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EP3784399B1 - Pipette à utiliser avec une pointe de pipette et une famille de pipettes comprenant plusieurs pipettes avec des volumes nominaux différents - Google Patents

Pipette à utiliser avec une pointe de pipette et une famille de pipettes comprenant plusieurs pipettes avec des volumes nominaux différents Download PDF

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Publication number
EP3784399B1
EP3784399B1 EP19718400.5A EP19718400A EP3784399B1 EP 3784399 B1 EP3784399 B1 EP 3784399B1 EP 19718400 A EP19718400 A EP 19718400A EP 3784399 B1 EP3784399 B1 EP 3784399B1
Authority
EP
European Patent Office
Prior art keywords
pipette
pusher
pipette tip
tip
housing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP19718400.5A
Other languages
German (de)
English (en)
Other versions
EP3784399A1 (fr
Inventor
Burkhardt Reichmuth
Uwe Dunker
Jens Wilmer
Tobias David
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf SE filed Critical Eppendorf SE
Publication of EP3784399A1 publication Critical patent/EP3784399A1/fr
Application granted granted Critical
Publication of EP3784399B1 publication Critical patent/EP3784399B1/fr
Active legal-status Critical Current
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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0224—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type having mechanical means to set stroke length, e.g. movable stops
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0237—Details of electronic control, e.g. relating to user interface
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/143—Quality control, feedback systems
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons

Definitions

  • the invention relates to a pipette for use with a pipette tip. Furthermore, the invention relates to a pipette tip family comprising several pipette tips of different types for use with a pipette.
  • Pipettes are used primarily in scientific and industrial laboratories with medical, molecular biology, and pharmaceutical applications for dosing selected volumes of liquids.
  • These liquids can be homogeneous (single-phase) liquids consisting of a single liquid component or a homogeneous mixture of several liquid components (solutions).
  • the liquids can be heterogeneous (multiphase) mixtures of a liquid with another liquid (emulsions) or a solid (suspensions).
  • Pipettes have a stem-shaped pipette body with a stud (attachment) at the lower end for clamping a pipette tip.
  • the stud is often conical, cylindrical, or partially conical and cylindrical and is also referred to as a "working cone.”
  • a pipette tip is a hollow tube with a tip opening at the lower end and a plug-in opening at the upper end, with which the pipette tip can be clamped onto the stud.
  • the liquid is drawn into and dispensed from the pipette tip.
  • the aspiration and dispensing of the liquid is controlled by the pipette.
  • Fixed-volume pipettes are used for pipetting constant volumes. With variable-volume pipettes, the volume to be dispensed is adjustable.
  • a mechanical counter is used to display the set volume.
  • the stroke of the drive mechanism is adjustable using an adjustment device coupled to the counter.
  • the pipette tip is After use, the tip is removed from the tube and can be replaced with a fresh pipette tip. This prevents contamination during subsequent pipetting operations.
  • Air-cushion pipettes have a piston-cylinder system in the pipette body, which is connected via a channel to a hole in the stem.
  • Pipette tips for air-cushion pipettes do not have an integrated piston. Moving the piston in the cylinder moves an air cushion to draw liquid into and expel it from a pipette tip clamped onto the stem.
  • Disadvantages of air-cushion pipettes include dispensing errors due to changes in the length of the air cushion caused by the weight of the aspirated liquid and the influences of temperature, air pressure, and humidity. Contamination of the pipette by aerosols can also be problematic.
  • Positive displacement pipettes are used with pipette tips with an integrated piston (positive displacement pipette tips).
  • This type of pipette features a stud for attaching the pipette tip and a drive mechanism that can be coupled to the integrated piston to move the piston.
  • the piston comes into direct contact with the liquid, eliminating the effects of an air cushion.
  • Positive displacement pipettes are particularly suitable for dispensing liquids with high vapor pressure, high viscosity, or high density, and for applications in molecular biology where aerosol-free operation is critical to avoid contamination.
  • Air cushion or positive displacement pipette tips for single use or reusable are made of plastic or glass.
  • Pipettes are available in different sizes, each with a matching pipette tip type or several matching pipette tip types with similar nominal volumes. For different applications The user must maintain pipettes and pipette tips of different sizes, which incurs corresponding costs for acquisition, maintenance, and storage. Color-coding pipettes and pipette tips is a common practice to facilitate correct identification. This poses a risk of misinterpretation by the user.
  • the EP 2 574 402 B1 describes a syringe for use with a dosing device that has a code on the upper edge of the syringe barrel that is dependent on the type of syringe. Furthermore, a dosing device for use with a syringe is described that has a scanning device with a ring sensor for scanning the code, which is connected to an evaluation device that, in the event of error-free scanning, controls the dosing device to an operating state dependent on the code. This includes, for example, the display of the set dosing volume.
  • the DE 199 48 818 A1 Describes a syringe with a code on the top of the syringe plunger and a pipette ("syringe holder") with an information reader for reading the code.
  • a specific syringe of a certain type e.g., a 50 ml syringe
  • this value can be output via optical display elements (e.g., LEDs or LCDs).
  • the WO 2011/110141 A1 , SU 597 411 A1 and US 4 679 446 A describe air-cushion pipettes with stems divided into several conical sections with steps in between, whereby the different conical sections have different clamping zones for clamping pipette tips of different sizes.
  • Several channels extend through the stem, one of which opens into the central lower end face of the stem and the others at the undersides of the steps. The other ends of the channels are connected to piston-cylinder systems. Due to the multiple channels in the stem and the multiple piston-cylinder systems, these air-cushion pipettes are structurally complex. Clamping pipette tips of different sizes requires different clamping forces due to the different clamping zones.
  • the EP 0 152 120 A2 Describes an air-cushion pipette comprising a digital linear drive module and connectable displacement modules of various sizes with conical pins for clamping pipette tips.
  • a pipette tip of a specific size can be clamped onto the pins of each displacement module.
  • the displacement module must be replaced with the displacement module that matches the pipette tip size.
  • the US 2002/0001545 A1 and the US 6,749,812 B2 describe an air-cushion pipette with an automatic tip ejector, which has a spring-loaded hub for attaching pipette tips and a spring-loaded ejector sleeve guided thereon. Furthermore, tip detection for pipette tips is provided, with different tip types having different base configurations. With this tip detection, different pipette tip types are recognized by a different displacement of the hub relative to the ejector sleeve, which is detected by an electronic sensor.
  • the disadvantage of conventional pipettes is that the detection of pipette tips or syringes is carried out using electronic sensors. Both the displacement of the tip and the displacement of the ejector sleeve are detected by electronic sensors.
  • the electronic sensors can be subjected to strong stresses from forces, vapors, etc. and are prone to failure.
  • the electronics increase the construction effort and the equipment costs. Furthermore, the electronics limit the possibilities for To sterilize pipettes. Different pipette tip types have different sized clamping surfaces on the tip.
  • the invention aims to create a pipette that is less prone to failure and can be used with pipette tips of different types. Furthermore, a pipette tip family comprising several pipette tips of different types that can be used with the same, less prone to failure pipette is to be created.
  • the pipette according to the invention is suitable for use with a pipette tip from a pipette tip family.
  • Pipette tips of different types can be clamped onto the stem of the same pipette. These form a pipette tip family.
  • Pipette tips of different types differ in particular with regard to one or more of the following features: nominal volume, shape, dimensions, material (e.g. PP and/or PE), with or without coating, with or without surface treatment (e.g. treatment using pearl effect technology for better drainage of detergent-containing liquids), tubes with or without an inserted filter (filter tip), electrically insulating or electrically conductive, and degree of purity (e.g. standard, sterile, protein-free, PCR-pure, biologically pure).
  • Pipette tips of different types have collars of different heights. Pipette tips of the same type have collars of the same height.
  • the height of the collar is characteristic of the type of pipette tip.
