[go: up one dir, main page]

EP0046461B1 - Dispositifs de dosage pour liquides ou gaz - Google Patents

Dispositifs de dosage pour liquides ou gaz Download PDF

Info

Publication number
EP0046461B1
EP0046461B1 EP80105031A EP80105031A EP0046461B1 EP 0046461 B1 EP0046461 B1 EP 0046461B1 EP 80105031 A EP80105031 A EP 80105031A EP 80105031 A EP80105031 A EP 80105031A EP 0046461 B1 EP0046461 B1 EP 0046461B1
Authority
EP
European Patent Office
Prior art keywords
spindle
piston
pipette
dosing
housing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
EP80105031A
Other languages
German (de)
English (en)
Other versions
EP0046461A1 (fr
Inventor
Winfried Dr. med. Stöcker
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
STOECKER, WINFRIED, DR. MED.
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to AT80105031T priority Critical patent/ATE22402T1/de
Priority to EP80105031A priority patent/EP0046461B1/fr
Priority to DE8080105031T priority patent/DE3071776D1/de
Publication of EP0046461A1 publication Critical patent/EP0046461A1/fr
Application granted granted Critical
Publication of EP0046461B1 publication Critical patent/EP0046461B1/fr
Expired legal-status Critical Current

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0234—Repeating pipettes, i.e. for dispensing multiple doses from a single charge

