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WO2024236009A3 - Novel esterases and uses thereof - Google Patents

Novel esterases and uses thereof Download PDF

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Publication number
WO2024236009A3
WO2024236009A3 PCT/EP2024/063321 EP2024063321W WO2024236009A3 WO 2024236009 A3 WO2024236009 A3 WO 2024236009A3 EP 2024063321 W EP2024063321 W EP 2024063321W WO 2024236009 A3 WO2024236009 A3 WO 2024236009A3
Authority
WO
WIPO (PCT)
Prior art keywords
esterases
seq
polyethylene terephthalate
acidic conditions
esterase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/EP2024/063321
Other languages
French (fr)
Other versions
WO2024236009A2 (en
Inventor
Isabelle ANDRÉ
Grégory ARNAL
Nicolas Chabot
Marc GUÉROULT
Guy Lippens
Alain Marty
Alexandra TAUZIN
Vincent TOURNIER
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Carbios SA
Original Assignee
Carbios SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carbios SA filed Critical Carbios SA
Publication of WO2024236009A2 publication Critical patent/WO2024236009A2/en
Publication of WO2024236009A3 publication Critical patent/WO2024236009A3/en
Anticipated expiration legal-status Critical
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J11/00—Recovery or working-up of waste materials
    • C08J11/04—Recovery or working-up of waste materials of polymers
    • C08J11/10—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
    • C08J11/105—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation by treatment with enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01074—Cutinase (3.1.1.74)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
    • C08J2367/02—Polyesters derived from dicarboxylic acids and dihydroxy compounds
    • C08J2367/03—Polyesters derived from dicarboxylic acids and dihydroxy compounds the dicarboxylic acids and dihydroxy compounds having the hydroxy and the carboxyl groups directly linked to aromatic rings

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Polymers & Plastics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sustainable Development (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)

Abstract

The present invention relates to novel esterases, more particularly to esterase variants having improved activity and/or improved thermostability compared to the parent esterase of SEQ ID NO:1 or SEQ ID NO:2 in acidic conditions and the uses thereof for degrading polyester containing material, such as plastic products. The esterases of the invention are particularly suited to degrade polyethylene terephthalate, and material containing polyethylene terephthalate in acidic conditions.
PCT/EP2024/063321 2023-05-15 2024-05-15 Novel esterases and uses thereof Pending WO2024236009A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP23305770 2023-05-15
EP23305770.2 2023-05-15

Publications (2)

Publication Number Publication Date
WO2024236009A2 WO2024236009A2 (en) 2024-11-21
WO2024236009A3 true WO2024236009A3 (en) 2025-01-09

Family

ID=86604932

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2024/063321 Pending WO2024236009A2 (en) 2023-05-15 2024-05-15 Novel esterases and uses thereof

Country Status (2)

Country Link
TW (1) TW202511480A (en)
WO (1) WO2024236009A2 (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018011281A1 (en) * 2016-07-12 2018-01-18 Carbios Novel esterases and uses thereof
WO2020021116A1 (en) * 2018-07-27 2020-01-30 Carbios Novel esterases and uses thereof
WO2020021117A1 (en) * 2018-07-27 2020-01-30 Carbios Novel esterases and uses thereof
WO2022243545A1 (en) * 2021-05-20 2022-11-24 Carbios Process for degrading a plastic product comprising at least one polyester
WO2023019222A1 (en) * 2021-08-11 2023-02-16 Biometis Technology, Inc. Enzymatic degradation of polyethylene terephthalate