  • the collar is the area at the upper end of the pipette tip with which the pipette tip protrudes from the stud or into which the stud dips when the pipette tip is clamped onto the stud.
  • the pipette tip has a seating area on the inner circumference of the collar for clamping onto the stud.
  • the sensing element is arranged next to the stud so that it can be displaced by the collar of a pipette tip when the tip is clamped onto the stud.
  • the sensing element can be displaced to varying degrees by collars of different heights. However, it can also be non-displaceable by a collar of low height and displaceable by one (or more) collars of different heights of greater height.
  • the The scanning device adjusts the display of the display device.
  • the display includes, for example, a volume display or a display of another feature of the pipette tip. The user can use pipette tips of different types and directly read a display dependent on the type used. The display is automatically adjusted to the type of pipette tip in use.
  • the mechanical display device is a display device in which the different displays are adjusted mechanically.
  • the display device is coupled to the scanning device to adjust the display.
  • the display is adjusted by moving the scanning element. Since the pipette does not require an electrical sensor to detect the pipette tip type, the risk of failure is reduced. Another advantage is that the pipette can be designed purely mechanically without electronic components, so there is no need to replace or recharge batteries and the sterilization options are expanded.
  • the display which is dependent on the type of pipette tip, comprises or is a volume display.
  • the pipette tips are of different types and have different nominal volumes.
  • the nominal volume is the volume of the pipette tip that can be used for pipetting. It is specified by the manufacturer and used to indicate the measuring range.
  • the height of the collar increases with the nominal volume of the pipette tip.
  • the height of the collar decreases with the nominal volume of the pipette tip.
  • the volume display comprises at least one volume displayed by the display device.
  • this is the volume that can be dosed with an attached pipette tip with a specific nominal volume and a specific setting of the setting device, and/or in the case of a variable or fixed-volume pipette, the nominal volume of the clamped-on pipette tip. As the nominal volume of the pipette tip increases, the volume display is shifted towards higher values.
  • the user can use pipette tips with different nominal volumes and read the set pipetting volume and/or nominal volume directly from the display without any interpretation effort.
  • the mechanical display device displays a display dependent on a specific pipette tip type if the sensing element is not displaced by the collar of a pipette tip attached to the pin. This is the case when no pipette tip is attached to the pin, or when a pipette tip with a short collar is attached to the pin, which is not high enough to displace the sensing element.
  • the display device is set to display a display dependent on the type of pipette tip with the short collar.
  • the sensing element can be held by a stop device in an initial position from which it is not displaced when a pipette tip with the short collar is clamped onto the pin.
  • the sensing device sets a display dependent on the type of pipette tip with the long collar.
  • the display of the indicator is always adjusted when a pipette tip with a collar is attached to the pin.
  • control element is coupled to the drive element.
  • control element is permanently connected to the drive element or coupled to it via a gear.
  • the pipette is an air-cushion pipette for use with an air-cushion pipette tip.
  • a displacement device is arranged in the pipette housing and is connected via a channel to a hole at the lower end of the pin.
  • the displacement device is a displacement chamber with a displaceable displacement element therein.
  • the displacement device is a cylinder with a displaceable piston sealingly guided therein.
  • the drive element is a lifting rod that can be displaced vertically in the pipette housing and is coupled at the lower end to the displacement element and at the upper end to the operating element.
  • the operating element is an operating button that protrudes from the upper end of the pipette housing and can be pressed at least partially into it.
  • the pipette is a positive displacement pipette for use with a positive displacement pipette tip.
  • the drive element is a lifting rod guided in a vertical through-channel of the pin, with a vertical bore at the lower end for clamping a piston rod protruding from the top of the integrated piston of a pipette tip.
  • the upper end of the lifting rod is coupled to the operating element.
  • the operating element is an operating button that protrudes from the upper end of the pipette housing and can be pressed at least partially into the pipette housing.
  • the pipette is a fixed-volume pipette.
  • the drive element can be moved back and forth by a defined measuring stroke.
  • the pipette can be used with positive-displacement pipette tips that accommodate different tube diameters and correspondingly adapted piston diameters. to pipette different volumes with the same measuring stroke.
  • the fixed-volume pipette has a display device that indicates the nominal volume of a pipette tip clamped onto the attachment.
  • the pipette is a variable pipette.
  • This pipette has an adjustable volume.
  • the measuring stroke of the pipette is adjustable.
  • the pipette has a display device that displays the set measuring stroke.
  • the display device comprises a counter.
  • the variable pipette comprises a first setting element for setting the volume, which is arranged at the upper end of the pipette housing.
  • the first setting element is simultaneously the operating element or an additional component.
  • the first setting element is, for example, a sleeve-shaped setting knob rotatably mounted at the upper end of the pipette housing, which surrounds an operating button, wherein the operating button can be pressed at least partially into the setting knob.
  • the variable pipette has a display device that shows the nominal volume of a pipette tip clamped onto the attachment.
  • the display device shows the nominal volume in addition to the set volume.
  • the pipette has a piston spring which, after the operating element is relieved, displaces the drive element upwards until a first stop device becomes effective (strikes). By actuating the operating element, the lifting rod can be displaced downwards against the action of the piston spring until a second stop device becomes effective (strikes).
  • the operating element is coupled to the drive element via a gear, which enables the drive element to be driven in different directions.
  • the drive device comprises a switching device such that, upon actuation of the operating element, the drive element can initially be displaced downwards until the second stop device becomes effective, and upon further actuation of the operating element, the switching device controls the displacement of the drive element in the opposite direction until the first stop device becomes effective.
  • the nominal volumes of the pipette tips or other characteristics characterizing the type of pipette tips that can be used with the pipette are indicated on the pipette and/or the pipette is provided with one or more markings indicating the nominal volumes or other characteristics characterizing the type of pipette tips that can be used with the pipette.
  • the nominal volumes or other characteristics characterizing the type are indicated on the pipette tips and/or the pipette tips are provided with one or more markings indicating the nominal volumes or other characteristics characterizing the type.
  • the pin is rigidly connected to the pipette housing, wherein the pin is preferably not movable relative to the pipette housing.
  • the pin can be directly or indirectly connected to the pipette housing.
  • the pin can be rigidly connected to a frame or chassis that supports the pipette housing and is rigidly connected thereto.
  • the scanning device comprises a slider which is guided vertically displaceably on the pipette housing and which has the scanning element at the lower end of the slider and whose upper end is coupled to the display device.
  • the lower end of the slider, and thus the slider itself can be moved upwards to varying degrees.
  • the upper end of the slider acts on the display device and adjusts the volume display or another display depending on the pipette tip type.
  • the slider has various nominal volume information or various other information characterizing the type of pipette tip on an outer side in a vertical direction
  • the pipette housing has a window in the displacement area of the nominal volume information or the other information on the slider, through which only a complete nominal volume information or a complete other information on the slider is visible from the outside.
  • the nominal volume information or other information and the window are arranged in such a way that for each pipette tip attached to the pin, the corresponding nominal volume information or other information is visible from the outside through the window. This is controlled by the height of the collar of the pipette tip. This makes it easy for the user to see which pipette tip type is attached.
  • the pipette comprises a first stop device with a first stop element on the drive device and a second stop element on the pipette housing, which can be brought into contact with one another by displacement of the drive element in a first direction, and a second stop device with a third stop element on the drive device and a fourth stop element on the pipette housing, which can be brought into contact with one another by displacement of the drive element in a second direction, which is opposite to the first direction, in order to define a measuring stroke of the drive device together with the first stop device.
  • the pipette comprises an adjustment device with a first adjustment element mounted on the pipette housing and a second adjustment element on the first or second stop device for adjusting the measuring stroke by adjusting the first adjustment element and a mechanical counter coupled to the adjustment device for displaying the setting of the adjustment device, wherein the scanning device is coupled to a mechanical range adjustment device on the counter, which is designed to set a counting range of the counter which depends on the position of the scanning element relative to the pipette housing.
