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WO2022036003A3 - Amélioration de l'amplification lamp dans un dispositif de diagnostic - Google Patents

Amélioration de l'amplification lamp dans un dispositif de diagnostic Download PDF

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Publication number
WO2022036003A3
WO2022036003A3 PCT/US2021/045608 US2021045608W WO2022036003A3 WO 2022036003 A3 WO2022036003 A3 WO 2022036003A3 US 2021045608 W US2021045608 W US 2021045608W WO 2022036003 A3 WO2022036003 A3 WO 2022036003A3
Authority
WO
WIPO (PCT)
Prior art keywords
lamp
biological samples
pathogens
sensing
improvement
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2021/045608
Other languages
English (en)
Other versions
WO2022036003A2 (fr
Inventor
Joseph Carl GAITERI
Sha COLEMAN
Rixun Fang
Yuh-Min Chiang
Ronald Phillip Chiarello
Teresa Wang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alveo Technologies Inc
Original Assignee
Alveo Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alveo Technologies Inc filed Critical Alveo Technologies Inc
Priority to US17/670,193 priority Critical patent/US12472492B2/en
Publication of WO2022036003A2 publication Critical patent/WO2022036003A2/fr
Publication of WO2022036003A3 publication Critical patent/WO2022036003A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne de manière générale des systèmes, des méthodes et des dispositifs de diagnostic permettant de détecter et/ou d'identifier des pathogènes, des matériels génomiques, des protéines et/ou d'autres petites molécules ou biomarqueurs, par exemple, en utilisant une amplification isotherme à médiation par boucle (LAMP). Dans certains modes de réalisation, un dispositif de faible encombrement à faible coût permet d'obtenir une détection et une identification rapides et fiables. L'invention concerne également des amorces d'acide nucléique destinées à être utilisées dans la détection sensible et spécifique d'agents pathogènes dans des échantillons biologiques par LAMP, qui peuvent être mis en œuvre dans les dispositifs de l'invention. Les échantillons biologiques peuvent être prélevés sur des patients, notamment des individus humains, des plantes, des aliments, de la terre, des surfaces contaminées, ou des animaux tels que le bétail.
PCT/US2021/045608 2020-08-14 2021-08-11 Amélioration de l'amplification lamp dans un dispositif de diagnostic Ceased WO2022036003A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US17/670,193 US12472492B2 (en) 2020-08-14 2022-02-11 Systems and methods of sample depositing and testing

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202063066086P 2020-08-14 2020-08-14
US63/066,086 2020-08-14

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2021/045600 Continuation-In-Part WO2022035995A2 (fr) 2020-08-14 2021-08-11 Systèmes et procédés de dépôt et de test d'échantillons

Related Child Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2021/045610 Continuation-In-Part WO2022036005A2 (fr) 2020-08-14 2021-08-11 Acides nucléiques pour la détection d'agents infectieux
US17/670,193 Continuation-In-Part US12472492B2 (en) 2020-08-14 2022-02-11 Systems and methods of sample depositing and testing

Publications (2)

Publication Number Publication Date
WO2022036003A2 WO2022036003A2 (fr) 2022-02-17
WO2022036003A3 true WO2022036003A3 (fr) 2022-03-10

Family

ID=80248130

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2021/045608 Ceased WO2022036003A2 (fr) 2020-08-14 2021-08-11 Amélioration de l'amplification lamp dans un dispositif de diagnostic

Country Status (1)

Country Link
WO (1) WO2022036003A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3246530A1 (fr) * 2022-12-21 2024-06-27 Illumina Inc Utilisation d'hydroxyde de sodium séché pour dénaturer de l'adn double brin

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020132008A1 (fr) * 2018-12-20 2020-06-25 Alveo Technologies, Inc. Système de test de diagnostic basé sur l'impédance portatif pour la détection d'analytes

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020132008A1 (fr) * 2018-12-20 2020-06-25 Alveo Technologies, Inc. Système de test de diagnostic basé sur l'impédance portatif pour la détection d'analytes

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PHILLIPS ET AL.: "Strand Displacement Probes Combined with Isothermal Nucleic Acid Amplification for Instrument-Free Detection from Complex Samples.", ANAL. CHEM., vol. 90, no. 11, 18 April 2018 (2018-04-18), pages 6580 - 6586, XP055915120 *

Also Published As

Publication number Publication date
WO2022036003A2 (fr) 2022-02-17

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