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2984354A1 (en) 2011-12-20 2013-06-21 Centre Nat Rech Scient PROCESS FOR PREPARING POLYMER ALLOY / ENZYMES
US10124512B2 (en) 2012-11-20 2018-11-13 Carbios Method for recycling plastic products
CA2947478C (en) 2014-05-16 2022-09-13 Carbios Process of recycling mixed pet plastic articles
US10723848B2 (en) 2015-06-12 2020-07-28 Carbios Masterbatch composition comprising a high concentration of biological entities
CA3024287C (en) 2016-05-19 2024-06-11 Carbios A process for degrading plastic products
KR102649944B1 (en) 2016-07-12 2024-03-22 까르비오 Novel esterases and uses thereof
CA3073791A1 (en) 2017-08-31 2019-03-07 Carbiolice Biodegradable polyester article comprising enzymes
MX2020002290A (en) 2017-08-31 2020-07-13 Carbios Liquid composition comprising biological entities and uses thereof.
US11851689B2 (en) 2018-07-27 2023-12-26 Carbios Esterases and uses thereof
FR3088070B1 (en) 2018-11-06 2021-11-26 Carbios ENZYMATIC DEGRADATION PROCESS OF POLYETHYLENE TEREPHTHALATE
FR3088069B1 (en) 2018-11-06 2021-11-26 Carbios INDUSTRIAL SCALE TEREPHTHALIC ACID PRODUCTION PROCESS
EP3838976B1 (en) 2019-12-19 2025-02-12 Carbios Process for degrading plastic products
MX2022007496A (en) 2019-12-19 2022-07-01 Carbios PROCESS TO DEGRADE PLASTIC PRODUCTS.
CN117866280A (en) 2019-12-19 2024-04-12 卡比奥斯公司 Foamed plastic composition

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018011281A1 (en) * 2016-07-12 2018-01-18 Carbios Novel esterases and uses thereof
WO2020021116A1 (en) * 2018-07-27 2020-01-30 Carbios Novel esterases and uses thereof
WO2020021117A1 (en) * 2018-07-27 2020-01-30 Carbios Novel esterases and uses thereof
WO2022243545A1 (en) * 2021-05-20 2022-11-24 Carbios Process for degrading a plastic product comprising at least one polyester
WO2023019222A1 (en) * 2021-08-11 2023-02-16 Biometis Technology, Inc. Enzymatic degradation of polyethylene terephthalate

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
DATABASE Geneseq [online] 19 March 2020 (2020-03-19), "Metagenome-derived cutinase variant (V17).", retrieved from EBI accession no. GSP:BHG01932 Database accession no. BHG01932 *
DATABASE Geneseq [online] 19 March 2020 (2020-03-19), "Metagenome-derived cutinase variant (V26).", retrieved from EBI accession no. GSP:BHG01941 Database accession no. BHG01941 *
DATABASE Geneseq [online] 19 March 2020 (2020-03-19), "Mutant esterase protein, SEQ ID NO:2.", retrieved from EBI accession no. GSP:BHG01024 Database accession no. BHG01024 *
DATABASE Geneseq [online] 22 December 2022 (2022-12-22), "PET degrading mutant cutinase enzyme F208I/D203C/S248C/V170I/Y92G.", retrieved from EBI accession no. GSP:BLZ66933 Database accession no. BLZ66933 *
DATABASE Geneseq [online] 23 March 2023 (2023-03-23), "Bhr-PETase S13L.", retrieved from EBI accession no. GSP:BML88009 Database accession no. BML88009 *
DATABASE Geneseq [online] 5 August 2021 (2021-08-05), "Cutinase protein mutant F208I/D203C/S248C/V170I/Y92G.", retrieved from EBI accession no. GSP:BJN91066 Database accession no. BJN91066 *
URBANEK ANETA K ET AL: "Biochemical properties and biotechnological applications of microbial enzymes involved in the degradation of polyester-type plastics", BIOCHIMICA ET BIOPHYSICA ACTA (BBA) - PROTEINS & PROTEOMICS, ELSEVIER, NETHERLANDS, vol. 1868, no. 2, 16 November 2019 (2019-11-16), XP086038547, ISSN: 1570-9639, [retrieved on 20191116], DOI: 10.1016/J.BBAPAP.2019.140315 *

Also Published As

Publication number Publication date
TW202511480A (en) 2025-03-16
WO2024236009A2 (en) 2024-11-21

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