EP0436585A1 - Conservation de la viande - Google Patents
Conservation de la viandeInfo
- Publication number
- EP0436585A1 EP0436585A1 EP89910589A EP89910589A EP0436585A1 EP 0436585 A1 EP0436585 A1 EP 0436585A1 EP 89910589 A EP89910589 A EP 89910589A EP 89910589 A EP89910589 A EP 89910589A EP 0436585 A1 EP0436585 A1 EP 0436585A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- acetic acid
- meat
- solid material
- sodium diacetate
- substance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 235000013372 meat Nutrition 0.000 title claims abstract description 34
- 238000004321 preservation Methods 0.000 title description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 75
- 238000000034 method Methods 0.000 claims abstract description 38
- 239000001632 sodium acetate Substances 0.000 claims abstract description 27
- 239000000126 substance Substances 0.000 claims abstract description 16
- 235000017454 sodium diacetate Nutrition 0.000 claims description 23
- OPGYRRGJRBEUFK-UHFFFAOYSA-L disodium;diacetate Chemical compound [Na+].[Na+].CC([O-])=O.CC([O-])=O OPGYRRGJRBEUFK-UHFFFAOYSA-L 0.000 claims description 22
- 239000011343 solid material Substances 0.000 claims description 20
- 239000000463 material Substances 0.000 claims description 11
- 159000000021 acetate salts Chemical class 0.000 claims description 3
- 241000251468 Actinopterygii Species 0.000 abstract description 4
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 abstract description 4
- 235000015278 beef Nutrition 0.000 abstract description 4
- 235000015277 pork Nutrition 0.000 abstract description 4
- 235000017281 sodium acetate Nutrition 0.000 abstract description 4
- 244000005700 microbiome Species 0.000 abstract description 3
- 244000144977 poultry Species 0.000 abstract description 3
- 235000013594 poultry meat Nutrition 0.000 abstract description 3
- 239000007787 solid Substances 0.000 abstract description 3
- 241000238424 Crustacea Species 0.000 abstract description 2
- 150000001875 compounds Chemical class 0.000 abstract description 2
- 238000005507 spraying Methods 0.000 abstract description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 abstract 1
- 150000001242 acetic acid derivatives Chemical class 0.000 abstract 1
- 238000007590 electrostatic spraying Methods 0.000 abstract 1
- 235000019688 fish Nutrition 0.000 abstract 1
- 238000010561 standard procedure Methods 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 12
- 229910001868 water Inorganic materials 0.000 description 12
- 241001465754 Metazoa Species 0.000 description 6
- 230000008901 benefit Effects 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 241000607142 Salmonella Species 0.000 description 4
- 241000287828 Gallus gallus Species 0.000 description 3
- 235000013330 chicken meat Nutrition 0.000 description 3
- 239000011248 coating agent Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 238000004090 dissolution Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 235000013622 meat product Nutrition 0.000 description 3
- 230000002906 microbiologic effect Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000007921 spray Substances 0.000 description 3
- 239000000052 vinegar Substances 0.000 description 3
- 235000021419 vinegar Nutrition 0.000 description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 238000010410 dusting Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000013373 food additive Nutrition 0.000 description 2
- 239000002778 food additive Substances 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 241000894007 species Species 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 241000305071 Enterobacterales Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 235000019687 Lamb Nutrition 0.000 description 1
- 241000353345 Odontesthes regia Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000004599 antimicrobial Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 239000006172 buffering agent Substances 0.000 description 1
- 150000001669 calcium Chemical class 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 159000000007 calcium salts Chemical class 0.000 description 1
- 239000000378 calcium silicate Substances 0.000 description 1
- 229910052918 calcium silicate Inorganic materials 0.000 description 1
- OYACROKNLOSFPA-UHFFFAOYSA-N calcium;dioxido(oxo)silane Chemical compound [Ca+2].[O-][Si]([O-])=O OYACROKNLOSFPA-UHFFFAOYSA-N 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 235000013351 cheese Nutrition 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000009470 controlled atmosphere packaging Methods 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000009503 electrostatic coating Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 210000000416 exudates and transudate Anatomy 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 231100001261 hazardous Toxicity 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 235000020429 malt syrup Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000008239 natural water Substances 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 238000005554 pickling Methods 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 235000019684 potato crisps Nutrition 0.000 description 1
- 229920003133 pregelled starch Polymers 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000013580 sausages Nutrition 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 239000004460 silage Substances 0.000 description 1
- 238000003307 slaughter Methods 0.000 description 1
- 235000011888 snacks Nutrition 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- WSWCOQWTEOXDQX-MQQKCMAXSA-N sorbic acid group Chemical class C(\C=C\C=C\C)(=O)O WSWCOQWTEOXDQX-MQQKCMAXSA-N 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 229940078499 tricalcium phosphate Drugs 0.000 description 1
- 229910000391 tricalcium phosphate Inorganic materials 0.000 description 1
- 235000019731 tricalcium phosphate Nutrition 0.000 description 1
- 238000009461 vacuum packaging Methods 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
- A23B4/00—Preservation of meat, sausages, fish or fish products
- A23B4/10—Coating with a protective layer; Compositions or apparatus therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
- A23B4/00—Preservation of meat, sausages, fish or fish products
- A23B4/12—Preserving with acids; Acid fermentation
Definitions
- This Invention relates to the preservation of meat products.