  • the range adjustment device comprises a marking arranged externally in front of the counter at the upper end of the slide, whereby when pipette tips with collars of different heights are clamped onto the pin, the slide can be moved upwards to different distances in order to position the marking to indicate different counting ranges to the side next to or between different positions on the counter.
  • collars of different heights position the marking to the side next to or between different positions on the counter, i.e. to the side next to or behind the first place before the decimal point and/or to the side next to or in front of the first place after the decimal point.
  • the marking can be adjusted so that the counter directly indicates the volume that can be pipetted with the attached pipette tip for the given measuring stroke setting. This allows the counting range of the counter to be shifted by one or more powers of ten. This enables pipette tips whose nominal volumes differ by one or more powers of ten to be used with the same pipette.
  • the range setting device comprises a switchable gear, via which the counter is coupled to the setting device.
  • the switchable gear By switching the switchable gear, various Gear ratios are selectable.
  • the gear can be switched so that the counter indicates the volume that can be pipetted with the attached pipette tip at the given stroke setting.
  • the sensing element is formed at the lower slide end of a slide sleeve, which is vertically movable along a housing shaft of the pipette housing, which has a pin at its lower end.
  • the slide sleeve is guided particularly easily and precisely along the housing shaft.
  • the marking is a boundary of a diaphragm connected to the upper end of the slide. Digits of the counter are readable from the outside through the diaphragm and a window in the pipette housing. According to a further embodiment, the diaphragm is adjusted by the slide so that only the digits that indicate the set pipetting volume for the attached pipette tip are readable and/or framed by the diaphragm, and/or the marking indicates the first digit before the decimal point and/or the highest digit after the decimal point. According to a further embodiment, the digits of the counter or the counter rollers are arranged one above the other.
  • the digits of the counter or the counter rollers are arranged next to one another in a horizontal direction.
  • the marking is connected to the upper end of the slide via a gear, which converts a vertical displacement of the upper end of the slide into a horizontal displacement of the marking.
  • the marking is a bar-like graduation mark in a diaphragm frame of the diaphragm, which can be positioned behind the smallest pre-decimal place of the counter and/or before the highest decimal place of the counter, which applies to a clamped-on pipette tip.
  • the pipette comprises a first spring device with a first engagement point on the pipette housing and a second engagement point on the drive device in order to displace the drive element in the first direction until the first stop element rests on the second stop element when the operating element is relieved.
  • the first spring device is a first coil spring, which is supported at its lower end by a first spring bearing on the housing and at its upper end by a second spring bearing on the lifting rod.
  • the lifting rod is guided through the first coil spring.
  • the operating element is also the first adjustment element, wherein the operating element is rotatably mounted in the housing and coupled to the second adjustment element via a first gear.
  • the first gear comprises a spindle nut, a threaded spindle which is threadably engaged with the spindle nut, vertically aligned, and rotatably coupled to the operating element, having a longitudinal bore through which the lifting rod is passed, and a linear guide which is aligned vertically in the pipette housing and on which the spindle nut is guided.
  • the spindle nut is the second adjustment element
  • the lower end face of the spindle nut is the first stop element
  • a bead protruding radially from the lifting rod below the spindle nut is the second stop element.
  • the mechanical counter is coupled via a second gear to the setting knob or operating knob rotatably mounted in the housing.
  • a further embodiment has a third stop device with a fifth stop element on the slide and a sixth stop element on the Pipette housing and a second spring device with a third engagement point on the pipette housing and a fourth engagement point on the slider to apply downward pressure to the slider and to position the fifth stop element against the sixth stop element when no pipette tip is attached to the pin. This holds the slider in a defined lower stop position before a pipette tip is attached, from which the slider can be moved upward by attaching the pipette tip to the pin.
  • the second spring device is a second coil spring, which is held at the lower end by a third spring bearing on the slide and at the upper end by a fourth spring bearing on the housing.
  • This embodiment is structurally simple and space-saving.
  • the lifting rod can be moved downwards by pressing the control button until the second stop device becomes active. This can be done immediately before the liquid is drawn into the pipette tip.
  • the user simply needs to release the control button, as the first spring mechanism moves the lifting rod upwards. Pressing the control button again dispenses the liquid from the pipette tip.
  • the pipette tip can be manually detached from the pipette.
  • the ejection of the pipette tip with integrated piston by the operating element is made possible by the lifting rod having a lower lifting rod part, the vertical bore extending to the upper end of the lower lifting rod part, an upper lifting rod part engaging in the vertical bore and a third spring device being integrated into the upper lifting rod part, which is designed such that by pressing the operating element downwards, the upper lifting rod part can initially be displaced downwards in parallel with the lower lifting rod part until the third stop element rests against the fourth stop element and when an increased pressure force is applied to the operating button, the third spring device compresses so that the upper lifting rod part penetrates deeper into the lower lifting rod part, whereby the piston rod is pushed out of the vertical bore and the pipette tip is pushed off the pin.
  • the air cushion pipette has an ejector which enables the pipette tip to be released by actuating the operating element and/or an additional ejector button.
  • the slider is connected at the top to an ejector button protruding from the upper end of the pipette housing.
  • the slider can be moved downwards to allow the lower end of the slider to strip a pipette tip from the stem.
  • the slider thus forms an ejector, allowing pipette tips to be detached from the stem without touching them with the hand.
  • the withdrawal of the piston from the pipette tip can be limited by a stop so that the piston is separated from the lifting rod by stripping off the pipette tips.
  • a further embodiment comprises a fourth stop device with a seventh stop element on the release button and an eighth stop element on the housing, which are designed to limit the upward displacement of the release button and a transmission device between the release button and the slider, which is designed not to displace the release button upwards when the slider is displaced upwards and to displace the slider downwards when the release button is displaced downwards.
  • the starting position of the non-actuated release button is not changed by the upward displacement of the slider.
  • the release button takes up Attaching pipette tips with collars of different heights does not result in different starting positions.
  • the path of the ejector button for removing a pipette tip from the stem can be kept constant regardless of the pipette tip being used. Compared to designs in which the ejector button and slider are rigidly connected, this improves ease of use and the overall appearance.
  • the transmission device is a fourth spring device having a fifth engagement point on the release button and a sixth engagement point on the slide, whereby upon upward displacement of the slide, the fourth spring device is compressed, and upon downward displacement of the release button, the fourth spring device is first compressed and then the release button carries the slide, or
  • the transmission device is a locking device arranged between the release button and the slide, which enables upward displacement of the slide relative to the release button and prevents downward displacement of the push button relative to the slide.
  • the locking device is a directional locking mechanism.
  • the upward displacement of the release button is limited by the fourth stop device.
  • the fourth stop device assumes a defined upper stop position from which it can be moved downward.
  • the release button and release rod are not rigidly coupled to each other, but via a fourth spring device.
  • the fourth spring device can in particular be a (helical) spring or a soft elastic element (e.g. an element made of rubber, silicone or a thermoplastic elastomer).
  • the release button and slider are only a short distance apart, so that when the release button is actuated, the fourth spring device is compressed first, and when the release button rests against the slider, the latter is compressed with is displaced downwards.
  • an upward movement of the slider when attaching a pipette tip does not lead to an upward movement of the ejection button, since the fourth stop device prevents this and only the fourth spring device is compressed.
  • the ejector button and the slider are connected to each other via a locking device.
  • the locking device has the function of preventing the movement of a component in a certain direction, while allowing movement in the opposite direction.
  • the locking device comprises, for example, a gear wheel mounted on the ejector button, which has a freewheel.
  • the slider is provided with teeth on one side like a rack. When a pipette tip is attached, the ejector is moved upwards and the gear wheel rotates freely. When the ejector button is pressed, however, the free rotation of the gear wheel is stopped and the slider is moved downwards so that the lower end of the slider wipes the pipette tip off the pin.
