pipetter of the present invention may very quickly and easily be adjusted to vary the volume of liquid drawn into the device upon actuation of the button 58.
  • the adjustment is made simply by turning the spindle which is exposed through the window 100.
  • the top stop 70 may be positioned so as to cause the pipetter piston to draw in the desired volume of liquid when the pipetter piston is depressed and then released.
  • the vernier scale visible in the window enables the user to quickly set the stop to provide the desired metering.
  • the spindle is very easily turned, it may quickly and firmly be locked in any set position by means of the lock slide 200. By moving the slide upwardly toward the top end of the body in the slot provided
  • the arm 208 will wedge underneath the lower end of the spindle 76 and prevent it from rotating and changing the position of stop 70. This will ensure accurate repeatability of the device as it is used over and over again to transfer like quantities of liquid.
  • the lock is very effective and easy to operate, it may be readily disengaged merely by sliding the lock slide downwardly by means of the finger grip 202 so that the arm 208 releases the spindle.
  • the spindle may then very easily and quickly be spun through a substantial number of turns to make a gross adjustment.
  • Another advantage of the " present invention is the elimination in the device of screw machined parts. All but a few of the parts of the device may be injection molded of a suitable plastic material. This mode of construction is reflected in reduced manufacturing costs for the device. Manufacturing costs are also minimized by the presence of only a few ultrasonically welded parts. In the construction of the pipetter described, only two ultrasonic welds are included, namely, at the attachment of the button 58 to the pipetter shaft 53 and at the connection of the stop 70 to the spindle 76.
  • the stripper may be removed from the assembly so as to expose the full length of the tip holder 26 and the coupling which joins the tip holder to the adjusting screw 48 and body 20. More particularly, removal of the stripper exposes the locknut 32 which may then be turned so as to free the flange 36 of the tip holder. And with the tip holder removed, the retainer nut 44 may be removed, which will allow the piston 50 to be withdrawn. This is particularly convenient for the user who may wish to autoclave the piston 50 if, for example, fluid has accidentally entered the passage 60 in the tip holder from the passage 61 in the tip T.
  • blow-out spring retainer 120 By removing the blow-out spring retainer 120 from the adjusting screw 48 and the plunger button 58 from the stem 56 of the guide rod 52, the blow-out spring 114, washer 82 and guide rod 52 may also be withdrawn from the body 20 through its lower end.
  • the device may be further disassembled by

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

Pipette portative actionnée manuellement, ayant un volume variable régulé par une tige rotative (76) et une vis de réglage (48), lesquelles régulent la position d'une butée (70), laquelle limite l'extrémité supérieure de la course du piston (53) de la pipette. L'extrémité inférieure de la course est régulée par une butée (80) mobile, poussée jusqu'à une première position par un ressort (114) de souffle, lequel peut être maîtrisé par une force supplémentaire appliquée au piston (53) de la pipette lorsque le contact se produit avec la butée (80) inférieure. Ladite pipette comprend également un éjecteur (181), lequel entoure le support (26) de la pointe et lequel est actionné par une tige (152) mobile dans le corps (20) de la pipette, le long d'un chemin parallèle au chemin du piston (53) de la pipette. Le piston (53) de la pipette et la tige (181) de l'éjecteur sont commandés par des actuateurs (58, 154) séparés, permettant un contact facile avec le pouce de la main maintenant le corps (20) du dispositif.
PCT/US1990/005800 1989-10-20 1990-10-10 Pipette Ceased WO1991005609A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
DE69008540T DE69008540T2 (de) 1989-10-20 1990-10-10 Pipette.
EP90915595A EP0496784B1 (fr) 1989-10-20 1990-10-10 Pipette

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US42440889A 1989-10-20 1989-10-20
US424,408 1989-10-20

Publications (1)

Publication Number Publication Date
WO1991005609A1 true WO1991005609A1 (fr) 1991-05-02

Family

ID=23682526

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1990/005800 Ceased WO1991005609A1 (fr) 1989-10-20 1990-10-10 Pipette