- CSIRO Scientific and Industrial Research Organisation
- 1t utilises acetic acid washing to increase the shelf Hfe of chilled fresh vacuum-packed beef and sheep carcasses.
- the method Involves treating the carcass at 55°C with 1.5% acetic add and results in an increase of approximately 50% in the shelf-Hfe of the products. This procedure has been accepted by local exporters and foreign importers, and approval for its application with respect to the United States has been given via appropriate food regulations .
- the disadvantages of this method include the need to operate at higher than normal ambient temperatures and the requirement for solution treatment of the carcass with resultant requirement for constant monitoring of the acetic acid concentration to ensure that carcass water and exudates do not dilute 1t and thereby reduce its effectiveness.
- meat in the context of this invention refers to any animal flesh suitable for human consumption and 1s considered to Include not only beef, mutton, lamb and pork but also poultry or game birds and marine animals such as fish, shellfish and crustaceans.
- the solid material is one which will liberate acetic acid in the presence of moisture.
- One well-known substance which will do this 1s sodium diacetate which on complete dissolution in water is present as a mixture of sodium acetate and acetic add.
- This substance 1s already known and used as a food additive For example, rope and/or mould in bread, cheese and malt syrups can be controlled by the addition of sodium diacetate to the foodstuff concerned.
- the use of this substance for grain and silage preservation has also been proposed, as has its use to improve the palatability of animal feed.
- a further food-related use is the use to provide the vinegar flavour in various snack foods such as "salt and vinegar"-flavoured potato crisps.
- sodium diacetate is known as an additive in some sausage meats (see, for example, "Ordinance on amendment of meat ordinance” ("Verix Ober And réelle der Fleisch-Verowski” ) of 25th August, 1969 by the Bundesminister fur tranquiloire, Federal Republic of Germany as reported 1n the "Archiv fur Lebensm1ttelhyg1ene” 20(9) (September 1969) at p.213).
- Sodium diacetate is preferred for use in this invention as 1t has the advantage of already being a recognised food additive. It has been declared “generally regarded as safe” by the United States regulatory authorities and 1s exempted there from residue tolerance limits.
- Substances other than sodium diacetate which release acetic add on exposure to moisture are also useful in this invention. These substances are, like sodium diacetate, "complexes" of monovalent and divalent acetate salts and acetic acid, wherein the acetic acid is believed to be held in the molecule in a manner analogous to the water of crystallisation in a crystalline hydrated salt. On dissolution, the "complex” readily dissociates and, in the case of sodium diacetate, the result 1s a solution containing sodium acetate and acetic acid.
- the acetic add is present in an approximately equimolar mixture of undissodated and dissociated species due to the production of a buffered medium at a pH approximately equal to the p a of acetic acid.
- the acetic acid concentration can be very high.
- the pH of the buffered medium being different, depending on the nature of the acetate salt.
- typical substances useful in the working of this Invention include the ammonium, potassium and calcium analogues of sodium diacetate.
- the meat to be coated is preferably substantially dry, by which is meant that no water drips fro ⁇ the hung meat. Many meat products are washed with water prior to storage for transport and/or later use and in such cases, it 1s desirable to remove excess superficial water from the meat, for example, by blowing the water from the meat surface.
- Minimal water on the meat surface provides a better environment for microbial control in that, when the solid material 1s coated thereon, there is produced thereon a very effective concentrated mixture of species: in the case of sodium diacetate there 1s sodium acetate (acting as a buffering agent), acetic add (1n both the molecular and dissociated forms), and sodium diacetate molecules (in the process of dissolution).
- the natural water content of the meat generally provides sufficient moisture to release acetic acid from the solid material .
- the solid material may be applied to the meat in any suitable manner, preferably so as to provide a substantially uniform fine coating.