Definitions

  • a separate piston-stroke pipette was initially used for each different volume. Pipettes that can dose several volumes were later used. Your piston stroke can be changed by adjusting the stops, either in steps (e.g. model 1100 V from Kontron, Eching, Germany) or continuously (e.g. model 3100 from Kontron).
  • the piston stroke can be infinitely precisely adjusted by means of a micrometer (eg US Pat. No. 3,334,788 or Finnpipette 0 from Müller ratiolab, Dreieich, Germany).
  • the capillary-piston pipettes do not dose without carryover.
  • Your pistons and cylinders are wetted by the metered liquid (e.g. Capilettor® from Labora Mannheim, Germany or Transferpettor m from Brand, Wertheim, Germany). But they do not share the disadvantages that the air cushion brings with it.
  • the metered liquid e.g. Capilettor® from Labora Mannheim, Germany or Transferpettor m from Brand, Wertheim, Germany.
  • the piston stroke is also fixed in these pipettes, or it can be adjusted in steps or continuously.
  • the "Hamilton dispenser” was copied several times without significant improvements (e.g. DE-A 27 36 551 and DE-A 25 25 670). It was further developed by the company Eppendorf-Gerätebau, Hamburg, Germany: In the Multipette 478001, the driving rack and the syringe lie in the same axis. The volume to be dispensed in one step can be changed in several steps without changing the syringe. To do this, you push a metal tongue in front of the rack at different distances, thereby determining the distance by which the rack is moved by the pawl of a ratchet mechanism.
  • the dosing volume can be varied if syringes with different internal cross-sections are used, which are made of plastic on the Multipette 4780 (!), Can be easily exchanged and discarded after use.
  • the Multipette 4780 v can only dose precisely in one direction and the syringe used in the plunger is not secured against unintentional shifting during the dosing pause.
  • the dosing volume can be easily determined by two stops that limit the piston stroke up and down. If a liquid is to be taken up or dispensed several times in succession (collector or dispensing pipette), the piston in the cylinder must always be able to move the same distance in the same direction. You could use a cylinder with a scale for this. Before each step one would have to calculate the next scale value and then move the piston until then (e.g. dosing micrometer from Desaga, Heidelberg, Germany). However, this is much faster and more precise with the help of a grid mechanism.
  • the rack of the "Hamilton dispenser” contains, for example, a linear grid, in which the propulsion mechanism engages directly in order to move the rack ("active grid”). With each dosing step, a spring-loaded pawl moves it by a distance equal to the distance between two teeth. However, the rack can only be moved in one direction in precise steps corresponding to the grid. If it should also be shifted by exact distances in the opposite direction, then it is advisable to use a "passive grid” that is not part of the drive mechanism, but only marks the feed, cf. e.g. B. Fig. 1. "Passive detent mechanisms” are used, for example, in tap changers of electrical devices (kitchen range) or in lens change revolvers (microscope).
  • the typewriter of a typewriter can also be rotated by a ratchet mechanism, the catch of which engages in a circular (“active") grid and moves it by one or more teeth, comparable to the linear displacement of the rack in the "Hamilton dispenser” (only one tooth) or in the Eppendorf Multipette (one tooth or several teeth).
  • a rotating ratchet mechanism can also be used in connection with a spindle system to construct a metering device, as is shown in this invention (see Fig. 4).
  • the stops which limit the stroke can be set precisely in piston-stroke pipettes (US Pat. No. 3,334,788 and DE-A 26 32 970).
  • Spindle systems are also used to directly move the plunger in a syringe barrel, e.g. in the dosing micrometer from Desaga, Heidelberg, Germany, or in many motor-driven dosing devices.
  • the piston can still be moved very long distances if the spindle is rotated several turns. This enables you to dose very small and very large volumes in succession with the same syringe.
  • spindles can be used to slide the piston in the cylinder almost without jerking (DE-B 27 31 102):
  • a piston seal can be easily elastically deformed and the coefficient of static friction between the cylinder and the piston seal is high, then the piston moves jerkily in the cylinder instead of sliding smoothly. Even with a good syringe, the piston jerks a little when it has overcome the static friction resistance. The resulting error can be reduced using a screw mechanism.
  • a thread between the piston and the cylinder links the axial feed of the piston with a rotary movement, and the axially directed jerk at the beginning of each metering step is reduced to a fraction in accordance with the radius and the pitch of the thread.
  • Spindle systems are particularly suitable for the construction of motor-driven dosing devices. However, toothed racks with worm drive and other systems are also used. With good devices, the plunger in the syringe housing can be moved exactly in both directions. The smallest defined stroke displacements can be achieved with a transmission gear or with a spindle whose thread rises very flat. The spindle can be parallel to the syringe plunger or better in the same axis.
  • DE-2 439 269 describes a drug injection device in which the syringe plunger is displaced in connection with the rotary movement of an engine in an injection syringe.
  • the volume delivery is controlled by an electric speed counter.
  • CH-A-607 620 describes a metering device in which a piston in a cylinder is displaced by a motor-driven spindle mechanism.
  • the adjustment movement of the spindle mechanism required for volume adjustment is defined here by stops on two scale rings.