  • Another embodiment in addition to the slide, has an ejection slide which is guided vertically on the pipette housing and which is connected at the upper end to the ejection button and whose lower end is arranged next to the pin, offset radially to the lower end of the slide, wherein (i) the lower ends of the slide and ejection slide are arranged at the same height, so that by clamping a pipette tip with two circumferential steps at the upper end onto the pin, the slide can be displaced by the inner step and the ejection slide cannot be displaced by the outer step, and by actuating the ejection button, the lower end of the ejection slide can be pressed against the outer step of the pipette tip in order to strip it off the pin, or (ii) the lower end of the slide is arranged lower than the lower end of the ejection slide, so that by clamping a pipette tip with a flat upper edge onto the pin, the slide can be displaced upwards and the ejection slide cannot can be moved upwards and by
  • the range setting and ejection of the pipette tip are achieved by an ejection slide that is separate from the slide and one from it.
  • This can, for example, be an outer and an inner slide sleeve, with the inner slide sleeve being guided on a shaft of the pipette and the outer slide sleeve on the inner slide sleeve. While one slide sleeve is moved upwards when the pipette tip is attached and shifts the range setting, the other slide sleeve is not moved. This slide sleeve is only used when the ejection button is pressed.
  • this is achieved by the pipette tip having two concentric, circumferential steps at the upper end, with the step that projects further upwards actuating the range setting and the lower end of the ejection slide meeting the step that projects less upwards.
  • this is achieved by the slider projecting further downwards than the ejector slider when not actuated, so that by attaching a pipette tip with a flat upper edge, the slider and not the ejector slider is displaced.
  • the neck has a circumferential shoulder at a distance from its lower end on the outer circumference, so that a pipette tip can be clamped onto the neck with an upper edge of the collar or a circumferential inner step projecting radially inwards on the inner circumference of the collar until the inner step rests against the shoulder. This ensures that the neck always engages the clamped-on pipette tip of the respective type to the same depth. If the collar of the pipette tip has an expanded section above the inner step and the neck above the shoulder is not expanded correspondingly to the collar, the pipette tip can only be clamped with A seating area below the inner step allows the pipette tip to be clamped onto the hub.
  • the pipette is a single-channel pipette.
  • a single-channel pipette has only a single attachment at the lower end of the housing for clamping on a pipette tip.
  • the pipette is a multi-channel pipette.
  • a multi-channel pipette is a pipette that has several parallel attachments at the lower end of the pipette housing for simultaneously clamping on several pipette tips.
  • the scanning device can, in particular, have a scanning element arranged next to only one or next to several pins.
  • the pipette tip family according to the invention for use with a pipette comprises at least two pipette tips of different types, each pipette tip having a tip opening at the lower end, a collar having a plug-on opening with a height characterising the type of pipette tip at the upper end, and on the inner circumference of the collar has a seating area for clamping onto a pin with a specific contour of a pipette, whereby pipette tips of different types can be clamped with their seating area onto the same clamping zone of the pin, so that they stand with their collar at different heights on the pin.
  • the collar is the area at the top of the pipette tip with which this pipette tip protrudes from the stem or into which the stem dips when the pipette tip is clamped onto the stem.
  • the height of the collar can be measured starting from the seating area of the pipette tip, which is formed, for example, by a circumferential bead on the inner circumference of the collar.
  • Pipette tips of different types can be clamped with their seating areas onto the same clamping zone of the stem, so that the distance of the top end of the collar from the seating area increases proportionally to the immersion depth of the stem in the collar.
  • pipette tips with collars of different heights control a sensing element of a sensing device on the pipette such that the sensing device sets a display on the pipette display device that depends on the pipette tip type. Consequently, when pipette tips of different types are clamped onto the attachment, the display is set to indicate the type of pipette tip currently attached.
  • the pipette tips in the pipette tip family can be air-cushion pipette tips or positive displacement pipette tips. Positive displacement pipette tips feature a cylindrical piston track between the tip opening and the tip opening, as well as an integrated piston that can be moved along the piston track.
  • Pipette tips of different types differ in particular with regard to one or more of the following characteristics: nominal volume, shape, dimensions, material (e.g. PP and/or PE), with or without coating, with or without surface treatment (e.g. treatment using pearl effect technology for better drainage of detergent-containing liquids), tubes without inserted filter or with inserted filter (filter tip), electrically insulating or electrically conductive, degree of purity (e.g. standard, sterile, protein-free, PCR-pure, biologically pure).
  • nominal volume, shape, dimensions, material e.g. PP and/or PE
  • coating with or without surface treatment (e.g. treatment using pearl effect technology for better drainage of detergent-containing liquids)
  • surface treatment e.g. treatment using pearl effect technology for better drainage of detergent-containing liquids
  • electrically insulating or electrically conductive degree of purity (e.g. standard, sterile, protein-free, PCR-pure, biologically pure).
  • the pipette tip family comprises at least two pipette tips with different nominal volumes, wherein the pipette tips with different nominal volumes can be clamped onto the same pin with their seating area, so that their collars protrude at different heights from the pin.
  • pipette tips with collars of different heights control a sensing element of a sensing device of the pipette such that the sensing device sets or maintains the volume display of a display device of the pipette depending on the height of the collar. Consequently, when pipette tips with different nominal volumes are clamped onto the attachment, the corresponding volume display is always displayed.
  • the pipette tip family includes pipette tips whose nominal volumes differ by one or more orders of magnitude. These pipette tips are usable with a pipette with a mechanical counter to set a mark on the sensing device next to one of the counter rollers.
  • the heights of the collars differ by a multiple or an integer multiple of the height of a counter roller.
  • pipette tips of different types can be clamped onto the same clamping zone of the same pin, it is possible to clamp pipettes of different types onto the pin with the same clamping force. This is advantageous for equally secure clamping of pipette tips of different types onto the pin.
  • the clamping zone is a continuous, step-free zone of the pin that runs around the pin.
  • pipette tips of different types are designed so that they can only be clamped onto the same clamping zone of the pin. With this embodiment, pipette tips of different types cannot be clamped onto zones other than the clamping zone of the pin, thereby achieving identical clamping forces. With air-cushion pipette tips, this can achieve a tight fit on the pin with identical clamping forces.
  • the seating area of the pipette tip contains a circumferential bead and/or a circumferential groove.
  • a bead of the seating area of a pipette tip engages in a groove of the clamping area on the pin of the pipette and/or a bead of the clamping area engages in a groove of the seating area.
  • the height difference between the collars is a multiple or an integer multiple of the height of a counter roller, which is in the range of 1 to 7 mm, in particular in the range of 3 to 5 mm, and preferably 4.5 mm. In these ranges, the digits of the counter wheels are clearly visible while maintaining a compact design.
  • the pipette tips of the pipette tip family according to the invention have, in addition to the plug-in opening on the inner circumference, a seating area with which they can be clamped onto the pin of a pipette.
  • the seating area is adapted to the contour of at least part of the pin.
  • the seating area can either have a contour complementary to the contour (e.g., a cylindrical or conical contour) of at least part of the pin or have protruding sealing and/or guiding structures (e.g., sealing and/or guiding beads) on its inner circumference that are adapted to the contour (e.g., a cylindrical or conical contour) of at least part of the pin.
  • the pipette tips with different nominal volumes are adapted with their seating area to the same pin. can be clamped on so that their collars stand at different heights on the pin.
  • the clamping onto the pipette attachment is determined by a ninth stop element, which limits the sliding of the pipette tip onto the pipette stem by resting on the pipette.
  • the ninth stop element is, for example, a radially inwardly projecting inner step of the pipette tip, which runs around the inner circumference of the pipette tip and rests against the lower end of the stem, or a downwardly tapered conical braking area of the pipette tip, which rests against the lower end of the stem.
  • the ninth stop element can be designed in the above manner for both an air cushion and a positive displacement pipette tip.
  • the ninth stop element is an upper side or a radially outwardly projecting shoulder of the piston or piston rod, which rests against the pipette's lifting rod.