- electrostatic coating equipment can be adapted to the application of the present dry material to the meat surface. This is a highly satisfactory method.
- the geometry of the spraying arrangement is such that the inner cavity of the carcasses receives spray.
- a dusting box may be used in which the solid material 1s introduced and maintained in suspension during the residence of the meat within the box.
- the extent of coating may be gauged by any suitable means.
- An especially suitable apparatus is described 1n co-pending Australian patent application No. PJ3940.
- sodium diacetate In the case of sodium diacetate, this means that the sodium diacetate should be applied to the extent that the meat receives fro ⁇ 0.5 -5.0 mg solid mater1al/sq cm. Preferably, sodium diacetate should be applied at the rate of from 1-3 mg/sq cm.
- the solid material applied to the meat may be entirely composed of the acetic acid-releasing substance or it may optionally contain other materials, either inert materials as diluents or materials which alter in some way the properties of the overall material. Typical materials are free-flow agents such as tricalcium phosphate and calcium silicate, water activity reducing agents such as pre-gelled starch and complementary antimicrobial agents such as the sodium, potassium or calcium salts of sorbic or propionlc acid.
- 1t 1s preferable that the total coating material should be comprised of at least 25% by weight of the substance. It is possible to use less than 25%, but this requires that a very large quantity of material must be applied to achieve the desired effect and this has considerable practical drawbacks.
- the method provided by the present Invention has numerous advantages over prior art methods used to extend the shelf-life of meat products.
- the present method 1 s compatible with the further processing of ⁇ eat products as currently used such as vacuum and controlled atmosphere packaging.
- fie current cost of sodium diacetate, coupled with Its rate of use makes the method of this invention economically attractive. This 1s particularly so when the simple application technology can be combined with such complementary technologies as specialised packaging systems.
- This example describes the use of sodium diacetate on beef.
- Substantially dry chicken carcasses from which all Internal organs had been removed were suspended by the legs and their exterior surfaces sprayed with sodium diacetate by means of electrostatic spray equip entto give a level of about 3 mg/sq cm.
- the carcasses were stored at 2°C and were subjected to regular microbiological testing, there also being included a number of similar carcasses which had not been sprayed.
- Substantially dry rock ⁇ ng fish fillets obtained commercially were dusted with sodium diacetate from a vibrating 45 um sieve to a level of about 3 mg/sq.cm. These fillets were sealed 1n Stomacher bags as were a number of control (untreated but otherwise similar) fillets. Regular plate counts were taken and these revealed that the control samples had an unacceptable level of microorganisms after 3 days with a standard plate count in excess of 10 counts/g; while after 18 days, the treated fillets standard plate count had still not reached 10 counts/g.
- Slices of pork were treated with sodium diacetate from a vibrating 45 um sieve at two rates, about 1 mg/sq cm and about 3 mg/sq cm. These along with similar but untreated controls were kept in commercial polystyrene trays covered with clear plastics film at 2 C and standard plate counts were taken at regular Intervals. The control samples were unacceptable after 11 days whereas the treated meat (1 mg/sq.cm) samples were unacceptable after 21 days and the treated (3 mg/sq.cm.) samples were still microbiologically acceptable after 23 days.
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Meat, Egg Or Seafood Products (AREA)
Abstract
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AUPJ068588 | 1988-09-29 | ||
| AU685/88 | 1988-09-29 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EP0436585A1 true EP0436585A1 (fr) | 1991-07-17 |
| EP0436585A4 EP0436585A4 (en) | 1992-01-02 |
Family
ID=3773408
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP19890910589 Ceased EP0436585A4 (en) | 1988-09-29 | 1989-09-19 | Meat preservation |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP0436585A4 (fr) |