  • stepper motors have been preferred for driving metering devices. They are controlled by positioning electronics and a high dosing accuracy is achieved with them.
  • motor-driven metering devices Microlab M m and Microlab P m from Hamilton, Bonaduz, Switzerland; Dosing unit of the "Automatic Clinical Analyzer” from Du Pont, Wilmington, Delaware, USA; Reagent dispenser 5211 from Eppendorf-Gedorfebau, Hamburg, Germany; for several syringes at the same time: RIA sample processor from LKB-Wallac, Turku, Finland; Autodrop-Titertek0 from Flow Laboratories, Bonn.
  • Most motorized dosing devices are very versatile because they can dose precisely in the pick-up and discharge directions and because they cover a large volume range. But they are also technically complex and therefore expensive, they take up a lot of work space and can usually not be transported as easily from one laboratory place to another as a handheld device.
  • Another Multititer® pipette marketed by Flow Laboratories, Bonn, Germany, combines several of the piston stroke pipettes described above. These pipettes are also available with different handles for different volumes to be dosed (Finnipette®, DE-A 2 700 096) or with a continuously adjustable stroke (Titerteklb multichannel pipetting handle from Flow Laboratories, Bonn, Germany).
  • the company Hamilton (Bonaduz, Switzerland) sells a "Terasaki dispenser” with which one can operate six syringes next to each other according to the principle of the "Hamilton dispenser” described above.
  • This device lacks precise guidance: If it is difficult to move the plunger in one of the syringes, the linkage will tilt and the pistons on the side of the stiff syringe will not be moved as far as it is intended, on the other side all the more . This leads to the delivery of different volumes in the different syringes.
  • the individual syringe cylinders can slip back a bit when the liquid to be dosed is drawn on.
  • the degree guide can be supported according to the invention by a parallel joint guide, as is known from presses for dishing purposes (Hallmann, UC, "Patent application made easy", 1977, Hans-Holzmann-Verlag Bad Wörirshofen, p. 48 ff).
  • peristaltic pumps It is also possible to pipette several samples simultaneously with peristaltic pumps, but these devices should only be suitable for semi-quantitative dosing (e.g. peristaltic pump from Jungkeit, Nörten-Hardenberg, Germany, or Autospotter 0 from Desaga, Heidelberg, Germany) .
  • the devices intended for filling liquids according to DT-C 24 59 365, DE-A 28 00 771 and DE-A 27 25 835 are also not suitable for precise dosing in the microliter range.
  • the device described in DE-B 24 15 021 The same liquid is dripped into several vessels simultaneously using a micropipetting device. The volume delivery is imprecise and depends on the surface tension of the liquid to be pipetted. If a channel is blocked, the volume intended for this channel is distributed to the other channels.
  • the invention has for its object to provide a dosing device for liquids, which has a high degree of dosing accuracy and is functionally as versatile as a precision dosing device driven by stepper motors with positioning electronics.
  • the metering device of DE-B 2 439 269 which corresponds to the preamble of claim 1, is to be improved so that it can be operated by hand, the electrical pulse counting device being triggered by a signal which can be felt by the operator Limitation of the dosing volume has to be replaced.
  • Precision syringes are mainly used for the metering devices of this invention, the pistons (pistons) of which are displaced by a certain distance in the cylinder (housing) in a metering step.
  • the total stroke of each piston is broken down into a certain number of sections.
  • the plunger is displaced by a spindle system in the syringe housing.
  • a certain piston feed is assigned to each angle of rotation of a spindle against a spindle nut.
  • the angle of rotation can be limited by two stops (see Fig. 4) or by two races of a circular "passive raster".
  • the circular "passive locking mechanism” fulfills the same function as a control circuit in a dosing device with a stepper motor. It divides the piston stroke into arbitrarily small defined distances and allows certain volumes to be taken up or released one or more times in succession. Different volumes can be dosed in succession with pipettes that contain several "passive locking mechanisms" without having to change syringes between the pipetting steps (Fig. 1). These pipettes are therefore also suitable as dilutors, apart from the fact that they can also be used as transfer, dispensing and collector pipettes.
  • the spindle system can bring the additional advantage that the piston can be moved almost without jerking in the axial direction because it can be rotated in the cylinder at the same time (Fig. 2).
  • Constructions are proposed that allow a continuous adjustment of the dosing volumes (Fig. 3 and Fig. 4).
  • the pipettes can be equipped with permanently installed piston lifting units or with precision syringes or with syringes that are completely or partially discarded when changing samples. You can also dose with disposable pipette tips to be attached.
  • the dosing devices of this invention have, as a central component, one or more elongated housings, in each of which a stamp slides tightly.
  • the stamp divides the housing into two rooms. One space is open to the outside for pressure equalization, the other is filled with the gas or liquid to be metered during metering, or it is with the liquid to be metered in a separate filling chamber through a cavity system that is otherwise closed to the outside, if necessary, connected via an air cushion.
  • Components of this cavity system can be cannulas, tubes or pipette tips.