  • the ninth stop element is an upper edge of the collar or a circumferential inner step of the pipette tip, projecting radially inward on the inner circumference of the collar, above which the collar has an expanded section, so that the pipette tip can be clamped onto a pipette attachment until the upper edge of the collar or the inner step rests against a seating area arranged below the upper edge of the collar or the inner step, until the upper edge of the collar or the inner step rests against a shoulder of the attachment.
  • This embodiment ensures that pipette tips of a certain type can be pushed onto an attachment at the same height.
  • the upper edge of the collar can control the sensing element of a sensing device, regardless of whether the upper edge of the collar or the inner step rests against the shoulder.
  • the pipette tips are designed such that the pipette tips can only be clamped onto a The seating area can be clamped onto the attachment below the upper edge of the collar or below the inner step, and the extended section of the collar above the inner step cannot be clamped onto the attachment.
  • the pipette tips are designed such that their seating area can be clamped onto a cylindrical or conical section of an attachment below a shoulder of the attachment.
  • the pipette tips are designed such that an extended section of the collar above the inner step cannot be clamped onto a cylindrical or conical section of the attachment above the shoulder.
  • the extended section of the collar above the inner step is designed such that a gap exists between the extended section of the collar and an extended section of the attachment above the shoulder when the pipette tip is clamped onto the attachment. This makes it possible for pipette tips of different types to be clamped onto the same attachment with equal firmness. This facilitates secure clamping onto the attachment and easy removal of the pipette tips from the attachment without excessive force.
  • the seating area extends to the upper edge of the collar, with which the pipette tip can be clamped onto the hub until it rests against the shoulder. According to another embodiment, the seating area extends to the inner step, with which the pipette tip can be clamped onto the hub until it rests against the shoulder.
  • the pipette tip family comprises a pipette tip designed to be pushed onto the neck until the upper edge of the collar rests against a shoulder, and at least one pipette tip of a different type designed to be pushed onto the neck until the inner step rests against a shoulder.
  • the pipette tip family comprises at least two Pipette tips of different types which are designed to be pushed onto the base of a pipette until an inner step rests on a shoulder.
  • each pipette tip has two concentric circumferential steps along its upper edge, with one step protruding further from the lower syringe opening than the other.
  • the pipette tip family comprises pipette tips with at least two of the following nominal volumes: 10 ⁇ l, 25 ⁇ l, 100 ⁇ l, 250 ⁇ l or 1,000 ⁇ l.
  • the pipette tip family includes pipette tips with nominal volumes of 10 ⁇ l, 100 ⁇ l, and optionally 1,000 ⁇ l, or 25 ⁇ l and 250 ⁇ l.
  • a particular advantage of these designs is that the mechanical display device can change the volume display for pipette tips with different nominal volumes by adjusting the marking to different decimal places.
  • the invention relates to a pipette system comprising a pipette according to one of claims 1 to 16 and at least one pipette tip from the pipette tip family according to one of claims 17 to 23.
  • the pipette system has the advantages of the pipette according to one of claims 1 to 16 and the pipette tips of the pipette tip family according to one of claims 17 to 23.
  • top and bottom refer to an arrangement of the positive displacement pipette in which the plug is vertically aligned and located at the downward-facing end of the pipette body.
  • these terms refer to a vertical alignment of the pipette tip's central axis, with the tip opening at the bottom and the plug-in opening at the top.
  • a pipette 1 designed as a positive displacement pipette has a substantially stem-shaped pipette housing 2, the upper part of which is shaped as a handle 3 and the lower part as a shaft 4.
  • a first shoulder 6 runs around the outer circumference of the pin, and a second shoulder 7 extends above it.
  • a through-channel 8 extends vertically from the lower end of the pin 5 into the pipette housing 2.
  • a first adjustment element 10 in the form of a sleeve-shaped adjustment knob of an adjustment device 11 for adjusting the measuring stroke is arranged below the operating element 9 at the upper end of the pipette housing 2. The operating element 9 can be pressed almost completely vertically into the first adjustment element 10.
  • a lifting rod 12 engages from above into the through-channel 8 of the pin 5. This rod extends upwards in the pipette housing 2 and is connected to the underside of the operating element 9.
  • the lifting rod 12 comprises an upper lifting rod part 13 and a lower lifting rod part 14.
  • the upper lifting rod part 13 is inserted from above into a vertical first bore 15 which extends longitudinally through the entire lower lifting rod part 14, so that the upper lifting rod part 13 is displaceable in the lower lifting rod part 14.
  • a lower section of a tubular cage figs 16 is fixed in an upper section of a connecting element 17 in the form of a reducing sleeve (e.g. by screwing).
  • the lower end of the cage figs 16 rests against a first projection 18 of the connecting element 17, which is located on the inner circumference.
  • the lower lifting rod part 14 is fixed in the lower section of the connecting element 17 (e.g., by pressing in).
  • the upper section of the connecting element 17 has larger inner and outer diameters than the lower section, and the connecting element 17 has a third shoulder 19 on the outer circumference between them.
  • the Kä Figure 16 is limited at the top by a radially inwardly projecting, circumferential second projection 20.
  • the upper lifting rod part 13 is connected by the cage Figure 16 and the connecting element 17.
  • the upper lifting rod part 13 has a circumferential bead 21, which is inserted into the cage Figure 16 is arranged.
  • a preloaded first coil spring 22 sits on the upper lifting rod part 13 between the bead 21 and the first projection 18. This presses the bead 21 against the second projection 20.
  • the top of the Kä figs 16 is a first stop element 23. Arranged above this is a spindle nut 24 fixed in the pipette housing 2. A threaded spindle 25 engages the spindle nut 24 and has a second bore 26 through which the upper lifting rod part 13 is passed. The lower end face of the threaded spindle 25 forms a second stop element 27.
  • the spindle nut 24 and the threaded spindle 25 are components of a first gear for adjusting the vertical position of the second stop element 27.
  • the first adjustment element 10 is fixedly connected to an adjustment sleeve 28. This is coupled to the threaded spindle 25 via a drive device 29 with axially extending grooves 30 on the inside of the adjustment sleeve 28 and radially outwardly projecting wings 31 engaging therein.
  • the drive device 29 is coupled to the threaded spindle 25 in a rotationally fixed manner and is vertically displaceable relative to one another. Consequently, by rotating the first adjustment element 10, the axial position of the second stop element 27 in the pipette housing 2 can be adjusted without axially displacing the first adjustment element 10.
  • the upper lifting rod part 13 is passed through the spindle nut 24 and is firmly connected to the operating element 9 so that it can be displaced vertically downwards by pressing the operating element 9.
  • the lower end face of the connecting element 17 is a third stop element 37.
  • a cup-shaped fourth stop element 38 is held in the pipette housing 2 below the connecting element 17.
  • the fourth stop element 38 has a central through-hole 39 in its base 40, through which the lower lifting rod part 14 extends.
  • the fourth stop element 38 is held in place by a compensating spring 41 acting on its upper edge and supported in the pipette housing 2, via a collar 42 extending around its outer circumference, on a fourth shoulder 43 in the pipette housing 2.
  • the third stop element 37 and the fourth stop element 38 together form a second stop device 44, which limits the downward displacement of the lower lifting rod part 14.
  • the lifting rod 12 and the operating button 9 are part of a drive device 46 for a piston.
  • a scanning device 47 is mounted on the pipette housing 2.
  • This device has a scanning element 48 formed by the lower end of a slide 49.
  • the slide 49 has a slide sleeve 50 guided on the shaft 4 at the bottom and a diaphragm 51 at the top.
  • the slide sleeve 50 and the diaphragm 51 are connected to one another via a rod 52.
  • the rod 52 is fixed at the bottom in a projection 53 protruding laterally from the slide sleeve 50.
  • a web-like graduation mark 54 of the diaphragm 51 forms a marking 55 that can be set behind the lowest pre-decimal place and/or in front of the highest decimal place of the counter 34.