| KR (1) | KR900701170A (fr) |
| DK (1) | DK46991A (fr) |
| ES (1) | ES2018733A6 (fr) |
| GR (1) | GR890100614A (fr) |
| NZ (1) | NZ230807A (fr) |
| PT (1) | PT91837A (fr) |
| WO (1) | WO1990003118A1 (fr) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PT95787A (pt) * | 1989-11-06 | 1992-07-31 | Haig Frederick David | Dispositivo de distribuicao de materiais em po |
| JP2002512054A (ja) * | 1998-04-30 | 2002-04-23 | エフ.グラベ エルマー | 鶏肉製品 |
| NL1010673C2 (nl) * | 1998-11-27 | 2000-05-30 | Stork Pmt | Toevoegen van additief aan een vleesprodukt. |
| WO2002082914A1 (fr) * | 2001-04-13 | 2002-10-24 | Glabe Elmer F | Produit de poisson frais conditionne et procede de preparation |
| US20030118704A1 (en) * | 2001-12-11 | 2003-06-26 | Purac Biochem B.V. | Process for preserving food products |
| CA2793020C (fr) * | 2005-09-15 | 2015-11-24 | Triad Resource Technologies, Llc | Compositions destinees a ameliorer la saveur et la securite de viandes marinees |
| EP3114205A4 (fr) * | 2014-03-06 | 2017-11-29 | Kemin Industries, Inc. | Compositions antimicrobiennes pour produits de viande |
| CN110367326A (zh) * | 2019-08-23 | 2019-10-25 | 南京工业大学 | 一种肉制品生物复合保鲜剂及其保鲜方法 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB664845A (en) * | 1949-02-24 | 1952-01-16 | Dow Chemical Co | An improved method for rendering proteinaceous and fatty foods resistant to microorganisms |
| US2856294A (en) * | 1954-05-28 | 1958-10-14 | Best Foods Inc | Mold inhibiting processes and products thereof |
| AU283653B2 (en) * | 1963-09-17 | 1968-06-11 | The Griffith Laboratories Inc | Method of producing a meat curing salt composition |
| CH468792A (de) * | 1965-08-25 | 1969-02-28 | Peter Dr Flesch | Zusatzmittel für die Herstellung von Brühwurst |
| JPS53116315A (en) * | 1977-03-17 | 1978-10-11 | Ueno Seiyaku Oyo Kenkyujo Kk | Powder or granular containing improved sorbinic acid |
| GB2000430B (en) * | 1977-07-01 | 1982-02-17 | Broeste Ind As | Method for producing smoke-flavoured fish |
| DE2925821A1 (de) * | 1979-06-27 | 1981-01-29 | Wolfgang Prof Dr Thiel | Verfahren zur haltbarmachung von frischfleisch und tauchloesung zur durchfuehrung des verfahrens |
| JPS5922508B2 (ja) * | 1979-09-25 | 1984-05-26 | 成和化成株式会社 | 食品の凍結方法 |
| EP0099716A1 (fr) * | 1982-07-16 | 1984-02-01 | BP Chemicals Limited | Fabrication de produits de protéine moulés |
| JPS5959182A (ja) * | 1982-09-27 | 1984-04-04 | Tomoji Tanaka | 生魚畜肉等の鮮度保持と酸化防止 |
| DE3637840A1 (de) * | 1986-11-06 | 1988-05-19 | Schuelke & Mayr Gmbh | Wursttauchmasse |
| US4770884A (en) * | 1987-09-18 | 1988-09-13 | Monsanto Company | Control of Salmonella on poultry carcasses |
| FR2788775B1 (fr) * | 1999-01-22 | 2001-04-13 | Pf Medicament | Nouvelles n-alcoyl-n-[1-(omega-(arylalcoyloxy)alcoyl] piperidin-4-yl]-4h-3,1-benzo(thia/oxa)zines-2-amines substituees, leur preparation et leur application en therapeutique |
-
1989
- 1989-09-19 EP EP19890910589 patent/EP0436585A4/en not_active Ceased
- 1989-09-19 KR KR1019900701081A patent/KR900701170A/ko not_active Withdrawn
- 1989-09-19 WO PCT/AU1989/000405 patent/WO1990003118A1/fr not_active Ceased
- 1989-09-27 NZ NZ230807A patent/NZ230807A/xx unknown
- 1989-09-28 PT PT91837A patent/PT91837A/pt unknown
- 1989-09-28 GR GR890100614A patent/GR890100614A/el unknown
- 1989-09-29 ES ES8903298A patent/ES2018733A6/es not_active Expired - Fee Related
-
1991
- 1991-03-15 DK DK046991A patent/DK46991A/da not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| PT91837A (pt) | 1990-04-30 |
| DK46991D0 (da) | 1991-03-15 |
| ES2018733A6 (es) | 1991-05-01 |
| WO1990003118A1 (fr) | 1990-04-05 |
| EP0436585A4 (en) | 1992-01-02 |
| GR890100614A (el) | 1990-10-31 |
| DK46991A (da) | 1991-03-15 |
| KR900701170A (ko) | 1990-12-01 |
| NZ230807A (en) | 1993-03-26 |
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Legal Events
| Date | Code | Title | Description |
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| PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
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| 17P | Request for examination filed |
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Free format text: STATUS: THE APPLICATION HAS BEEN REFUSED |
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| 18R | Application refused |
Effective date: 19940508 |