  • the housing and plunger are firmly integrated in the pipette body, or interchangeable precision syringes of any diameter are used, with hollow cylinders made of glass or metal or plastic and with pistons, the seals of which are made of plastic, rubber or metal.
  • Disposable plastic syringes with rubber piston seals can be used, which are exchanged as a whole each time the pipette is changed. For carryover-free pipetting, it is also sufficient if only the housing and the piston seal are replaced.
  • the piston rod is then firmly installed in the pipetting device.
  • the pipette can be provided with a ejection mechanism that strips off the housing and the piston seals. To do this, a ring is pushed along the piston rod or the piston rod is fully retracted. A similar mechanism may also be provided for dropping pipette tips.
  • a dosing volume proportional to the inner cross section of the housing is assigned to a certain displacement distance. This distance is determined by the distance between two detents or two stops.
  • a "passive ring locking mechanism" can, for. B. consist of a surface profile grid and a resilient locking follower, or also of a magnetic grid and one of the magnetic attraction or repulsion subjected to the locking follower. The distances between the catches or the stops can be infinitely adjusted (Fig. 3, 4). As a result, any volume can be measured with a certain syringe or the same volume with syringes of any internal cross-section.
  • Every pipetting step should still be able to be carried out completely, even in the extreme positions of the plunger in the syringe. This can be achieved by means of suitable suspension at the end points, which offer resistance to the last step but still allow it to be carried out completely, or by acoustic signals such as those built into typewriters to announce the end of the line.
  • the smallest volume that can be dosed with a syringe is equal to the product of the inner cross-section of the cylinder and the smallest precisely measurable feed of the piston in the cylinder.
  • syringes with a very small internal cross-section can therefore be used, or you can use a thicker syringe and move the plunger only very short distances.
  • Each defined piston feed corresponds to a certain distance in the measuring range of the pipette. However, very small distances cannot be measured precisely enough with simple mechanical means.
  • the desired reduction of the feed can be obtained by the spindle system: If a spindle is rotated 360 ° against a nut, then it shifts in the longitudinal direction by the distance h indicated with the thread pitch.
  • the circumference of the spindle covers the path 2 nm.
  • This path is 2 rn / h times longer than the axial feed. If the thread increases by 1 mm per 360 °, then there is 100 times more measuring distance available on the periphery of a ring of 31.8 mm diameter that is firmly mounted on the spindle than in the longitudinal direction. As a result, a volume that is a hundredth smaller than that possible in the axial direction can be measured. However, it is also possible to dose a much larger volume with the same pipette. You only need to turn the spindle through a larger angle, possibly over 360 °.
  • the spindle construction covers a very wide volume range and enables a multitude of smallest volumes to be dosed with one syringe filling.
  • Spindles have the further advantage that the slope of their housing can be adapted to the internal cross section of the syringes. Therefore, any syringe can be used to design dosing devices that are to pipette volumes in metric or in another system.
  • the principle of the "passive ring grid” can be used to save space.
  • a linear grid, namely without translation, must be as long as the distance by which the piston can be moved overall, because each catch is used only once.
  • a grid ring is rotated by several rounds during a total stroke and each detent is used several times.
  • a rotationally fixed spindle 401 is axially displaced by a rotating spindle nut 402 mounted in the pipette housing.
  • the longitudinal shaft 403 is rotated by the transverse shaft 404 via the bevel gear 405 and one of the two bevel gears 406 and 407.
  • the cross shaft is driven by a handwheel 408 (possibly combined micrometer-macrometer knurled wheel) or by a push button 409 with ratchet mechanism 470.
  • the actuation of the push button 409 rotates the spindle nut 402 clockwise or counterclockwise via the cylinder 414 attached to it.
  • the rotationally fixed spindle 401 and the syringe plunger 412 rigidly connected to it are pushed up or down.
  • the piston feed is divided by a ring locking mechanism into defined distances, the length of which is proportional to the angle of rotation taken by two adjacent detents of a set locking ring 413.
  • a spindle 803 fixed in the pipette body by two bearings 801 and 802 can be turned in both directions by means of a handwheel 804.
  • the spindle nuts 805 and 806 approach or move away from each other.
  • the spindle nuts are articulated to the legs of the scissor mechanism shown on the right, but are secured against rotation around the spindle axis (e.g. cross-drilled cylinders).
  • a piston 809 and a holder for a syringe housing 810 to be attached are fastened to two joints 807 and 808, which are moved against one another when the scissors are opened and closed.
  • the main joint 811 slides in an elongated hole guide 812 attached to the pipette body.
  • a ratchet ring 813 is fixedly mounted on the spindle. This can also be combined with the 804 handwheel.
  • the detents break down the entire piston stroke into sections of equal length (the centers of the spindle nuts describe an arc around the main joint 811 when the spindle is turned.
  • a second arc around the main joint describes the centers of the two joints on the left side of the figure.