  • the aperture 51 is arranged on the inside of the pipette housing 2 outside in front of the counter 34, so that the graduation mark 54 is visible from the outside through the first window 36.1 of the pipette housing 2, which is covered by a transparent pane.
  • a flag 56 At the upper end of the aperture 51 is a flag 56 on which several different nominal volume indications 57, 58 are arranged one above the other.
  • One nominal volume indication 57, 58 is visible from the outside through a second window 36.2 of the pipette housing 2, which is covered by a transparent pane.
  • the rod 52 of the slide 49 carries a radially projecting disc 59 which forms a fifth stop element 60.
  • the projection 53 is arranged through a shaft opening 61 at the lower end of a shaft 62 arranged laterally on the pipette housing 2.
  • the rod 52 is guided through a hole in a horizontal partition 63 of the pipette housing 2.
  • the top of the partition 63 (in Fig. 4 (additionally entered) is a sixth stop element 64, which limits the downward displacement of the slide 49 by engaging the fifth stop element 60.
  • the fifth stop element 60 and the sixth stop element 64 together form a third stop device 65.
  • a second spring device 66 in the form of a second helical spring 67 is supported at a third engagement point at the top on a holder 68 of the pipette housing 2 for the counter 34 and at a fourth engagement point at the bottom on a horizontal frame part 69 of the panel 51.
  • the second helical spring 67 is pretensioned to hold the fifth stop element 60 in contact with the sixth stop element 64.
  • a pipette tip 70 has according to Fig. 2 and 3 a cylindrical piston running area 71. At the lower end of the cylindrical piston running area 71, there is a conical starting section 72. The tip opening 73 is centrally located at the lower end of the starting section 72.
  • the piston running area 71 is connected at the top to a conical transition area 74, which is followed by a cylindrical connecting area 75.
  • the cylindrical connecting area 75 is connected to a collar 77 via an extension 76.
  • the collar 77 has a slightly conical shape that widens upwards. On its inner circumference, it has a circumferential bead 78, which belongs to or forms a seating area 78.1.
  • a piston 79 is arranged in the pipette tip 70.
  • This has a conical piston section 80 which can be inserted into the cylindrical piston running area 71.
  • the piston section 80 is connected to the Piston running area 71 is sealed.
  • the piston section 80 can be inserted into the conical initial section 72 of the pipette tip 70.
  • the piston section 80 is connected to a conical connecting section 82, which can be inserted into the transition area 74.
  • a short piston rod 83 projects upwards from the transition area 74.
  • Pipette tips 70 with different nominal volumes have cylindrical piston running areas 71 and corresponding conical piston sections 80 with different cross-sections. They also differ in that the collar 77 has a different height relative to the seat area 78.1.
  • the piston rod 83 is connected to the lifting rod 12 by pressing the operating element 9 until the second stop device 44 hits. In this case, the piston rod 83 is clamped into the first bore 15 of the lifting rod 12. This is shown in Fig. 2 shown.
  • the lower end of the pipette tip 70 is inserted into another vessel and the liquid is passed through Pressing the operating element 9 until the second stop device 44 hits the stop.
  • the piston 79 is moved to its lowest position in the pipette tip 70 and the liquid volume is displaced through the tip opening 73 into the vessel.
  • the lifting rod 12 is coupled to a piston, which can be displaced in a cylinder arranged in the pipette housing 2 by actuating the operating element 9.
  • the cylinder is connected via a channel to a hole in the lower end of the pin 5.
  • the sensing device 47 can be designed such that it has a sensing element 48 at the lower end of a slide 49, which has a slide sleeve 50 guided on the shaft 4.
  • the slide 49 is coupled at the top to a marking 55 for setting laterally next to a counter roller 35 of a counter 34 and, if necessary, to a flag 56 for displaying various nominal volume values 57, 58 in a window.
  • the slider 49 can also be used to eject the pipette tip 70.
  • the slider 49 is Fig. 9 coupled to an ejection button 85 that protrudes outward from the pipette housing 2.
  • the ejection button 85 can be rigidly connected to the slide 49. However, this has the consequence that the position of the ejection button 85 depends on the height of the collar 77 of the respective attached pipette tip 70.
  • a transmission device 89 is provided between the ejection button 85 and the slide 49, which, when the slide 49 is displaced upward, does not displace the ejection button 85 upwards and, when the ejection button 85 is displaced downwards, also displaces the slide 49 downwards.
  • the transmission device 89 comprises a fourth spring device 90 between the release button 85 and the rod 52 of the slider 49, which is designed as a third coil spring 91.
  • the fourth spring device 90 is pre-tensioned, so that when the ejector button 85 is not pressed in, the fourth stop device 86 is effective.
  • the slide 49 is displaced upwards and the fourth spring device 90 is compressed.
  • the ejector button 85 remains in its upper stop position.
  • the fourth spring device 90 is initially further compressed. The ejector button 85 then presses on the rod 52 of the slide 49, causing the latter to strip the pipette tip 70 from the pin 5.
  • the transmission device 89 comprises a locking device 92 arranged between the release button 85 and the slide 49.
  • This locking device is a gear 93 rotatably mounted on the release button 85, which has a freewheel 94.
  • the rod 52 has a toothed strip 95 on one side, which engages with the gear 93.
  • the fourth stop device 86 is activated by a fifth spring device 96 acting on it.
  • the slide 49 is displaced upwards, whereby the gear wheel 93 rotates freely. Only when the ejector button 85 is actuated is the Free rotation of the gear 93 is stopped and the rod 52 is moved downward. As a result, the slide 49 pushes the pipette tip 70 off the pin 5.
  • Fig. 14 Displacement of the ejector button 85 when attaching the pipette tip 70 is prevented by the fact that an ejector slide 97 is provided in addition to the slide 49.
  • the slide 49 has an inner slide sleeve 98 guided on the shaft at its lower end, and the ejector slide 97 has an outer slide sleeve 99 guided on the inner slide sleeve 98 at its lower end.
  • the pipette tip 70 has two concentric circumferential steps 100, 101 at the upper edge, with the inner step 100 projecting further upwards in the vertical direction than the outer step 101.
  • the inner step 100 actuates the slide 49, thereby setting the graduation mark 54 on the mechanical counter 34.
  • the ejector slide 97 is not actuated by the outer step 101.
  • the ejector button 85 is pressed, the lower end of the ejector slide 97 strikes the outer step 101 and pushes the pipette tip 70 off the pin 5.
  • a pipette 1 designed as a positive displacement pipette comprises a pipette housing 2 with an operating element 9 designed as a vertically pivotable operating lever 102 on a vertical side surface and a first adjustment element 10 designed as a rotary knob 103 for setting a volume at the upper end.
  • a positive displacement pipette tip 70 can be clamped onto the pin 5 at the bottom.
  • a set volume can be drawn into the pipette tip 70.
  • a spring is arranged in the pipette housing 2, which is released by pressing the operating lever 102 downwards. is tensioned and pivots the operating lever 102 upward after the tension is released.
  • the length of the movement of the operating lever 102 and/or the number of times it is actuated depends on the set volume.
  • the drawn-up liquid can be dispensed from the pipette tip 70. The actuation or the number of actuations, in turn, depends on the set volume.
  • the drive device of the pipette 1 has a switching device which reverses the direction of displacement of the piston 79 by actuating the operating lever 102 after the set volume has been sucked into the pipette tip 70. Consequently, further actuation of the operating lever 102 causes the ejection of the aspirated liquid from the pipette tip 70.

