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WO2022036003A3 - Improvement in lamp amplification in a diagnostic device - Google Patents

Improvement in lamp amplification in a diagnostic device Download PDF

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Publication number
WO2022036003A3
WO2022036003A3 PCT/US2021/045608 US2021045608W WO2022036003A3 WO 2022036003 A3 WO2022036003 A3 WO 2022036003A3 US 2021045608 W US2021045608 W US 2021045608W WO 2022036003 A3 WO2022036003 A3 WO 2022036003A3
Authority
WO
WIPO (PCT)
Prior art keywords
lamp
biological samples
pathogens
sensing
improvement
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2021/045608
Other languages
French (fr)
Other versions
WO2022036003A2 (en
Inventor
Joseph Carl GAITERI
Sha COLEMAN
Rixun Fang
Yuh-Min Chiang
Ronald Phillip Chiarello
Teresa Wang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alveo Technologies Inc
Original Assignee
Alveo Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alveo Technologies Inc filed Critical Alveo Technologies Inc
Priority to US17/670,193 priority Critical patent/US12472492B2/en
Publication of WO2022036003A2 publication Critical patent/WO2022036003A2/en
Publication of WO2022036003A3 publication Critical patent/WO2022036003A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present application is generally directed to systems, methods, and devices for diagnostics for sensing and/or identifying pathogens, genomic materials, proteins, and/or other small molecules or biomarkers, for example, using loop-mediated isothermal amplification (LAMP). In some implementations, a small footprint low cost device provides rapid and robust sensing and identification. Also disclosed herein are nucleic acid primers for use in the sensitive and specific detection of pathogens in biological samples by LAMP, which may be performed in the devices disclosed herein. The biological samples may be derived from patients including humans, plants, food, soil, contaminated surfaces, or animals such as livestock.
PCT/US2021/045608 2020-08-14 2021-08-11 Improvement in lamp amplification in a diagnostic device Ceased WO2022036003A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US17/670,193 US12472492B2 (en) 2020-08-14 2022-02-11 Systems and methods of sample depositing and testing

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202063066086P 2020-08-14 2020-08-14
US63/066,086 2020-08-14

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2021/045600 Continuation-In-Part WO2022035995A2 (en) 2020-08-14 2021-08-11 Systems and methods of sample depositing and testing

Related Child Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2021/045610 Continuation-In-Part WO2022036005A2 (en) 2020-08-14 2021-08-11 Nucleic acids for detection of infectious agents
US17/670,193 Continuation-In-Part US12472492B2 (en) 2020-08-14 2022-02-11 Systems and methods of sample depositing and testing

Publications (2)

Publication Number Publication Date
WO2022036003A2 WO2022036003A2 (en) 2022-02-17
WO2022036003A3 true WO2022036003A3 (en) 2022-03-10

Family

ID=80248130

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2021/045608 Ceased WO2022036003A2 (en) 2020-08-14 2021-08-11 Improvement in lamp amplification in a diagnostic device

Country Status (1)

Country Link
WO (1) WO2022036003A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119110850A (en) * 2022-12-21 2024-12-10 伊路米纳有限公司 Use of dry sodium hydroxide to denature double-stranded DNA

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020132008A1 (en) * 2018-12-20 2020-06-25 Alveo Technologies, Inc. Handheld impedance-based diagnostic test system for detecting analytes

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020132008A1 (en) * 2018-12-20 2020-06-25 Alveo Technologies, Inc. Handheld impedance-based diagnostic test system for detecting analytes

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PHILLIPS ET AL.: "Strand Displacement Probes Combined with Isothermal Nucleic Acid Amplification for Instrument-Free Detection from Complex Samples.", ANAL. CHEM., vol. 90, no. 11, 18 April 2018 (2018-04-18), pages 6580 - 6586, XP055915120 *

Also Published As

Publication number Publication date
WO2022036003A2 (en) 2022-02-17

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