Country Status (4)

Country Link
EP (1) EP0496784B1 (fr)
JP (1) JPH05501222A (fr)
DE (1) DE69008540T2 (fr)
WO (1) WO1991005609A1 (fr)

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5435355A (en) * 1992-10-09 1995-07-25 Xiao; Yong Conical floating type fuel filling device
EP0704242A3 (fr) * 1994-09-30 1997-09-10 Ralf Ritter Pipette de distribution à répétition
US5983733A (en) * 1996-11-15 1999-11-16 Hamilton Company Manual pipette
EP1197264A3 (fr) * 2000-10-11 2002-11-27 Thermo Labsystems Oy Pipette mit einstellbarem Inhalt
FR2826881A1 (fr) * 2001-07-06 2003-01-10 Biohit Oyj Dispositif de pipette a bout jetable
EP1287326A4 (fr) * 2000-02-17 2003-03-05 Rainin Instr Llc Pipette manuelle a volume ajustable a reglage automatique du volume
US6923938B2 (en) 2001-10-16 2005-08-02 Matrix Technologies Corporation Hand-held pipettor
EP1559480A1 (fr) * 2004-01-21 2005-08-03 Eppendorf Ag Pipette avec mécanisme de déplacement fixé de façon détachable au mécanisme d'actionnement
WO2006103315A1 (fr) * 2005-04-01 2006-10-05 Thermo Electron Oy Pipette etalonnable
EP1759768A1 (fr) * 2005-08-31 2007-03-07 Eppendorf Ag Pipette
US7284454B2 (en) 2004-05-27 2007-10-23 Matrix Technologies Corporation Hand held pipette
EP3112026A1 (fr) * 2015-06-30 2017-01-04 Sartorius Biohit Liquid Handling Oy Pipette avec un mécanisme de retrait de pointe, procédé pour retirer une pointe et procédé de pipettage
FR3128889A1 (fr) * 2021-11-09 2023-05-12 Gilson Sas Dispositif de prelevement a deplacement positif, conçu ameliorer la prehension du piston d’un systeme piston-capillaire

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3473409B2 (ja) * 1998-06-11 2003-12-02 松下電器産業株式会社 分注装置
JP5657861B2 (ja) * 2008-12-30 2015-01-21 光陽化成有限会社 マイクロピペット装置
EP2826722B1 (fr) 2013-07-19 2016-06-29 Mettler-Toledo GmbH Appareil de dosage manuel et procédé de dosage pour des poudres ou des pâtes
JP6354844B2 (ja) * 2014-07-01 2018-07-11 株式会社安川電機 液体移送システム及び液体移送方法
JP7315625B2 (ja) * 2020-07-14 2023-07-26 エッペンドルフ エスイー 一体型プランジャを備えたピペット・チップとともに使用するためのピペット

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US4054062A (en) * 1976-05-03 1977-10-18 Oxford Laboratories Inc. Hand-held micropipettor with improved accuracy of liquid volumes transferred
US4151750A (en) * 1977-02-17 1979-05-01 Kommandiittiyhtio Finnpipette Osmo A. Suovaniemi Device for detaching and removing a disposable tip of a pipette
US4283950A (en) * 1978-08-04 1981-08-18 Kommandiittiyhtio Finnpipette Osmo A. Suovaniemi Device for detaching and removing a disposable tip of a pipette
US4909991A (en) * 1987-09-18 1990-03-20 Nichiryo Co., Ltd. Variable pipette

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AU6276773A (en) * 1972-11-21 1975-05-22 Excalibur Lab Pty Ltd Pipette
FI57543C (fi) * 1978-06-02 1980-09-10 Suovaniemi Finnpipette Volymreglerbar pipett

