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WO2022036005A3 - Nucleic acids for detection of infectious agents - Google Patents

Nucleic acids for detection of infectious agents Download PDF

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Publication number
WO2022036005A3
WO2022036005A3 PCT/US2021/045610 US2021045610W WO2022036005A3 WO 2022036005 A3 WO2022036005 A3 WO 2022036005A3 US 2021045610 W US2021045610 W US 2021045610W WO 2022036005 A3 WO2022036005 A3 WO 2022036005A3
Authority
WO
WIPO (PCT)
Prior art keywords
lamp
biological samples
detection
nucleic acids
pathogens
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2021/045610
Other languages
French (fr)
Other versions
WO2022036005A2 (en
Inventor
Joseph Carl GAITERI
Emma-Claire MCCARTHY
Adriana PATTERSON
Rixun Fang
Yuh-Min Chiang
Ronald Phillip Chiarello
Teresa Wang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alveo Technologies Inc
Original Assignee
Alveo Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alveo Technologies Inc filed Critical Alveo Technologies Inc
Priority to US17/670,193 priority Critical patent/US12472492B2/en
Publication of WO2022036005A2 publication Critical patent/WO2022036005A2/en
Publication of WO2022036005A3 publication Critical patent/WO2022036005A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
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    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
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    • B01L2300/041—Connecting closures to device or container
    • B01L2300/042—Caps; Plugs
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2400/0655—Valves, specific forms thereof with moving parts pinch valves
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    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present application is generally directed to systems, methods, and devices for diagnostics for sensing and/or identifying pathogens, genomic materials, proteins, and/or other small molecules or biomarkers, for example, using loop-mediated isothermal amplification (LAMP). In some implementations, a small footprint low cost device provides rapid and robust sensing and identification. Also disclosed herein are nucleic acid primers for use in the sensitive and specific detection of pathogens in biological samples by LAMP, which may be performed in the devices disclosed herein. The biological samples may be derived from patients including humans, plants, food, soil, contaminated surfaces, or animals such as livestock.
PCT/US2021/045610 2020-08-14 2021-08-11 Nucleic acids for detection of infectious agents Ceased WO2022036005A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US17/670,193 US12472492B2 (en) 2020-08-14 2022-02-11 Systems and methods of sample depositing and testing

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202063066040P 2020-08-14 2020-08-14
US63/066,040 2020-08-14

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2021/045608 Continuation-In-Part WO2022036003A2 (en) 2020-08-14 2021-08-11 Improvement in lamp amplification in a diagnostic device

Related Child Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2021/045596 Continuation-In-Part WO2022035992A1 (en) 2020-08-14 2021-08-11 Systems and methods of tracking infection potential
US17/670,193 Continuation-In-Part US12472492B2 (en) 2020-08-14 2022-02-11 Systems and methods of sample depositing and testing

Publications (2)

Publication Number Publication Date
WO2022036005A2 WO2022036005A2 (en) 2022-02-17
WO2022036005A3 true WO2022036005A3 (en) 2022-03-10

Family

ID=80248125

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2021/045610 Ceased WO2022036005A2 (en) 2020-08-14 2021-08-11 Nucleic acids for detection of infectious agents

Country Status (1)

Country Link
WO (1) WO2022036005A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150045254A1 (en) * 2013-08-07 2015-02-12 Xagenic Inc. Systems, methods and devices for electrochemical detection using helper oligonucleotides
US20190232282A1 (en) * 2016-09-23 2019-08-01 Alveo Technologies, Inc. Methods and compositions for detecting analytes

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150045254A1 (en) * 2013-08-07 2015-02-12 Xagenic Inc. Systems, methods and devices for electrochemical detection using helper oligonucleotides
US20190232282A1 (en) * 2016-09-23 2019-08-01 Alveo Technologies, Inc. Methods and compositions for detecting analytes

Also Published As

Publication number Publication date
WO2022036005A2 (en) 2022-02-17

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