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Claims (22)

  1. Pipette destinée à être utilisée avec une pointe de pipette d'une famille de pointes de pipette comportant des pointes de pipette de différents types, comportant
    • un boîtier de pipette (2) en forme de tige,
    • un tenon (5) à l'extrémité inférieure du boîtier de pipette (2), sur lequel peut être clipsée une pointe de pipette (70) dotée d'un col (77) à son extrémité supérieure, lequel présente une ouverture d'enfichage (84),
    • un dispositif d'entraînement (46) comportant un élément d'entraînement (12) pour le déplacement d'un élément de refoulement (79) pour l'aspiration d'échantillons liquides dans la pointe de pipette (70) et l'expulsion des échantillons hors de la pointe de pipette (70), ainsi qu'un élément de commande (9) faisant saillie à partir du boîtier de pipette (2), déplaçable par rapport au boîtier de pipette,
    • un dispositif d'exploration (47) doté d'un élément d'exploration (48) disposé à côté du tenon (5), déplaçable dans la direction longitudinale du tenon (5) par rapport au boîtier de pipette, destiné à être déplacé à travers le col (77) d'une pointe de pipette (70) lors du clipsage de la pointe de pipette (70) sur le tenon (5),
    • un dispositif d'affichage mécanique (34) accouplé au dispositif d'exploration (47), le dispositif d'exploration (47) étant conçu de manière à régler un affichage du dispositif d'affichage (34), lequel dépend de la position de l'élément d'exploration (48) par rapport au boîtier de pipette (2),
    pour le réglage d'un affichage dépendant du type de la pointe de pipette (70) par clipsage d'une pointe de pipette (70) avec une hauteur du col (77) caractéristique du type de la pointe de pipette (70) sur le tenon (5),
    caractérisée par
    • un premier dispositif de butée (32) doté d'un premier élément de butée (23) sur le dispositif d'entraînement (46) et d'un deuxième élément de butée (27) sur le boîtier de pipette (2), lesquels peuvent être mis en butée l'un contre l'autre par déplacement de l'élément d'entraînement (12) dans une première direction,
    • un deuxième dispositif de butée (44) doté d'un troisième élément de butée (37) sur le dispositif d'entraînement et d'un quatrième élément de butée sur le boîtier de pipette (2), lesquels peuvent être mis en butée l'un contre l'autre par déplacement de l'élément d'entraînement (12) dans une deuxième direction opposée à la première direction, afin de définir une course de mesure du dispositif d'entraînement (46) conjointement avec le premier dispositif de butée (32),
    • un dispositif de réglage (11) doté d'un premier élément de réglage (10) monté sur le boîtier de pipette (2) et d'un deuxième élément de réglage sur le premier ou le deuxième dispositif de butée (32, 44), pour le réglage de la course de mesure par réglage du premier élément de réglage (10),
    • un compteur mécanique (34) accouplé au dispositif de réglage (11) pour l'affichage du réglage du dispositif de réglage (11),
    • dans laquelle le dispositif d'exploration (47) est accouplé à un dispositif mécanique de réglage de zone sur le compteur (34), lequel est conçu de manière à régler une plage de comptage du compteur (34), laquelle dépend de la position de l'élément d'exploration (48) par rapport au boîtier de pipette.
  2. Pipette selon la revendication 1, dans laquelle l'affichage dépendant du type de la pointe de pipette (70) comporte ou est un affichage de volume.
  3. Pipette selon la revendication 1 ou 2, dans laquelle le dispositif d'exploration (47) présente un poussoir (49) guidé de façon déplaçable dans la direction verticale sur le boîtier de pipette (2), lequel présente l'élément d'exploration (48) à l'extrémité de poussoir inférieure et dont l'extrémité de poussoir supérieure est accouplée au dispositif d'affichage (34).
  4. Pipette selon la revendication 3, dans laquelle, sur un côté extérieur, le poussoir (49) porte différentes indications de volume nominal (57, 58) ou différentes autres indications caractérisant le type de la pointe de pipette (70) les unes au-dessus des autres dans la direction verticale et le boîtier de pipette (2) présente une fenêtre (36) dans la zone de déplacement des indications de volume nominal ou des autres indications du poussoir (49), à travers laquelle seule une indication de volume nominal (57, 58) complète ou une autre indication complète est respectivement visible depuis l'extérieur sur le poussoir (49).
  5. Pipette selon l'une des revendications 3 et 4, dans laquelle le dispositif de réglage de zone comporte un marquage (55) disposé à l'extérieur devant le compteur (34) à l'extrémité de poussoir supérieure, moyennant quoi, lors du clipsage de pointes de pipette (70) dotées de cols (77) de différentes hauteurs sur le tenon (5), le poussoir (49) peut être déplacé à différentes distances vers le haut, afin de positionner le marquage (55) pour l'affichage de différentes zones de comptage latéralement à côté de divers rouleaux de compteur (35) du compteur (34) ou entre ceux-ci.
  6. Pipette selon l'une des revendications 3 à 5, dans laquelle l'élément d'exploration (48) est réalisé à l'extrémité de poussoir inférieure d'une douille de poussoir (50), laquelle peut être décalée dans la direction verticale le long d'un arbre (4) du boîtier de pipette (2) présentant le tenon (5) à l'extrémité inférieure.
  7. Pipette selon la revendication 5 ou 6, dans laquelle le marquage est un trait partiel (54) semblable à une nervure, avec un cache (51) relié à l'extrémité de poussoir supérieure.
  8. Pipette selon l'une des revendications 1 à 7, comportant un premier dispositif à ressort (45) doté d'un premier point d'attaque sur le boîtier de pipette (2) et d'un deuxième point d'attaque sur le dispositif d'entraînement (46) pour déplacer l'élément d'entraînement (12) dans la première direction jusqu'à l'application du premier élément de butée (23) sur le deuxième élément de butée (27) lorsque l'élément de commande (9) est soulagé.
  9. Pipette selon l'une des revendications 3 à 8, laquelle présente un troisième dispositif de butée (65) doté d'un cinquième élément de butée (60) sur le poussoir (49) et d'un sixième élément de butée (64) sur le boîtier de pipette (2) ainsi qu'un deuxième dispositif à ressort (66) doté d'un troisième point d'attaque sur le boîtier de pipette (2) et d'un quatrième point d'attaque sur le poussoir (49) pour solliciter le poussoir (49) vers le bas et appliquer le cinquième élément de butée (60) contre le sixième élément de butée (64) lorsqu'aucune pointe de pipette (70) n'est clipsée sur le tenon (5).
  10. Pipette selon l'une des revendications 3 à 9, dans laquelle le poussoir (49) est relié sur le haut à un bouton d'éjection (85) faisant saillie à partir de l'extrémité supérieure du boîtier de pipette (2), par l'actionnement duquel le poussoir (49) peut être déplacé vers le bas pour enlever une pointe de pipette (70) du tenon (5) avec l'extrémité de poussoir inférieure.
  11. Pipette selon les revendications 3 et 10, laquelle présente un quatrième dispositif de butée (86) doté d'un septième élément de butée (87) sur le bouton d'éjection (85) et d'un huitième élément de butée (88) sur le boîtier de pipette (2), lesquels sont conçus pour limiter le déplacement du bouton d'éjection (85) vers le haut, et laquelle présente un dispositif de transmission (89) entre le bouton d'éjection (85) et le poussoir (49), lequel est conçu pour ne pas déplacer le bouton d'éjection (85) vers le haut lors d'un déplacement du poussoir (49) vers le haut et pour déplacer le poussoir (49) conjointement vers le bas lors d'un déplacement du bouton d'éjection (85) vers le bas.
  12. Pipette selon les revendications 3 et 11, (i.) dans laquelle le dispositif de transmission (89) est un quatrième dispositif à ressort (90), lequel présente un cinquième point d'attaque sur le bouton d'éjection (85) et un sixième point d'attaque sur le poussoir (49), moyennant quoi, lors d'un déplacement du poussoir (49) vers le haut, le quatrième dispositif à ressort (90) est comprimé et lors d'un déplacement du bouton d'éjection (85) vers le bas, le quatrième dispositif à ressort (90) est d'abord comprimé et ensuite le bouton d'éjection (85) entraîne le poussoir (49), ou (ii.) dans laquelle le dispositif de transmission (89) est un moyen de verrouillage (92) disposé entre le bouton d'éjection (85) et le poussoir (49), lequel permet un déplacement du poussoir (49) vers le haut par rapport au bouton d'éjection (85) et empêche un déplacement du bouton d'éjection (85) vers le bas par rapport au poussoir (49).