  • the right bend is made according to the pitch of the spindle thread and the distance between two Rasteptept from each other, divided into a certain number of equally long distances. Each of these distances is assigned a certain section of the curve on the left, which is equal to the displacement distance of the piston against the cylinder.
  • This device can contain several grid rings with different grid divisions. It is also possible to continuously adjust the volume dosed with each click. For this purpose, the two left legs are shortened or lengthened telescopically after temporarily loosening the retaining screws 874 and 875, z. B. with two built-in micrometer screws. The further these legs are pulled out, the greater the volume dosed with each click, and the more the capacity of the syringe housing is used.
  • Ratchet drive with adjustable angle of rotation for a spindle system (Fig. 4)
  • the toothed ring is secured against rotation by the catch 1008.
  • the drive ring can be actuated, for example, directly by a handwheel or by a bellcrank, comparable to the paper quick feed lever of a typewriter. With a suitable suspension, the drive ring is turned back to the starting position at the end of each pipetting step. In this position, the pawl 1006 is located under a cover 1009, so that it does not hinder the turning back of the spindle when the syringe is filled or when, if necessary, a second, counter-rotating ratchet mechanism is activated.
  • Multi-channel pipettes can significantly increase the effectiveness of laboratory work and also help to make it easier to compare test results.
  • syringe housings 1502 there are two syringe housings 1502 in a housing block 1501, which are prevented from moving by a counterplate 1503.
  • a shaft 1507 is mounted in the stamp block, which can be turned by a handwheel 1508 (if necessary with coarse and fine drive).
  • the ratchet rings 1509 and 1510 and the bevel gears 1511 and 1512 rotate with the shaft.
  • the two spindles 1515 and 1516 are driven via the bevel gears 1513 and 1514, which bear a thread running in opposite directions (if the bevel gear 1511 is turned over and to the left of the bevel gear 1513 is installed, two identical spindles can be used).
  • the spindle rotates in the spindle nuts 1517 and 1518 and thereby move the housing block 1501 and the stamp block 1505 parallel to or away from one another.
  • the pistons in both housings move the same distance.
  • a metering volume of 3 4A01 is noticeably identified by the locking mechanism when the handwheel is turned.
  • the device shown contains only two channels, but it is also possible to construct pipettes with a lot more syringes next to or behind one another. You can also install more locking rings or use other locking mechanisms and drives shown in the previous pictures.
  • the entire housing block with the __ _ _ _ syringe housings and piston seals can be replaced in a modular manner after loosening the 1519 screws.
  • the inner collars 1520 of the syringe housing strip off the piston seals 1521 from the piston rods.
  • the plug-in profiles of the piston rods are pressed into the elastic piston seals of the new syringes, with which they are then positively connected and without play.
  • the 25 ⁇ l ring locking mechanism is set, 2 x 25 ⁇ l are drawn into a disposable syringe or into a pipette tip placed on the syringe end (turn from one click to the next but one), of which 25 ⁇ l are dispensed into the secondary vessel (simple rest), the rest is discarded with the syringe or pipette tip.
  • the reagents can be stored directly in a twelve-channel pipette designed according to the invention. With this device, one or many times in succession, twelve precisely sized drops of the same size can be dosed in a single operation.
  • the pipette stands in a rack that closes the syringe outlets so that the reagents do not dry out.
  • Deposited particles of a suspension of red blood cells can be whirled up before dosing, e.g. B. by a hollow cylinder made of steel, which is pushed back and forth several times in the syringe with a magnet.
  • a hose with a capacity of over 5 ⁇ l is attached to the end of the syringe.
  • the 5 ⁇ l locking mechanism and the 100 ⁇ l locking mechanism are set (5-% AOI locking mechanism with soft locking, the other is operated by a two-stage push button with harder locking).
  • the syringe is filled with solvent and a volume of up to the first 100 ⁇ l catch is dispensed again.
  • Aspirate 5 ⁇ l air for better separation of the solvent from the sample
  • aspirate 5 ⁇ l sample wipe the end of the tube, or, if a cannula is used, pierce a clean filter paper, dispense 210 ⁇ l into the secondary vessel (the 100 ⁇ l grid will thereby be used) rolled over twice, it clicks into place at the end of the dilution step).
  • a multi-channel dispenser pipette designed according to the invention can be combined with an aspirator.
  • Each channel of the dispenser is connected to a reaction vessel via a hose or a cannula through which it can dispense a certain amount of washing liquid.
  • the liquid is sucked off again by a vacuum over a T-piece of the same hose or over another hose. It is advisable to dispense and aspirate alternately.
  • This method is also suitable for washing flat reaction plates according to ST ⁇ CKER (European patent application 79103987.8). These plates contain reaction fields that are separated from each other by a water-repellent coating. For washing, a second plate made of a water-repellent material is placed at a suitable distance under the reaction plate. Opposite each reaction field, the second plate has a hole through which the multi-channel dispenser dispenses the washing liquid and the aspirator can remove it again.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Feeding, Discharge, Calcimining, Fusing, And Gas-Generation Devices (AREA)
  • Nozzles (AREA)

