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WO2019009592A3 - PARTICLE WITH UCST MATERIAL APPLIED TO THE SAME, AND METHOD FOR AMPLIFYING NUCLEIC ACID USING THE SAME - Google Patents

PARTICLE WITH UCST MATERIAL APPLIED TO THE SAME, AND METHOD FOR AMPLIFYING NUCLEIC ACID USING THE SAME Download PDF

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Publication number
WO2019009592A3
WO2019009592A3 PCT/KR2018/007523 KR2018007523W WO2019009592A3 WO 2019009592 A3 WO2019009592 A3 WO 2019009592A3 KR 2018007523 W KR2018007523 W KR 2018007523W WO 2019009592 A3 WO2019009592 A3 WO 2019009592A3
Authority
WO
WIPO (PCT)
Prior art keywords
same
primer
particle
ucst
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2018/007523
Other languages
French (fr)
Korean (ko)
Other versions
WO2019009592A2 (en
Inventor
김상경
김준선
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Korea Institute of Science and Technology KIST
Original Assignee
Korea Institute of Science and Technology KIST
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020180076483A external-priority patent/KR102088877B1/en
Application filed by Korea Institute of Science and Technology KIST filed Critical Korea Institute of Science and Technology KIST
Priority to US16/628,346 priority Critical patent/US20200239944A1/en
Priority to CN201880057430.3A priority patent/CN111356771A/en
Publication of WO2019009592A2 publication Critical patent/WO2019009592A2/en
Publication of WO2019009592A3 publication Critical patent/WO2019009592A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

In the present invention, when amplifying a nucleic acid by incorporating at least one primer among a forward primer and reverse primer and/or a probe in an upper critical solution temperature (UCST) particle, or when amplifying a nucleic acid by incorporating at least one primer among the forward primer and reverse primer and/or a probe in a UCST particle and fixing to a hydrogel fine particle, same the primer or probe contained in the UCST particle can be discharged within a certain temperature range. Accordingly, the formation of primer dimers can be prevented while also achieving excellent PCR amplification efficiency.
PCT/KR2018/007523 2017-07-04 2018-07-03 Particle with ucst material applied thereto, and nucleic acid amplification method using same Ceased WO2019009592A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US16/628,346 US20200239944A1 (en) 2017-07-04 2018-07-03 Particle with ucst material applied thereto, and nucleic acid amplification method using same
CN201880057430.3A CN111356771A (en) 2017-07-04 2018-07-03 Particles suitable for UCST material and nucleic acid amplification method using the same

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR10-2017-0084595 2017-07-04
KR20170084595 2017-07-04
KR10-2018-0076483 2018-07-02
KR1020180076483A KR102088877B1 (en) 2017-07-04 2018-07-02 Particle using ucst and method for amplifying nucleic acid using same

Publications (2)

Publication Number Publication Date
WO2019009592A2 WO2019009592A2 (en) 2019-01-10
WO2019009592A3 true WO2019009592A3 (en) 2019-04-11

Family

ID=64950195

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2018/007523 Ceased WO2019009592A2 (en) 2017-07-04 2018-07-03 Particle with ucst material applied thereto, and nucleic acid amplification method using same

Country Status (1)

Country Link
WO (1) WO2019009592A2 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6642000B1 (en) * 1999-11-12 2003-11-04 University Of Chicago PCR amplification on microarrays of gel immobilized oligonucleotides
US20100081175A1 (en) * 2007-05-25 2010-04-01 Beckman Coulter, Inc. Enzyme-containing gels and nucleic acid amplifying kits
KR101670232B1 (en) * 2013-10-28 2016-10-31 한국과학기술연구원 Porous matrix and method for producing thereof
KR101746055B1 (en) * 2016-05-03 2017-06-12 한국과학기술연구원 Core-shell porous matrix and PCR using the same

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6642000B1 (en) * 1999-11-12 2003-11-04 University Of Chicago PCR amplification on microarrays of gel immobilized oligonucleotides
US20100081175A1 (en) * 2007-05-25 2010-04-01 Beckman Coulter, Inc. Enzyme-containing gels and nucleic acid amplifying kits
KR101670232B1 (en) * 2013-10-28 2016-10-31 한국과학기술연구원 Porous matrix and method for producing thereof
KR101746055B1 (en) * 2016-05-03 2017-06-12 한국과학기술연구원 Core-shell porous matrix and PCR using the same

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DROBYSHEV, ALEXEI L.: "The role of DNA diffusion in solid phase polymerase chain reaction with gel-immobilized primers in planar and capillary microarray format", BIOMICROFLUIDICS, vol. 3, no. 4, 2009, pages 1 - 12, XP055590092 *

Also Published As

Publication number Publication date
WO2019009592A2 (en) 2019-01-10

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