[go: up one dir, main page]

WO2024236009A3 - Nouvelles estérases et leurs utilisations - Google Patents

Nouvelles estérases et leurs utilisations Download PDF

Info

Publication number
WO2024236009A3
WO2024236009A3 PCT/EP2024/063321 EP2024063321W WO2024236009A3 WO 2024236009 A3 WO2024236009 A3 WO 2024236009A3 EP 2024063321 W EP2024063321 W EP 2024063321W WO 2024236009 A3 WO2024236009 A3 WO 2024236009A3
Authority
WO
WIPO (PCT)
Prior art keywords
esterases
seq
polyethylene terephthalate
acidic conditions
esterase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/EP2024/063321
Other languages
English (en)
Other versions
WO2024236009A2 (fr
Inventor
Isabelle ANDRÉ
Grégory ARNAL
Nicolas Chabot
Marc GUÉROULT
Guy Lippens
Alain Marty
Alexandra TAUZIN
Vincent TOURNIER
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Carbios SA
Original Assignee
Carbios SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carbios SA filed Critical Carbios SA
Publication of WO2024236009A2 publication Critical patent/WO2024236009A2/fr
Publication of WO2024236009A3 publication Critical patent/WO2024236009A3/fr
Anticipated expiration legal-status Critical
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J11/00—Recovery or working-up of waste materials
    • C08J11/04—Recovery or working-up of waste materials of polymers
    • C08J11/10—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
    • C08J11/105—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation by treatment with enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01074—Cutinase (3.1.1.74)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
    • C08J2367/02—Polyesters derived from dicarboxylic acids and dihydroxy compounds
    • C08J2367/03—Polyesters derived from dicarboxylic acids and dihydroxy compounds the dicarboxylic acids and dihydroxy compounds having the hydroxy and the carboxyl groups directly linked to aromatic rings

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Polymers & Plastics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sustainable Development (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)

Abstract

La présente invention concerne de nouvelles estérases, plus particulièrement des variants d'estérase ayant une activité améliorée et/ou une thermostabilité améliorée par rapport à l'estérase parente de SEQ ID NO : 1 ou de SEQ ID NO : 2 dans des conditions acides, ainsi que leurs utilisations pour dégrader un matériau contenant du polyester, tel que les produits en plastique. Les estérases de l'invention sont particulièrement appropriées pour dégrader le polyéthylène téréphtalate, et un matériau contenant du polyéthylène téréphtalate dans des conditions acides.
PCT/EP2024/063321 2023-05-15 2024-05-15 Nouvelles estérases et leurs utilisations Pending WO2024236009A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP23305770 2023-05-15
EP23305770.2 2023-05-15

Publications (2)

Publication Number Publication Date
WO2024236009A2 WO2024236009A2 (fr) 2024-11-21
WO2024236009A3 true WO2024236009A3 (fr) 2025-01-09

Family

ID=86604932

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2024/063321 Pending WO2024236009A2 (fr) 2023-05-15 2024-05-15 Nouvelles estérases et leurs utilisations

Country Status (2)

Country Link
TW (1) TW202511480A (fr)
WO (1) WO2024236009A2 (fr)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018011281A1 (fr) * 2016-07-12 2018-01-18 Carbios Nouvelles estérases et utilisations associées
WO2020021116A1 (fr) * 2018-07-27 2020-01-30 Carbios Nouvelles estérases et leurs utilisations
WO2020021117A1 (fr) * 2018-07-27 2020-01-30 Carbios Nouvelles estérases et leurs utilisations
WO2022243545A1 (fr) * 2021-05-20 2022-11-24 Carbios Procédé de dégradation d'un produit plastique comprenant au moins un polyester
WO2023019222A1 (fr) * 2021-08-11 2023-02-16 Biometis Technology, Inc. Dégradation enzymatique de polyéthylène téréphtalate

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2984354A1 (fr) 2011-12-20 2013-06-21 Centre Nat Rech Scient Procede de preparation d'alliage polymere/enzymes
US10124512B2 (en) 2012-11-20 2018-11-13 Carbios Method for recycling plastic products
CA2947478C (fr) 2014-05-16 2022-09-13 Carbios Procede de recyclage d'articles de plastique de polyethylene terephtalate mixtes
US10723848B2 (en) 2015-06-12 2020-07-28 Carbios Masterbatch composition comprising a high concentration of biological entities
CA3024287C (fr) 2016-05-19 2024-06-11 Carbios Procede de degradation de produits plastiques
KR102649944B1 (ko) 2016-07-12 2024-03-22 까르비오 신규한 에스테라아제 및 그의 용도
CA3073791A1 (fr) 2017-08-31 2019-03-07 Carbiolice Article en polyester biodegradable comprenant des enzymes
MX2020002290A (es) 2017-08-31 2020-07-13 Carbios Composicion liquida que comprende entidades biologicas y usos de la misma.
US11851689B2 (en) 2018-07-27 2023-12-26 Carbios Esterases and uses thereof
FR3088070B1 (fr) 2018-11-06 2021-11-26 Carbios Procede de degradation enzymatique de polyethylene terephtalate
FR3088069B1 (fr) 2018-11-06 2021-11-26 Carbios Procede de production d'acide terephtalique a l'echelle industrielle
EP3838976B1 (fr) 2019-12-19 2025-02-12 Carbios Procédé de dégradation de produits plastiques
MX2022007496A (es) 2019-12-19 2022-07-01 Carbios Proceso para degradar productos plasticos.
CN117866280A (zh) 2019-12-19 2024-04-12 卡比奥斯公司 发泡塑料组合物