  13. Pipette selon l'une des revendications 3 à 9, laquelle présente, en plus du poussoir (49), un poussoir d'éjection (97) guidé dans la direction verticale sur le boîtier de pipette (2), lequel est relié au bouton d'éjection (85) à l'extrémité supérieure et dont l'extrémité inférieure est disposée à côté du tenon, en décalage radialement par rapport à l'extrémité inférieure du poussoir (49), dans laquelle (i.) les extrémités inférieures du poussoir (49) et du poussoir d'éjection (97) sont disposées à la même hauteur, de sorte que lors du clipsage d'une pointe de pipette (70) dotée de deux gradins périphériques (100, 101) à l'extrémité supérieure sur le tenon (5), le poussoir (49) peut être déplacé par le gradin intérieur (100) et le poussoir d'éjection (97) ne peut pas être déplacé par le gradin extérieur (101) et l'extrémité inférieure du poussoir d'éjection (97) peut être poussée contre le gradin extérieur (101) de la pointe de pipette (70) par actionnement du bouton d'éjection (85), pour enlever celle-ci du tenon (5), ou (ii.) l'extrémité inférieure du poussoir (49) est disposée plus bas que l'extrémité inférieure du poussoir d'éjection (97), de sorte que lors du clipsage d'une pointe de pipette (70) dotée d'un bord supérieur plat sur le tenon (5), le poussoir (49) peut être déplacé vers le haut et le poussoir d'éjection (97) ne peut pas être déplacé vers le haut et le poussoir d'éjection (97) peut être poussé contre l'extrémité supérieure de la pointe de pipette (70) par actionnement du bouton d'éjection (85) et la pointe de pipette (70) peut être enlevée de l'embout (5).
  14. Pipette selon l'une des revendications 1 à 13, dont l'embout (5) présente un épaulement périphérique (7) sur le pourtour extérieur, à une distance de l'extrémité inférieure, de sorte qu'une pointe de pipette (70) dotée d'un gradin intérieur périphérique (76) faisant saillie radialement vers l'intérieur sur le pourtour intérieur du col (77) peut être glissée sur l'épaulement (7) jusqu'à l'application du gradin intérieur (76).
  15. Famille de pointes de pipette destinée à être utilisée avec une pipette comportant au moins deux pointes de pipette (70) de différents types, dans laquelle chaque pointe de pipette (70) présente une ouverture de pointe (73) à l'extrémité inférieure, un col (77) présentant une ouverture d'enfichage (84) avec une hauteur caractéristique du type de la pointe de pipette (70) à l'extrémité supérieure et une région de siège sur le pourtour intérieur du col (77) pour le clipsage sur un tenon (5) avec un contour déterminé d'une pipette (1), caractérisée en ce que des pointes de pipette (70) de différents types peuvent être clipsées avec leur région de siège (78.1) sur la même zone de clipsage (5.1) du tenon (5), de manière à se dresser à différentes hauteurs sur le tenon (5) avec leur col (77).
  16. Famille de pointes de pipette selon la revendication 15, comportant au moins deux pointes de pipette (70), lesquelles se distinguent de par l'une ou plusieurs des caractéristiques suivantes : volume nominal, forme, dimensions, matériau, sans ou avec revêtement, sans ou avec traitement de surface, petits tubes sans ou avec filtre intégré, électriquement isolantes ou électriquement conductrices, degré de pureté.
  17. Famille de pointes de pipette selon l'une des revendications 15 ou 16, dans laquelle la différence de hauteur de la hauteur avec laquelle le col (77) de pointes de pipette (70) avec divers volumes nominaux se dresse sur le même tenon (5) est égale au simple ou à un multiple entier de la hauteur d'un rouleau de compteur (35) d'un compteur mécanique (34).
  18. Famille de pointes de pipette selon l'une des revendications 15 à 17, dans laquelle la différence de hauteur s'élève au simple ou à un multiple entier de 1 à 7 mm, de préférence de 3 à 5 mm, de préférence de 4,5 mm.
  19. Famille de pointes de pipette selon l'une des revendications 15 à 18, dans laquelle, au-dessus de la région de siège (78.1), le col (77) présente un bord supérieur ou un gradin intérieur périphérique (76.1) faisant saillie radialement vers l'intérieur sur le pourtour intérieur, et le col (77) présente une section élargie au-dessus du gradin intérieur (76.1), de sorte que la pointe de pipette (70) peut être glissée sur l'embout (5) jusqu'à l'application du bord supérieur ou du gradin intérieur (76.1) sur un épaulement (7) de celui-ci.
  20. Famille de pointes de pipette selon l'une des revendications 15 à 19, dans laquelle chaque pointe de pipette (70) présente deux gradins périphériques concentriques (100, 101) sur le bord supérieur, dans laquelle un gradin (100) fait saillie plus loin vers le haut par rapport à l'ouverture de pointe inférieure (73) que l'autre gradin (101).
  21. Famille de pointes de pipette selon l'une des revendications 15 à 20, laquelle présente une région cylindrique de course de piston (71) entre l'ouverture de pointe (73) et l'ouverture d'enfichage (84) ainsi qu'un piston (79) doté d'une tige de piston (83) à l'extrémité supérieure, pour le clipsage dans un trou d'une tige de levage (12) d'une pipette (1) conçue comme une pipette à déplacement positif.
  22. Système de pipette comportant une pipette selon l'une des revendications 1 à 14 et au moins une pointe de pipette (70) issue de la famille de pointes de pipette selon l'une des revendications 15 à 21.
EP19718400.5A 2018-04-23 2019-04-23 Pipette à utiliser avec une pointe de pipette et une famille de pipettes comprenant plusieurs pipettes avec des volumes nominaux différents Active EP3784399B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP18168763.3A EP3560596A1 (fr) 2018-04-23 2018-04-23 Pipette à utiliser avec une pointe de pipette et une famille de pipettes comprenant plusieurs pipettes avec des volumes nominaux différents
PCT/EP2019/060324 WO2019206879A1 (fr) 2018-04-23 2019-04-23 Pipette pour utilisation avec une pointe de pipette et famille de pointes de pipette comprenant plusieurs pointes de pipette de type différent pour utilisation avec une pipette

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EP3784399A1 EP3784399A1 (fr) 2021-03-03
EP3784399B1 true EP3784399B1 (fr) 2025-06-25

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EP18168763.3A Withdrawn EP3560596A1 (fr) 2018-04-23 2018-04-23 Pipette à utiliser avec une pointe de pipette et une famille de pipettes comprenant plusieurs pipettes avec des volumes nominaux différents
EP19718400.5A Active EP3784399B1 (fr) 2018-04-23 2019-04-23 Pipette à utiliser avec une pointe de pipette et une famille de pipettes comprenant plusieurs pipettes avec des volumes nominaux différents

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EP (2) EP3560596A1 (fr)
JP (1) JP7407730B2 (fr)
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WO (1) WO2019206879A1 (fr)

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EP3831487B1 (fr) * 2019-12-06 2024-02-07 Eppendorf SE Pipette à utiliser avec une pointe de pipette et pointe de pipette à utiliser avec une pipette
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US20250186991A1 (en) 2025-06-12
CN112020394A (zh) 2020-12-01
US12325018B2 (en) 2025-06-10
JP2021520994A (ja) 2021-08-26
EP3560596A1 (fr) 2019-10-30
US20230110524A1 (en) 2023-04-13
EP3784399A1 (fr) 2021-03-03
CN112020394B (zh) 2022-07-08
JP7407730B2 (ja) 2024-01-04
WO2019206879A1 (fr) 2019-10-31

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