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Publication number Priority date Publication date Assignee Title
US4054062A (en) * 1976-05-03 1977-10-18 Oxford Laboratories Inc. Hand-held micropipettor with improved accuracy of liquid volumes transferred
US4151750A (en) * 1977-02-17 1979-05-01 Kommandiittiyhtio Finnpipette Osmo A. Suovaniemi Device for detaching and removing a disposable tip of a pipette
US4283950A (en) * 1978-08-04 1981-08-18 Kommandiittiyhtio Finnpipette Osmo A. Suovaniemi Device for detaching and removing a disposable tip of a pipette
US4909991A (en) * 1987-09-18 1990-03-20 Nichiryo Co., Ltd. Variable pipette

Non-Patent Citations (1)

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Title
See also references of EP0496784A4 *

Cited By (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5435355A (en) * 1992-10-09 1995-07-25 Xiao; Yong Conical floating type fuel filling device
EP0704242A3 (fr) * 1994-09-30 1997-09-10 Ralf Ritter Pipette de distribution à répétition
US5983733A (en) * 1996-11-15 1999-11-16 Hamilton Company Manual pipette
EP1287326A4 (fr) * 2000-02-17 2003-03-05 Rainin Instr Llc Pipette manuelle a volume ajustable a reglage automatique du volume
US7105130B2 (en) 2000-10-11 2006-09-12 Thermo Electron Oy Adjustable pipette
EP1197264A3 (fr) * 2000-10-11 2002-11-27 Thermo Labsystems Oy Pipette mit einstellbarem Inhalt
FR2826881A1 (fr) * 2001-07-06 2003-01-10 Biohit Oyj Dispositif de pipette a bout jetable
US6923938B2 (en) 2001-10-16 2005-08-02 Matrix Technologies Corporation Hand-held pipettor
US8088342B2 (en) 2001-10-16 2012-01-03 Matrix Technologies Corporation Hand-held pipettor
EP1559480A1 (fr) * 2004-01-21 2005-08-03 Eppendorf Ag Pipette avec mécanisme de déplacement fixé de façon détachable au mécanisme d'actionnement
US7284454B2 (en) 2004-05-27 2007-10-23 Matrix Technologies Corporation Hand held pipette
WO2006103315A1 (fr) * 2005-04-01 2006-10-05 Thermo Electron Oy Pipette etalonnable
US8631682B2 (en) 2005-04-01 2014-01-21 Thermo Fisher Scientific Oy Calibratable pipette
EP1759768A1 (fr) * 2005-08-31 2007-03-07 Eppendorf Ag Pipette
US7674432B2 (en) 2005-08-31 2010-03-09 Eppendorf Ag Pipetting device
EP3112026A1 (fr) * 2015-06-30 2017-01-04 Sartorius Biohit Liquid Handling Oy Pipette avec un mécanisme de retrait de pointe, procédé pour retirer une pointe et procédé de pipettage
CN106391155A (zh) * 2015-06-30 2017-02-15 赛多利斯百得液体处理公司 具有吸头移除机构的移液枪,以及移除吸头和移液的方法
US10239055B2 (en) 2015-06-30 2019-03-26 Sartorius Biohit Liquid Handling Oy Pipette with a tip removal mechanism, a method for removing a tip, and a method for pipetting
CN106391155B (zh) * 2015-06-30 2020-04-21 赛多利斯百得液体处理公司 具有吸头移除机构的移液枪,以及移除吸头和移液的方法
FR3128889A1 (fr) * 2021-11-09 2023-05-12 Gilson Sas Dispositif de prelevement a deplacement positif, conçu ameliorer la prehension du piston d’un systeme piston-capillaire
WO2023084175A1 (fr) * 2021-11-09 2023-05-19 Gilson Sas Dispositif de prélèvement à déplacement positif, conçu améliorer la préhension du piston d'un système piston-capillaire

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Publication number Publication date
DE69008540T2 (de) 1994-12-15
EP0496784A1 (fr) 1992-08-05
JPH05501222A (ja) 1993-03-11
EP0496784B1 (fr) 1994-04-27
EP0496784A4 (en) 1992-09-09
DE69008540D1 (en) 1994-06-01

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