Claims (8)

1. Dispositif de dosage de liquides pour un prélèvement ou une distribution unique ou répété à intervalles de temps, de volumes dosés, comprenant un boîtier (1502) divisé par une garniture d'étanchéité d'un piston (1504) en deux espaces séparés l'un de l'autre dont l'un est rempli du liquide à doser ou relié à un récipient rempli du liquide à doser, et un système à vis comportant une vis (401; 1516) et un écrou (402; 1518) en prise avec celle-ci, le boîtier étant reliéà la vis et le piston étant relié à l'écrou de la vis ou le boîtier étant relié à l'écrou de la vis et le piston étant relié à la vis, et le piston étant mobile dans le boîtier lorsque la vis est déplacée, caractérisé par un mécanisme à roue crantée qui peut être actionné au moyen d'une roue à main (408; 1508) et/ou d'un levier de commande et qui est rotatif aussi bien dans le sens des aiguilles d'une montre que dans le sens inverse, ledit mécanisme à roue crantée étant relié directement ou indirectement à la vis ou à l'ecrou de la vis, le déplacement du piston étant divisé par ledit mécanisme en intervalles définis dont les longueurs sont proportionnelles à l'angle de rotation formé entre deux crans (roue crantée 413; 1509) ou deux arrêts (1002, 1003) dudit mécanisme.
2. Dispositif de dosage selon la revendication 1 pour le dosage simultané de plusieurs liquides, dans lequel plusieurs boîtiers sont fixés à proximité les uns des autres dans un bloc de boîtiers (1501) et plusieurs pistons sont fixés dans un bloc de pistons (1505), le bloc de boîtiers et le bloc de pistons étant mobiles parallèlement l'un par rapport à l'autre de façon que les pistons lors d'un pas de dosage parcourent, dans tous les boîtiers, une distance exactement égale, caractérisé en ce que la position parallèle du bloc de boîtiers par rapport au bloc de pistons est assurée par un guidage parallèle.
3. Dispositif de dosage selon la revendication 2, caractérisé en ce que le guidage parallèle comporte une ou plusieurs glissières et en ce qu'il est rigide
4. Dispositif de dosage selon la revendication 2, caractérisé en ce que le guidage parallèle est effectué par le mécanisme de déplacement, ledlt mécanisme s'appuyant en plusieurs endroits opposés du bloc de boîtiers et du bloc de pistons, de préférence sur les côtés, et changeant simultanément la distance entre les deux blocs auxdits endroits, avec une valeur égale.
5. Dispositif de dosage selon l'une ou plusieurs des revendications précédentes avec un volume de dosage présélectionné, caractérisé en ce que le volume de dosage peut être présélectionné par la mise en oeuvre de seringues de différentes sections intérieures.
6. Dispositif de dosage selon l'une ou plusieurs des revendications précédentes, avec un volume de dosage présélectionné, caractérisé en ce que le volume de dosage peut être sélectionné par le réglage (1509 ou 1510) de la distance entre les crans ou entre les arrêts (1002; 1003) de façon étagée ou continue.
7. Dispositif de dosage selon l'une ou plusieurs des revendications précédentes, avec un volume de dosage présélectionné, caractérisé en ce que le volume de dosage est présélectionne par le fait que des distances déterminées entre les crans ou entre les arrêts correspondent à des courses différentes des piston dans les boîtiers.
8. Dispositif selon la revendication 7, caraactérisé en ce que la correspondance entre les courses des pistons et les distances déterminées entre les crans ou entre les arrêts s'effectue par une construction en forme de ciseaux dans laquelle les branches des ciseaux sont déplaçables dans le sens longitudinal (814; 815).
EP80105031A 1980-08-25 1980-08-25 Dispositifs de dosage pour liquides ou gaz Expired EP0046461B1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AT80105031T ATE22402T1 (de) 1980-08-25 1980-08-25 Dosiervorrichtungen fuer fluessigkeiten oder gase.
EP80105031A EP0046461B1 (fr) 1980-08-25 1980-08-25 Dispositifs de dosage pour liquides ou gaz
DE8080105031T DE3071776D1 (en) 1980-08-25 1980-08-25 Apparatus for dosing liquids or gases