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018011281A1 (fr) * 2016-07-12 2018-01-18 Carbios Nouvelles estérases et utilisations associées
WO2020021116A1 (fr) * 2018-07-27 2020-01-30 Carbios Nouvelles estérases et leurs utilisations
WO2020021117A1 (fr) * 2018-07-27 2020-01-30 Carbios Nouvelles estérases et leurs utilisations
WO2022243545A1 (fr) * 2021-05-20 2022-11-24 Carbios Procédé de dégradation d'un produit plastique comprenant au moins un polyester
WO2023019222A1 (fr) * 2021-08-11 2023-02-16 Biometis Technology, Inc. Dégradation enzymatique de polyéthylène téréphtalate

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
DATABASE Geneseq [online] 19 March 2020 (2020-03-19), "Metagenome-derived cutinase variant (V17).", retrieved from EBI accession no. GSP:BHG01932 Database accession no. BHG01932 *
DATABASE Geneseq [online] 19 March 2020 (2020-03-19), "Metagenome-derived cutinase variant (V26).", retrieved from EBI accession no. GSP:BHG01941 Database accession no. BHG01941 *
DATABASE Geneseq [online] 19 March 2020 (2020-03-19), "Mutant esterase protein, SEQ ID NO:2.", retrieved from EBI accession no. GSP:BHG01024 Database accession no. BHG01024 *
DATABASE Geneseq [online] 22 December 2022 (2022-12-22), "PET degrading mutant cutinase enzyme F208I/D203C/S248C/V170I/Y92G.", retrieved from EBI accession no. GSP:BLZ66933 Database accession no. BLZ66933 *
DATABASE Geneseq [online] 23 March 2023 (2023-03-23), "Bhr-PETase S13L.", retrieved from EBI accession no. GSP:BML88009 Database accession no. BML88009 *
DATABASE Geneseq [online] 5 August 2021 (2021-08-05), "Cutinase protein mutant F208I/D203C/S248C/V170I/Y92G.", retrieved from EBI accession no. GSP:BJN91066 Database accession no. BJN91066 *
URBANEK ANETA K ET AL: "Biochemical properties and biotechnological applications of microbial enzymes involved in the degradation of polyester-type plastics", BIOCHIMICA ET BIOPHYSICA ACTA (BBA) - PROTEINS & PROTEOMICS, ELSEVIER, NETHERLANDS, vol. 1868, no. 2, 16 November 2019 (2019-11-16), XP086038547, ISSN: 1570-9639, [retrieved on 20191116], DOI: 10.1016/J.BBAPAP.2019.140315 *

Also Published As

Publication number Publication date
TW202511480A (zh) 2025-03-16
WO2024236009A2 (fr) 2024-11-21

Similar Documents

Publication Publication Date Title
EP4424823A3 (fr) Nouvelles estérases et leurs utilisations
MX2021001045A (es) Nuevas esterasas y usos de las mismas.
MX2022000469A (es) Esterasas y usos de las mismas.
WO2023088909A3 (fr) Nouvelles estérases et leurs utilisations
MX2023011423A (es) Nuevas esterasas y usos de las mismas.
MX2019000493A (es) Nuevas esterasas y usos de las mismas.
Urbanek et al. Current knowledge on polyethylene terephthalate degradation by genetically modified microorganisms
Tournier et al. Enzymes’ power for plastics degradation
Muroi et al. Characterization of a poly (butylene adipate-co-terephthalate) hydrolase from the aerobic mesophilic bacterium Bacillus pumilus
Liu et al. Efficient enzymatic degradation of poly (ɛ-caprolactone) by an engineered bifunctional lipase-cutinase
Hu et al. Enzymatic degradation of poly (butylene succinate) by cutinase cloned from Fusarium solani
Roohi et al. PHB (poly‐β‐hydroxybutyrate) and its enzymatic degradation
Shah et al. Microbial degradation of aliphatic and aliphatic-aromatic co-polyesters
Nair et al. Biodegradation of biopolymers
Puspitasari et al. Class I hydrophobins pretreatment stimulates PETase for monomers recycling of waste PETs
Akutsu-Shigeno et al. Cloning and sequencing of a poly (DL-lactic acid) depolymerase gene from Paenibacillus amylolyticus strain TB-13 and its functional expression in Escherichia coli
Müller et al. Enzymatic degradation of poly (ethylene terephthalate): rapid hydrolyse using a hydrolase from T. fusca
Shirke et al. Toward rational thermostabilization of Aspergillus oryzae cutinase: insights into catalytic and structural stability
Sui et al. Recent advances in the biodegradation of polyethylene terephthalate with cutinase-like enzymes
Gowdhaman et al. Xylanase production from Bacillus aerophilus KGJ2 and its application in xylooligosaccharides preparation
JP6315978B2 (ja) 新規なクチナーゼ、クチナーゼをコードする遺伝子、及びクチナーゼを用いたポリエステル又はエステル化合物の分解方法
BR112012028466A2 (pt) produtos destinados ao consumidor com variantes de protease
TW200700495A (en) Polyester compositions containing high amounts of cyclobutanediol and articles made therefrom
MX2024005891A (es) Esterasas y usos de las mismas.
Driss et al. Production and in vitro evaluation of xylooligosaccharides generated from corncobs using immobilized Penicillium occitanis xylanase

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 24727221

Country of ref document: EP

Kind code of ref document: A2

WWE Wipo information: entry into national phase

Ref document number: 2501007690

Country of ref document: TH