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP80105031A EP0046461B1 (fr) 1980-08-25 1980-08-25 Dispositifs de dosage pour liquides ou gaz

Publications (2)

Publication Number Publication Date
EP0046461A1 EP0046461A1 (fr) 1982-03-03
EP0046461B1 true EP0046461B1 (fr) 1986-09-24

Family

ID=8186765

Family Applications (1)

Application Number Title Priority Date Filing Date
EP80105031A Expired EP0046461B1 (fr) 1980-08-25 1980-08-25 Dispositifs de dosage pour liquides ou gaz

Country Status (3)

Country Link
EP (1) EP0046461B1 (fr)
AT (1) ATE22402T1 (fr)
DE (1) DE3071776D1 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8617508D0 (en) * 1986-07-17 1986-08-28 Flow Lab Liquid handling station
EP0801982B1 (fr) * 1996-04-18 2002-08-28 Walu Labortechnik GmbH Burette à piston
DE19734599C2 (de) * 1997-08-09 2000-11-23 Hirschmann Laborgeraete Gmbh Mehrkanalige Hubkolbenpipette
DE502004001011D1 (de) * 2003-05-14 2006-09-07 Tecan Trading Ag Vorrichtung zum präzisen Anfahren von Mikroplatten-Wells
FR2920675B1 (fr) * 2007-09-10 2010-12-03 Gilson Sas Systeme de pipetage multicanaux comprenant un porte-pistons a guidage ameliore
WO2010082080A1 (fr) * 2009-01-16 2010-07-22 Gilson S.A.S. Système multi-canaux de guidage de pipettes
FI3928868T3 (fi) * 2020-06-22 2025-02-20 Eppendorf Se Pipetti käytettäväksi männän ja sylinterin käsittävän pipetinkärjen tai ruiskun kanssa
CN113252400A (zh) * 2021-05-13 2021-08-13 西安市红会医院 一种用于生物免疫细胞检测用细胞吸取装置
CN118415816B (zh) * 2024-04-23 2025-07-04 中国人民解放军总医院第六医学中心 一种咽鼓管周间隙进行定量注射材料的套管结构

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2409656A (en) * 1945-04-20 1946-10-22 Harold S Austin Hypodermic syringe
DE866988C (de) * 1946-02-09 1953-02-12 Zbislaw M Roehr Medizinisches Injektionsgeraet
US2695023A (en) * 1952-01-04 1954-11-23 Pfizer & Co C Hypodermic syringe
FR1186571A (fr) * 1957-11-19 1959-08-27 Legris Fils Seringue automatique réglable à doses égales successives
DE2114108A1 (de) * 1970-05-15 1971-11-25 Ollituote Oy Pipettenspitzenreihe,insbesondere zur maschinellen Fluessigkeitsdosierung
DE2233913A1 (de) * 1971-07-22 1973-02-01 Instrumentation Labor Inc Dosiervorrichtung
US3863805A (en) * 1972-06-08 1975-02-04 Lkb Produkter Ab Exchangeable piston pump unit
DE2439269A1 (de) * 1974-08-16 1976-02-26 Dean Kamen Arzneimittel-injektionseinrichtung
DE2731536A1 (de) * 1976-07-09 1978-03-09 Le Dev De L Automatisme En Bio Geraet zum untersuchen der kleinstmengen von fluessigkeiten in groesserer zahl mit fluessigen reaktionsstoffen
CH607620A5 (fr) * 1977-03-07 1978-09-29 Contraves Ag
DE2752073A1 (de) * 1977-11-22 1979-05-23 Hettich Andreas Fa Vorrichtung zur gleichzeitigen abgabe dosierter fluessigkeitsmengen zur medizinischen verwendung

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI50813C (fi) * 1970-05-12 1976-07-12 Matti Antero Reunanen Menetelmä ja laite reagenssien annostelua, niiden inkubointia ja reakt ioseoksen näytteenottoa varten.

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2409656A (en) * 1945-04-20 1946-10-22 Harold S Austin Hypodermic syringe
DE866988C (de) * 1946-02-09 1953-02-12 Zbislaw M Roehr Medizinisches Injektionsgeraet
US2695023A (en) * 1952-01-04 1954-11-23 Pfizer & Co C Hypodermic syringe
FR1186571A (fr) * 1957-11-19 1959-08-27 Legris Fils Seringue automatique réglable à doses égales successives
DE2114108A1 (de) * 1970-05-15 1971-11-25 Ollituote Oy Pipettenspitzenreihe,insbesondere zur maschinellen Fluessigkeitsdosierung
DE2233913A1 (de) * 1971-07-22 1973-02-01 Instrumentation Labor Inc Dosiervorrichtung
US3863805A (en) * 1972-06-08 1975-02-04 Lkb Produkter Ab Exchangeable piston pump unit
DE2439269A1 (de) * 1974-08-16 1976-02-26 Dean Kamen Arzneimittel-injektionseinrichtung
DE2731536A1 (de) * 1976-07-09 1978-03-09 Le Dev De L Automatisme En Bio Geraet zum untersuchen der kleinstmengen von fluessigkeiten in groesserer zahl mit fluessigen reaktionsstoffen
CH607620A5 (fr) * 1977-03-07 1978-09-29 Contraves Ag
DE2752073A1 (de) * 1977-11-22 1979-05-23 Hettich Andreas Fa Vorrichtung zur gleichzeitigen abgabe dosierter fluessigkeitsmengen zur medizinischen verwendung

Also Published As

Publication number Publication date
ATE22402T1 (de) 1986-10-15
EP0046461A1 (fr) 1982-03-03
DE3071776D1 (en) 1986-10-30

Similar Documents

Publication Publication Date Title
DE69835691T2 (de) Vielkanal-pipettiergerät
DE7925075U1 (de) Pipettier- und dosiervorrichtung
DE3781762T2 (de) Mehrkanal-pipette.
DE69516134T2 (de) Vorrichtung und verfahren für eine hochparallele pipette
DE7330354U (de) Mit der hand zu haltende pipette
DE2402166C3 (de) Anordnung zur automatischen Analyse von Flüssigkeitsproben
EP2633913B1 (fr) Dispositif de pipetage et dispositif de pipetage multi-canal
EP0649678A1 (fr) Pipette à piston
DE2120719A1 (de) Pipettier-Anordnung
EP1177831B1 (fr) Dispositif à actionnement manuel pour distribuer et/ou prélever des liquides
DE1498960B2 (fr)
DE2640491A1 (de) Automatischer pipettierapparat
EP0046461B1 (fr) Dispositifs de dosage pour liquides ou gaz
DE2720669B2 (de) Vorrichtung zum Vermischen einer flüssigen Probe mit einer Verdünnungsflüssigkeit
DE3701250A1 (de) Dosierpipette
CH617362A5 (fr)
DE69921051T2 (de) Saugvorrichtung zur Abgabe von Flüssigkeiten
DE2808068A1 (de) Handpipette
DE2825015C2 (de) Flüssigkeitsausgabegerät
EP3851191A1 (fr) Procédé de fonctionnement d'une pipette à course de piston, pipette à course de piston, appareil et système de traitement des données
DE4104831A1 (de) Dosierelement
WO1982004409A1 (fr) Dispositif pour prelever une quantite determinee de liquide d'un recipient
DE1598332A1 (de) Gasspuergeraet
DE3216644A1 (de) Wiederholspender
DD141357A5 (de) Vorrichtung zur erzielung einer lagenverseilung auf doppelschlagmaschinen

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

AK Designated contracting states

Designated state(s): AT BE CH DE FR GB IT LU NL SE

17P Request for examination filed

Effective date: 19820830

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: STOECKER, WINFRIED, DR. MED.

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AT BE CH DE FR GB IT LI LU NL SE

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: NL

Effective date: 19860924

Ref country code: IT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT;WARNING: LAPSES OF ITALIAN PATENTS WITH EFFECTIVE DATE BEFORE 2007 MAY HAVE OCCURRED AT ANY TIME BEFORE 2007. THE CORRECT EFFECTIVE DATE MAY BE DIFFERENT FROM THE ONE RECORDED.

Effective date: 19860924

Ref country code: BE

Effective date: 19860924

REF Corresponds to:

Ref document number: 22402

Country of ref document: AT

Date of ref document: 19861015

Kind code of ref document: T

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SE

Effective date: 19860930

REF Corresponds to:

Ref document number: 3071776

Country of ref document: DE

Date of ref document: 19861030

ET Fr: translation filed
NLV1 Nl: lapsed or annulled due to failure to fulfill the requirements of art. 29p and 29m of the patents act
PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: AT

Effective date: 19870825

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LU

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 19870831

Ref country code: LI

Effective date: 19870831

Ref country code: CH

Effective date: 19870831

26N No opposition filed
REG Reference to a national code

Ref country code: CH

Ref legal event code: PL

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: GB

Payment date: 19910730

Year of fee payment: 12

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: FR

Payment date: 19910830

Year of fee payment: 12

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: GB

Effective date: 19920825

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: DE

Payment date: 19921019

Year of fee payment: 13

GBPC Gb: european patent ceased through non-payment of renewal fee

Effective date: 19920825

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: FR

Effective date: 19930430

REG Reference to a national code

Ref country code: FR

Ref legal event code: ST

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: DE

Effective date: 19940503